134 results about "Aspergillus zonatus" patented technology

The invention relates to the field of microorganisms, in particular to bacillus subtilis QNF2 and application of the bacillus subtilis QNF2 to prevention and control of fruit and vegetable diseases. The bacillus subtilis QNF2 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on August 26, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.31740. The bacillus subtilis QNF2 disclosed by the invention has the advantages that the bacillus subtilis QNF2 disclosed by the invention can be used for preparing the bacillus subtilis QNF2; according to the present invention, with the application of the bacillus subtilis QNF2, the growth of pathogenic bacteria such as Monilinia fucicola, Botryosphaeria dothidea, Alternaria alternata, Fusarium oxysporum, Aspergillus niger, Penicillium digitatum, Botrytis cinerea and the like can be significantly inhibited, and the occurrence of related fruit and vegetable diseases can be efficiently prevented and controlled, for example, the prevention and control effect of the volatile matter VOCs of the bacillus subtilis QNF2 on the peach brown rot caused by the Monilinia fucicola can be up to 98%; and the fruit quality, especially the fruit hardness, can be improved, and an important role in prolonging the storage period of the fruits is played.

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention discloses a nucleic acid product, a kit and a method for detecting aspergillus and application of the nucleic acid product, the kit and the method in detecting mildewed tobacco leaves. The nucleic acid product comprises a primer pair and a probe for detecting aspergillus, the primer pair comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2, and the nucleotide sequence of the probe is as shown in SEQ ID NO: 3. The nucleic acid product can specifically detect aspergillus, can realize rapid detection of tobacco mildew based on qPCR technology, has high sensitivity, simple operation, low cost and fast detection speed, can complete the whole detection process within several hours, does not need uncapping in amplification and detection processes, and avoids aerosol pollution caused by uncapping detection in common PCR.

The invention discloses a decay-promoting and carbon-retaining agent and a preparation method, application and a verification method thereof. The decay-promoting and carbon-retaining agent comprises a complex microbial inoculant and a nutrient preparation, the complex microbial inoculant comprises the following substances in parts by weight: 2 parts of aspergillus oryzae, 4 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 2 parts of lactic acid bacteria, 4 parts of Koxime saccharomycetes, 3 parts of acetobacter aceti, 4 parts of aspergillus candidus, 1 part of deuteromycetes and 1 part of arthrobacter; the nutrient preparation comprises the following substances in parts by weight: 5 parts of urea, 2 parts of diammonium phosphate and 1.3 parts of potassium sulfate. According to the method, the method for verifying the effect of mixed application of the straw and the decay-promoting and carbon-retaining agent to the soil is constructed, and the 13C isotope tracing technology is combined to verify the SOC immobilization effect of the decay-promoting and carbon-retaining agent on the soil with different textures, so that the situation that the decay-promoting and carbon-retaining agent with different components can achieve more effective SOC immobilization on the soil with the different textures is obtained, and straw resources are fully utilized.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention discloses Wickerhamomyces anomalus capable of producing volatile compounds and application of the Wickerhamomyces anomalus in prevention and treatment of aspergillus flavus, and belongs to the technical field of microorganisms. According to the invention, a strain of Wickhamomyces anomalus FP246 is obtained through screening, the strain of Wickhamomyces anomalus FP246 is preserved in the China Center for Type Culture Collection on July 31, 2024, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20241719. The Vickerhamomyces anomalus FP246 has an obvious antagonistic effect on aspergillus flavus, and can be used as an effective biological mildew-proof preparation for the dried chilies. The strain can inhibit aspergillus flavus hypha growth and spore germination by generating volatile compounds such as 2-methylbutyric acid, 3-methylbutyric acid and acetic acid. The method for spraying the dried chilies by utilizing the Vickerhamomyces anomalus FP246 is a novel biological control method, and has extremely high research and application values on the development of novel green bacteriostatic drugs.

The invention discloses an aspergillus cristatus strain capable of increasing gastrodin content and application of the aspergillus cristatus strain, and belongs to the technical field of solid state fermentation engineering. The Aspergillus cristatus strain ASE-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the Aspergillus cristatus strain ASE-4 is CCTCC (China Center for Type Culture Collection) NO: M20241392. By optimizing the fermentation method and modeling through the ANN-GA algorithm (R2 = 0.92), the gastrodin content (50% higher than that of an unfermented group) and the polysaccharide and total flavone content are remarkably improved. The strain and the fermentation method provided by the invention solve the problem that the gastrodin content is reduced in the prior art, and are suitable for development of high-added-value gastrodia elata products.

The invention relates to the technical field of in-vitro diagnosis, in particular to a primer probe combination for fungus PCR (polymerase chain reaction) detection. According to the invention, aiming at several detection targets of aspergillus, mucor, penicillium, blue marneffei and pneumocystis yarrowii, 18S rRNA, 5.8 S rRNA, 18S rRNA, ITS2 and a mitochondrial small subunit are respectively adopted as detection target regions for detection primer probe design, and different reaction systems are given for matching to complete automatic amplification. According to the primer probe combination and the kit for fungus PCR detection, the specificity for detecting different species of fungi is high, the detection system is stable, the detection limit is as low as 2 bacteria / mL, the joint detection design is beneficial to targeted coverage of fungal pathogens, and missing detection or mixed detection is effectively prevented.

The utility model discloses an aspergillus oryzae fermentation extraction device, which belongs to the technical field of fermentation devices and comprises a water tank, an electric heating box fixedly connected to the top end of the water tank and a mixing box fixedly connected to the top end of the electric heating box, a steam box is arranged above the mixing box, and a pressing block is arranged above the steam box. According to the utility model, the electric heating box, the mixing box, the steaming box and the pressing block are matched with one another, so that the processes of steaming, mixing and fermenting rice can be realized, the rice does not need to be transferred for many times, and the efficiency from steaming to fermenting of the rice is obviously improved; according to the rice steaming machine, when the rice is steamed, the steamed rice is driven to move upwards, the rice penetrates through a net plate under the downward pressing action of a pressing block, the rice can be cut up under the cutting action of the net plate, and meanwhile the mixing difficulty of the rice and aspergillus oryzae strains can be remarkably reduced under the mixing action of a comb plate.

The invention discloses a nucleic acid composition for detecting fungi as well as a kit and a detection method thereof, and relates to the technical field of molecular diagnosis. The embodiment of the invention provides a group of nucleic acid compositions, the compositions comprise primer pairs 1-5, the compositions can realize synchronous detection of aspergillus, cryptococcus, pneumocystis yarrowii and mucor on the basis of a real-time fluorescent quantitative PCR technology, and the nucleic acid compositions have the remarkable advantages of short detection time, simplicity and convenience in operation, high sensitivity, strong specificity and the like.

Described are immunogenic compositions comprising a non-naturally occurring pan-fungal Kex peptide, and methods of using such compositions for the treatment or prevention of infection and / or diseases associated with fungal pathogens (e.g., Pneumocystis, Aspergillus, Candida, or Cryptococcus) in the subject. Also provided are compositions and kits for detecting or quantifying the presence of antibodies directed against a non-naturally occurring pan-fungal Kex peptide in a subject.

The invention discloses a strain of Bacillus amyloliquefaciens JK-2, a cultivation method and an application thereof, and belongs to the field of microbial technology. The invention screens an antagonistic strain JK-2 from honeysuckle leaves, which has a good antagonistic effect on the pathogen Fusarium equisetifolia that causes honeysuckle diseases. The strain is identified as Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ), and tested its effective antagonistic components and safety. It has a significant inhibitory effect on the pathogen Botrytis cinerea that causes gray mold in strawberries and tomatoes, the pathogen Botrytis cinerea that causes pear ring rot, as well as Aspergillus niger and Penicillium, proving that strain JK‑2 has a broad spectrum of antibacterial activity. Therefore, Bacillus amyloliquefaciens JK‑2 has great potential as a microbial control agent to improve crop yield and quality.

The invention belongs to the technical field of microbial agents, and particularly relates to an Aspergillus heteromorphus AJF-Z1 strain and application thereof. The aspergillus heteromorpha is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, and the preservation number of the aspergillus heteromorpha is CGMCC No. 41922. The Aspergillus heteromorphus AJF-Z1 strain provided by the invention has an obvious promotion effect on soybean growth and nodulation condition improvement under a pot experiment condition, so that an efficient and stable technology for regulating and controlling synergistic nodulation of host soybean and rhizobium through exogenous fungi is constructed, and soybean root development, nodulation nitrogen fixation and stress resistance are regulated and controlled from multiple dimensions.

The present invention belongs to the technical field of Aspergillus niger genetic engineering, and specifically relates to an Aspergillus niger with high mycelial protein production, a bacterial agent, an application and a method for high mycelial protein production. An ΔABSU The mycelial protein content of the strain increased by about 107.3% compared with the wild type, from 27.49% to 47.00%. An ΔABSU The hyphae of the strain are completely dispersed and no longer aggregate into balls, which is conducive to the recovery and film-forming of hyphae protein. AnΔ ABSU The growth rate of the strain was significantly accelerated, and the biomass reached 16.5g / L, which was 113.1% higher than that of the wild type. This strain has broad application prospects in microbial protein production.

The invention discloses a modified chitosan-based antibacterial material as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The ciprofloxacin grafted quaternized chitosan is successfully synthesized by taking the chitosan as a raw material, and the water solubility and the antibacterial ability of the chitosan are effectively improved. The antibacterial diameters of the ciprofloxacin grafted quaternized chitosan prepared by the preparation method disclosed by the invention on escherichia coli and staphylococcus aureus are respectively 35.34 to 39.06 mm and 40.45 to 44.51 mm, and the minimum MIC (Minimum Inhibitory Concentration) and MBC (Minimum Mass Chitosan) can reach 0.0005 mg / mL. In addition, the antibacterial material has an obvious inhibition effect on colletotrichum gloeosporioides and aspergillus niger, and the highest antifungal rates respectively reach 53.81 + / -2.17% and 54.88 + / -1.3%. Compared with the raw materials, the antibacterial ability is greatly improved, and the grafted and modified composite material is environment-friendly and can be used for antibacterial food packaging materials.

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention belongs to the field of microbial technology, relates to the screening, identification and application of functional bacteria, and discloses a black Aspergillus and its fermentation method and application. The black Aspergillus provided by the invention is Aspergillus niger YXlfs22.3, and its deposit number is CGMCC No.40261. The black Aspergillus Aspergillus niger YXlfs22.3 bacterial body and / or fermentation liquid provided by the invention can effectively prevent and control aphids: after the fermentation liquid stock solution is applied to the leaves of melon plants for 5 days, the insect population reduction rate is 96.19%, and the corrected control effect is 95.88%; after the fermentation liquid diluted 10 times is applied to the leaves of watermelon plants for 48 hours, the insect population reduction rate is 90.01%, and the corrected control effect is 86.89%. The invention can replace traditional pesticides and be used for preventing and controlling plant aphid pests, and has the advantages of low preparation cost, no pollution to the environment, high cost performance, etc.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The application discloses an Aspergillus specific gene and a detection method and application thereof, and belongs to the technical field of biotechnology and medical molecular diagnosis. The nucleotide sequence comprises a full length or a partial continuous fragment selected from any one of sequences shown in SEQ ID NO. 1 to SEQ ID NO. 20, or a complementary sequence thereof. In the detection, nucleic acid is extracted from a sample to be detected; a primer pair is used to perform a nucleic acid amplification reaction on the extracted nucleic acid; the presence or quantity of an amplification product is detected, and a real-time Ct value is analyzed; a critical value is determined based on a test on a known negative sample library; and whether Aspergillus is detected is determined according to the Ct value and the critical value. The application shows high specificity, sensitivity and repeatability in clinical sample detection, and the detection period can be shortened to within 4 hours, thereby solving problems of long detection period, low sensitivity and cross reaction of existing Aspergillus detection methods.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The application relates to the technical field of biological medicine, in particular to a preparation method of anti-aspergillus niger siRNA and a complex preparation thereof, wherein the anti-aspergillus niger siRNA comprises siR266 for a PmrA gene and / or siR505 for a SarA gene, the sequence of a sense strand and an antisense strand of the siR266 is shown in SEQ ID NO: 3-4 respectively, and the sequence of a sense strand and an antisense strand of the siR505 is shown in SEQ ID NO: 7-8 respectively. Wherein, the complex preparation adopts protamine sulfate as a carrier, and forms a complex with the anti-aspergillus niger siRNA. The complex preparation prepared by the application can effectively inhibit aspergillus niger, and can be applied to food preservation, postharvest protection of fruits and vegetables, and mildew prevention of daily necessities and the like, and is suitable for developing corresponding anti-fungal products.

The invention discloses a fructus cinnamoii essential oil composition with a bacteriostatic effect as well as a preparation method and application thereof, and relates to the technical field of medicines. The invention discloses a fructus cinnamoii essential oil composition with a bacteriostatic effect, and the fructus cinnamoii essential oil composition with the bacteriostatic effect comprises the following components: 1-3.5 parts by mass of fructus cinnamoii essential oil, 1 part by mass of a wall material composed of gelatin and Arabic gum in a mass ratio of 0.4: 0.6-0.7: 0.3, and 0-4 parts by volume of a formaldehyde solution, the preparation method of the composition is simple, and the composition has a remarkable inhibiting effect on aspergillus flavus, penicillium expansum and fusarium oxysporum, and has a good application prospect. The invention can be used for preparing fungus inhibitors. The fructus cinnamoii essential oil microcapsule prepared from the fructus cinnamoii composition provided by the invention is stable in physicochemical property and remarkable in antibacterial effect, the preparation method is simple, and the fructus cinnamoii essential oil microcapsule has a wide market application prospect.

A method for producing a food using koji, the method comprising: a step for fermenting a mixture containing rice, aspergillus, and water; and a step for heating the solids contained in the mixture. Further, a food using yeast, which is dried and contains a fermented decomposition product of rice produced using aspergillus and inactivated aspergillus.

The application provides a chromium-contaminated laterite soil repairing agent and a repairing method thereof, and the method realizes effective repair of the laterite soil through the steps of pretreating the soil, applying the repairing agent I and applying an electric field, adding the repairing agent II for maintenance, and finally connecting biological strains.The method comprises the following steps: removing sundries, plowing the soil, applying the repairing agent I containing a reducing agent, citric acid and other components, and treating under specific electric field conditions.Subsequently, the repairing agent II, such as organic fertilizer, iron oxide, nano zeolite and chitosan derivative, is added.Finally, specific proportions of aspergillus, citrobacter freundii and psychrobacter are connected for biological repair.The method comprehensively considers physical, chemical and biological repair means, aims to efficiently remove chromium pollution in the soil, improve the soil organic carbon content of the laterite soil, and restore the ecological function of the soil.

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

The present invention belongs to the technical field of aspergillosis treatment, and specifically discloses the use of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone in the prevention and treatment of Aspergillus fumigatus infection, that is, using it as the sole active ingredient in the preparation of a drug for preventing or treating diseases caused by Aspergillus fumigatus infection, or for the preparation of an antibacterial and / or bactericidal product for Aspergillus fumigatus. The present invention first discovered that carbonyl cyanide-4-trifluoromethoxyphenylhydrazone has a significant inhibitory effect on both wild-type and drug-resistant strains of the human pathogenic fungus Aspergillus fumigatus, and can inhibit its colony growth, hyphal growth, and conidia formation, thereby providing a new therapeutic drug for Aspergillus fumigatus infection. At the same time, it solves the problem of antibiotic resistance that plagues the clinical treatment of Aspergillus fumigatus infection, and can circumvent the disadvantage that antibiotics are prone to drug resistance.

The invention discloses a method for treating dairy farm wastewater in a stepped manner based on synechocystis, aspergillus and green algae. According to the wastewater treatment method disclosed by the invention, aspergillus is used in the first step, so that organic substances in the wastewater of the dairy farm can be quickly converted; in the second step, synechocystis is used, the synechocystis is screened from wastewater of a biogas digester in a dairy farm, has high treatment capacity on high-concentration wastewater in the dairy farm and is named as synechocystis ZSL-NS1, and rapid increase of biomass can be achieved on the basis of the first two steps. Finally, the removal rate of pollutants in the dairy farm wastewater can reach 80% or above; the biomass yield is increased by 2.3 times. In addition, the synechocystis ZSL-NS1 can also produce biomass such as chlorophyll, protein, polysaccharide, grease and the like, the purpose of recycling is achieved while pollution is removed, and the synechocystis ZSL-NS1 has application value.