234 results about "Aspergillus zonatus" patented technology

The invention discloses Aspergillus niger ML-0016 strain and application, which is characterized by the following: the reserving number is CCTCC.NO.M206034; the soy isoflavone glycoside is converted by strain to manufacture soy isoflavone glycosyl and aglycone.

The invention relates to preparation methods and uses of two dioxypiperazine compounds. The invention adopts aspergillus effuses H1-1 from root mud of Fujian mangrove forest to product novel structural dioxypiperazine compounds. Tests prove that: the compounds can be used as cellular breeding depressant or anti-tumor agent.

A composition used to treat diabetes is disclosed. The composition is made by first, mixing an isolated embryo bud and isolated seed coat from rice with a fruit body of Ganoderma lucidum to form a mixture. Next, the mixture is fermented with an Aspergillus oryzae strain to produce a resulting fermentate. Then, the resulting fermentate is dried to produce a dried fermentate. Next, the dried fermentate is crushed into a fine powder and lastly, the powder is mixed with vitamin B to form the composition.

The invention discloses an ultrahigh temperature decomposition agent of straws and cattle manure, and a preparation and an application of the decomposition agent, and belongs to the field of decomposition agents. The decomposition agent is prepared by mixed fermentation and drying of the straw, the cattle manure and a complex decomposition inoculant, wherein the complex decomposition inoculant comprises 1-10 parts of thermophilic bacterium inoculant, 1-6 parts of bacillus licheniformis inoculant, 1-6 parts of aspergillus oryzae inoculant, 1-6 parts of lactobacillus plantarum inoculant, 1-6 parts of thermoactinomyces sacchari, and 1-6 parts of cold-proof brevibacterium inoculant. The decomposition agent, a composting method and the application of the decomposition agent solve the problems of slow temperature rise during composting and decomposition, low fermentation temperature, long fermentation time, incomplete decomposition, poor compost quality and the like in the fermentation of the existing complex inoculant, and the decomposition agent is quick in temperature rise during the composting and the decomposition, quick in heating-up, high in fermentation temperature, short in fermentation time, more thorough in decomposition and higher in quality.

The invention discloses a microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and a preparation method thereof. The microbial feed additive comprises the following components by weight: 1-96% of lactobacillus acidophilus, 1-96% of bacillus subtilis, 1-96% of saccharomyces cerevisiae, 1-96% of aspergillus niger, and 1-96% of aspergillus oryzae. The preparation method comrpises: performing solid state fermentation of production strains obtained by performing solid state fermentation of mother strains so as to obtain the above strains, mixing the strains according to a certain proportion to obtain the microbial feed additive. The preparation method of the invention is safe and reliable, low in cost, energy-saving and environment-friendly, simple and controllable in process, and convenient for use, and realizes a livestock and poultry product with low fat content, delicious taste, safety and reliability; the microbial feed additive of the invention has unique compatibility, can substitute antibiotics and arsenic preparations, puts an end to clenbnterol hydrochloride and melamine, enables the increase of livestock and poultry product quality after used, improves meat egg milk flavor, and generates pollution-free green food.

The invention relates to the field of bioengineering, in particular to a preparation method and an application of a microbial agent composition for enhancing secondary fermentation of a bean paste. The microbial agent composition consists of a saccharomycetes agent, an aspergillus oryzae agent and a pleospora agent; and the three microbial agents are prepared, and then the prepared microbial agents are mixed with the secondary-fermented bean paste, so as to promote the secondary fermentation of the bean paste. The whole process of the preparation method of the microbial agent composition provided by the invention is applicable to continuous industrial production; by-products (chili and radix platycodonis), from the production of Pixian bean paste, are comprehensively utilized; a production cycle can be shortened by 6 months, the content of amino nitrogen can be improved by 20% and the content of volatile aroma-producing components can be improved by more than 3 times (the contents of total ester, total acid and total aldehyde); by inoculating aflatoxin B1 with the saccharomycetes agent at a production peak (after being fermented for 30-60 days), an ester producing and aroma generating process can be enhanced, the metabolism of aspergillus flavus and partial aspergillus parasiticus can be competitively inhibited and the content of the aflatoxin B1 can be reduced, and the content of the aflatoxin B1 is lower than 0.5ppm, so that food safety is enhanced.

The invention provides a mulberry leaf feed as well as a processing method and application of the mulberry leaf feed. According to the mulberry leaf feed, dry mulberry leaf powder is artificially fermented by virtue of screened mixed bacteria of saccharomycetes, lactobacilli and aspergillus oryzae, so that the mouth feel of the mulberry leaf powder can be well improved, the ingestion capability of feed animals can be improved, and the apparent digestibility of the feed can be improved.

The invention discloses a method for preparing ginseng rare saponin by virtue of microorganism enzymatic conversion of ginsenosid Re. According to the method, aspergillus microorganisms are fermented to obtain an enzyme solution, and then the enzyme solution reacts with cheap ginseng with high contents of ginseng saponin Rb3, Rc, Rb2, Rd and the like or western stem and leaf glycol saponin or pseudo-ginseng steam and leaf saponin, so that a high-activity ginseng rare saponin C-Mx, C-Mc, C-Y, C-K mixture and monomers thereof are prepared. The method is low in cost, simple in process and free from pollution; products can be applied to ginseng products, functional foods and cosmetics.

The invention provides a liriope spicate endogenous aspergillus fungus and application thereof to preparation of steroid saponin, and relates to the technical field of microorganisms. The fungus mainly solves the problems that due to the factors that the liriope spicate steroid saponin is mostly and directly from liriope spicate extracts, the liriope spicate steroid saponin content is low, the saponin extraction cost is high, and the like, the market delivery quantity of the liriope spicate steroid saponin is limited. The aspergillus fungus is separated from living body tuberous roots of liriope spicata var. prolifera, and belongs to aspergillus sp. through 18S rDNA sequence identification; the aspergillus fungus is preserved in CCTCC (China Center for Type Culture Collection); the preservation data is December 7, 2016; the preservation number is CCTCC No.M2016721. The liriope spicate produced by a liriope spicate GAP (Good Agricultural Practices) base (the liriope spicate source areain Oumao town, Xiangyang city in Hubei province ) is used; the liriope spicate steroid saponin is generated through liriope spicate endogenous fungus plant liquid fermentation. The endogenous fungus is an important microorganism for finding a new resource of the liriope spicate steroid saponin.

PCT No. PCT / DK95 / 00511 Sec. 371 Date Aug. 14, 1997 Sec. 102(e) Date Aug. 14, 1997 PCT Filed Dec. 20, 1995 PCT Pub. No. WO96 / 19582 PCT Pub. Date Jun. 27, 1996A method of producing a milk clotting enzyme including the steps of (a) fermenting a strain of Rhizomucor miehei or Aspergillus oryzae to form a fermentation product having a glycoslated Rhizomucor miehei aspartic protease and other proteins, and (b) subjecting a quantity of the fermentation product to a deglycosylating treatment to form a coagulant preparation having an at least partly deglycoslated aspartic protease and the other proteins. The at least partly deglycosylated protease has a milk clotting activity that is at least 10% higher than a milk clotting activity of the glycosylated aspartic protease.

The invention discloses an aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of the aspergillus oryzae bacterial strain, and belongs to the technical field of biological fermentation. The bacterial strain for fermentation is specifically aspergillus oryzae DJ36, and is collected in the China General Microbiological Culture Collection Center with a collection number of CGMCC NO.13575. A polypeptide feed preparing process is implemented through a liquid fermentation technology in conjunction with an enzymatic hydrolysis technology, and specifically comprises the following steps: preparing a monospore bacterial suspension of the aspergillus oryzae; preparing a liquid bacterial strain; performing liquid fermentation with a triangular flask or a fermentation tank; performing enzymatic hydrolysis on impure protein with fermentation liquid; drying, smashing, packaging and the like. A functional small-molecular polypeptide is generated efficiently under the coaction of aspergillus oryzae fermentation and enzymatic hydrolysis at the later stage of fermentation, the advantages of a microbial fermentation technology and the enzymatic hydrolysis technology are combined, the use of an enzyme preparation is greatly reduced, the production cost of the polypeptide is lowered, and the industrialization process of the polypeptide is accelerated; moreover, a prepared feed additive has a relatively high nutrient value and disease resistance, and a wide application prospect.

The invention discloses high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance. The high-efficiency phosphate-solubilizing aspergillus japonicus with the heavy metal tolerance is classified and named as aspergillus japonicus, and is collected in China General Microbiological Culture Collection Center; the collection number is CGMCC No.7700. The invention also discloses the tolerance of the high-efficiency phosphate-solubilizing aspergillus japonicus to heavy metals. The high-efficiency phosphate-solubilizing aspergillus japonicus has an extremely good dissolving effect on insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and aluminum phosphate in the situation of liquid shake flask culture; the dissolving effects on the three types of insoluble inorganic phosphorus reach 100 percent. The aspergillus japonicus has certain tolerance to the heavy metals after being incubated in liquid culture solutions which contain the heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>; particularly, the tolerated concentrations of the Pb<2+>, the Zn<2+>, the Cr<2+> and the Mn<2+> reach 2,000 mg / L respectively. Therefore, the high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance can provide excellent strain resources for the development of heavy metal-resistant high-efficiency phosphate-solubilizing bacterium agents.

The invention discloses a method for preparing questin by utilizing an ocean aspergillus flavipes HN4-13 bacterial strain. The method comprises the following steps: conducting separation and purification on antibacterial active substances generated through fermentation of the aspergillus flavipes HN4-13 bacterial strain according to silica-gel column chromatography, Sephadex LH-20 column chromatography, preparation of high performance liquid chromatography (PHPLC) and the like by taking vibrio harveyi as an indicator bacterium, so as to obtain a vibrio harveyi-preventing active compound HY2; indentifying the structure of the active compound HY2 according to spectra data such as ESI-MS, 1H-NMR and 13C-NMR, so as to determine that the active compound HY2 is questin. The questin is obtained through separation of a metabolic product of aspergillus flavipes HN4-13; experiments show that the questin can perform a certain inhibiting function of the pathogenic bacteria, such as vibrio harveyi, vibrio anguillarum, vibrio parahaemolyticus and vibrio cholerae, of aquatic products, and can be used for preparation of medicine preventing pathogenic vibrio of the aquatic products.

The invention discloses a method for preventing bamboo from mildewing by using aspergillus flavus. By utilizing the characteristic that in a given period, aspergillus flavus consumes more starch and reducing sugar than common mold and the physical mechanical performance of the bamboo does not drop obviously, the method comprises the following steps: inoculating the aspergillus flavus to the bamboo, so that the contents of starch and reducing sugar in the bamboo rapidly drop; and finally removing dark mold spots on the surface of the bamboo by bleaching, so as to recover the beautiful color of the bamboo surface. The technique is composed of aspergillus flavus culturing technique, aspergillus flavus inoculating technique, aspergillus flavus sterilizing technique and mold-infected bamboo bleaching technique. As proved in bamboo mold resistance experiments performed according to ASTM: D4445-03 (Standard Test Method for Fungicides for Controlling Sapstain and Mold on Unseasoned Lumber (Laboratory Method)): the bamboo is subjected to aspergillus flavus pre-infecting and then bleaching, the mold resistance of the bamboo is remarkably improved and no mold grows in an experiment period of 30 days.

The invention discloses a compound fungicide FX for degrading straws. The compound fungicide FX comprises a Bacillus subtilis strain NJX501, a Bacillus subtilis strain NJX502, a Bacillus methylotrophicus strain NJXy and an Aspergillus fumigatus strain NJZ501. The compound fungicide is prepared by mixing the bacterium liquid of NJX501 with a bacterium OD value of 0.5-1, the bacterium fluid of NJX502 with a bacterium OD value of 0.5-1, the bacterium liquid of NJXy with a bacterium OD value of 0.5-1 and NJZ501 spore suspension with spore number of not less than 104 / ml in equivalent volume. The compound fungicide FX can be used for promoting the composting and decomposition of rice straws under high temperature conditions.

The invention relates to the field of daily chemical products, in particular to a plant source antibacterial agent and a preparation method thereof. The plant source antibacterial agent comprises thefollowing components in parts by weight: 20-33 parts of a pulsatilla cernua extract, 14-36 parts of a Chinese prickly ash extract and 20-38 parts of a tillandsia usneoides extract. The plant source antibacterial agent provided by the invention has a very high property of inhibiting growth of bacillus subtilis, escherichia coli, staphylococcus aureus, candida albicans, pseudomonas aeruginosa, aspergillus niger and the like, and a preservative property as well, besides, the stability of the agent can be also very well improved at a high temperature. In addition, cosmetics can be kept gentle whenantibacterial effects and high-temperature stability are maintained, and the agent has the characteristics of being non-toxic and non-irritant to human bodies.

The invention relates to the field of wine making, and particularly relates to strains with high cellulase activity as well as a screening method and a use method of the strains. The strains with high cellulase activity are aspergillus. The screening method comprises the following steps of: A. enriching and cultivating bacteria through an inorganic salt culture medium; B. carrying out once or multiple-time inoculation on the bacteria enriched and cultivated on filter paper dipped with the inorganic salt culture medium; C. acquiring a cultured product at the fracture part of the filter paper, and carrying out once or multiple-time streaking on the cultured product on a carboxymethylcellulose culture medium plate, so as to obtain pure strains; D. cultivating the pure strains obtained from the step C in the inorganic salt culture medium and carrying out fibrinolysis on the cultivated pure strains; E. inoculating the pure strains with high fibrinolysis capacity in the step D to a steriled solid fermentation medium for cultivating; and F. extracting coarse enzyme liquid by adopting a water bath method, and carrying out enzyme activity test to obtain the strains with high cellulase activity. The strains and bread yeast are compounded for use and fermented by taking wine waste lees and brans as main materials. With the adoption of the strains with high cellulase activity, the fermentation rate of the wine waste lees can be improved.

The invention discloses a method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori 1042-2 which belongs to the Aspergillus and has the collection registration number of M209142 in the China Center for Type Culture Collection (CCTCC). The method for producing the cellulose and the polypeptide protein feed comprises the following steps: A, selecting the Aspergillus awamori 1042-2; B, authenticating the Aspergillus awamori 1042-2, C, culturing the Aspergillus awamori 1042-2 liquid; D, fermenting the solid material; and F, postprocessing. The solid culture which is obtained by inoculating the Aspergillus awamori 1042-2 onto the solid culture medium and fermenting the Aspergillus awamori 1042-2 and the solid culture medium is baked to dry, ground, sieved and subjected to the detection of the enzyme activity and the polypeptide protein content. The method for producing the cellulose and the polypeptide protein feed is feasible and easy, the produced powdered cellulose has the CMC enzyme activity of 31397.1-41826.7 U / g and the FPA enzyme activity of 1779.3-2212.1 U / g, has good stability and can be produced on a large scale, and the produced polypeptide protein feed contains more than 35 percent of protein. The Aspergillus awamori 1042-2 can be used for fermenting the palm kernel dregs, other dregs and the vegetable fiber and producing the biological feed, the biological fertilizer, the biological fuel and can be also used in the food industry, chemical industry and the pharmaceutical industry. The polypeptide protein feed produced by fermenting with the Aspergillus awamori 1042-2 can be used as the additive of the protein of various feed.

The invention discloses a microbial additive for alfalfa haylage, a preparation method and an application thereof. The additive is prepared from a bacterial suspension of aspergillusoryzae and an MRS liquid nutrient medium for streptococcus lactis according to the ratio of 50-70%: 50-30% in weight percent, wherein per milliliter of the bacterial suspension of the aspergillusoryzae at least contains 1.0*108 aspergillusoryzae spores, and per milliliter of the MRS liquid nutrient medium at least contains 4.0*108 cfu streptococcus lactis. The microbial additive quickens the initial fermentation rate of lactobacillus, enhances acid production capability, effectively inhibits the activity of saccharomycetes and other undesirable microorganism, strengths haylage effect and effectively promotes the production performance of cows, beef cattle, mutton sheep and swine. The strain adopted by the invention can be obtained easily, can be produced massively on application and has practicability.

The invention discloses a method for preparing a special composite microbial fertilizer for non-deciduous fruit trees and capable of improving soil, and a prepared product. The preparation method comprises the following steps of: deodorizing fresh chicken manure and dairy cow manure; adding sporotrichum thermophile, aspergillus niger, brown sugar, crushed straws, vinasse and monopotassium phosphate, fermenting and performing air drying to obtain a preparation I; fermenting bean pulp, wheat bran, calcium powder, brown sugar, bacillus megatherium, bacillus subtilis, lactic acid bacteria and ferrous sulfate, and performing air drying to obtain a preparation II; and mixing the preparation I and the preparation II, adding bacillus megatherium, bacillus subtilis, aspergillus niger, streptomycete, terricolous cyanobacteria, photosynthetic bacteria and dinoflagellate, fermenting, and adding chemical fertilizers needed for growth of the non-deciduous fruit trees to obtain the special composite microbial fertilizer for the non-deciduous fruit trees. The prepared product contains viable beneficial microorganisms, and can be used for improving soil aggregated structure and other indexes, and increasing the yield of fruits.

The invention discloses an efficiently phosphorus-dissolving Aspergillus tubingensis strain and application thereof. An efficient phosphorus-dissolving fungus is separated and purified from soil and is capable of activating various refractory phosphorus sources, inorganic phosphorus in decomposition solution is converted into effective phosphorus usable by plants, and the effective phosphorus is applicable directly to the preparation of plant nutrient solutions. Experiments prove that the phosphorus-dissolving fungus herein has high phosphorus-dissolving characteristics, is good in compatibility with a group of functional composite microbial colonies, and is also applicable to the research and development of composite microbial fungus fertilizers.

The invention discloses an Aspergillus niger C2 of which the collection number is CGMCC NO.7511 and the collection date is July 5th, 2013. The Aspergillus niger C2 is separated from farmland soil in Hengshui Cicy Hebei Province in China, and has been collected at China General Microbiological Culture Collection Center; and the address is Institute of Microbiology in Chinese Academy of Sciences, Beichenxi Road Yard 3 No.3, Chaoyang District, Beijing City. The invention also discloses a microbial inoculum containing the Aspergillus niger C2. The microbial inoculum disclosed by the invention has obvious growth promotion effect on plants without being inhibited by the application of the fertilizer, can be used under the condition of normally using the fertilizer, and has important meanings for phosphate fertilizer resource saving for Chinese agricultural production, continuous yield increase of grains and protection of ecological environment of farmland.

The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.

The invention belongs to the field of biologics and in particular relates to a synchronous detection RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for the yield of aflatoxin and the quantity of Nor-1 genetic transcription and a detection method of the kit. The invention establishes a synchronous RT-PCR detection method for synchronously detecting the yield of aflatoxin and the quantity of Nor-1 genetic transcription, and by using the method, the yield of the aflatoxin and the quantity of Nor-1 genetic transcription are in good linear relationship with results of HPLC (High Performance Liquid Chromatography) quantitative aflatoxin and Nanodrop quantitative Nor-1 genetic transcription, therefore, AFT-Nor-1 obtained by using the synchronous detection RT-PCR method has reliableand accurate ratio results which can be used as identification indexes for judging the toxin yields of aspergillus flavus strains, rapid and accurate identification evaluation on the toxin yields of the aspergillus flavus strains is achieved, and the kit has great significances for early warning and control on aflatoxin pollution.

The invention discloses a triazole antifungal compound and its preparation method and application, belongs to the field of pharmaceutical compounds, and specifically relates to a new triazole antifungal compound with the following general chemical structure formula. The preparation method of the compound of the present invention is economical, simple, mild and effective. The obtained compound has strong antibacterial activity against pathogenic fungi Candida albicans and Aspergillus, compared with clinical drugs fluconazole and itraconazole, it has the advantages of high efficiency and broad spectrum, and can be used to prepare new antifungal agents drug.

The invention discloses a preparation method of a biological compound fertilizer. The method comprises the following steps: weighing the following raw materials, in parts by weight: 10-15 parts of a composite biological bacteria preparation, 6-10 parts of modified biochar, 5-10 parts of an amino acid liquid, 20-30 parts of urea, 3-5 parts of ammonium sulfate, 0.5-1.5 parts of calcium phosphate, 5-10 parts of a microelement nutrient solution, and 25-35 parts of glycerol. According to the method provided by the invention, the modified biochar improves soil saturated hydraulic conductivity, can effectively reduce occurrences of surface runoff and soil erosion, the modified biochar can promote decomposition of the urea and the ammonium sulfate, so that the fertilizer can more quickly work; thecomposite biological bacteria preparation comprises a microcapsule outer shell and biological bacteria, after the modified biochar and the composite biological bacteria preparation are mixed, the mixed mixture can provide more habitat for survival of aspergillus niger and rhizobia, and promote the action of the aspergillus niger and the rhizobia on crops.

The invention belongs to the field of biotechnology microorganisms, and particularly relates to an Aspergillus clavatus-32 strain producing Lovastatin through liquid fermentation, application and a fermentation producing method. The Aspergillus clavatus-32 strain is high in Lovastatin yield and growing speed, large in sporulation quantity and simple to culture. Under the conditions that initial pH of a fermentation culture medium is 5.2 and culture temperature is 28 DEG C, after the liquid fermentation culture medium and fermentation conditions are optimized and the Ac-32 strain is fermented in a 5L fermentation tank for 110-140h, yield of Lovastatin can reach about 200-250mg / L.

The invention belongs to the technical field of herbicides, and discloses an aspergillus tubingensis L-27 strain of which a fermentation broth has herbicidal activity, the fermentation broth and an herbicide which are prepared from the strain, and an enrichment method and an identification method of herbicidal active components in the aspergillus tubingensis L-27 strain fermentation broth. By screening herbicidal activities of fermentation broths of 30 soil fungus strains, phenomena are found that inhibition rates of the aspergillus tubingensis L-27 strain fermentation broth on roots and stemsof amaranthus retroflexus, descurainia sophia and portulaca oleracea reach 100%; and regarding pharbitis purpurea, only stems grow weakly, and an inhibition rate still reaches 77.8%. A weak-base anion-exchange resin D301 is screened out to serve as an enrichment resin of the herbicidal active components, and after adsorption, growth inhibition rates of a residual liquid on roots and stems of wheat are only 1% and 7% respectively; and finally, a 100% ethanol solution of 0.1 mol / L HCL is determined to serve as a desorption agent of the active components enriched by the D301 resin. The herbicidal activity of the fermentation broth of the L-27 strain has potential development value.

The invention discloses an amaranthus hypochondriacus compound forage wrapping bag. The amaranthus hypochondriacus compound forage wrapping bag mainly comprises a batch and a microbe preparation of which the ratio is (92-96):(4-8) in parts by mass, wherein the batch consists of amaranthus hypochondriacus, pennisetum sinese roxb, maize straw, mountain flour, calcium hydrophosphate and dairy salt; the microbe preparation consists of a lactobacillus acidophilus fermentation fungicide, a bacillus subtilis fermentation fungicide, a beer yeast fermentation fungicide, an aspergillus niger fermentation fungicide and an aspergillus oryzae fermentation fungicide. The invention also discloses a preparation method and application of the amaranthus hypochondriacus compound forage wrapping bag, and a pig raising bed made of the amaranthus hypochondriacus compound forage wrapping bag. The amaranthus hypochondriacus compound forage wrapping bag can serve as not only a pig raising feed but also a raw material of a pig raising bed, is safe, healthy, nutrient and delicious and added with no antibiotics when serving as an ecologic pig raising feed, is strong in excrement and urine decomposition capability and good in piggery odor removal effect when serving as the raw material of the pig raising bed.

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.