Composite fermented protein feed and preparation method thereof
A protein feed, compound fermentation technology, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of polluted environment, pomace turns sour and smelly, low protein content, etc., to purify the environment and inhibit breeding , the effect of high protein content
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[0034] Example 1
[0035] A method for preparing compound fermented protein feed includes the following steps:
[0036] (1) Preparation of strains
[0037] The preparation method of Candida utilis liquid (ACCC20060) includes the following steps: inserting Candida utilis into the wort medium, initial pH 6.8~7.0, 26℃, 200r / min shaking culture 24~ 36h, the number of viable bacteria reaches 2.0×10 8 cfu / g or more; the components of Candida utilis culture medium are as follows: yeast extract 3g / L, malt extract 3g / L, peptone 5g / L, glucose 10g / L, and the volume of water is set to 1L;
[0038] The preparation method of Bacillus subtilis liquid (ACCC11025) includes the following steps: insert Bacillus subtilis into Bacillus subtilis culture medium, initial pH 7.0, 35℃, 200r / min shaking culture for 24~36h, the number of viable bacteria reaches 1.8× 10 8 Cfu / g or more; Bacillus subtilis medium components are as follows: glucose 25.0g / L, peptone 5.0g / L, beef extract 3.0g / L, yeast extract 1.0g / L,...
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[0044] Example 2
[0045] A preparation method of compound protein fermented feed includes the following steps:
[0046] (1) Preparation of strains
[0047] The steps are the same as in Example 1
[0048] (2) Preparation of compound fermentation broth
[0049] The steps are the same as in Example 1
[0050] (3) Preparation of compound protein fermented feed
[0051] Mix 50 parts of bean dregs (moisture content 30-40%), 25 parts of apple pomace (moisture content 25-30%), 15 parts of corn flour and 10 parts of bran thoroughly to prepare the mixture to be fermented; the step (2) The compound fermentation broth prepared in) is used to inoculate the above-mentioned mixture to be fermented at an inoculum of 15% by weight, and water is added to adjust the final moisture content of the material to 50%, and the material is fully mixed and placed in a static state for 1 to 2 days; Put it into a packaging bag with a one-way air outlet valve device and seal it, and ferment it at 28-35°C for 3 to 5 ...
Example Embodiment
[0052] Example 3
[0053] A preparation method of compound protein fermented feed includes the following steps:
[0054] (1) Preparation of strains
[0055] The steps are the same as in Example 1
[0056] (2) Preparation of compound fermentation broth
[0057] The steps are the same as in Example 1
[0058] (3) Preparation of compound protein fermented feed
[0059] Mix 60 parts of bean dregs (moisture content 30-40%), 20 parts of apple pomace (moisture content 25-30%), 10 parts of corn flour and 10 parts of bran thoroughly to prepare the mixture to be fermented; The compound fermentation broth prepared in) is used to inoculate the above-mentioned mixture to be fermented at an inoculum of 10% by weight, and water is added to adjust the final moisture content of the material to 45%, and the material is fully mixed and placed in a static state for 1 to 2 days; Put it into a packaging bag with a one-way air outlet valve device and seal it, and ferment it at 28-35°C for 3 to 5 days to make ...
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