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A tb-gmp/ceo 2 Composite material and its preparation method and method for detecting zirdine

A composite material, the technology of fumei zinc, applied in the field of pesticide detection

Active Publication Date: 2022-04-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no information on how to use nano-CeO in the prior art 2 to detect the report of siram

Method used

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  • A tb-gmp/ceo <sub>2</sub> Composite material and its preparation method and method for detecting zirdine
  • A tb-gmp/ceo <sub>2</sub> Composite material and its preparation method and method for detecting zirdine
  • A tb-gmp/ceo <sub>2</sub> Composite material and its preparation method and method for detecting zirdine

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preparation example Construction

[0079] The present invention also provides the Tb-GMP / CeO described in the technical scheme 2 The preparation method of composite material, comprises the following steps:

[0080] 1) Mix 5'-guanylate disodium solution with CeO 2 After the solution is mixed, stir for the first time to obtain the first agitator; the time for the first agitation is 20min;

[0081] 2) mixing the first stirred product obtained in the step 1) with the terbium chloride solution and then stirring for the second time to obtain the second stirred product; the time of the second stirred is 30min;

[0082] 3) centrifuge the second agitator obtained in step 2), and the precipitate obtained is Tb-GMP / CeO 2 composite material.

[0083] The present invention combines 5'-guanylate disodium solution with CeO 2 After the solutions are mixed, the first stirring is carried out to obtain the first stirring product; the time of the first stirring is 20 minutes.

[0084] In the present invention, the 5'-guanylat...

Embodiment 1

[0130] Tb-GMP / CeO 2 Preparation of composite materials:

[0131] Synthesis of Tb-GMP / CeO by two-step method 2 . First, nano-CeO was synthesized by precipitation method 2 , the specific synthesis steps are as follows: weigh 2.52g of cerium nitrate hexahydrate in a 200mL beaker, and dissolve it in 100mL of water-ethylene glycol mixture (volume ratio: 1:1). After the solution was heated to 60°C, 20 mL of ammonia water was quickly added and stirred vigorously for 3 h. After the mixture was cooled, it was centrifuged at 12000rpm for 10min to obtain yellow nano-CeO 2 precipitation. Nano-CeO was washed several times with ethanol and ultrapure water 2 until the washing solution is neutral. The washed nano-CeO 2 Mix with 100mL sodium citrate solution (concentration: 30g / L), after ultrasonication for 30min, continue to add ethanol until no precipitate is formed. The supernatant was discarded after the mixture was centrifuged at 12000 rpm for 10 min, and the precipitate was wash...

Embodiment 2

[0137] The Tb-GMP / CeO that embodiment 1 prepares 2 Composites testing ziram

[0138] First, a tyrosinase solution with a concentration of 175 U / mL was prepared using Tris-HCl buffer (concentration: 10 mM, pH=7.4) as a solvent. A series of concentrations (10 μg / L, 50 μg / L, 100 μg / L, 250 μg / L, 400 μg / L, 500 μg / L) of ziram solutions were prepared in acetonitrile for the quantitative detection of ziram by establishing a standard curve.

[0139] Take 5 μL of tyrosinase solution in a 2 mL plastic centrifuge tube, then add 5 μL of ziram solution to the centrifuge tube, mix the two thoroughly, and let stand at 37°C for 5 minutes. Afterwards, 50 μL of 2.5 mM tyrosine solution and 40 μL of 10 mM Tris-HCl buffer (pH=7.4) were added to the centrifuge tube, mixed evenly and left to react at 37° C. for 30 min. After the reaction is complete, add 100 μL of Tb-GMP / CeO to the centrifuge tube 2 aqueous solution (2 mg / mL concentration), 75 μL TMB (3,3’,5,5’-tetramethylbenzidine) solution, and...

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Abstract

The invention provides a Tb-GMP / CeO 2 The composite material, its preparation method and the method for detecting zirdine belong to the technical field of pesticide detection. The composite material has a spherical structure with a size of 10-50nm. The physical parameters of the X-ray photoelectron spectrum are: terbium (3d 3 / 2 ) 1277.1eV etc. Adopt Tb-GMP / CeO provided by the present invention 2 The composite material is combined with tyrosinase to form an enzyme biosensor, which can detect ziram in the sample.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, in particular to a Tb-GMP / CeO 2 Composite material and its preparation method and detection method of zirdine. Background technique [0002] Zirbane is a widely used dithiocarbamate fungicide for the prevention of fungal diseases in seeds and crops. At present, the standard detection method for ziram residues in agricultural products in my country is gas chromatography. In the gas chromatographic determination of ziram, it is necessary to heat zirdine under strong acidic conditions at a high temperature to convert it into carbon disulfide, and then determine the carbon disulfide by gas chromatography to realize the indirect determination of ziram content. This indirect method for the determination of ziram is cumbersome, time-consuming, and requires the use of strong acids, which does not conform to the principle of green chemistry to reduce the use of dangerous goods. In addition,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00C08K3/22G01N21/31G01N21/64G01N21/78
CPCC08G83/008C08K3/22G01N21/31G01N21/643G01N21/78C08K2003/2213C08K2201/011
Inventor 王鹏王冬伟周志强刘东晖
Owner CHINA AGRI UNIV
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