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Composite fermented protein feed and preparation method thereof

A protein feed, compound fermentation technology, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of polluted environment, pomace turns sour and smelly, low protein content, etc., to purify the environment and inhibit breeding , the effect of high protein content

Active Publication Date: 2019-02-01
宁波中瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At the same time, my country is the largest country in apple production, which leads to more than 1 million tons of apple pomace being discharged every year. In addition to the pomace of other fruits, the pomace of other fruits is as high as several million tons; Pomace has a high moisture content, low protein content, and rich content of soluble sugar, vitamins, minerals and cellulose. It is a good nutritional substrate for microorganisms. Therefore, if it is not treated in time, the pomace will soon become sour and smelly. polluted environment

Method used

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  • Composite fermented protein feed and preparation method thereof
  • Composite fermented protein feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method for compound fermented protein feed, comprising the steps of:

[0036] (1) Preparation of strains

[0037] The preparation method of Candida utilis strain (ACCC20060) comprises the steps of: inserting Candida utilis into wort medium, initial pH 6.8~7.0, 26°C, 200r / min shaking culture for 24~ 36h, the number of viable bacteria reached 2.0×10 8 More than cfu / g; Candida utilis medium components are as follows: yeast extract 3g / L, malt extract 3g / L, peptone 5g / L, glucose 10g / L, water to 1L;

[0038] The preparation method of Bacillus subtilis liquid (ACCC11025) comprises the following steps: insert Bacillus subtilis into Bacillus subtilis culture medium, initial pH 7.0, 35°C, 200r / min shaking culture for 24-36h, the number of viable bacteria reaches 1.8× 10 8More than cfu / g; Bacillus subtilis medium components are as follows: glucose 25.0g / L, peptone 5.0g / L, beef extract 3.0g / L, yeast extract powder 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, MnSO 4 ·H 2 O 0....

Embodiment 2

[0045] A preparation method of compound protein fermented feed, comprising the steps of:

[0046] (1) Preparation of strains

[0047] Step is with embodiment 1

[0048] (2) Preparation of compound fermentation broth

[0049] Step is with embodiment 1

[0050] (3) Preparation of compound protein fermented feed

[0051] Fully mix 50 parts of bean dregs (moisture content 30-40%), 25 parts of apple pomace (moisture content 25-30%), 15 parts of corn flour and 10 parts of bran to prepare the mixed material to be fermented; ) with the compound fermentation bacteria liquid prepared in ) to inoculate the above-mentioned mixed material to be fermented according to the inoculum amount of 15% by weight, and add water to adjust the final moisture content of the material to 50%, fully mix and place it still for 1-2 days; then put the material Put it into a packaging bag with a one-way outlet valve device, seal it, and ferment it statically at 28-35°C for 3-5 days to make a compound ferm...

Embodiment 3

[0053] A preparation method of compound protein fermented feed, comprising the following steps:

[0054] (1) Preparation of strains

[0055] Step is with embodiment 1

[0056] (2) Preparation of compound fermentation broth

[0057] Step is with embodiment 1

[0058] (3) Preparation of compound protein fermented feed

[0059] Fully mix 60 parts of bean dregs (moisture content 30-40%), 20 parts of apple pomace (moisture content 25-30%), 10 parts of corn flour and 10 parts of bran to prepare the mixed material to be fermented; ) to inoculate the above-mentioned mixed material to be fermented with the inoculum amount of 10% by weight, and add water to adjust the final moisture content of the material to 45%, mix well and leave it still for 1 to 2 days; then put the material into Put it into a packaging bag with a one-way outlet valve device, seal it, and ferment it statically at 28-35°C for 3-5 days to make a compound fermented protein feed.

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Abstract

The invention discloses a composite fermented protein feed and a preparation method thereof. The preparation method comprises the following steps: (1) uniformly mixing and stirring fermentation base materials including 40-70 parts of bean dregs, 20-30 parts of apple pomace, 5-15 parts of corn flour and 5-15 parts of bran for later use; (2) uniformly mixing a candida utilis liquid, a bacillus subtilis liquid and an aspergillus niger liquid to obtain a composite fermentation bacterial liquid; and (3) inoculating the fermentation base materials in the step (1) with the composite fermentation bacterial liquid in the step (2), conducting uniform mixing, and subjecting the obtained mixture to sealing, packaging and static fermentation so as to obtain the composite fermented protein feed. The bean dreg composite fermented protein feed prepared by the method does not need drying treatment, has low production cost and high probiotic content, has the effects of obviously improving animal intestinal tract health and improving animal immunity, and can also greatly reduce the pollution of the bean dregs and apple pomace to the environment and greatly relieve the current situation of the shortage of protein feed resources.

Description

technical field [0001] The invention relates to the field of biological fermentation feed processing, in particular to a compound fermented protein feed and a preparation method thereof. Background technique [0002] my country is a large animal husbandry and aquaculture country. With the high-speed growth of my country's aquaculture industry for many years, the shortage of feed resources, especially protein resources, is becoming more and more serious. According to the analysis of relevant experts from the Feed Research Institute of the Chinese Academy of Agricultural Sciences, by 2020, the annual supply and demand gap of protein feed in my country will reach 48 million tons. Therefore, it is difficult to meet the needs of the rapid development of aquaculture by utilizing existing conventional vegetable protein raw materials (such as soybean meal, rapeseed meal, etc.) and animal protein raw materials. Therefore, according to my country's national conditions, how to expand ...

Claims

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Application Information

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IPC IPC(8): A23K10/37A23K10/30A23K10/12C12N1/16C12N1/14C12N1/20C12R1/72C12R1/125C12R1/685
CPCA23K10/12A23K10/30A23K10/37C12N1/14C12N1/16C12N1/20Y02P60/87
Inventor 方银兵
Owner 宁波中瑞生物科技有限公司
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