61 results about "Enzybiotics" patented technology

A method of forming an array of proteins selected from antigens or antibodies; said method comprising the steps of (i) expressing in a recombinant cell, a fusion protein which comprises either (a) an antigen or (b) an antibody binding protein, fused to a peptide having up to 50 amino acids, which peptide comprises amino acid sequence of SEQ ID NO 1 LX₁X₂IX₃X₄X₅X₆KX₇X₈X₉X₁₀ (SEQ ID NO 1) where X₁ is a naturally occurring amino acid, X₂ is any naturally occurring amino acid other than leucine, valine, isoleucine, tryptophan, phenylalanine or tyrosine, X₃ is phenylalanine or leucine, X₄ is glutamine or asparagine, X₅ is alanine, glycine, serine or threonine, X₆ is glycine or methionine, X₇ is isoleucine, methionine or valine, X₈ is glutamine, leucine, valine, tyrosine or isoleucine, X₉ is tryptophan, tyrosine, valine, phenylalanine, leucine or isoleucine and X₁₀ is any naturally occurring amino acid other than asparagine or glutamine; where said peptide is capable of being biotinylated by a biotin ligase at the lysine residue adjacent to X₆; (ii) biotinylating said peptide of the fusion protein at the lysine residue adjacent X₆; (iii) isolating the biotinylated fusion protein; (iv) applying the biotinylated fusion protein to an avidin or streptavidin coated non-porous support; (v) forming an array of at least three different proteins on the support by either (a) where the fusion protein comprises an antigen, carrying out steps (i) to (iv) the desired number of times to form an antigen array; or (b) where the fusion protein comprises an antibody binding protein, applying to said protein, either prior to or after step (iv) a plurality of different antibodies or binding fragments thereof.

The invention relates to a method for preparing an acetylcholinesterase fluorescence biosensor. The invention solves the following problems: the existing enzyme biosensor is prone to being affected by environmental influence so that the enzyme losses activity thus resulting false- positive; the existing biosensor is not allowed for repeated use due to irreversible reactions, in which enzymes are expensive, so that the cost of the equipment is excessively high; the selectivity is low, etc. The method comprises the following steps: firstly, carrying out the extraction separation and purification on acetylcholinesterase in crucian carp brain; then, carrying out the immobilization on the acetylcholinesterase in the crucian carp brain by chitosan; preparing immobilized acetylcholinesterase membranes, namely, obtaining the acetylcholinesterase fluorescence biosensor. The acetylcholinesterase fluorescence biosensor prepared by the invention dispenses with neither external power connection nor reference electrodes, and has the advantages of small size, light weight, high response speed, electromagnetic interference resistance, corrosion resistance, high sensitivity, wide measurement bandwidth, convenient operation, low cost and the like, therefore, the invention is applicable to the rapid detection of organophosphorus pesticide in food.

The invention relates to an oral enteric-coated preparation of total flavonoids of herba epimedii and an application thereof, belonging to the technical field of medicines. The oral enteric-coated preparation is an enteric capsule, an enteric pill or an enteric tablet and the like capable of being orally taken, which can be prepared from total flavonoids of herba epimedii, hydrolytic enzyme, a biological adhesion agent and other common auxiliary materials in pharmaceutics. According to a bionic enzymolysis oral administration system of the total flavonoids of herba epimedii developed by the technical scheme, the total flavonoids of herba epimedii can be subjected to complete enzymolysis to form a secondary glycoside or aglycone form before intestinal emptying under an in-vivo intestinal environment (37 DEG C and pH 5.2-7.4); the retention time of the administration system on the intestinal absorption site is prolonged by adding the biological adhesion agent, and thus 'real-time enzymolysis and real-time absorption' of medicines are achieved, full enzymolysis of herba epimedii flavonoid glycoside can be further guaranteed, the bioavailability is improved, and more effective and more stable treatment means are provided for osteoporosis and the like.

The invention discloses a gene engineering strain capable of safely and efficiently producing Phenazino-1-carboxylic acid. The gene engineering strain is Pseudomonas aeruginosa with a collection number of CCTCC No. M2015040. The gene engineering strain is prepared according to the following steps: performing pathogenic gene knockout on a strain PA1201; then, performing Shenqinmycin metabolizing gene knockout and metabolize metabolizing gene knockout; then, performing point mutation to reduce the bioactivity of PheA; performing isozyme substitution method to reduce the bioactivity of chorismic acid/ pyruvic acid lyase; increasing a phzC gene copy number in a genome; replacing an aroG promoter with a strong promoter Ptac so as to enhance the gene expression level of DAHP synthase; and enhancing the Phenazino-1-carboxylic acid biosynthetic gene cluster expression level and Phenazino-1-carboxylic acid efflux pump MexGHI-OpmD expression level by a promoter replacement method. The obtained strain is capable of safely and economically producing Phenazino-1-carboxylic acid with superhigh yield.

The invention discloses a synechocystis-6803 genetically engineered bacterium capable of producing 3-hydroxypropionic acid, and a construction method and application thereof. The construction method is as follows: a malonyl coenzyme A reductase gene in orange chloroflexus is cloned into synechocystis 6803; and acetylcoenzyme A carboxylase, biotin acylase and an NAD(P) transhydrogenase gene in the synechocystis 6803 are expressed. The synechocystis 6803 is transformed through a synthetic biology method to obtain the synechocystis-6803 genetically engineered bacterium capable of producing the 3-hydroxypropionic acid. The experiments prove that the final yield of the 3-HP (3-hydroxypropionic acid) of the genetically engineered bacterium is up to 837.18mg/L, which is of great theoretical and practical significances for the production of the 3-HP through photosynthetic microorganisms.

The invention relates to the field of prevention and treatment of oral diseases, in particular to an oral care agent with an antibacterial effect, which comprises lysozyme and montmorillonite. The invention relates to a lysozyme biological toothpaste, which comprises 0.4% of lysozyme, 1% of montmorillonite, an abrasive, a wetting agent, an adhesive, a surfactant, a preservative, a sweetening agent, a thickening agent, a foaming agent and the balance of deionized water. The lysozyme biological toothpaste has an antibacterial effect on oral pathogenic bacteria, is wide in antibacterial spectrumand high in antibacterial rate, can efficiently inhibit growth of the oral pathogenic bacteria in a short time, is relatively low in minimum antibacterial concentration, and does not generate drug-resistant strains of the oral pathogenic bacteria after being used for a long time; the prepared toothpaste can be prevented from being affected by denaturation of lysozyme by substances such as a foaming agent, a binder, a sweetening agent and a preservative, and the activity of biological lysozyme is prevented from being damaged or reduced, so that the product plays a role in inhibiting oral pathogenic bacteria, can effectively play a role in preventing caries and periodontitis, and has unique advantages and wide application prospects.

The invention discloses a ZnO/enzyme biosensor and preparation method, and aims to solve the problems that the present device and method have complex process, strict requirement for the experimental environment, and poor repeatability. The ZnO/enzyme biosensor includes a supporting base, a conductive thin film layer, an insulation wrapping layer, a ZnO nanometer material layer and bio-enzyme molecules; and the preparation method is realized through the following steps: firstly the conductive thin film layer is evaporated on the supporting base to form a substrate, the ZnO nanometer material layer grows on a rectangle I with area of axb at one end of the substrate, the bio-enzyme molecules are fixed in the ZnO nanometer material layer, the other end of the substrate is reserved for another rectangle II with the area of cxd, and the insulation wrapping layer wraps the middle section of the substrate. According to the invention, the preparation process is simple, and the pollution is avoided, the raw material is abundant and cheap, so that the preparation method is particularly suitable for preparing low-cost ZnO/ enzyme biosensors on a large scale.

The invention discloses a Pantoea amidase, a gene, a vector, an engineering bacterium and an application thereof in preparation of 1-cyancyclohexylacetic acid through biological catalysis of 1-cyancyclohexylacetamide. The amino acid sequence of the amidase is represented by SEQ ID NO.1. A synthesis technology of 1-cyancyclohexylacetic acid through biological catalysis by using the amidase is reported in the invention for the first time, and the technology has the substantial advantages of small catalyst dosage (2g/L), high substrate concentration (100g/L), short reaction time (20min), and high product conversion rate reaching 100%.

The invention provides a method for separating L-ornithine from an L-ornithine conversion solution prepared by using an enzyme biotechnology and forming L-ornithine hydrochloride. The L-ornithine conversion solution is obtained by converting arginase into arginine. The method comprises the concrete steps of firstly, treating the conversion solution by using a nanofiltration membrane 1 with the molecular weight cutoff of 300-1000Da to obtain filtrate 1; then, treating the filtrate 1 by using a nanofiltration membrane 2 with the molecular weight cutoff of 50-100Da to obtain filtrate 2 containing L-ornithine; and after adding acid to form salt, decoloring by using activated carbon, and separating a crystal to obtain the L-ornithine hydrochloride. The traditional ethanol extraction method is replaced with the method provided by the invention, so that the L-ornithine hydrochloride is extracted without absolute ethyl alcohol, furthermore, an anti-explosion workshop does not need to be built by a production enterprise of the L-ornithine hydrochloride, the technical threshold for producing the L-ornithine hydrochloride is reduced, and the method is also safe and reliable; in addition, a urea solution generated in the process that the L-ornithine hydrochloride is prepared by using the method provided by the invention can be used as a fertilizer to be directly used for agricultural production.

The invention relates to a method for producing a pectinase biocontrol inducer by using apple waste residues as main raw materials and belongs to the technical field of microbial pesticides and environmental protection. A bacterial strain selected and used for fermenting pectinase is high-yield pectinase, and a preparation is used for inducing penicllium oxalicum BZH-2002 with obvious disease-resistant effect. The production method of the pectinase biocontrol inducer comprises five steps of strain activation and storage, seed preparation, solid state fermentation, post-processing technology and determination of pectinase activity; and according to the production method, the apple waste residues are used as the main carbon sources of a fermentation medium, and an inorganic ammonium sulfate is used as nitrogen source. The technical scheme of the invention provides a new way for the resource utilization of the apple waste residues; and the produced pectinase biocontrol inducer can be applied to fields and has better prevention and cure effect on multiple plant diseases.

The invention belongs to the technical field of biology, and particularly relates to a preparation method for biotinylation luciferase. The method comprises the following steps that 1, luciferase is dissolved by using a PBS buffer solution; 2, biotin is activated; 3, the activated biotin is dissolved in mixed solvent, the luciferase is added, and stirring is performed; 4, dialysis is performed on a mixed solution by using the PBS buffer solution; 5, the protein content is measured by using the 280 nm absorbancy; 6, a protective agent is added, and cold preservation is performed. According to the preparation method for the biotinylation luciferase, the obtained biotinylation luciferase is high in biological activity.

The invention, which belongs to the technical field of biology, discloses an enzyme for preparing an antidepressant drug duloxetine intermediate, a biological catalysis method and application of the enzyme. 3-dimethylamino-1-(2-thienyl)-1-acetone hydrochloride can be transformed into S-3-dimethylamino-1-(2-thienyl)-1-propanol and high dehydrogenase activity is realized; and the enzyme has one or more mutations of K49R, A68T, E101D, F147E, T152A, S169V and A235S. The enzyme has obvious high specific enzyme activity that is improved by 2-10 times than that of wild type ketoreductase; and the enzyme can be used for biologically catalyzing transformation of 3-dimethylamino-1-(2-thienyl)-1-acetone hydrochloride to S-3-dimethylamino-1-(2-thienyl)-1-propanol; the reaction conditions are mild; equipment requirements are low; no high temperature or cooling in the production process is needed; energy consumption is low; purification is convenient; and the green and environment-friendly preparation process is realized.

The invention discloses a bactericide for treating downy mildew, and relates to the chemical engineering technical field. The bactericide for treating downy mildew comprises the components by the mass percentage: 40-60% of dimethomorph, 10-20% of eugenol, 15-25% of polyoxin, 1-5% of a 'Guidanmei' biological auxiliary agent, and 1-5% of a biological antibacterial factor. According to the bactericide, through optimizing the proportion of all the components, the prepared bactericide for treating downy mildew is low in bactericide production cost, good in sterilization effect, easy to dissolve, strong in adhesion force and friendly to the environment.

The invention discloses a composite organophosphorus degrading enzyme biological decontamination agent for emergency rescue and decontamination operation at chemical disaster accident environmental scene. The decontamination agent mainly consists of organophosphorus hydrolase, organophosphorus anhydride hydrolase, diisopropyl fluorine phosphatase, organochlorine degrading enzyme and trehalose, wherein all the components reach the following contents in percentages: 80-90% of organophosphorus hydrolase, 3-5% of organophosphorus anhydride hydrolase, 2.5-3% of diisopropyl fluorine phosphatase, 5-10% of organochlorine degrading enzyme and 0.5-1% of trehalose. The decontamination agent has the following advantages: compared with traditional chemical, physical and mechanical decontamination modes, the composite organophosphorus degrading enzyme biological decontamination agent has such advantages as no poison, no corrosion, high catalysis efficiency, low use level and wide application range, can be used for decontamination and disinfection for staff, clothes, equipment, soil and water sources polluted by organophosphorus, organochlorine and sulfides, in particular, is suitable for emergency rescue and decontamination in pesticide leakage accident scenes, and cannot cause secondary environmental pollution.

A solid enzyme type time-temperature indicator is composed of two parts, namely a laccase PVA gel slice and a guaiacol PVA gel slice, when in use, the two parts fit to form the solid enzyme type time-temperature indicator, wherein the laccase PVA gel slice is a PVA gel slice embedded with laccase, and the guaiacol PVA gel slice is a PVA gel slice embedded with guaiacol. The solid enzyme type time-temperature indicator provided by the invention has the beneficial effects that the solid enzyme type time-temperature indicator can be used for indication of freshness of food with the activation energy of 9.5-68.8kJ/mol, a hidden danger that leakage of a liquid-state TTI package can be easily caused can be completely eradicated, and an indicator does not need to be additionally added like lipase, amylase and urease; the method provided by the invention avoids the situation that using effect of TTI is influenced as diffusion of an enzyme and substrate is hindered by embedding and biological activity of the enzyme is reduced, and the PVA gel slices are used as a carrier, so that a system is uniformly mixed, color distributioh is also relatively uniform, lump and flocculent color blocks cannot be formed, and the solid enzyme type time-temperature indicator is easy to make, stable in effect, low in cost and easy to popularize.

The invention belongs to the technical field of biological detection and particularly relates to an apoptosis kit applied to circulating tumor cell detection and a detection method thereof. The kit comprises a kit body, 200 mu L of 1% Triton X-100, 100 mu L of streptavidin labeled with TRITC, 200 mu L of deoxyribonuclease I, 200 mu L of deoxyribonuclease i buffer solution, 1 mL of equilibrium buffer solution, 80 mu L of TdT enzyme, 20 mu L of biotin-11-dUTP, 1.0 mL of labeling buffer solution and 50 mu L of bacterial lipopolysaccharide buffer solution. The kit provided by the invention can beused for detecting apoptotic cells only through one-step staining, and meanwhile, and the kit is specifically applied to detection of apoptotic circulating tumor cells, and is good in sensitivity, specificity and detection effect.

The invention provides a Tb-GMP/CeO2 composite material and a preparation method thereof and a method for detecting ziram, and belongs to the technical field of pesticide detection. The composite material is of a spherical structure, wherein the size is 10-50 nm, and the physical property parameters of X-ray photoelectron spectroscopy are terbium (3d3/2) 1277.1 eV and the like. The Tb-GMP/CeO2 composite material provided by the invention is combined with tyrosinase to form an enzyme biosensor, so that ziram in a sample can be detected.

The invention discloses a method for treating black odorous anaerobic sludge by utilizing microorganisms. The method comprises the following steps: S1, crushing rice chaff and straws into powder, andcarrying out uniform mixing and stirring to obtain a raw material mixture; S2, soaking the raw material mixture with a water aqua of low-temperature domesticated microbes; and S3, subjecting the soaked raw material mixture and sludge to be purified to stirring and mixing, then adding a catalytic enzyme biological composting agent, carrying out full mixing, exposing the obtained mixture to air, andconducting standing to purify the sludge so as to obtain purified soil. According to the method, pollutants in an organic river (sludge) are rapidly decomposed by utilizing the microbe, and the contents of organic matters such as ammonia, nitrogen, phosphorus and potassium in the sludge are rapidly reduced through decomposition, absorption and transfer, so the organic matters are volatilized in agaseous form, and soil components mainly containing inorganic matters are formed; and therefore, optimal treatment effect is achieved, no secondary pollution is generated, and the aims of treating both symptoms and root causes are achieved. Moreover, on-site direct catalytic decomposition is realized, so long-distance transportation is avoided, and the method has the advantages of safety, high efficiency, low cost, reduced investment, simplicity in operation and the like.