Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Apoptosis kit applied to circulating tumor cell detection and detection method thereof

A technology of tumor cells and kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of easy false positives and achieve good detection effect and specificity

Active Publication Date: 2020-08-25
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their own characteristics, circulating tumor cells will evolve a set of anti-apoptotic mechanisms to inhibit cell apoptosis, so that when the apoptotic cells are detected by traditional apoptosis kits, false positives are prone to occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apoptosis kit applied to circulating tumor cell detection and detection method thereof
  • Apoptosis kit applied to circulating tumor cell detection and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The specific detection method using the above kit is:

[0027] (1) The samples containing circulating tumor cells were treated with paclitaxel, washed twice with PBS for 5 minutes each time, and then added to 100 μL 1% Triton X-100 solution for 5 minutes;

[0028] (2) Add 20 μL DNase I and 60 μL DNase I buffer to the sample, treat at 37°C for 30 minutes, then immerse in PBS and rinse three times, 5 minutes each time;

[0029] (3) Then add 50 μL TdT enzyme reaction solution to the sample, put it in an incubator, and react in the dark at 37°C for 60 minutes. After the reaction, the sample is immersed in PBS and rinsed for 3 times, each time for 5 minutes, and avoid light during the period;

[0030] The specific configuration process of the TdT enzyme reaction solution is: add 1.0 μL biotin-11-dUTP and 4.0 μL TdT enzyme, add 45 μL equilibration buffer, and stir evenly;

[0031] (4) Add 50 μL of bacterial lipopolysaccharide buffer dropwise to each sample, let it stand for ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biological detection and particularly relates to an apoptosis kit applied to circulating tumor cell detection and a detection method thereof. The kit comprises a kit body, 200 mu L of 1% Triton X-100, 100 mu L of streptavidin labeled with TRITC, 200 mu L of deoxyribonuclease I, 200 mu L of deoxyribonuclease i buffer solution, 1 mL of equilibrium buffer solution, 80 mu L of TdT enzyme, 20 mu L of biotin-11-dUTP, 1.0 mL of labeling buffer solution and 50 mu L of bacterial lipopolysaccharide buffer solution. The kit provided by the invention can beused for detecting apoptotic cells only through one-step staining, and meanwhile, and the kit is specifically applied to detection of apoptotic circulating tumor cells, and is good in sensitivity, specificity and detection effect.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to an apoptosis kit for detecting circulating tumor cells and a detection method thereof. Background technique [0002] Circulating tumor cells (CTCs) are tumor cells shed from solid tumors and enter the peripheral blood circulation. Since their discovery in 1989, a variety of methods have been used to detect circulating tumor cells in peripheral blood. Recent studies have shown that its detection has important clinical significance for evaluating the prognosis of tumor patients, especially advanced tumor patients, and selecting appropriate individualized treatment. Because CTC detection has the characteristics of minimally invasive and real-time detection, it is called the "liquid biopsy" of tumors. [0003] Apoptosis kit is a kit used to detect phosphatidylserine that appears on the cell membrane surface during apoptosis. In the early stage of cell apopto...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64G01N1/28G01N33/50
CPCG01N21/6486G01N1/28G01N33/5091G01N33/5005G01N2510/00
Inventor 李胜李浩马莹李冰崔凯王振丹
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com