14 results about "Enzybiotics" patented technology

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

An enzyme obtained from the saliva of waxworms (Galleria mellonella larvae) is disclosed that has the unexpected ability to oxidize and depolymerize untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH, and short incubation times. The present invention also relates to a method for biodegrading polyolefin-derived polymers using this enzyme.

The invention discloses an enzyme biosensor for rapid detection of organophosphorus pesticide dimethoate as well as preparation and application of the enzyme biosensor, and relates to the field of electrochemical sensors. The method comprises the following steps: preparing GR / GCE, preparing AuNPs / GR / GCE, and preparing AChE / AuNPs / GR / GCE. Graphene and gold nanoparticles are compounded to form AuNPs / GR / GCE, the electrochemical impedance of the glassy carbon electrode is remarkably reduced, the effective specific surface area of the glassy carbon electrode is increased, the electron transfer rate of the surface of the glassy carbon electrode is increased, rich binding sites are provided for immobilization of enzyme molecules, the stability of enzyme is improved, and degradation or inactivation of the enzyme in the using process is reduced; acetylcholine esterase is used as a recognition probe, AChE / AuNPs / GR / GCE is constructed on AuNPs / GR / GCE in a self-assembly mode, dimethoate can prevent acetyl thiocholine iodide from being hydrolyzed to generate thiocholine, electrochemical response signals are reduced, and the dimethoate has good electrochemical response signals, so that the organophosphorus pesticide dimethoate residue is rapidly, accurately and safely screened on site.

The application discloses an aldolase bioelement screening method and related application of a series of aldolases in catalyzing a condensation reaction of formaldehyde and pyruvic acid to synthesize 4-hydroxy-2-ketobutyric acid. The aldolase provided by the application is a protein shown in SEQ ID NO. 1-NO. 10, or a functionally identical protein obtained through substitution and / or deletion / addition of one or more amino acid residues. The aldolase has the following characteristics: 1) belonging to the HpcH functional domain family protein; 2) the sequence length is 240-320 amino acids; 3) the existing database function annotation is 4-hydroxy-2-oxovalerate aldolase (can catalyze the condensation reaction of acetaldehyde and pyruvic acid); the aldolase can take formaldehyde and pyruvic acid as substrates, and can carry out enzyme catalysis reaction in appropriate conditions and medium, and can efficiently biosynthesize 4-hydroxy-2-ketobutyric acid, and has the remarkable characteristics of mild reaction condition, good stereoselectivity and no pollution.

The invention belongs to the technical field of biological catalysis, and particularly relates to an anchor peptide mediated fusion protein and an immobilized enzyme biofilm reactor, the fusion protein comprises a functional enzyme protein and an anchor peptide mutant; the anchoring peptide mutant is obtained by mutating proline at the fifth position of an amino acid sequence as shown in SEQ ID No.2 into valine; the functional enzyme protein is nicotinamide ribokinase or polyphosphate kinase. The functional enzyme protein and the anchoring peptide mutant are connected by using the connecting peptide, and then the obtained fusion protein is anchored on the polypropylene hollow fiber membrane wire material, so that the obtained fusion protein has good anchoring effect and catalytic activity, the batch reaction yield stability can be obviously improved, and the preparation method is suitable for industrial production. An immobilized enzyme bio-membrane reactor is designed, and nicotinamide mononucleotide can be synthesized through biological catalysis.

Enzymes for biodegrading polyolefin-derived polymers and methods of using same. The present invention relates to enzymes derived from the saliva of waxworms (Galleria mellonella larvae) that have the unexpected ability to oxidize and depolymerize untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH, and short incubation times. The present invention also relates to methods for biodegrading polyolefin-derived polymers using the enzymes.

The present application belongs to the technical field of sewage treatment risk early warning, and discloses a method for predicting sludge bulking by integrating sludge volume and toxicity effect in a sewage treatment process, which comprises the following steps: S1. calculating RIS according to the integrated sludge biological effect index and physicochemical index; the RIS is the product of the sludge functional enzyme biological index (the ratio of the maximum effect concentration to the half effect concentration) and the physicochemical index (the ratio of the measured SVI value to the limited SVI value of sludge bulging); S2. defining the safe range of sludge bulging under the RIS according to the risk event probability statistics, and defining the success event and the failure event as RIS<2 and RIS>2 respectively; MOS=L * N max ‑(LN max ‑Z risk S * )=Z risk S * (5) wherein: S * : the standard deviation of the process index of the sewage plant; Z risk : the standard normal quantile of any specified acceptable risk level, and the value is obtained by consulting the normal distribution table; the evaluation result of the present application is high in accuracy, and to a certain extent, prevents the limitations brought by sludge bulging faced by the sewage treatment process system.

The invention discloses a PROTAC compound for degrading TRIM15 protein. The PROTAC compound has a structure as shown in a formula (I). The molecule can effectively inhibit the RIG-1 signal channel, and has application prospects in preparation of anti-inflammatory or autoimmune disease drugs. Meanwhile, the invention further provides a chemical probe molecule with the structure shown in the formula (III). One end of the molecule is combined with TRIM15, and the other end of the molecule can be combined with tag protein (such as Halog-tag) or other target protein, so that TRIM15 is recruited as E3 ubiquitin ligase to carry out ubiquitination modification on the tag protein (or the target protein). The molecule can be used as an innovative research tool and is used for exploring the biological function of the E3 ligase of TRIM15, the substrate specificity and non-K48 type ubiquitination modification.

The application discloses a kind of yeast black tea products and process method for preparing by double biological enzyme fermentation, which includes the process steps of picking and removing impurities of fresh leaves, light heat wilting, light heat steam biological fermentation, enzyme oxygenation kneading, deagglomeration double enzyme biological fermentation, hot air aroma extraction initial baking and vacuum freeze drying etc.The yeast black tea with higher fermentation enzymolysis power can be prepared by process optimization, which has strip shape, red leaves, sweet aroma, the activity of tea polyphenol oxidase (ppo) is kept at 1.5-2 U / G, and can drive other plant leaves to carry out mixed fermentation enzymolysis reaction, to make high-quality composite black tea, which has wide application prospect in the field of fermentation composite black tea.

Enzymes from the wax worm (Galleria mellonella larvae) saliva are disclosed which have the unexpected capacity of oxidizing and depolymerizing untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH and short incubation times. The invention also refers to a method for biodegrading a polyolefin-derived polymer wherein this enzyme is used.

The application provides a paper-based enzyme biosensor for hypoxanthine detection and a preparation method thereof, and comprises paper-based chip manufacturing, enzyme solution, silicon precursor solution and mineralization system solution required by a synthesis xanthine oxidase silicon enzyme method, color developing system configuration, paper-based chip modification and packaging. Through the technical scheme of the application, xanthine oxidase (XOD) is combined with color developing agent nitro blue tetrazolium chloride (NBT), one-step color development is realized, the operation process is significantly simplified, and the stability of the color developing system is improved. The enzyme biosensor constructed based on the biomimetic mineralization immobilization system can maintain high enzyme activity after being stored at room temperature for 21 days, exhibits excellent room temperature storage stability, is more convenient for practical application and storage and transportation, and greatly reduces the use amount of enzymes, thereby saving costs while ensuring high sensitivity and reliable quantitative detection performance.

The invention relates to the field of biotechnology engineering, in particular to a method for synthesizing 3-hydroxy-3-methylbutyric acid through biological catalysis. A recombinant escherichia coli strain is designed through a genetic engineering method, three gene segments of L-AAD, 4-HPPD and Linker are integrated onto a plasmid vector by utilizing a genetic engineering technology, the recombinant escherichia coli is obtained by conversion, and the recombinant escherichia coli strain for expressing L-amino acid deaminase and 4-hydroxyphenylpyruvate dioxygenase which are connected by utilizing Linker is constructed. Then leucine is used as a substrate, and the 3-hydroxy-3-methylbutyric acid is synthesized through biological catalysis of the two enzymes connected by Linker. According to the method disclosed by the invention, a direct fermentation method instead of catalysis after enzyme extraction is adopted, so that the step of enzyme extraction is not needed, a large amount of culture solution is not wasted, and the purposes of reducing operation steps, reducing side reaction processes and reducing raw material waste can be achieved.

The invention discloses a BCCP fusion targeting-based chlamydomonas reinhardtii metabolic enzyme protein nuclear localization method, which comprises the following steps: carrying out fusion expression on acetyl coenzyme A carboxylase (ACC) biotin carboxyl carrier protein (BCCP) subunit and target protein, and realizing specific enrichment of fusion protein on the periphery of a protein nucleus by utilizing the natural protein nucleus targeting characteristic of BCCP. The invention provides a novel method for constructing a chlamydomonas reinhardtii engineering strain capable of efficiently utilizing inorganic carbon; the method has important application value in the fields of microalgae carbon capture, biofuel production, high-added-value compound synthesis and the like.

The invention discloses a method for preparing amine through biological catalysis, which comprises the following steps: providing raw materials including coenzyme, biological catalysis enzyme, aniline compounds and chain ketone; and performing biological catalysis, namely mixing the raw materials, and incubating, so that the biological catalysis enzyme and the coenzyme catalyze the aniline compound and the chain ketone to perform reductive amination reaction in a reaction system to obtain amine. The method solves the problem that aniline compounds and chain ketones are not easy to carry out reductive amination.