Method for producing fermented feed containing probiotics for pigs by using microbial fermentation

A technology for microbial fermentation and fermented feed, applied in the field of fermented feed, can solve problems such as poor palatability, and achieve the effects of reducing anti-nutritional factors, promoting absorption, and the method is simple and quick

Inactive Publication Date: 2010-06-30
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to promote the absorption of phosphorus by animals, people add inorganic phosphates such as CaH to feed. 2 PO 4 As a supplement, but as a chemical product, inorganic phosphate is not only not palatable, but also an anti-nutritional factor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for producing pig probiotic fermented feed by microbial fermentation, comprising the following steps:

[0024] (1) Cultivate aerobic fungi:

[0025] ①Cultivate yeast, Aspergillus niger or Trichoderma on a slant, and shake flasks to prepare first-grade fungal seeds;

[0026] ②Using bran or / and rice bran as a medium, preparing secondary species of filamentous fungi at 25°C;

[0027] ③Cultivate the obtained secondary species of filamentous fungi in shallow pans for solid species of third-level fungi;

[0028] ④Third-grade fungal solid species are matured and then dried at 40°C as production strains;

[0029] (2) The cultivation of lactic acid bacillus:

[0030] Under the condition of 34°C, the lactic acid bacillus was cultivated in a first-level test tube, cultured in a second-level shaker, and put into a liquid fermenter for cultivation to obtain a liquid lactic acid bacillus production strain;

[0031] (3) Solid state culture:

[0032] ① fully mixing 3 part...

Embodiment 2

[0036] A method for producing pig probiotic fermented feed by microbial fermentation, comprising the following steps:

[0037] (1) Cultivate aerobic fungi:

[0038] ①Cultivate yeast, Aspergillus niger or Trichoderma on a slant, and shake flasks to prepare first-grade fungal seeds;

[0039] ②Using bran or / and rice bran as a culture medium, preparing secondary species of filamentous fungi at 28°C;

[0040] ③Cultivate the obtained secondary species of filamentous fungi in shallow pans for solid species of third-level fungi;

[0041] ④Third-grade fungal solid species are matured and then dried at 60°C as production strains;

[0042] (2) The cultivation of lactic acid bacillus:

[0043] Under the condition of 32°C, the lactic acid producing bacillus was cultivated in a first-stage test tube, cultured on a secondary shaker, and put into a liquid fermenter for cultivation to obtain a liquid lactobacillus producing strain;

[0044] (3) Solid state culture:

[0045] ① fully mixing...

Embodiment 3

[0049] A method for producing pig probiotic fermented feed by microbial fermentation, comprising the following steps:

[0050] (1) Cultivate aerobic fungi:

[0051] ①Cultivate yeast, Aspergillus niger or Trichoderma on a slant, and shake flasks to prepare first-grade fungal seeds;

[0052] ②Using bran or / and rice bran as a medium, prepare the secondary species of filamentous fungi at 27°C;

[0053] ③Cultivate the obtained secondary species of filamentous fungi in shallow pans for solid species of third-level fungi;

[0054] ④Third-level fungal solid species are matured and then dried at 55°C as production strains;

[0055] (2) The cultivation of lactic acid bacillus:

[0056] Under the condition of 36°C, the lactic acid bacillus was cultivated in a first-level test tube, cultured on a second-level shaker, and put into a liquid fermenter for cultivation to obtain a liquid lactic acid bacillus production strain;

[0057] (3) Solid state culture:

[0058] ① fully mixing 3 pa...

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PUM

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Abstract

The invention discloses a method for producing fermented feed containing probiotics for pigs by using microbial fermentation, comprising the following steps: (1) cultivating aerobic fungi for producing strains; (2) cultivating bacillus producing lactic acid; and (3) solid-state cultivating: 1) thoroughly mixing soya bean dregs, cotton dregs, vinegar residue with wheat bran and / or rice bran and adding water to prepare a mixture; 2) inoculating the producing strains prepared in the step (1) and the step (2) into the mixture; and 3) cultivating by stacking or bag stacking to obtain an antibiotics-free fermented feed. The method of the invention is simple and convenient. The products have stable properties and are easy for large-scale producing. Using the method to prepare the feed for the pigs can effectively reduce anti nutritional factors in the feed and the method plays a role in the gossypol reduction of the cotton dregs, thereby decreasing the input amount of inorganic phosphorus to the feed, increasing the utilization of organic phosphorus in the raw material of the feed, accelerating the absorption of nutrients in the feed for pigs and reducing the environment pollution due to the feed.

Description

technical field [0001] The invention relates to the technical field of fermented feed, in particular to a method for producing swine probiotic fermented feed by microbial fermentation. Background technique [0002] Due to the low utilization rate of pigs for feed digestion and the high use of cotton meal and other substances in commonly used pig feed, the content of anti-nutritional factors in feed is high. [0003] The plant raw materials used in pig feed contain a high content of phytic acid, which is an anti-nutritional factor commonly found in plant feed. Phytic acid phosphorus in phytic acid molecules is difficult to be utilized by pigs, and phytic acid can combine with mineral elements and proteins in feed to form stable complexes, thereby reducing the digestion and utilization of these substances. Phytate phosphorus accounts for 60% to 90% of the total plant phosphorus, and is the main storage form of phosphorus and inositol. Phosphorus is an inorganic element neces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/00C12N1/14
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
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