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Method for preparing 20(R)-ginsenoside Rh1 by biotransformation of panax notoginseng saponins

A technology for the transformation of Panax notoginseng total saponins and Sanqi, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of difficult purification, long cycle, unstable fermentation products, etc., and achieve high conversion rate, Low cost, overcoming the effect of long cycle

Active Publication Date: 2020-04-10
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a kind of biotransformation of Panax notoginseng total saponins to prepare 20 ( R )-ginsenoside Rh 1 method, using Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 , is a green, efficient, and specific fermentation method for preparing high-purity rare ginsenosides, which overcomes the shortcomings of the existing fermentation process, such as too long cycle, unstable fermentation products, and difficult purification

Method used

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  • Method for preparing 20(R)-ginsenoside Rh1 by biotransformation of panax notoginseng saponins
  • Method for preparing 20(R)-ginsenoside Rh1 by biotransformation of panax notoginseng saponins

Examples

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Effect test

Embodiment 1

[0024] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:

[0025] (1) Use conventional PDB medium to activate and cultivate Aspergillus tubingensis:

[0026] ①PDB medium: Take 200g of fresh commercially available potatoes, cut them into square potato pieces with a side length of 1cm, add 1000mL of purified water, heat for 30-50min until boiling, filter with 4 layers of gauze, dilute the purified water to 1L, add 20g of glucose per liter , mixed evenly, dispensed and sterilized for later use; media sterilization conditions: 121°C, 30min;

[0027] ② Take Aspergillus tubingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized PDB medium in each bottle. The culture temperature: 26.5 ℃;...

Embodiment 2

[0033] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:

[0034] (1) Activate and cultivate Aspergillus tubingensis using conventional Martin's medium:

[0035] ① Preparation of Martin's medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5.0g, glucose 10.0g, add purified water to 1L, medium sterilization conditions: 121°C, 30min;

[0036] ② Take Aspergillus tabingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized Martin's medium in each bottle. The culture temperature is: 27.5°C; shaker culture 5d; speed 170r min -1 , to obtain the seed solution of the bacterial strain;

[0037] (2) Inoculate the seed solution of the activated bacteria in step (1) into sterile Martin's ...

Embodiment 3

[0042] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:

[0043] (1) Activate and cultivate Aspergillus tubingensis using conventional Martin's medium:

[0044] ① Preparation of Martin's medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5.0g, glucose 10.0g, add purified water to 1L, medium sterilization conditions: 121°C, 30min;

[0045] ② Take Aspergillus tabingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized Martin's medium in each bottle. The culture temperature is: 28°C; shaker culture 3d; speed 170r min -1 , to obtain the seed solution of the bacterial strain;

[0046] (2) Inoculate the seed solution of the activated bacteria in step (1) into sterile Martin's me...

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Abstract

The invention discloses a method for preparing 20(R)-ginsenoside Rh1 by biotransformation of panax notoginseng saponins. The 20(R)-ginsenoside Rh1 is prepared by biotransformation of panax notoginsengsaponins by adopting aspergillus tubingensis. The strain adopted by the method is convenient and easy to obtain, the method is simple to operate, the product conversion yield is high, and the methodcan be used for mass preparation of the 20(R)-ginsenoside Rh1. The method is simple, efficient, safe and green and is low in cost, and provides a new method for industrial production, food productionand new medicine preparation.

Description

technical field [0001] The invention relates to a preparation of 20( R )-ginsenoside Rh 1 The method uses special extracellular or intracellular enzymes produced by microorganisms as biocatalysts to selectively hydrolyze the glycosyl side chains of the high-content main saponins in Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 . Background technique [0002] Modern pharmacological studies have shown that triol type 20 ( R )-ginsenoside Rh 1 It has a wide range of biological activities, especially in the anti-tumor effect. It is effective against human melanoma cells (A375-S2), human uterine cancer cells (HeLa cells), human fetal glioma cells (T98G cells), human breast cancer cells Both MDA-MB-231 and MCF-7 cell activity have inhibitory effect; some studies have shown that 20( R )-ginsenoside Rh 1 It can induce the apoptosis of cervical cancer Hela cell line and leukemia K562 cell line, and its anticancer effect is obvious, such as inhibiting the migr...

Claims

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Application Information

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IPC IPC(8): C12P33/20C12N1/14C12R1/66
CPCC12P33/20C12N1/14
Inventor 杨晓艳李瑞婷崔秀明曲媛杨野刘迪秋王承潇
Owner KUNMING UNIV OF SCI & TECH
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