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High-activity composite enzyme for feed

A compound enzyme for feed, high-activity technology, applied in the direction of enzyme, application, lyase, etc., can solve the problems of popularization and application obstacles, affecting the effect of use, growth and synthesis difficulties, etc., to enhance the body's functionality and high vitality , Adjust and repair the effects of body functions

Active Publication Date: 2014-04-02
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage is that the cost is high, and it is not easy to mix the enzyme preparation with high enzyme activity and low dosage with a large amount of feed when used, which seriously affects the effect of use, which increases the cost of use virtually, and makes many operators It is prohibitive and brings obstacles to promotion and application
2. Multi-strain mixed fermentation, natural complementary enzyme system method: use several strains for mixed fermentation, and the complementary enzyme production method to obtain a compound enzyme product that is closer to the compound method, and then only need a little adjustment to get the product, but multi-bacteria The technical difficulty of species fermentation is very large, because of the respective physiological characteristics of different strains, the mixed culture will inevitably affect each other, and it is difficult to make them grow on the same medium and synthesize different enzymes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Feed compound enzyme enzyme activity assay

[0043] 1) Definition of enzyme activity unit:

[0044] Protease enzyme activity unit definition: under the conditions of 40°C and pH 5.5, the amount of enzyme that hydrolyzes casein for 1 minute to produce 1 μmol of tyrosine is 1 enzyme activity unit, expressed in U / ml.

[0045] Definition of mannanase activity unit: at 40°C and pH 5.5, the amount of enzyme required to hydrolyze and release reducing sugar equivalent to 1 μmol mannose from a locust bean gum solution with a concentration of 5 mg / ml in 1 min is 1 enzyme Vitality unit, expressed in U / ml.

[0046] Definition of α-galactosidase activity unit: at 40°C and pH 5.5, the amount of enzyme needed to decompose p-nitrophenol-α-D-galactopyranose into 1 μmol p-nitrophenol in 1 minute is 1 α- Galactosidase enzyme activity unit, expressed in U / ml.

[0047] Definition of pectinase activity unit: at 40°C and pH 5.5, the amount of enzyme required to hydrolyze and r...

Embodiment 2

[0051] A method for preparing a compound enzyme for high-activity feed comprises the following steps:

[0052] (1) Activation of strains

[0053] Inoculate the well-preserved slant strain of Aspergillus niger DM-18 on the slant medium, culture at 30°C for 42 hours to activate the strain, and activate it 3 times;

[0054] The slant medium consists of: casein 4g, dipotassium hydrogen phosphate 2g, magnesium chloride 0.6g, potassium chloride 0.8g, ferrous sulfate 0.02g, glucose 20g, agar 20g, Chinese herbal medicine powder 12g, distilled water 1000mL, pH value 5.8 , sterilized at 121°C for 20 minutes.

[0055] The preparation method of described Chinese herbal medicine powder is as follows:

[0056] Weigh 25 parts of Radix Astragali; 15 parts of Codonopsis; 12 parts of Bupleurum; Keep it for 3 hours, add a mixture of ethanol and propanol which is 3 times the weight of the mixed material, control the temperature to 70° C., keep it for 4 hours, and filter; the filtrate is concen...

Embodiment 3

[0077] The thermostability analysis of embodiment 3 high activity compound enzyme for feed

[0078] The thermal stability of the compound enzyme for feed was analyzed, and the crude enzyme solution of Example 4 was placed at 30°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, and 75°C respectively , take samples every 10 minutes to measure the enzyme activity of each component enzyme in the feed compound enzyme. At 30°C, 40°C, 45°C, and 50°C, the enzyme activity of each composition did not decrease for 60 minutes. At 55°C, 60°C and 65°C, the enzyme activity of each composition decreased to 95% of the original enzyme activity in 30 minutes, and the enzyme activity of each composition decreased to 85% in 60 minutes. At 70°C, the enzymatic activity of each constituent enzyme decreased to 90% of the original enzyme activity in 30 minutes, and the enzyme activity of each constituent enzyme decreased to 85% in 60 minutes. At 75°C, the enzyme activity of each component drops to 85% of ...

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Abstract

The invention discloses a high-activity composite enzyme for feed, belonging to the technical field of preparation of an enzyme preparation for feed. An Aspergillus niger DM-18 strain for a high-yield composite enzyme for feed, which is used as an initial strain, is subjected to liquid submerged mixed fermentation in an optimized culture medium under specific fermentation conditions by an improve fermentation technique to obtain the composite enzyme for feed, which has the advantages of complete enzymatic system, high enzyme activity, high temperature resistance and wide pH value resistance range. The crude enzyme fermentation liquid of the high-activity composite enzyme for feed contains multiple enzymes, wherein the proteinase activity is 6500-6700 U / ml, the mannase activity is 1500-1700 U / ml, the alpha-galactase activity is 1200-1300 U / ml, and the pectinase activity is 1000-1200 U / ml. When the crude enzyme fermentation liquid is subjected to the heat stability test and pH stability test, the test result indicates that all the enzymes have higher enzyme activity (up to higher than 80%) at 30-75 DEG C under the pH value of 2-7.

Description

technical field [0001] The invention relates to an enzyme preparation for feed, in particular to a compound enzyme with high activity for feed. Background technique [0002] A large number of studies at home and abroad have shown that adding enzyme preparations to feed can promote the digestion and absorption of nutrients by animals, improve feed utilization, and enhance animal disease resistance. Its main functions are as follows: (1) Supplement animal Insufficient endogenous enzymes improve feed digestibility and utilization. The enzyme preparation promotes the digestion and absorption of carbohydrates and other nutrients in the digestive tract, improves the conversion efficiency of feed, and is beneficial to the growth of animals. (2) Eliminate anti-nutritional factors in feed, reduce the viscosity of chyme in the digestive tract of animals, improve digestive function, and facilitate the absorption of nutrients. (3) Improve the distribution of intestinal flora and impro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/40C12N9/18C12N9/26C12N9/88C12N9/62A23K1/165C12R1/685
CPCA23K20/189C12N9/18C12N9/2465C12N9/2488C12N9/50C12N9/88C12Y302/01022
Inventor 李洪兵李海清胡永明易继云向左东
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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