Dicumarol derivative as well as extraction method and application thereof

A technology of dicoumarin and extraction method, which is applied in the field of preparation of dicoumarin derivatives and achieves the effects of good antitumor activity and good cytotoxic activity

Active Publication Date: 2018-09-07
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on dicoumarin derivatives and their in vitro activities in endophytic fungi from feline feces

Method used

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  • Dicumarol derivative as well as extraction method and application thereof
  • Dicumarol derivative as well as extraction method and application thereof
  • Dicumarol derivative as well as extraction method and application thereof

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Embodiment Construction

[0033] Embodiments of the present invention are described in further detail below:

[0034] A kind of dicoumarin compound, the chemical structural formula of this compound:

[0035]

[0036] The extraction method of this compound is as follows:

[0037] (1) Carry out strain culture to cat feces endophyte Aspergillus tubingensis (Aspergillus tubingensis) DS37 in strain culture medium;

[0038] (2) In the fermentation medium, the bacterial classification obtained in step (1) is fermented and cultivated, and the fermentation product (9.2kg) is used as raw material to extract 8 times with petroleum ether / ethyl acetate / methanol, and the combined extracts are filtered , reclaim mixed solvent, concentrate under reduced pressure, obtain crude product extract 1500g;

[0039] (3) Put the crude product extract obtained in step (2) on a silica gel chromatographic column, and use petroleum ether with a volume gradient of 1:0:0, 0:1:0, 0:1:1, and 0:0:1 respectively / Ethyl acetate / meth...

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Abstract

The invention discloses a dicumarol derivative as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) carrying out strain culture on decaisnea insignis endophytic fungus aspergillus tubingensis DS37 in a strain culture medium; (2) carrying out fermentation culture on a strain obtained by step (1) in a fermentation culture medium; then extracting for a plurality of times by utilizing petroleum ether, ethyl acetate and methanol; combining extracting solutions and filtering; recycling a mixed solvent and decompressing and concentrating filtrate to obtain crude extract; finally, carrying out silica-gel column chromatography to obtain the dicumarol derivative. The dicumarol derivative prepared by the invention has good effects of inhibiting pseudomonas aeruginosa, streptococcus lactis and alternaria alternata and can be used for agricultural prevention and control and pesticide prevention and control technologies; the dicumarol derivative has relatively strong activity of resisting A549, MCF-7 and HepG2 cell lines and provides possibility for developing anti-tumor medicines.

Description

technical field [0001] The invention relates to the field of preparation of dicoumarin derivatives, in particular to a dicoumarin derivative and its extraction method and application. Background technique [0002] Decaisnea insignis (Griff.) Hook.f. & Thomson) is a plant of the genus Decaisnea insignis (Lardiyabalaeeae). Clumping deciduous erect shrubs, widely distributed in southwestern and central China. Akebia plant cat's feces is a traditional folk Chinese herbal medicine in my country. It tastes pungent, sweet, and flat in nature. The root and fruit are sweet and cool. So far, research on cat feces has mainly focused on its phytochemical components, while relatively little research has been done on its endophytic fungi. According to reports, the main types of secondary metabolites of endophytic fungi include: alkaloids, terpenes, steroids, organic acids and other compounds, all of which have certain biological activities. However, there are no reports about dicoumari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/54A61P35/00A61P31/04A61P31/10C12P17/06C12N1/14C12R1/66
CPCA61P31/04A61P31/10A61P35/00C07D311/54C12N1/14C12P17/06
Inventor 杨秀芳王宁宁陈镝康祎璠马养民雒佳欣史碧波龙园园
Owner SHAANXI UNIV OF SCI & TECH
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