55 results about "Aspergillus zonatus" patented technology

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses a nucleic acid product, a kit and a method for detecting aspergillus and application of the nucleic acid product, the kit and the method in detecting mildewed tobacco leaves. The nucleic acid product comprises a primer pair and a probe for detecting aspergillus, the primer pair comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2, and the nucleotide sequence of the probe is as shown in SEQ ID NO: 3. The nucleic acid product can specifically detect aspergillus, can realize rapid detection of tobacco mildew based on qPCR technology, has high sensitivity, simple operation, low cost and fast detection speed, can complete the whole detection process within several hours, does not need uncapping in amplification and detection processes, and avoids aerosol pollution caused by uncapping detection in common PCR.

The invention discloses a nucleic acid composition for detecting fungi as well as a kit and a detection method thereof, and relates to the technical field of molecular diagnosis. The embodiment of the invention provides a group of nucleic acid compositions, the compositions comprise primer pairs 1-5, the compositions can realize synchronous detection of aspergillus, cryptococcus, pneumocystis yarrowii and mucor on the basis of a real-time fluorescent quantitative PCR technology, and the nucleic acid compositions have the remarkable advantages of short detection time, simplicity and convenience in operation, high sensitivity, strong specificity and the like.

The invention discloses a modified chitosan-based antibacterial material as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The ciprofloxacin grafted quaternized chitosan is successfully synthesized by taking the chitosan as a raw material, and the water solubility and the antibacterial ability of the chitosan are effectively improved. The antibacterial diameters of the ciprofloxacin grafted quaternized chitosan prepared by the preparation method disclosed by the invention on escherichia coli and staphylococcus aureus are respectively 35.34 to 39.06 mm and 40.45 to 44.51 mm, and the minimum MIC (Minimum Inhibitory Concentration) and MBC (Minimum Mass Chitosan) can reach 0.0005 mg / mL. In addition, the antibacterial material has an obvious inhibition effect on colletotrichum gloeosporioides and aspergillus niger, and the highest antifungal rates respectively reach 53.81 + / -2.17% and 54.88 + / -1.3%. Compared with the raw materials, the antibacterial ability is greatly improved, and the grafted and modified composite material is environment-friendly and can be used for antibacterial food packaging materials.

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The application discloses an Aspergillus specific gene and a detection method and application thereof, and belongs to the technical field of biotechnology and medical molecular diagnosis. The nucleotide sequence comprises a full length or a partial continuous fragment selected from any one of sequences shown in SEQ ID NO. 1 to SEQ ID NO. 20, or a complementary sequence thereof. In the detection, nucleic acid is extracted from a sample to be detected; a primer pair is used to perform a nucleic acid amplification reaction on the extracted nucleic acid; the presence or quantity of an amplification product is detected, and a real-time Ct value is analyzed; a critical value is determined based on a test on a known negative sample library; and whether Aspergillus is detected is determined according to the Ct value and the critical value. The application shows high specificity, sensitivity and repeatability in clinical sample detection, and the detection period can be shortened to within 4 hours, thereby solving problems of long detection period, low sensitivity and cross reaction of existing Aspergillus detection methods.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The application relates to the technical field of biological medicine, in particular to a preparation method of anti-aspergillus niger siRNA and a complex preparation thereof, wherein the anti-aspergillus niger siRNA comprises siR266 for a PmrA gene and / or siR505 for a SarA gene, the sequence of a sense strand and an antisense strand of the siR266 is shown in SEQ ID NO: 3-4 respectively, and the sequence of a sense strand and an antisense strand of the siR505 is shown in SEQ ID NO: 7-8 respectively. Wherein, the complex preparation adopts protamine sulfate as a carrier, and forms a complex with the anti-aspergillus niger siRNA. The complex preparation prepared by the application can effectively inhibit aspergillus niger, and can be applied to food preservation, postharvest protection of fruits and vegetables, and mildew prevention of daily necessities and the like, and is suitable for developing corresponding anti-fungal products.

The invention provides a bacterial strain for preventing and treating tobacco black shank, which is a new aspergillus strain Aspergillus candidus, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO.40802. The invention also provides a preparation method of the bacterial strain for preventing and treating tobacco black shank. The obtained MFZ2320 strain with biological activity on tobacco black shank can be applied to prevention and treatment of tobacco black shank on tobacco, the bacteriostatic activity of a crude extract is tested by adopting an agar diffusion method, and a result shows that when the concentration of a secondary metabolism crude extract of the marine fungus MFZ2320 strain is 10mg / mL, the bacteriostatic diameter on tobacco black shank reaches 16.67 mm, the inhibition rate is 37.04%, and the marine fungus MFZ2320 strain can be applied to prevention and treatment of tobacco black shank. The obvious antibacterial effect is shown.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

This invention discloses a method and kit for rapid detection of deeply infected Aspergillus. The method involves mixing the sample to be tested with a nucleic acid cleavage extract and heating at 60–65°C for 8–20 min, followed by heating at 93–98°C for 5–10 min to obtain a DNA extract. This DNA extract is then added to a PCR premix containing a composition of deeply infected Aspergillus to prepare a PCR reaction solution. The PCR reaction solution is subjected to the following PCR amplification reactions: denaturation is performed at 90–100°C for 0–5 s, followed by annealing and extension at 50–70°C for 0–5 s, repeated 35–50 times. This method not only provides a specific primer and probe composition capable of distinguishing between Aspergillus flavus, Aspergillus niger, and Aspergillus fumigatus, but also enables rapid detection of the sample from nucleic acid extraction to nucleic acid amplification, saving detection time.

This invention discloses a genetically engineered bacterium lacking the acetyltransferase gene asp12, its construction method, and its applications. This invention utilizes CRISPR / Cas9 to knock out the asp12 gene encoding acetyltransferase in Aspergillus terreus strain GZU-31-1, constructing the genetically engineered bacterium Δasp12. This invention found that compared to the wild-type Aspergillus terreus strain GZU-31-1, the genetically engineered bacterium Δasp12 exhibits increased accumulation and significantly higher yield of the compound Aspermeroterpene F, which possesses anti-inflammatory and anti-hepatic fibrosis activities. Based on the genetically engineered bacterium Δasp12, this invention also provides a method for preparing the compound Aspermeroterpene F, further improving the yield of the compound by optimizing the fermentation culture medium. This invention is beneficial for the development of anti-inflammatory and anti-hepatic fibrosis drugs.

The application discloses a biosynthesis gene cluster of a polyene macrolide natural product mandimycin, and a natural product and application of the biosynthesis gene cluster, wherein the nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO. 1, and the compound structural formula of the natural product mandimycin and mandimycin B is shown as formula I and formula II. The natural product mandimycin can target fungal cell membrane phospholipid molecules, especially phosphatidylinositol, cause important ion efflux in fungal cells, and lead to fungal cell death. The natural product mandimycin and mandimycin B have strong in-vivo and in-vitro antibacterial activity and a broad antibacterial spectrum on various WHO-published multiple drug-resistant fungal key pathogens, including candida, aspergillus, cryptococcus, mucor and fusarium.

The invention discloses aspergillus niger, a method for preparing graphene by parasitic graphite of the aspergillus niger and an industrial production method of the graphene, and belongs to the technical field of graphene preparation. According to the three-stage fermentation-centrifugal separation coupling system, graphene stripping is achieved through parasitism of aspergillus niger in graphite gaps, then filtering separation, ultrasonic stripping and impurity removal are conducted, graphene is obtained, and the invention further discloses an industrial production method of the three-stage fermentation-centrifugal separation coupling system for preparing graphene through aspergillus niger parasitism graphite. Aspergillus niger parasitism is utilized, the problems existing in preparation of graphene through a chemical oxidation-reduction method are reduced to a great extent, the preparation process is environmentally friendly, operation is easy, the action condition is mild and easy to control, and the method has the advantages that the fermentation condition is mild, the preparation process is clean and free of pollution, and a large amount of waste liquid is not generated; and the method has wide industrial application value and significance.

The invention is applicable to the technical field of microorganisms, and provides aspergillus flavus and application thereof in prevention and treatment of fall webworm, the aspergillus flavus is aspergillus flavus YZNUH-01, and is preserved in China General Microbiological Culture Collection Center on February 11, 2025, the address is No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 41809. The specific high pathogenicity of the aspergillus flavus YZNUH-01 to the fall webworm is explored and verified for the first time, brand new fungus resources with different action mechanisms are provided for biological control of the fall webworm, and the aspergillus flavus YZNUH-01 is good in control effect and environmentally friendly.

The invention relates to a five-membered heteroaryl compound and application thereof, the compound is a compound with a structural formula shown in the specification or pharmaceutically acceptable salts and prodrugs thereof, in the formula, R1 is selected from CH3 and H; r2 is selected from NH2 and OH; r3 is selected from CH3 and H; r4 is selected from OH, F and H; r5 is selected from NO2 and H; r6 is selected from CN, NO2 and H; and L1 is selected from-CH2O <-> and-CH2-CH2 <->, and-CH is equal to CH <->. A series of five-membered heteroaryl compounds are researched and developed on the basis of an APX001A structure, and the five-membered heteroaryl compounds have a good Gwtl protein binding effect and can inhibit Gwtl protein activity, so that GPI-AP synthesis is prevented, mannose glycoprotein on the fungal surface cannot be cross-linked to a cell wall, the host surface adhesion capacity and cell wall integrity of the mannose glycoprotein are destroyed, the antifungal effect is achieved, and the application prospect is broad. The compound has good inhibitory activity on candida, cryptococcus and aspergillus.

The application provides a set of primers and probes for detecting invasive fungi, reagents and application thereof, a kit and a detection method, and relates to the technical field of biology.The nucleic acid sequence of the primer is shown as SEQ ID NO.1-2, 4-5, 7-11, 14-15, 17-18 and 20-21; and the nucleic acid sequence of the probe is shown as SEQ ID NO.3, 6, 12-13, 16, 19 and 22.The primers and probes have high specificity and sensitivity, and can be used for the detection and identification of Aspergillus, Mucorales, Cryptococcus neoformans, Pneumocystis jirovecii and Lichtheimia. The kit for detecting invasive fungi has high specificity and sensitivity, and wide fungal coverage, and can realize the detection of multiple invasive fungi simultaneously.

This invention discloses a method for preparing camphor root volatile oil, which is used for anti-oxidation, anti-aspergillus flavus, and inhibition of aflatoxin synthesis. The camphor root volatile oil of this invention is extracted by steam distillation, with a maximum volatile oil content of 3.34%. Gas chromatography-mass spectrometry analysis of the chemical composition of the camphor root volatile oil confirmed that the main components include camphor, 1,8-cineole, linalool, α-pinene, and sapinene. When applied to inhibit the growth and toxin production of Aspergillus flavus, a volatile oil concentration of 3 μL / mL completely inhibits aflatoxin production. This volatile oil also possesses antioxidant activity, with a half-maximal inhibitory concentration (IC50) for scavenging ABTS free radicals. 50 Below DPPH radicals, its lowest IC50 value is... 50 The values ​​were 0.6115±0.4240 mg / mL and 1.7658±0.2488 mg / mL, respectively. This volatile oil can also inhibit and control Aspergillus flavus contamination in Job's tears, and has application value in the food and health industries.

The invention relates to the technical field of microorganisms, and particularly provides Aspergillus terreus DZ-Q1-1 and an application of the Aspergillus terreus DZ-Q1-1 in salt tolerance. The strain is an endophytic fungus separated from the root system of a halophyte, namely, sedum lineare, and is preserved in the China Center for Type Culture Collection on August 21, 2025, and the preservation number is CCTCC NO: M 20251873. Experiments show that the strain can still grow well in a culture medium containing 0.75 M of NaCl and shows strong salt tolerance. The strain and corn seedlings are co-cultured, so that the growth indexes such as plant height, root length and fresh weight of corn under salt stress can be remarkably improved, the activity of superoxide dismutase and peroxidase and the content of chlorophyll are enhanced, and cell membrane damage is reduced. The invention provides a new microbial resource for improvement of salinized soil and improvement of plant salt tolerance, and has important agricultural application value.

The invention belongs to the technical field of biology, and particularly relates to bacillus amyloliquefaciens and application thereof in disease resistance and growth promotion of peanuts. The bacterial strain number is BAZX-2, the bacterial strain is preserved in the China General Microbiological Culture Collection Center on January 9, 2026, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.37345. The bacterial strain provided by the invention has the antibacterial rate of 61.1%-77.11% for three pathogenic bacteria, namely aspergillus niger, sclerotium rolfsii and / or fusarium oxysporum, and the antibacterial rate of the bacterial strain provided by the invention is 8.1%-87.11% for three pathogenic bacteria, namely aspergillus niger, sclerotium rolfsii and / or fusarium oxysporum. Wherein the bacteriostatic effect of thallus treatment is obviously superior to that of fermentation liquor and sterile filtrate; bAZX-2 fermentation liquor can obviously improve germination indexes, germination potential and germination rate of three peanut varieties, and the maximum improvement amplitude of the germination indexes reaches 34.33%, 23.29% and 27.47% respectively; the invention proves that BAZX-2 has dual functions of disease resistance and growth promotion, can simultaneously prevent and treat various diseases of peanuts, and provides excellent strain resources and technical support for green prevention and control of the diseases of the peanuts.

This invention provides a compound microbial agent for improving saline-alkali soil, its preparation method, and its application, belonging to the field of microbial technology. Specifically, the compound microbial agent comprises the following components by weight: 20-30 parts of Bacillus subtilis, 10-15 parts of Bacillus licheniformis, 10-20 parts of Acinetobacter calcium acetate, 10-20 parts of Bacillus belyssus, 5-10 parts of Aspergillus niger, 1-3 parts of citric acid, 20-30 parts of biochemical potassium humate, 10-20 parts of water, 50-60 parts of rice husk, 2-5 parts of pig manure, 10-20 parts of fish meal, and 10-20 parts of soybean meal. The compound microbial agent provided by this invention promotes potato growth in saline-alkali soil environments, increases the water content of potato leaves, and improves the physical and chemical properties of the soil.

The invention belongs to the technical field of cosmetics, and particularly relates to a plant composition for controlling oil and preventing hair loss as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning, airing, crushing and sterilizing a mixed raw material of radix polygoni multiflori preparata, fructus ligustri lucidi, tribulus terrestris and ginseng to obtain a pretreated solid-state fermentation raw material; sequentially adding an aspergillus oryzae suspension and a monascus purpureus suspension into the pretreated solid-state fermentation raw material for step-by-step fermentation culture, extracting the fermented raw material with an ethanol aqueous solution, concentrating an extracting solution, and redissolving the concentrated extracting solution with pure water to obtain a solution A; and heating and extracting mixed raw materials of cacumen biotae, crocus sativus, lithospermum erythrorhizon, ligusticum wallichii and radix sophorae flavescentis by adopting the solution A as an extraction solvent to obtain an extracting solution, namely the plant oil-control anti-hair loss composition. The composition obtained by the invention is proved to have remarkably improved oil control effect and anti-hair loss effect.

The present application relates to the field of biotechnology, disclose a kind of biological remediation method for removing sodium selenite in water body by aspergillus niger and bacillus combined culture, comprising the following steps: S1.in YPD culture medium inoculation activated aspergillus niger, shake culture, form aspergillus niger ball;S2.in LB culture medium inoculation bacillus, shake culture, until the bacillus enters logarithmic growth phase;S3.the aspergillus niger ball is washed and then poured into the LB culture medium containing bacillus obtained in S2, combined culture, form aspergillus niger and bacillus combination;S4.the aspergillus niger and bacillus combination is placed in contaminated water body, filtration centrifugation, remove nano selenium from water phase, and the aspergillus niger and bacillus combination is filtered out, realize repeated water purification.The present application can achieve substantial effect on selenium contaminated water quality treatment, and reduction efficiency is high, and the selenium tolerance is strong.

The invention provides a rapid degradation process for a degradable express green plastic package. The rapid degradation process comprises the following steps: 1, classifying raw materials, and then crushing and weighing the raw materials; 2, preparing culture soil: mixing vermiculite, base soil, water and nutrient substances to prepare the culture soil; 3, preparation of culture bacteria: proportioning aspergillus niger, Penicillium Polish and Bacillus thuringiensis according to a certain ratio, and adjusting the concentration; 4, uniformly mixing: feeding the crushed plastic, the culture soil and the culture bacteria according to the addition amount of the volume of the reaction container, and uniformly mixing; 5, the mixture is put into a reaction container, air is supplemented while stirring, the air is CO2-removed gas, the temperature in the reaction container is controlled to be 15-55 DEG C, the water content of a reaction mixture is controlled to be 40-60%, and the pH value is controlled to be 7.0-9.0. The method has the beneficial effects that the degradable plastic package is degraded by adopting the microbial flora and the culture soil, and the method has the advantages of low cost, simplicity in operation, low energy consumption, high degradation efficiency and the like. And the reaction conditions are mild, so that the process is more environment-friendly and has industrial feasibility.

The invention belongs to the field of microorganisms for degrading total petroleum hydrocarbons, and relates to a complex microbial inoculant and application thereof. The complex microbial inoculant comprises Aspergillus carneus strain and Bacillus thuringiensis strain, the preservation number of the Aspergillus carneus strain is CGMCC (China General Microbiological Culture Collection Center) No.41046, and the preservation number of the Bacillus thuringiensis is CGMCC No.29098. The invention further discloses a preparation method of the complex microbial inoculant. The complex microbial inoculant is stable in property, can rapidly grow in soil or underground water with total petroleum hydrocarbon as a unique carbon source, is excellent in total petroleum hydrocarbon degradation performance, and can be widely applied to remediation of soil or underground water containing total petroleum hydrocarbon.

The invention belongs to the technical field of microorganisms, and particularly relates to a lead-cadmium-resistant phosphorus-solubilizing fungus strain and application thereof. The fungus strain is named as PJC-1, and is classified and named as Aspergillus niger (Aspergillus niger); the fungus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 42129, and the fungus strain is preserved on July 15, 2025. The lead-cadmium-resistant phosphorus-solubilizing fungus strain obtained by the invention has strong tolerance to heavy metals lead (Pb) and cadmium (Cd), and the highest phosphorus-solubilizing capacity can reach 845.27 mg / L. The lead-cadmium-resistant phosphorus-solubilizing fungus strain and calcium phosphate are jointly added into the heavy metal contaminated soil, the effective state content of Pb and Cd in the soil can be effectively reduced, and the application prospect in the field of soil heavy metal pollution remediation is wide.