In-situ remediation method of surface water polluted by hexavalent uranium using aspergillus tubingensis and phytate

An Aspergillus tabin, in-situ repair technology, applied in the field of uranium-contaminated surface water repair, can solve the problems of high treatment cost, complex process, difficult industrial application, etc., and achieves the effect of low repair cost and wide sources

Inactive Publication Date: 2019-04-16
NANHUA UNIV
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  • Application Information

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Problems solved by technology

However, these methods usually have disadvantages such as complicated process,...

Method used

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Experimental program
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Embodiment approach

[0025] Sediment samples were taken from a reservoir of a uranium tailings pond in Guangdong; according to calcium phytate 0.3%, MgSO 4 ·7H 2 O 0.05%, MnSO 4 4H 2 O 0.05%, FeSO 4 ·7H 2 O 0.003%, KCl 0.05%, NH 4 NO 3 0.05%, glucose 1.5% and agar powder 1.8% to prepare plate screening medium. Take 10 g of sediment, put it into a sterile Erlenmeyer flask filled with 90 mL of sterile normal saline and a few glass beads, place it on a shaker and vibrate at a speed of 150-180 r / min for 25-35 min, take it out, Make 10 each -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 and 10 -9dilute suspension. Spread the suspensions with different dilutions on the plate screening media with pH values ​​of 2, 3, 4, 5, 6, 7, 8, 9 and 10 respectively, and then put them into a constant temperature incubator, at 25~ Cultivate at a temperature of 35°C, and observe whether a hydrolysis circle occurs every 10-12 h. Pick colonies with a larger ratio of hydrolysis zone to colony diameter to inoculate...

Embodiment 1

[0029] Example 1: The hexavalent uranium-contaminated surface water was taken from a uranium tailings pond in southern China, with a uranium concentration of 280 μg / L and a pH value of 5.4. Take 1 L of hexavalent uranium to pollute the surface water, press [Na 12 -IP 6 ]:[U]=50:1 ratio, add 0.055 g sodium phytate to it, and inoculate 1 mL OD 600 The spore suspension of Aspergillus tubingensis with a value of 1.0 was cultured at 30°C for 62 days. After 62 days, the concentration of hexavalent uranium in the surface water polluted by uranium hexavalent dropped to 13 μg / L, the removal rate of hexavalent uranium reached 95.2%, and the pH value was 5.8. Separate the precipitate from the solution, neutralize the filtrate with a saturated milk of lime solution, adjust the pH value to be neutral, and discharge it.

Embodiment 2

[0030] Example 2: The hexavalent uranium-contaminated surface water was taken from a uranium tailings pond in southern China, with a uranium concentration of 220 μg / L and a pH value of 5.1. Take 1 L of hexavalent uranium to pollute the surface water, press [Na 12 -IP 6 ]:[U]=25:1 Add 0.028 g sodium phytate to it and inoculate 1 mL OD 600 The spore suspension of Aspergillus tubingensis with a value of 1.0 was cultured at room temperature for 70 days. After 70 days, the concentration of hexavalent uranium in the surface water polluted by hexavalent uranium dropped to 25 μg / L, the removal rate of hexavalent uranium reached 88.6%, and the pH value was 5.6. Separate the precipitate from the solution, neutralize the filtrate with a saturated milk of lime solution, adjust the pH value to be neutral, and discharge it.

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Abstract

The invention provides an in-situ remediation method of surface water polluted by hexavalent uranium using aspergillus tubingensis and phytate. The method comprises the steps that a modified Martin culture medium taking the phytate as the sole phosphorous source is added to the water body polluted by the hexavalent uranium, and aspergillus tubingensis spore suspension which is obtained by screening and separating from uranium mill tailing water reservoir sediments is inoculated. Under the threatening of uranium, and phosphatase is generated by the aspergillus tubingensis to promote the releasing of orthophosphate by the hydrolysis of the phytate. The orthophosphate and uranium in the water body are mineralized to generate a stable U-PO43 mineral, and therefore the remediation of the surface water polluted by the hexavalent uranium is achieved. The method has the advantages that the operation is simple, the remediation cost is low, the remediation effect is good and the interference onthe environment is low.

Description

technical field [0001] The invention relates to the field of remediation of uranium-contaminated surface water, in particular to a method for in-situ remediation of hexavalent uranium-contaminated surface water by using aspergillus tabin and phytate. Background technique [0002] During the process of uranium mining and uranium hydrometallurgy, a small amount of uranium will be released into the environment, causing some surface water to be polluted to a certain extent. Uranium in uranium-contaminated surface water can enter the human body through the food chain, and induce chronic radiation diseases in two ways: heavy metal toxicity and internal exposure. Therefore, it is an important task in the environmental field to study efficient remediation methods for uranium-contaminated surface water. [0003] The traditional treatment methods of uranium-contaminated surface water mainly include: ion exchange method, chemical precipitation method, evaporation concentration method,...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/14C12N1/02C12R1/66C02F101/10
CPCC02F3/347C02F2101/006C02F2103/007C12N1/02C12N1/14
Inventor 丁德馨张辉胡南王聂颖戴仲然王永东李广悦
Owner NANHUA UNIV
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