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Novel Pb2+ supersensitive detecting method based on photoelectrochemical sensing

A photoelectrochemical, ultra-sensitive technology, applied in the field of photoelectrochemical sensing of analytical chemistry, to achieve good selectivity, high sensitivity, and easy operation

Inactive Publication Date: 2013-11-20
TAIZHOU UNIV
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Problems solved by technology

However, there are few reports on the use of photoelectrochemical sensing technology for the determination of metal ions. So far, there is no photoelectrochemical sensing technology for the determination of Pb 2+ report

Method used

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  • Novel Pb2+ supersensitive detecting method based on photoelectrochemical sensing
  • Novel Pb2+ supersensitive detecting method based on photoelectrochemical sensing

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Embodiment 1

[0022] Add 50mL0.01M CdCl to a 100mL three-neck flask 2 solution and 250 μL thioglycolic acid, while stirring, nitrogen gas was passed into the solution for 30 min. During this time, the pH of the mixture was adjusted to 11 using 1 M NaOH solution. Then, add 5 mL of 0.1M Na 2 S solution was heated to reflux at 110° C. with nitrogen for 4 h. In this series of experiments, CdS quantum dots with S / Cd ratio of 1:1 can be obtained. Immerse the cleaned and dried ITO electrode in 2% diethylene glycol diacrylate solution for 10 minutes, take it out and wash it with water; then immerse it in the spare CdS quantum dot solution for 10 minutes, take it out and wash it with water; repeat this process for 3 Once, the desired multilayer film modified electrode was obtained; next, the CdS quantum dot modified electrode was immersed in 20 mg of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride at room temperature Salt and 10 mg N-hydroxysuccinimide in 1.0 mL distilled water for on...

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Abstract

The invention relates to construction of a photoelectrochemical sensor which is based on conformation transition of G-quadruplex DNAzyme, and belongs to the field of photoelectrochemical sensor technology of analytical chemistry, wherein the conformation transition is induced by Pb2+, and the photoelectrochemical sensor is capable of realizing supersensitive sensing of Pb2+ effectively. K+ is replace by Pb2+ to induce the conformation transition of G-quadruplex DNAzyme so as to decrease enzymatic activity, so that enzyme biological catalytic precipitation reaction on an ITO electrode which is modified by CdS quantum dots is influenced, and photoelectrochemical sensing detection of Pb2+ is realized. G-quadruplex DNAzyme is applied in a photoelectrochemical sensing system for detection of Pb2+ for the first time; the photoelectrochemical sensor possesses advantages of high selectivity and high ensitivity; and detection limit of Pb2+ reaches to 1.0*10<-8>M.

Description

technical field [0001] The invention belongs to the technical field of photoelectrochemical sensing of analytical chemistry, and relates to a Pb-based 2+ The induced conformational transition of G-quadruplex DNA enzyme leads to the decrease of enzyme activity and the construction of photoelectrochemical sensor based on the change of photocurrent in the system, which can effectively realize the Pb 2+ Ultra sensitive detection. Background technique [0002] The photoelectrochemical process refers to the photoelectric conversion process of photosensitive materials through electronic excitation and charge transfer under the action of light. As an independent integrated detection device, electrochemical biosensors have had an increasingly important impact on the biochemical and medical fields. With the rapid development of nanotechnology and material chemistry, a new generation of photoelectrochemical biosensors has been developed based on the combination of photoelectrochemica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 唐文渊韩得满李永敏贾文平
Owner TAIZHOU UNIV
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