Biological feed for creep feed and creep feed

A technology of biological feed and trough feed, applied in the field of animal feed, can solve the problem of high cost and achieve the effects of reducing waste, strong activity and reducing diarrhea rate

Active Publication Date: 2017-01-04
沈阳英大科技发展有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current creep feed usually contains many protein source feeds, and the cost is relatively high. Therefore, there is an urgent need for a low-cost, good palatability, and excellent creep effect.

Method used

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  • Biological feed for creep feed and creep feed
  • Biological feed for creep feed and creep feed
  • Biological feed for creep feed and creep feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, screening, breeding and identification of excellent bacterial strains

[0023] 1) Propagation of strains: collect fresh intestinal content samples from healthy pigs and inoculate them in LB liquid medium, and inoculate the culture medium in a 37°C incubator for 48 hours;

[0024] 2) Isolation and purification of Bacillus subtilis: heat the culture of expansion in step 1) in a water bath at 80°C for 20 minutes, then take out the treatment solution, dilute it, and spread it on LB medium, place it in a constant temperature incubator at 37°C Cultivate for 24 hours, pick out each colony with characteristic morphology from the culture, and then streak and separate until pure colonies are obtained. The results are checked by Gram staining, and the isolated suspected pure colonies are inoculated into test tubes Proliferate on a slant, and then store in a 4°C refrigerator for later use;

[0025] Colony morphology: the colony is round, with a smooth surface,...

Embodiment 22

[0030] Stress resistance and biological performance measurement of embodiment 2 2 excellent bacterial strains

[0031]Determination of biological properties and stress resistance of 2 strains: Bacillus subtilis CGMCC No.8148 and Lactobacillus plantarum CGMCC No.8150:

[0032] 1) Preparation of cultures of Bacillus subtilis CGMCC No.8148 and Bacillus licheniformis CGMCC No.2383: inoculate the slant strains stored in the refrigerator into LB seed medium for activation, and cultivate them at 35°C and 100rpm for 16 hours to obtain the product;

[0033] 2) Preparation of the culture of Lactobacillus plantarum CGMCC No.8150: inoculate the slant bacteria stored in the refrigerator into the MRS seed medium for activation, and cultivate at 30°C and 150rpm for 16 hours to obtain the product;

[0034] 3) Determination of acid resistance: Inoculate the cultures prepared above at 10% inoculum amount into artificial simulated gastric juice with pH values ​​of 2.0, 3.0, and 4.0, readjust the...

Embodiment 3

[0072] Embodiment 3 The screening of fermentation temperature and fermentation time

[0073] Under laboratory conditions, the screening of biological feed fermentation temperature was carried out. The fermentation raw materials contain: 0.1g / kg yeast, 0.1g / kg lactic acid bacteria, 0.1g / kg bacillus, 525g / kg puffed peeled soybean meal, 75g / kg corn flour, 225g / kg wheat bran, and 175g / kg water. The puffed peeled soybean meal is crushed before fermentation, the size of the crushing screen is 0.8×0.8 mm, the size of the corn grinding screen is 0.8×0.8 mm, and the size of the wheat bran grinding screen is 0.8×0.8 mm. Take 10 uniformly mixed samples of 200g each, put them evenly in a large petri dish, and then seal them with tape, and adjust the mold incubator to 15°C, 20°C, 25°C, 30°C, and 35°C respectively. Put two petri dishes under one temperature condition for fermentation and culture, and observe the progress of fermentation every day. If water vapor starts to be produced on th...

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Abstract

The invention discloses biological feed for creep feed. The biological feed is made of feed raw materials with a water content of 20-50% by sealing fermentation for 3-10 d at a temperature of 15-35 DEG C, wherein in a fermentation product, the number of survival bacteria is larger than or equal to 1*10<9>/g, and the content of organic acid is larger than or equal to 2%. The feed raw materials comprise, by weight, 0.05-0.2 g/kg of yeast, 0.05-0.2 g/kg of lactic acid bacteria, 0.05-0.2 g/kg of bacillus, 500-800 g/kg of puffed peeled bean pulp, 50-100 g/kg of corn flour and 100-300 g/kg of wheat bran. 5-10% of the biological feed is used for complete feed production of the creep feed and can replace fish meal, the prepared creep feed is balanced in nutrition and high in digestibility, the antibiotic application is reduced, the diarrhea rate and death rate of piglets can be effectively reduced, and the biological feed is of positive significance for daily gain of the piglets and environmental protection.

Description

technical field [0001] The invention belongs to the field of animal feed, in particular to a biological feed used for creep feed and the creep feed added with the biological feed. Background technique [0002] Biological feed, that is, fermented feed, is currently a hot spot in the research and development of animal husbandry workers at home and abroad. At present, it has been shown in many aspects such as the rational use of feed raw material resources in my country, the strengthening of feed and animal product safety production, the promotion of farm emission reduction, and the reduction of environmental pollution. Therefore, the development of biological feed has great strategic significance for domestic and foreign animal husbandry industries. It is reported that foreign bio-feed research has started since the 1980s, but it really developed after the 1990s. The research on bio-feed in my country started relatively late, starting in recent years. The research and develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/18A23K10/30A23K10/37A23K20/147A23K20/26A23K20/142
CPCY02P60/87
Inventor 张宗伟刘明忠胡忠宏张静沈美英孙建晨孟庆军姜倩倩
Owner 沈阳英大科技发展有限公司
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