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Complex enzyme capable of degrading cottonseed hulls, preparation method thereof using xylan for induction and application

A compound enzyme and cottonseed husk technology, which is applied in the fermentation preparation of compound enzyme and its application field, can solve the problems of not achieving the best synergistic treatment effect, great difference in catalytic conditions, and difficult to master the ratio

Inactive Publication Date: 2011-02-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the enzyme compounding technology is adopted, due to the different characteristics of the enzymes from different biological sources, the catalytic conditions are very different, and the ratio of various enzymes is difficult to master, so the best synergistic treatment effect cannot be achieved

Method used

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  • Complex enzyme capable of degrading cottonseed hulls, preparation method thereof using xylan for induction and application
  • Complex enzyme capable of degrading cottonseed hulls, preparation method thereof using xylan for induction and application
  • Complex enzyme capable of degrading cottonseed hulls, preparation method thereof using xylan for induction and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. Seed Culture of Trichoderma reesei

[0065] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N (125g / L sodium citrate dihydrate, 250g / L KH 2 PO 4 , 100g / L NH 4 NO 3 , 10g / LMgSO 4 ·7H 2 O, 250μg / L Biotin, 5g / L CaCl 2 2H 2 (2, 5mL / L trace element solution); Wherein, trace element solution contains 50g / L citric acid monohydrate, 50g / L ZnSO 4 ·7H 2O, 10g / L Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O, 2.5g / L CuSO 4 ·5H 2 O, 0.5g / L MnSO 4 ·H 2 O, 0.5g / L H 3 B0 3 , 0.5g / L Na 2 MoO 4 2H 2 O;

[0066] (2) Trichoderma reesei (T. reesei) Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0067] 2. Fermentation of Trichoderma reesei to produce enzymes

[0068] (1) Trichoderma reesei enzyme production medium: 0.1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, adding 2% xylan, pH5.0;

[0069] (2) Taking 10% inoculum size ...

Embodiment 2

[0106] 1. Seed Culture of Trichoderma reesei

[0107] (1) Trichoderma reesei seed medium: 1% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N;

[0108] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0109] 2. Fermentation of Trichoderma reesei to produce enzymes

[0110] (1) Trichoderma reesei enzyme production medium: 0.5% glucose, 0.1% peptone, 0.05% citric acid, 0.015% Tween 80, 2% Vogel's medium N, adding 2% xylan, pH5.0;

[0111] (2) Taking 5% inoculum size and culture without xylan as a control, culture at 30°C and 160r / min for 8 days to induce enzyme production;

[0112] (3) The fermented broth supernatant collected by filtration or centrifugation is exactly the compound enzyme liquid for hydrolyzing cottonseed hulls.

[0113] 3. Determination of cellulase activity, see Example 1 for details.

[0114] 4. Determination of xylanase activity, see Example 1 for det...

Embodiment 3

[0120] 1. Seed Culture of Trichoderma reesei

[0121] (1) Trichoderma reesei seed medium: 10g / L glucose, 1g / L peptone, citric acid buffer solution with a final concentration of 0.05M, 0.15g / L Tween 80, 1.4g / L (NH 4 ) 2 SO 4 , 2.0g / L KH 2 PO 4 , 0.3g / L urea, 0.4g / L CaCl 2 2H 2 O, 0.3g / L MgSO 4 ·7H 2 O, 0.005g / L FeSO 4 ·7H 2 O, 0.0016g / L MnSO 4 ·H 2 O, 0.0014g / LZnSO 4 ·7H 2 O, 0.0037g / L CoCl 2 ·6H 2 O's Mandels medium, pH4.8-5.0;

[0122] (2) Trichoderma reesei Rut C30 was used as the production strain, and cultured at pH 5.0, 30° C., 200 r / min for 36 hours.

[0123] 2. Fermentation of Trichoderma reesei to produce enzymes

[0124] (1) Trichoderma reesei enzyme production medium: 10g / L glucose, 1g / L peptone, a final concentration of 0.05M citrate buffer, 0.015% Tween 80, 1.4g / L (NH 4 ) 2 SO 4 , 2.0g / L KH 2 PO 4 , 0.3g / L urea, 0.4g / L CaCl 2 2H 2 O, 0.3g / L MgSO 4 ·7H 2 O, 0.005g / L FeSO 4 ·7H 2 O, 0.0016g / L MnSO 4 ·H 2 O, 0.0014g / LZnSO 4 ·7H 2 O, 0.0...

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Abstract

The invention relates to a complex enzyme capable of degrading cottonseed hulls, a preparation method thereof using xylan for induction and an application. The complex enzyme comprises the components of cellulolytic enzyme, xylanase and pectinase vigor. The preparation method comprises: inoculating a Trichoderma reesei seed into a seed culture medium; culturing for 24-48 hours under the condition that the temperature is 25-30 DEG C and the revolving speed is 120-250r / min; switching the cultured seed with the inoculation amount of 4-10% to a fermentation culture medium containing1-10% of xylan; in the culture process, adding xylan for further inducing for producing enzyme; culturing for 4-8 days under the conditions that the temperature is 25-30 DEG C and the revolving speed is 120-250r / min; and filtering, and centrifuging to obtain crude enzyme liquid. The complex enzyme prepared by the invention has high enzyme activity, is more suitable for the complex enzyme system degrading the cotton seed hulls, is safe, protects environment and has simple operation and strong controllability; and compared with normal alkali processing, the invention has moderate reaction condition, is friendly to environment and has good application prospect.

Description

technical field [0001] The invention belongs to the field of fermentation preparation and application of compound enzymes in microbial technology, and in particular relates to a compound enzyme capable of degrading cotton seed hulls and its preparation method and application induced by xylan. Background technique [0002] The chemical composition, color and shape of cotton seed hulls are different from cotton fibers and fabrics, which affect the uniformity of dyeing and the feel and appearance of fabrics. They must be removed before dyeing and finishing of fabrics. However, the structure of cottonseed hulls is dense and the composition is complex, which is the most difficult non-cellulose impurity to remove in the scouring process of cotton fabrics. Compared with other impurities in cotton fiber, its degradation usually requires more severe treatment conditions and longer time, so how to remove cotton seed hulls on the surface of cotton fabric has always been a difficult pro...

Claims

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Application Information

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IPC IPC(8): C12N9/20C12N9/50C12N9/26D06M101/06C12R1/885D06M16/00C12N9/42C12N9/00C12S11/00
Inventor 洪枫唐绿蓉曹张军
Owner DONGHUA UNIV
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