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Desertorin B, and extraction method and application thereof

An extraction method and strain culture technology are applied in the field of desertorin B preparation to achieve good anti-tumor activity and good cytotoxic activity

Active Publication Date: 2018-10-12
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on Desertorin B and its activity in endophytic fungi in cat feces

Method used

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  • Desertorin B, and extraction method and application thereof
  • Desertorin B, and extraction method and application thereof
  • Desertorin B, and extraction method and application thereof

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Embodiment Construction

[0035] Embodiments of the present invention are described in further detail below:

[0036] A desertorin B compound, the chemical structural formula of the compound:

[0037]

[0038] The extraction method of this compound is as follows:

[0039] (1) Carry out strain culture to cat feces endophytic fungus Aspergillus tubingensis DS37 in strain culture medium, specifically cultivate at 28° C. for 7-10 days;

[0040] Wherein, the strain medium contains mass percent components: glucose 3%, sodium nitrate 0.3%, dipotassium hydrogen phosphate 0.1%, potassium chloride 0.05%, anhydrous magnesium sulfate 0.05%, ferrous sulfate 0.001%, and the balance is water, natural pH;

[0041] (2) Carry out fermentation culture to the bacterial classification obtained in step (1) in the fermentation medium, concrete 20-24 DEG C under culture 35-40 days, take fermented product (9.2kg) as raw material with petroleum ether / ethyl acetate / methanol leaching 8 times, merging extracts and filtering...

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Abstract

The invention discloses Desertorin B, and an extraction method and application thereof. The molecular formula of the Desertorin B is C25H20O8. The extraction method is as the follows: (1) carrying outstrain cultivation on decaisnea insignis endophytic fungi aspergillus tubingensis DS37, (2) carrying out fermentation cultivation on the strain obtained in the step (1) in a fermentation culture medium, then carrying out extraction with petroleum ether, ethyl acetate and methanol for a plurality of times, mixing the extracting solutions, carrying out filtering, recovering the mixed solvent, carrying out reduced pressure concentration on the filtrate to obtain a crude extract, and finally carrying out silica gel column chromatography to obtain the Desertorin B. The Desertorin B prepared by themethod has a good effect of inhibiting setosphaeria turcica, and can be used for the technologies of agricultural prevention and control and chemical prevention and control. In addition, the Desertorin B has strong activity for resisting MCF-7 and HepG2 cell lines, and can provide a possibility for the development of anti-tumor medicines. At the same time, the Desertorin B has certain antioxidantactivity.

Description

technical field [0001] The invention relates to the field of desertorin B preparation, in particular to a desertorin B and its extraction method and application. Background technique [0002] Decaisnea insignis (Griff.) Hook.f. & Thomson) is a plant of the genus Decaisnea insignis (Lardiyabalaeeae). Clumping deciduous erect shrubs, widely distributed in southwestern and central China. Akebia plant cat's feces is a traditional folk Chinese herbal medicine in my country. It tastes pungent, sweet, and flat in nature. The root and fruit are sweet and cool. So far, research on cat feces has mainly focused on its phytochemical components, while relatively little research has been done on its endophytic fungi. According to reports, the main types of secondary metabolites of endophytic fungi include: alkaloids, terpenes, steroids, organic acids and other compounds, all of which have certain biological activities. However, there is no report about Desertorin B and its activity in ...

Claims

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Application Information

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IPC IPC(8): C07D311/54A01N43/16A01P3/00A01P1/00A61P31/00A61P35/00A61P39/06C12P17/16C12R1/66
CPCA01N43/16A61P31/00A61P35/00A61P39/06C07D311/54C12P17/162
Inventor 杨秀芳王宁宁陈镝马养民雒佳欣陈璞庄瑜瑜侯艳艳
Owner SHAANXI UNIV OF SCI & TECH
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