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Efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof

A technology of Aspergillus tabbingen and phosphorus dissolving, which is applied in the field of microorganisms to achieve the effect of strong phosphorus solubility and good compatibility

Inactive Publication Date: 2017-02-22
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, resulting in more than 95% of insoluble phosphorus sources in the soil cannot be directly absorbed and utilized by plants

Method used

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  • Efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof
  • Efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof
  • Efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1, the isolation and identification of Aspergillus tubingensis (Aspergillus tubingensis) bacterial strain P2-2

[0058] 1. Isolation of Aspergillus tubingensis strain P2-2

[0059] 1. Preliminary screening of microorganisms in soil

[0060] Soil (soil from soybean fields in Changping, Beijing (E116°07.909′; N 40°11.303′)) was prepared to obtain 10 -2 -10 -4 suspension, and then spread the soil suspension on Ca 3 (PO 4 ) 2 On the insoluble inorganic phosphorus solid medium containing streptomycin (100 μg / ml) and chloramphenicol (100 μg / ml) as the phosphorus source, cultivate at 25 ° C, observe, isolate and purify the bacterial strain that produces the phosphorus-dissolving circle, and put The isolated and purified strain was named P2-2, and the strain was preserved at -80°C with 15% glycerol for future use.

[0061] 2. Identification of strain P2-2

[0062] 1. Morphological observation of strain P2-2

[0063] After the strain P2-2 was cultured on CYA m...

Embodiment 2

[0068] Embodiment 2, bacterial strain P2-2 to Ca 3 (PO 4 ) 2 , Zn 3 (PO 4 ) 2 , Hydroxyapatite as the phosphorus source of the dissolution of inorganic phosphorus medium

[0069] 1. The effect of strain P2-2 on Ca 3 (PO 4 ) 2 , Zn 3 (PO 4 ) 2 , Solubilization of solid inorganic phosphorus medium with hydroxyapatite as phosphorus source

[0070] The bacterial strain P2-2 is made into a bacterial suspension (the bacterial suspension is a solution obtained by dissolving the spores of the bacterial strain P2-2 in water), so that the number of spores in the bacterial suspension reaches 1×10 6 cfu / ml. Take 200 μl of bacterial suspension in a 6cm petri dish, and pour 5 glass beads into each petri dish to evenly coat the plate. After culturing at 25°C for 2 days, use a 6mm hole puncher to make fungus cakes, and insert the fungus cakes with Ca 3 (PO 4 ) 2 , Zn 3 (PO 4 ) 2 , hydroxyapatite as the phosphorus source on a solid inorganic phosphorus plate, cultured at 25°...

Embodiment 3

[0083] Embodiment 3, the affinity test of bacterial strain P2-2 and functional bacterial strain

[0084] Paecilomyces lilacinus YES-2 (YES-2), Streptomyces rubrum HDZ-9-47 (HDZ-9-47) and Paleomyces pallidum NC1 (NC1) are all functional complex microorganisms for controlling cucumber root-knot nematode .

[0085] 1. Mix and sterilize PDA, Gaoshi No. 1 and LA medium according to the same mass ratio and pour them into a 9cm petri dish to obtain a mixed medium. After the mixed medium is solidified, mix Streptomyces rubrum HDZ-9-47 (HDZ-9-47) and Pallidum NC1 (NC1) cells were inoculated into the mixed medium in the form of "well" streaks, and cultured at 30°C for 2 days; then the strains P2-2 and Take a 0.6cm cake from the edge of the Paecilomyces lilacinus YES-2 (YES-2) colony, and insert them into Streptomyces rubrum HDZ-9-47 (HDZ-9-47) and Pallidum NC1 (NC1) respectively. At the position of 0.5cm of the bacteria, observe the biocompatibility among the strains after inverting a...

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Abstract

The invention discloses an efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof. An efficient phosphorus-dissolving fungus is separated and purified from soil and is capable of activating various refractory phosphorus sources, inorganic phosphorus in decomposition solution is converted into effective phosphorus usable by plants, and the effective phosphorus is applicable directly to the preparation of plant nutrient solutions. Experiments prove that the phosphorus-dissolving fungus herein has high phosphorus-dissolving characteristics, is good in compatibility with a group of functional composite microbial colonies, and is also applicable to the research and development of composite microbial fungus fertilizers.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a strain of Aspergillus tubingensis and its application in dissolving inorganic phosphorus. Background technique [0002] Phosphorus is one of the three major mineral nutrients necessary for plant growth and development. Phosphorus not only participates in many important life metabolic activities, but also is a component of many plant organs. Phosphorus plays an important role in the transportation, synthesis and decomposition of crop carbohydrates. It can be seen that the abundance of phosphorus in the soil has a very obvious impact on the yield and quality of crops. Since most of the phosphorus and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, resulting in more than 95% of insoluble phosphorus sources in the soil cannot be directly absorbed and utilized by plants. [0003] There are a large number of phosphorus-solubilizing microo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08A01N63/04A01P21/00C12R1/66
CPCA01N63/30C05F11/08C12N1/145C12R2001/66
Inventor 向梅春刘杏忠杨顺杨婷
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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