28 results about "Enzybiotics" patented technology

The invention discloses a preparation method and application of an iron-based covalent organic framework enzyme-loaded biological cascade catalytic material, and belongs to the field of materials. An iron-based covalent organic framework material is prepared by adopting a one-pot method, and glucose oxidase is further loaded, so that the biological cascade catalytic material for glucose detection is prepared. The biological cascade catalytic material obtained by the preparation method disclosed by the invention combines the advantages of nano enzyme and natural enzyme, and has excellent catalytic efficiency and long catalytic life; the Fe-COFs prepared by the method disclosed by the invention is strong in peroxidase-like activity and high in enzyme loading capacity, can adapt to a cascade system loaded by various enzymes, and widens the application prospect of constructing a biological cascade catalytic system based on COFs loaded natural enzymes.

An enzymatic bioremediation reactor device for the accelerated composting of lignocellulose material, consisting of a sealed, insulated container, a mechanical pretreatment unit and an automated laccase enzyme supply system.

An enzyme obtained from the saliva of waxworms (Galleria mellonella larvae) is disclosed that has the unexpected ability to oxidize and depolymerize untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH, and short incubation times. The present invention also relates to a method for biodegrading polyolefin-derived polymers using this enzyme.

The invention discloses a biological agent for efficiently degrading mycotoxin, a method and application. Belongs to the technical field of enzyme biological preparations. The novel laccase is obtained from a Ganoderma lucidum culture solution and is named as GL-Lac, the amino acid sequence of the novel laccase is shown as SEQ ID NO.1, the novel laccase can effectively degrade AFB1, ZEN, DON, T-2 and other mycotoxins, non-phenolic toxin-AFB1 can be efficiently oxidized without additional mediators, in addition, by means of a composite induction strategy, the degradation rates of AFB1, ZEN, DON and T-2 toxins are remarkably increased and reach 80% or above, 90% or above, 78% or above and 75% or above respectively, and the novel laccase is suitable for industrial production. And a technical support is provided for degradation of mycotoxin.

The invention discloses an enzyme biosensor for rapid detection of organophosphorus pesticide dimethoate as well as preparation and application of the enzyme biosensor, and relates to the field of electrochemical sensors. The method comprises the following steps: preparing GR / GCE, preparing AuNPs / GR / GCE, and preparing AChE / AuNPs / GR / GCE. Graphene and gold nanoparticles are compounded to form AuNPs / GR / GCE, the electrochemical impedance of the glassy carbon electrode is remarkably reduced, the effective specific surface area of the glassy carbon electrode is increased, the electron transfer rate of the surface of the glassy carbon electrode is increased, rich binding sites are provided for immobilization of enzyme molecules, the stability of enzyme is improved, and degradation or inactivation of the enzyme in the using process is reduced; acetylcholine esterase is used as a recognition probe, AChE / AuNPs / GR / GCE is constructed on AuNPs / GR / GCE in a self-assembly mode, dimethoate can prevent acetyl thiocholine iodide from being hydrolyzed to generate thiocholine, electrochemical response signals are reduced, and the dimethoate has good electrochemical response signals, so that the organophosphorus pesticide dimethoate residue is rapidly, accurately and safely screened on site.

The application discloses an aldolase bioelement screening method and related application of a series of aldolases in catalyzing a condensation reaction of formaldehyde and pyruvic acid to synthesize 4-hydroxy-2-ketobutyric acid. The aldolase provided by the application is a protein shown in SEQ ID NO. 1-NO. 10, or a functionally identical protein obtained through substitution and / or deletion / addition of one or more amino acid residues. The aldolase has the following characteristics: 1) belonging to the HpcH functional domain family protein; 2) the sequence length is 240-320 amino acids; 3) the existing database function annotation is 4-hydroxy-2-oxovalerate aldolase (can catalyze the condensation reaction of acetaldehyde and pyruvic acid); the aldolase can take formaldehyde and pyruvic acid as substrates, and can carry out enzyme catalysis reaction in appropriate conditions and medium, and can efficiently biosynthesize 4-hydroxy-2-ketobutyric acid, and has the remarkable characteristics of mild reaction condition, good stereoselectivity and no pollution.

The present disclosure relates to biocide compositions compatible with enzyme biosensors and methods of use thereof. More particularly, the present disclosure relates to biocide compositions compatible with enzymes used to measure creatine and creatinine levels.

The invention belongs to the technical field of biological catalysis, and particularly relates to an anchor peptide mediated fusion protein and an immobilized enzyme biofilm reactor, the fusion protein comprises a functional enzyme protein and an anchor peptide mutant; the anchoring peptide mutant is obtained by mutating proline at the fifth position of an amino acid sequence as shown in SEQ ID No.2 into valine; the functional enzyme protein is nicotinamide ribokinase or polyphosphate kinase. The functional enzyme protein and the anchoring peptide mutant are connected by using the connecting peptide, and then the obtained fusion protein is anchored on the polypropylene hollow fiber membrane wire material, so that the obtained fusion protein has good anchoring effect and catalytic activity, the batch reaction yield stability can be obviously improved, and the preparation method is suitable for industrial production. An immobilized enzyme bio-membrane reactor is designed, and nicotinamide mononucleotide can be synthesized through biological catalysis.

The invention refers to enzymes from the wax worm (Galleria mellonella larvae) saliva which has the unexpected capacity of oxidizing and depolymerizing untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH and short incubation times. The invention also refers to methods for biodegrading a polyolefin-derived polymer wherein this enzyme is used.

Enzymes for biodegrading polyolefin-derived polymers and methods of using same. The present invention relates to enzymes derived from the saliva of waxworms (Galleria mellonella larvae) that have the unexpected ability to oxidize and depolymerize untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH, and short incubation times. The present invention also relates to methods for biodegrading polyolefin-derived polymers using the enzymes.

The utility model relates to the field of biotransformation, in particular to a device for enzyme biotransformation, which comprises a transformation box, the transformation box is arranged on a placing frame, a sealing upper cover is arranged at the top of the transformation box, a motor is arranged in the middle of the sealing upper cover, a through hole is arranged on the upper surface of the sealing upper cover and positioned on one side of the motor, and an enzyme medicament needle cylinder is arranged at the through hole. A pair of mounting frames are arranged on a sealing upper cover of a conversion box, enzyme medicament needle cylinders can be effectively mounted on the mounting frames, the enzyme medicament needle cylinders can be mounted and dismounted conveniently and quickly only by rotating a limiting plate, a piston is pushed through cooperation of a servo electric cylinder and movement of a telescopic rod, and therefore quantitative enzyme extrusion can be achieved; therefore, the extending length of the telescopic rod can be accurately controlled, the enzyme extrusion amount can be accurately controlled, the method is more accurate, an operator can clearly know the enzyme extrusion amount, and waste is effectively reduced.

The present application belongs to the technical field of sewage treatment risk early warning, and discloses a method for predicting sludge bulking by integrating sludge volume and toxicity effect in a sewage treatment process, which comprises the following steps: S1. calculating RIS according to the integrated sludge biological effect index and physicochemical index; the RIS is the product of the sludge functional enzyme biological index (the ratio of the maximum effect concentration to the half effect concentration) and the physicochemical index (the ratio of the measured SVI value to the limited SVI value of sludge bulging); S2. defining the safe range of sludge bulging under the RIS according to the risk event probability statistics, and defining the success event and the failure event as RIS<2 and RIS>2 respectively; MOS=L * N max ‑(LN max ‑Z risk S * )=Z risk S * (5) wherein: S * : the standard deviation of the process index of the sewage plant; Z risk : the standard normal quantile of any specified acceptable risk level, and the value is obtained by consulting the normal distribution table; the evaluation result of the present application is high in accuracy, and to a certain extent, prevents the limitations brought by sludge bulging faced by the sewage treatment process system.

The invention discloses a PROTAC compound for degrading TRIM15 protein. The PROTAC compound has a structure as shown in a formula (I). The molecule can effectively inhibit the RIG-1 signal channel, and has application prospects in preparation of anti-inflammatory or autoimmune disease drugs. Meanwhile, the invention further provides a chemical probe molecule with the structure shown in the formula (III). One end of the molecule is combined with TRIM15, and the other end of the molecule can be combined with tag protein (such as Halog-tag) or other target protein, so that TRIM15 is recruited as E3 ubiquitin ligase to carry out ubiquitination modification on the tag protein (or the target protein). The molecule can be used as an innovative research tool and is used for exploring the biological function of the E3 ligase of TRIM15, the substrate specificity and non-K48 type ubiquitination modification.

The application discloses a kind of yeast black tea products and process method for preparing by double biological enzyme fermentation, which includes the process steps of picking and removing impurities of fresh leaves, light heat wilting, light heat steam biological fermentation, enzyme oxygenation kneading, deagglomeration double enzyme biological fermentation, hot air aroma extraction initial baking and vacuum freeze drying etc.The yeast black tea with higher fermentation enzymolysis power can be prepared by process optimization, which has strip shape, red leaves, sweet aroma, the activity of tea polyphenol oxidase (ppo) is kept at 1.5-2 U / G, and can drive other plant leaves to carry out mixed fermentation enzymolysis reaction, to make high-quality composite black tea, which has wide application prospect in the field of fermentation composite black tea.

Enzymes from the wax worm (Galleria mellonella larvae) saliva are disclosed which have the unexpected capacity of oxidizing and depolymerizing untreated polyolefin-derived polymers, such as polyethylene (PE), at room temperature (RT), neutral pH and short incubation times. The invention also refers to a method for biodegrading a polyolefin-derived polymer wherein this enzyme is used.

The invention discloses portable fruit and vegetable safety detection equipment which comprises a detection base, a plurality of detection insertion pipes with openings in the upper ends are arranged at the upper end of the detection base, enzyme biosensors are arranged at the bottoms in the detection insertion pipes, filter layers are arranged at the positions, corresponding to the enzyme biosensors, of the detection insertion pipes, and top shafts are upwards arranged on the filter layers; a plurality of liquid leakage holes are formed in the periphery, corresponding to the top shaft, of the filtering layer; the elution pipes are in one-to-one correspondence with the detection insertion pipes, one end of each elution pipe is open, the free end of each elution pipe is in threaded connection with a sealing cover, a liquid outlet hole is formed in the middle of the sealing cover in a penetrating mode, a limiting cover covering the corresponding liquid outlet hole is arranged in the sealing cover, and a plurality of through holes are formed in the side wall of the limiting cover; sealing blocking pieces used for blocking the liquid outlet holes are connected into the limiting covers in a sliding mode, and supporting springs are arranged between the sealing blocking pieces and the bottoms of the corresponding limiting covers. According to the invention, pesticide residue detection can be simultaneously carried out on a plurality of samples, the batch detection time is effectively shortened, and the working efficiency is improved.

Slickwater fracturing fluids comprising one or more enzymes, one or more organisms capable of generating the one or more enzymes, or any combination thereof, may increase hydrocarbon production from a subterranean formation. In an aspect, slickwater fracturing fluids may comprise: an aqueous fluid, the enzymes, the organisms, or any combination thereof; proppant particulates; a friction reducer; and optionally, one or more additives. In an aspect, methods may comprise introducing the slickwater fracturing fluid into a subterranean formation; and contacting the slickwater fracturing fluid with a matrix of the subterranean formation. In an embodiment, methods may comprise introducing the slickwater fracturing fluid to the subterranean formation at a pressure and / or a flow rate sufficient to create and / or enlarge one or more fractures in the subterranean formation, and to force the slickwater fracturing fluid into the one or more fractures in the subterranean formation.

The application provides a paper-based enzyme biosensor for hypoxanthine detection and a preparation method thereof, and comprises paper-based chip manufacturing, enzyme solution, silicon precursor solution and mineralization system solution required by a synthesis xanthine oxidase silicon enzyme method, color developing system configuration, paper-based chip modification and packaging. Through the technical scheme of the application, xanthine oxidase (XOD) is combined with color developing agent nitro blue tetrazolium chloride (NBT), one-step color development is realized, the operation process is significantly simplified, and the stability of the color developing system is improved. The enzyme biosensor constructed based on the biomimetic mineralization immobilization system can maintain high enzyme activity after being stored at room temperature for 21 days, exhibits excellent room temperature storage stability, is more convenient for practical application and storage and transportation, and greatly reduces the use amount of enzymes, thereby saving costs while ensuring high sensitivity and reliable quantitative detection performance.

The present invention discloses a controllable electric field-enhanced enzymatic degradation method and application for in-situ remediation of organic contaminated soil, belonging to the technical field of in-situ remediation of organic contaminated soil. The method comprises the following steps: setting electrode wells in the remediation area, the electrode wells being arranged in a rectangular shape to form a non-uniform symmetrical electric field; inserting electrodes into the electrode wells, fixing them, and connecting them to the corresponding output ends of a DC power supply; pumping an electrolyte containing immobilized enzymes into the anode wells and into the cathode wells; setting the potential gradient of the electric field for transporting the immobilized enzymes and starting the DC power supply; after completing the electrokinetic transport of the immobilized enzymes, adjusting the DC electric field potential gradient and starting electrokinetic enhanced degradation. The present invention achieves low-carbon, green in-situ remediation of organic contaminated soil based on enzyme bioelectrochemical degradation and microelectrode redox pathways.

The invention relates to the field of biotechnology engineering, in particular to a method for synthesizing 3-hydroxy-3-methylbutyric acid through biological catalysis. A recombinant escherichia coli strain is designed through a genetic engineering method, three gene segments of L-AAD, 4-HPPD and Linker are integrated onto a plasmid vector by utilizing a genetic engineering technology, the recombinant escherichia coli is obtained by conversion, and the recombinant escherichia coli strain for expressing L-amino acid deaminase and 4-hydroxyphenylpyruvate dioxygenase which are connected by utilizing Linker is constructed. Then leucine is used as a substrate, and the 3-hydroxy-3-methylbutyric acid is synthesized through biological catalysis of the two enzymes connected by Linker. According to the method disclosed by the invention, a direct fermentation method instead of catalysis after enzyme extraction is adopted, so that the step of enzyme extraction is not needed, a large amount of culture solution is not wasted, and the purposes of reducing operation steps, reducing side reaction processes and reducing raw material waste can be achieved.

The present invention discloses a wastewater treatment device based on an enzyme biocatalyst, which relates to the field of wastewater treatment devices and includes a base. The upper end surface of the base is provided with a first treatment barrel and a second treatment barrel. The first treatment barrel and the second treatment barrel are both fixedly connected to the base, and a mixing component is installed between the first treatment barrel and the second treatment barrel. The two sides of the mixing component respectively extend into the first treatment barrel and the second treatment barrel for mixing the wastewater in the first treatment barrel and the second treatment barrel with each other. In this application, by providing two treatment barrels on the base, it is convenient to carry out mixing reactions with the catalyst in the two treatment barrels respectively when treating wastewater. During the mixing reaction process, the mixing component can be used to exchange and mix the wastewater between the two treatment barrels, and the upper and lower layer wastewater can also be repeatedly mixed through the mixing component, so as to achieve more uniform mixing.

The invention discloses a BCCP fusion targeting-based chlamydomonas reinhardtii metabolic enzyme protein nuclear localization method, which comprises the following steps: carrying out fusion expression on acetyl coenzyme A carboxylase (ACC) biotin carboxyl carrier protein (BCCP) subunit and target protein, and realizing specific enrichment of fusion protein on the periphery of a protein nucleus by utilizing the natural protein nucleus targeting characteristic of BCCP. The invention provides a novel method for constructing a chlamydomonas reinhardtii engineering strain capable of efficiently utilizing inorganic carbon; the method has important application value in the fields of microalgae carbon capture, biofuel production, high-added-value compound synthesis and the like.

The present application proposes a method for detecting pesticide residues in food using an enzyme biosensor, which relates to the field of pesticide detection technology. The method comprises: cleaning and drying an electrode substrate and placing it in an incubator; performing multiple silver mirror reactions on the electrode substrate to form a silver reference electrode and a corresponding silver conductive tape; placing carbon slurry on the silver reference electrode and covering the electrode's protective layer with polyvinyl chloride insulating tape; cleaning the carrier surface, drying it, dripping a fixative, and then cutting it to obtain an enzyme sheet; fixing the enzyme sheet on a thin-layer base electrode to obtain an enzyme biosensor; dripping a pesticide solution onto the carbon slurry surface of the thin-layer base electrode, dispersing it onto the enzyme sheet, and removing the enzyme sheet after the reaction is completed; dripping a buffer solution onto the electrode, applying a voltage to obtain a response signal, and analyzing the composition of the pesticide solution. The enzyme biosensor of the present application detects pesticide residue oxidation signals with stable and regular results and can be widely used in the detection of pesticide residues in food.

The invention discloses a method for preparing amine through biological catalysis, which comprises the following steps: providing raw materials including coenzyme, biological catalysis enzyme, aniline compounds and chain ketone; and performing biological catalysis, namely mixing the raw materials, and incubating, so that the biological catalysis enzyme and the coenzyme catalyze the aniline compound and the chain ketone to perform reductive amination reaction in a reaction system to obtain amine. The method solves the problem that aniline compounds and chain ketones are not easy to carry out reductive amination.

The invention relates to the technical field of enzyme bioengineering, in particular to fungus-derived endogalacturonase as well as a preparation method and application thereof, a coding sequence of the endogalacturonase is derived from chondrotrichum solani, an amino acid sequence of the endogalacturonase Dseno-PGI is shown as SEQ ID NO. 1, and a coding sequence of the endogalacturonase Dseno-PGI is shown as SEQ ID NO. 2. By constructing the recombinant expression vector, the endo-PGI is efficiently expressed in a host cell, the obtained endo-PGI can improve the enzymolysis and digestion efficiency of caragana microphylla, and the utilization rate of lignocellulose biomass such as caragana microphylla is increased.

This application discloses a biological preparation, method and application for efficiently degrading mycotoxins. It belongs to the technical field of enzyme biological preparations. Ganoderma lucidum A new type of laccase was obtained from the culture medium, named GL-Lac, whose amino acid sequence is shown in SEQ ID NO.1. It can effectively degrade mycotoxins such as AFB1, ZEN, DON and T-2, and can efficiently oxidize non-phenolic toxin ‑AFB1 without the addition of external mediators. In addition, through a composite induction strategy, the degradation rates of AFB1, ZEN, DON and T-2 toxins were significantly improved, reaching ≥80%, ≥90%, ≥78% and ≥75%, respectively, providing technical support for the degradation of mycotoxins.

The invention relates to a mutant of high-activity cellobiose dehydrogenase and application of the mutant. The mutant is obtained by mutation of any one site of asparagine at the 288 site, asparagine at the 419 site, asparagine at the 456 site, asparagine at the 483 site, asparagine at the 627 site and asparagine at the 698 site of cellobiose dehydrogenase TfCDH. The cellobiose dehydrogenase TfCDH mutant is based on cellobiose dehydrogenase TfCDH, amino acids at the 288 site, the 419 site, the 456 site, the 483 site, the 627 site and the 698 site are selected for site-specific mutagenesis, and compared with wild type cellobiose dehydrogenase TfCDH, the cellobiose dehydrogenase TfCDH mutant has the advantage that the enzyme activity is obviously improved. Compared with the wild cellobiose dehydrogenase TfCDH, the cellobiose dehydrogenase TfCDH mutant has the advantages that the DCIP enzyme activity is improved by 70.05%, the Cytc specific enzyme activity is improved by 135.46%, the use of the cellobiose dehydrogenase is greatly promoted, and a brand new page is opened for the research and development of enzyme biosensors.

The present invention discloses a CYP71P2 enzyme, a biomaterial and applications thereof. The CYP71P2 enzyme is derived from a stem premature development mutant of Atractylodes lancea. The present invention clones and verifies for the first time the key role of the CYP71P2 enzyme in the biosynthesis pathway of sesquiterpenoid compounds. The enzyme and the biomaterial thereof can be used for the synthesis of sesquiterpenoid compounds, providing a new pathway for the synthesis of such compounds. The gene encoding the CYP71P2 enzyme is introduced into squalene synthase-knockout cerevisiae yeast to achieve efficient production of the sesquiterpenoid compound nerolidol, with a yield of up to 500 mg / L.