Method for preparing 20(R)-ginsenoside Rh1 by biotransformation of panax notoginseng saponins
A technology for the transformation of Panax notoginseng total saponins and Sanqi, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of difficult purification, long cycle, unstable fermentation products, etc., and achieve high conversion rate, Low cost, overcoming the effect of long cycle
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Embodiment 1
[0024] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:
[0025] (1) Use conventional PDB medium to activate and cultivate Aspergillus tubingensis:
[0026] ①PDB medium: Take 200g of fresh commercially available potatoes, cut them into square potato pieces with a side length of 1cm, add 1000mL of purified water, heat for 30-50min until boiling, filter with 4 layers of gauze, dilute the purified water to 1L, add 20g of glucose per liter , mixed evenly, dispensed and sterilized for later use; media sterilization conditions: 121°C, 30min;
[0027] ② Take Aspergillus tubingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized PDB medium in each bottle. The culture temperature: 26.5 ℃;...
Embodiment 2
[0033] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:
[0034] (1) Activate and cultivate Aspergillus tubingensis using conventional Martin's medium:
[0035] ① Preparation of Martin's medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5.0g, glucose 10.0g, add purified water to 1L, medium sterilization conditions: 121°C, 30min;
[0036] ② Take Aspergillus tabingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized Martin's medium in each bottle. The culture temperature is: 27.5°C; shaker culture 5d; speed 170r min -1 , to obtain the seed solution of the bacterial strain;
[0037] (2) Inoculate the seed solution of the activated bacteria in step (1) into sterile Martin's ...
Embodiment 3
[0042] Aspergillus tubingensis ( Aspergillus tubingensis ) Biotransformation of Panax notoginseng saponins to prepare 20 ( R )-ginsenoside Rh 1 method, proceed as follows:
[0043] (1) Activate and cultivate Aspergillus tubingensis using conventional Martin's medium:
[0044] ① Preparation of Martin's medium: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, peptone 5.0g, glucose 10.0g, add purified water to 1L, medium sterilization conditions: 121°C, 30min;
[0045] ② Take Aspergillus tabingensis preserved on the slant of the PDB test tube at 4°C for activation culture. Under a sterile environment, pick the strain from the slant of the test tube and inoculate it into a 250mL Erlenmeyer flask containing 150mL of sterilized Martin's medium in each bottle. The culture temperature is: 28°C; shaker culture 3d; speed 170r min -1 , to obtain the seed solution of the bacterial strain;
[0046] (2) Inoculate the seed solution of the activated bacteria in step (1) into sterile Martin's me...
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