Method for producing pectinase biocontrol inducer by using apple waste residues

A technology of apple waste residue and pectinase, applied in the field of microbial pesticides and environmental protection, can solve problems such as few reports on plant disease resistance

Inactive Publication Date: 2012-07-11
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

However, the above-mentioned pectinases are all derived from plant pathogenic bacteria, and there are few r

Method used

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  • Method for producing pectinase biocontrol inducer by using apple waste residues
  • Method for producing pectinase biocontrol inducer by using apple waste residues
  • Method for producing pectinase biocontrol inducer by using apple waste residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Different strains use apple pomace to produce pectinase experiment:

[0014] Using the above-mentioned apple pomace medium formula, experiments were carried out on Penicillium oxalicum, Aspergillus niger, Mucor tallowa, Botrytis cinerea, etc., and the pectinase production by each bacteria using apple pomace is shown in Table 1.

[0015] Table 1

[0016]

Embodiment 2

[0018] Effects of different nitrogen sources on pectinase production by Penicillium oxalicum BZH-2002:

[0019] The strain Penicillium oxalicum BZH-2002 was stored in PDA medium at 4°C in the refrigerator, and transferred once every six months. Transfer to a freshly configured incline before use for seed preparation. The prepared seeds were inoculated into the solid medium in the enamel dish at a ratio of 10% to carry out the enzyme production experiment: various inorganic nitrogen sources and organic nitrogen sources were added to prepare the enzyme production medium, and the proportion of each medium was apple pomace 25 % (W / W), nitrogen source 0.55% (W / W), moisture about 74% (W / W), a small amount of phosphate. Inoculate Penicillium oxalicum BZH-2002 for solid-state fermentation, and the results are shown in Table 2.

[0020] table 3

[0021]

example 3

[0023] Yield of Pectinase in Solid-state Fermentation of Different Waste Residues

[0024] Use sunflower dish, sugar beet pomace, apple pomace, orange peel, banana peel as carbon source, and ammonium sulfate as nitrogen source to prepare enzyme production medium. The proportion (W / W) of each medium is 25% of waste residue, 2%, water 73%, a little phosphate. Inoculate Penicillium oxalicum BZH-2002 for solid-state fermentation, and the results are shown in Table 3.

[0025] table 3

[0026]

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Abstract

The invention relates to a method for producing a pectinase biocontrol inducer by using apple waste residues as main raw materials and belongs to the technical field of microbial pesticides and environmental protection. A bacterial strain selected and used for fermenting pectinase is high-yield pectinase, and a preparation is used for inducing penicllium oxalicum BZH-2002 with obvious disease-resistant effect. The production method of the pectinase biocontrol inducer comprises five steps of strain activation and storage, seed preparation, solid state fermentation, post-processing technology and determination of pectinase activity; and according to the production method, the apple waste residues are used as the main carbon sources of a fermentation medium, and an inorganic ammonium sulfate is used as nitrogen source. The technical scheme of the invention provides a new way for the resource utilization of the apple waste residues; and the produced pectinase biocontrol inducer can be applied to fields and has better prevention and cure effect on multiple plant diseases.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and environmental protection. Background technique [0002] The planting area and output of apples in my country rank first in the world. With the expansion of apple deep processing scale, a large amount of apple pomace has been produced. However, apple pomace is easily corrupted and becomes smelly, which seriously pollutes the environment. The treatment of apple pomace has always been one of the important problems that plague the development of apple processing enterprises. Apple pomace is mainly composed of apple peel, fruit core and part of the pulp, and has high nutritional value. It is a precious resource with a wide range of uses, high use value and economic value. However, at present, except for a small amount of apple pomace being used for feed and deep processing, most of it is abandoned, which not only causes waste of resources, but also seriously pollutes the environment....

Claims

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Application Information

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IPC IPC(8): C12N9/26A01N63/04A01P1/00C12R1/80
Inventor 彭霞薇高莎宿燕明周巍白志辉
Owner BEIJING FORESTRY UNIVERSITY
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