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Oral care agent with antibacterial effect and preparation method thereof

A technology of oral care agent and antibacterial effect, which is applied in the field of oral care agent with antibacterial effect and its preparation, and can solve problems such as not having antibacterial effect

Active Publication Date: 2020-10-30
SHANGHAI ZHONGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Montmorillonite has no direct antibacterial effect of antibacterial drugs

Method used

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  • Oral care agent with antibacterial effect and preparation method thereof
  • Oral care agent with antibacterial effect and preparation method thereof
  • Oral care agent with antibacterial effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, a kind of oral care agent with bacteriostasis to the inhibitory effect of oral cavity bacteria

[0037] In this experiment, the in vitro method was used to detect the effect of lysozyme oral care agent on the common oral bacteria Streptococcus mutans (Streptococcus mutans), Escherichia coli (Escherichia coli), Porphyromonas gingivalis (Porphyromonas gingivalis), Inhibition of Staphylococcus aureus and Prevotella intermedia. Due to the strong subjectivity of ordinary colony counting method, and the samples to be tested are often not completely dispersed into individual bacteria, the accuracy of plate colony counting results is often low, and a single colony may also come from 2 to 3 or more bacteria in the sample. Multiple cells, difficult to obtain accurate counts. Therefore, in this experiment, the Oxford cup method was used to directly observe the antibacterial effect of each experimental group. The Oxford cup method is a common method for antibacteria...

Embodiment 2

[0069] Embodiment two, WST-8 test each group antibacterial effect

[0070] Compared with the common colony counting method, the WST-8 method has the advantages of high sensitivity, reliable data, good repeatability, easy operation, etc., and the quantification of the antibacterial effect is more accurate.

[0071] 1. Experimental grouping

[0072] The experimental grouping is the same as in Example 1.

[0073] 2. Experimental process

[0074]The samples of each group cultured for 6h and 12h were tested by WST-8 kit. After each experimental group was cultured under corresponding bacterial culture conditions for a predetermined period of time, after standing still for 30 minutes, 3 samples of supernatant of each group were taken, each sample 0.15mL, and added to a 96-well plate. Add 15 μL of WST-8 reagent to each well of the 96-well plate, mix well and incubate at 37°C for 1 hour, then measure the OD with a full-wavelength microplate reader 450nm . The antibacterial rate ca...

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Abstract

The invention relates to the field of prevention and treatment of oral diseases, in particular to an oral care agent with an antibacterial effect, which comprises lysozyme and montmorillonite. The invention relates to a lysozyme biological toothpaste, which comprises 0.4% of lysozyme, 1% of montmorillonite, an abrasive, a wetting agent, an adhesive, a surfactant, a preservative, a sweetening agent, a thickening agent, a foaming agent and the balance of deionized water. The lysozyme biological toothpaste has an antibacterial effect on oral pathogenic bacteria, is wide in antibacterial spectrumand high in antibacterial rate, can efficiently inhibit growth of the oral pathogenic bacteria in a short time, is relatively low in minimum antibacterial concentration, and does not generate drug-resistant strains of the oral pathogenic bacteria after being used for a long time; the prepared toothpaste can be prevented from being affected by denaturation of lysozyme by substances such as a foaming agent, a binder, a sweetening agent and a preservative, and the activity of biological lysozyme is prevented from being damaged or reduced, so that the product plays a role in inhibiting oral pathogenic bacteria, can effectively play a role in preventing caries and periodontitis, and has unique advantages and wide application prospects.

Description

technical field [0001] The invention relates to the field of prevention and treatment of oral diseases, in particular to an oral care agent with antibacterial effect and a preparation method thereof. Background technique [0002] Common oral diseases such as dental caries, periodontitis, pericoronitis, and oral ulcers are important public health problems worldwide, and have been proven to be related to oral bacterial infections. Oral bacteria usually exist in the form of plaque biofilm, which adheres to and around the tooth surface and continues to produce acid, leading to demineralization of tooth enamel and eventually caries, periodontal disease and gingivitis. Inhibiting the growth of oral bacteria / killing oral bacteria is the ultimate goal of preventing and treating oral diseases. Fluoride, antibiotics and chemical disinfectants are common drugs for the prevention and treatment of oral diseases, and have achieved good results. However, in recent years, researchers have...

Claims

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Application Information

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IPC IPC(8): A61K8/66A61K8/26A61Q11/00A61P31/04
CPCA61K8/66A61K8/26A61Q11/00A61P31/04
Inventor 金家骅朱晓萍丁礼琴曹无介
Owner SHANGHAI ZHONGHUA PHARMA
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