Aspergillus niger strain and use thereof
A strain of Aspergillus niger, the technology of Aspergillus niger, applied in the direction of application, fungi, enzymes, etc., can solve the problems of high cost, difficulty in meeting feed ingredients, incomplete enzyme system, etc., achieve low production cost, good economic benefits, and improve digestibility Effect
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[0020] Example 1. Obtaining and functional verification of Aspergillus niger strain A46 strain with high yield of compound enzyme for feed
[0021] 1. Acquisition of Aspergillus niger strain A46 strain with high yield of compound enzyme for feed
[0022] 1. Strain mutagenesis
[0023] Aspergillus niger strain (Aspergillus niger) CGMCC 3.316 (purchased from the General Microorganism Center of China Microbiological Culture Collection Management Committee) was inoculated on the potato dextrose medium (PDA) slant, and cultured at 28 ° C for 3 to 4 days until the spores matured. Wash the well-grown spores with an appropriate amount of sterile physiological saline, transfer them into a conical flask with glass beads, and vibrate on a shaker at 28°C for 4-5 hours to break up the spores and activate them. Use four layers of sterile lens paper to filter and remove mycelium fragments, dilute the spore filtrate with an appropriate amount of normal saline, check the spore concentration w...
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