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Preparation method of enzyme biosensor for detecting formaldehyde gas

A biosensor and formaldehyde gas technology, applied in the field of sensors, can solve the problems of poor selectivity of formaldehyde gas sensors, susceptibility to interference from other gases, poor stability, etc., and achieve high gas collection efficiency, simple structure, and increased surface area.

Inactive Publication Date: 2012-05-02
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The above-mentioned formaldehyde gas sensor has the disadvantages of poor selectivity and being easily interfered by other gases
In addition, low sensitivity and poor stability are also problems that this type of sensor needs to solve

Method used

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  • Preparation method of enzyme biosensor for detecting formaldehyde gas
  • Preparation method of enzyme biosensor for detecting formaldehyde gas
  • Preparation method of enzyme biosensor for detecting formaldehyde gas

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0030] A polytetrafluoroethylene membrane (thickness 200-300 μm, porosity > 30%, pore size 1-5 μm) was attached to a carbon foam electrode (a disc with a thickness of 1-3 mm), and the edges were bound to a cylindrical plexiglass sleeve made working electrode.

[0031] The reaction chamber of the sensor is composed of two cylindrical plexiglass sleeves with different diameters. The small sleeve can be inserted into the large sleeve just right, and there is no liquid leakage between the contact surfaces of the two cylinder walls. The reaction chamber contains a three-electrode system. The polytetrafluoroethylene film in contact with the foam carbon sheet is bundled at the bottom of the large sleeve to form a working electrode, and then an enzyme-containing electrolyte solution is added into the large sleeve. Bind the dialysis membrane (MWCO 12-14kDa) on the bottom of the small sleeve, and then insert the small sleeve into the inside of the large sleeve until the dialysis membra...

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Abstract

The invention relates to an enzyme biosensor for detecting a formaldehyde gas. The enzyme biosensor which adopts the high catalytic activity and the high specificity of an enzyme and a microporous polytetrafluoroethylene membrane to acquire is used to detect and monitor the concentration of the trace formaldehyde gas. The formaldehyde gas which permeates the microporous membrane and foamed carbon to enter a sensor reaction chamber transmits electrons in the foamed carbon electrode through NAD<+> and benzoquinone under the catalysis of a formaldehyde dehydrogenase, so the concentration of the formaldehyde gas can be measured through the current detection. The sensor of the invention, which has the characteristics of simple structure, high sensitivity and difficult influence by other gases, is suitable for the medical examination and the continuous monitoring of indoor formaldehyde pollution.

Description

technical field [0001] The invention relates to a sensor, in particular to an enzyme biosensor specially used for detecting formaldehyde gas. Background technique [0002] Formaldehyde is an important organic raw material, but the health hazards of formaldehyde mainly manifest as irritation, sensitization, genotoxicity and reproductive toxicity. At present, researchers have proposed a variety of sensors for formaldehyde gas detection, some of which have entered the market. Such sensors can be divided into five major types: metal oxide type, surface acoustic wave type, chemiluminescent type, electrochemical type, and biological type. [0003] Using SnO 2 Nanocrystals (Zhang Jianrong, Gao Lian. Journal of Inorganic Materials 2005, 20, 465-469), SnCl 2 -InCl 3 Oxide film (Ji Zhenguo et al. Journal of Sensing Technology, 2004, 2, 277-279), La 2 o 3 doped nano In 2 o 3 Materials (Liu Ruzheng et al. Electronic Components and Materials 2006, 25, 15-17), Ag 2 O-doped nano-C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/413
Inventor 黄积涛邢达杰黄薇
Owner NANKAI UNIV
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