The present invention discloses a piglet intestinal health improving and weaning stress reducing creep feed and a preparation method thereof, and belongs to the field of feed. The feed comprises the following components: corn, puffed corn, flour, low-protein whey powder, 68% Peruvian steam fishmeal, plasma protein powder, fermented soybean meal, puffed soybeans, soybean oil, white sugar, glucose, an acidifier, an enzyme preparation, a flavor agent, compound probiotics, antimicrobial peptides, glutamine dipeptides, compound vitamins for piglets, compound organic trace elements for piglets, lysine, methionine, threonine, stone powder, calcium hydrogen phosphate, edible salt and a mildew preventive. The feed has a certain antigenicity, does not harm the intestinal tracts, promotes the development of the intestinal tracts, reduces the weaning stress, lays a solid foundation for a healthy and rapid growth in a late period, is free of antibiotics, not high in copper and zinc, and green, safe and pollution-free, prepared using crushing for twice and a low-temperature granulation technology, and free of nutrient damage, and enhances the digestion and absorption utilization. The nutritional characteristics and attractant characteristics of the creep feed are fully reflected.