Tb-GMP/CeO2 composite material and preparation method thereof and method for detecting ziram
A composite material, thiram technology, applied in the field of pesticide detection
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[0079] The present invention also provides the Tb-GMP / CeO described in the technical scheme 2 The preparation method of composite material, comprises the following steps:
[0080] 1) Mix 5′-guanylate disodium solution with CeO 2 After the solution is mixed, stir for the first time to obtain the first agitator; the time for the first agitation is 20min;
[0081] 2) mixing the first stirrer obtained in the step 1) with the terbium chloride solution and stirring for the second time to obtain the second stirrer; the second stirring time is 30min;
[0082] 3) centrifuge the second agitator obtained in step 2), and the precipitate obtained is Tb-GMP / CeO 2 composite material.
[0083] The present invention combines 5'-guanylate disodium solution with CeO 2 After the solutions are mixed, the first stirring is carried out to obtain the first stirring product; the time of the first stirring is 20 minutes.
[0084] In the present invention, the 5'-guanylate disodium solution and CeO...
Embodiment 1
[0130] Tb-GMP / CeO 2 Preparation of composite materials:
[0131] Synthesis of Tb-GMP / CeO by two-step method 2 . First, nano-CeO was synthesized by precipitation method 2 , the specific synthesis steps are as follows: weigh 2.52g of cerium nitrate hexahydrate in a 200mL beaker, and dissolve it in 100mL of water-ethylene glycol mixture (volume ratio: 1:1). After the solution was heated to 60°C, 20 mL of ammonia water was quickly added and stirred vigorously for 3 h. After the mixture was cooled, it was centrifuged at 12000rpm for 10min to obtain yellow nano-CeO 2 precipitation. Nano-CeO was washed several times with ethanol and ultrapure water 2 until the washing solution is neutral. The washed nano-CeO 2 Mix with 100mL sodium citrate solution (concentration: 30g / L), after ultrasonication for 30min, continue to add ethanol until no precipitate is formed. The supernatant was discarded after the mixture was centrifuged at 12000 rpm for 10 min, and the precipitate was wash...
Embodiment 2
[0136] The Tb-GMP / CeO that embodiment 1 prepares 2 Composites testing ziram
[0137] First, a tyrosinase solution with a concentration of 175 U / mL was prepared using Tris-HCl buffer (concentration: 10 mM, pH=7.4) as a solvent. A series of concentrations (10 μg / L, 50 μg / L, 100 μg / L, 250 μg / L, 400 μg / L, 500 μg / L) of ziram solutions were prepared in acetonitrile for the quantitative detection of ziram by establishing a standard curve.
[0138] Take 5 μL of tyrosinase solution in a 2 mL plastic centrifuge tube, then add 5 μL of ziram solution to the centrifuge tube, mix the two thoroughly, and let stand at 37°C for 5 minutes. Afterwards, 50 μL of 2.5 mM tyrosine solution and 40 μL of 10 mM Tris-HCl buffer (pH=7.4) were added to the centrifuge tube, mixed evenly and left to react at 37° C. for 30 min. After the reaction is complete, add 100 μL of Tb-GMP / CeO to the centrifuge tube 2 aqueous solution (2 mg / mL concentration), 75 μL TMB (3,3’,5,5’-tetramethylbenzidine) solution, and...
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