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A method for reducing the tobacco sheet pectin content through a three-step manner

A technology of pectin and pectinase, which is applied in the field of degrading the pectin content of tobacco flakes in a three-step method, can solve the problems of poor pertinence of pectin degradation, difficulty in controlling the degradation process, and insufficient degradation, etc.

Inactive Publication Date: 2017-11-10
CHINA TOBACCO HENAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complexity and diversity of the pectin structure in the tobacco sheet, pectinases in the prior art generally have the defects that the degradation of pectin in the tobacco sheet is not targeted, the degradation is not sufficient, and the degradation process is difficult to control. Urgent need to explore new pectinases and their application in tobacco sheet processing

Method used

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  • A method for reducing the tobacco sheet pectin content through a three-step manner

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The pectinase prepared by the fermentation of Rhizopus chinensis provided in this embodiment is prepared through the following production steps.

[0062] (1) Strain activation, pick the fermentation strain from the preservation medium, inoculate it into the activation medium, and activate it for about 3 days;

[0063] The fermentation strain is specifically Rhizopus chinensis with the preservation number of CICC 41051 deposited by the China Industrial Microorganism Collection (CICC) under the Institute of Microbiology of the Chinese Academy of Sciences (L188) of the Heilongjiang Academy of Light Industry Science (L188). Buy

[0064] The activation medium is a PDA medium; when strains are picked from the preservation medium and then the activation medium is inoculated, the streaking method is used for inoculation;

[0065] The PDA medium is prepared according to a conventional method, specifically: potato extract 1.0L, glucose 20.0g, agar 15.0g, natural pH;

[0066] The method fo...

Embodiment 2

[0078] The crude enzyme solution prepared in Example 1 was applied to the three positions of the primary leaching liquid, the secondary leaching liquid and the mixed slurry on the Henan Zhongyan tobacco sheet production line using the "three-step method" (the production process flow chart is as follows: figure 1 Shown), used to degrade the pectin in the leaching liquid and mixed pulp, the specific usage method is:

[0079] The crude enzyme solution of pectinase prepared in Example 1 (the enzyme activity is 2986.87 U / mL, theoretically, the crude enzyme solution of any enzyme activity prepared in Example 1 can be used for degrading pectin applications, but for certainty Better degradation conditions and actual improvement effects on tobacco, so only the crude enzyme solution prepared in Example 1 with an enzyme activity of 2986.87 U / mL is used as an example for experiment) to properly dilute the diluted crude enzyme solution according to different volumes Ratio (ratio of leaching s...

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Abstract

The invention belongs to the technical field of tobacco preparation, and particularly relates to a method for reducing the tobacco sheet pectin content through a three-step manner. A specific strain is subjected to fermentation firstly, a crude enzyme solution having maximum pectinase activity is prepared, and the crude enzyme solution is directly used in a tobacco sheet production process by adopting a three-step manner, thus reducing the pectin content of a sheet product. Through optimizing a processing process, an optimum degrading effect is obtained, thus improving tobacco quality. The enzyme activity of the crude enzyme solution prepared by fermentation of the specific strain can be as high as 2986.87 U / mL, and application potential is good. After the crude enzyme solution is further used for tobacco sheet raw material processing, the pectin content of a tobacco sheet is finally reduced. The three-step manner is a manner for performing enzymatic hydrolysis in different processing stages of a tobacco raw material leachate in a tobacco sheet processing process, and the pectinase prepared by fermentation is added separately in different process stages so that the pectin content of a tobacco sheet finished product is reduced.

Description

Technical field [0001] The invention belongs to the technical field of tobacco preparation, and specifically relates to a three-step method for degrading the pectin content of tobacco flakes. Background technique [0002] Paper-Process Reconstituted Tobacco (Paper-Process Reconstituted Tobacco) is a product of recycling tobacco resources. It uses some tobacco waste materials and leftovers, such as tobacco stems, tobacco leaf fragments, tobacco dust, etc., as raw materials, through extraction, concentration, separation, pulping, refining, papermaking, drying, and flavoring processes to make tobacco leaf flakes , Used as a cigarette filler. Paper-making tobacco flakes not only have advantages in improving the structural strength, burning performance of tobacco products, and reducing the amount of nicotine and tar in cigarette smoke, but also reduce the unit consumption of tobacco leaves and save production costs. It can be said that the tobacco industry is the most in recent years...

Claims

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Application Information

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IPC IPC(8): C12N9/18C12N9/24C12N9/88A24B3/14C12R1/66
CPCA24B3/14C12N9/18C12N9/2402C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002
Inventor 郝辉张俊岭马宇平许春平陈芝飞刘绍锋田海英王充余金伟冉盼盼姜宇
Owner CHINA TOBACCO HENAN IND
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