100 results about "Aspergillus zonatus" patented technology

The invention discloses a method for detecting aspergillus flavus and aflatoxin B1 of crops based on an infrared spectrum. The method comprises the steps of crop sample material collection, rice multi-strain mould infection, spectrum collection, crop sample aflatoxin B1 determination, spectrum pretreatment, correction model construction and model verification. The invention aims at detecting the content of aspergillus flavus infection and toxin B1 thereof in crops by combining a near-infrared spectrometer and a stoichiometric method. The method has the advantages of being simple and easy to implement, high in analysis speed, high in detection precision, low in analysis cost, free of any pollution to the environment and the like, samples do not need any pretreatment, and the method has important practical value for guaranteeing the quality safety of agricultural products and reducing the carcinogenic risk of people.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention discloses a nucleic acid product, a kit and a method for detecting aspergillus and application of the nucleic acid product, the kit and the method in detecting mildewed tobacco leaves. The nucleic acid product comprises a primer pair and a probe for detecting aspergillus, the primer pair comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2, and the nucleotide sequence of the probe is as shown in SEQ ID NO: 3. The nucleic acid product can specifically detect aspergillus, can realize rapid detection of tobacco mildew based on qPCR technology, has high sensitivity, simple operation, low cost and fast detection speed, can complete the whole detection process within several hours, does not need uncapping in amplification and detection processes, and avoids aerosol pollution caused by uncapping detection in common PCR.

The invention discloses Aspergillus sp. DLF18 and application thereof, and relates to the technical field of microorganisms, and the preservation number of the Aspergillus sp. DLF18 is CGMCC (China General Microbiological Culture Collection Center) NO.41737. The strain can effectively utilize rice straws and / or bagasse to produce humic acid, and the content of humic acid in each gram of fermentation supernatant after the rice straws are fermented is 16.5%. When the DLF18 fermentation product is applied to crops, the growth condition of the crops can be remarkably improved, and the humus content in soil is increased. Meanwhile, a metagenomics analysis result shows that compared with a control group, after the DLF18 is applied into the soil, the species beta diversity is remarkably changed, and the functional gene abundance and the inter-group difference are remarkably changed. Therefore, the DLF18 has a wide application prospect in the aspects of agricultural straw utilization and soil remediation.

The invention discloses an aspergillus cristatus strain capable of increasing gastrodin content and application of the aspergillus cristatus strain, and belongs to the technical field of solid state fermentation engineering. The Aspergillus cristatus strain ASE-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the Aspergillus cristatus strain ASE-4 is CCTCC (China Center for Type Culture Collection) NO: M20241392. By optimizing the fermentation method and modeling through the ANN-GA algorithm (R2 = 0.92), the gastrodin content (50% higher than that of an unfermented group) and the polysaccharide and total flavone content are remarkably improved. The strain and the fermentation method provided by the invention solve the problem that the gastrodin content is reduced in the prior art, and are suitable for development of high-added-value gastrodia elata products.

The utility model discloses an aspergillus oryzae fermentation extraction device, which belongs to the technical field of fermentation devices and comprises a water tank, an electric heating box fixedly connected to the top end of the water tank and a mixing box fixedly connected to the top end of the electric heating box, a steam box is arranged above the mixing box, and a pressing block is arranged above the steam box. According to the utility model, the electric heating box, the mixing box, the steaming box and the pressing block are matched with one another, so that the processes of steaming, mixing and fermenting rice can be realized, the rice does not need to be transferred for many times, and the efficiency from steaming to fermenting of the rice is obviously improved; according to the rice steaming machine, when the rice is steamed, the steamed rice is driven to move upwards, the rice penetrates through a net plate under the downward pressing action of a pressing block, the rice can be cut up under the cutting action of the net plate, and meanwhile the mixing difficulty of the rice and aspergillus oryzae strains can be remarkably reduced under the mixing action of a comb plate.

The invention discloses a nucleic acid composition for detecting fungi as well as a kit and a detection method thereof, and relates to the technical field of molecular diagnosis. The embodiment of the invention provides a group of nucleic acid compositions, the compositions comprise primer pairs 1-5, the compositions can realize synchronous detection of aspergillus, cryptococcus, pneumocystis yarrowii and mucor on the basis of a real-time fluorescent quantitative PCR technology, and the nucleic acid compositions have the remarkable advantages of short detection time, simplicity and convenience in operation, high sensitivity, strong specificity and the like.

Described are immunogenic compositions comprising a non-naturally occurring pan-fungal Kex peptide, and methods of using such compositions for the treatment or prevention of infection and / or diseases associated with fungal pathogens (e.g., Pneumocystis, Aspergillus, Candida, or Cryptococcus) in the subject. Also provided are compositions and kits for detecting or quantifying the presence of antibodies directed against a non-naturally occurring pan-fungal Kex peptide in a subject.

The invention belongs to the technical field of microbial agents, and particularly relates to an Aspergillus heteromorphus AJF-Z1 strain and application thereof. The aspergillus heteromorpha is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, and the preservation number of the aspergillus heteromorpha is CGMCC No. 41922. The Aspergillus heteromorphus AJF-Z1 strain provided by the invention has an obvious promotion effect on soybean growth and nodulation condition improvement under a pot experiment condition, so that an efficient and stable technology for regulating and controlling synergistic nodulation of host soybean and rhizobium through exogenous fungi is constructed, and soybean root development, nodulation nitrogen fixation and stress resistance are regulated and controlled from multiple dimensions.

The invention discloses a modified chitosan-based antibacterial material as well as a preparation method and application thereof, and belongs to the technical field of antibacterial materials. The ciprofloxacin grafted quaternized chitosan is successfully synthesized by taking the chitosan as a raw material, and the water solubility and the antibacterial ability of the chitosan are effectively improved. The antibacterial diameters of the ciprofloxacin grafted quaternized chitosan prepared by the preparation method disclosed by the invention on escherichia coli and staphylococcus aureus are respectively 35.34 to 39.06 mm and 40.45 to 44.51 mm, and the minimum MIC (Minimum Inhibitory Concentration) and MBC (Minimum Mass Chitosan) can reach 0.0005 mg / mL. In addition, the antibacterial material has an obvious inhibition effect on colletotrichum gloeosporioides and aspergillus niger, and the highest antifungal rates respectively reach 53.81 + / -2.17% and 54.88 + / -1.3%. Compared with the raw materials, the antibacterial ability is greatly improved, and the grafted and modified composite material is environment-friendly and can be used for antibacterial food packaging materials.

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention discloses a method for improving antioxidant activity of oil cakes through multi-strain fermentation combined with enzymolysis and application. The method comprises the following steps: adding a multi-strain mixed bacterium suspension into sterilized oil cakes, uniformly mixing, performing constant-temperature fermentation at 30 + / -2 DEG C, performing intermittent stirring in the fermentation process, and sequentially performing high-temperature sterilization and freeze-drying treatment after the fermentation is finished, the multi-strain mixed bacterium suspension is a mixed bacterium suspension of aspergillus niger, saccharomyces cerevisiae and bacillus subtilis, and the viable count ratio of the aspergillus niger to the saccharomyces cerevisiae to the bacillus subtilis in the multi-strain mixed bacterium suspension is (1-2): (1-2): (1-2). The application finds that a multi-strain composite fermentation combined enzymolysis technology can realize the synergistic interaction of fermentation and enzymolysis, obviously improves the antioxidant activity of the oil cake meal, and can be applied to preparation of cake meal feed.

The invention discloses a strain of Lysinibacillus bacilli B1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653, and the preservation number of the bacstein lysine bacillus B1 is GDMCC No: 66653. The bacstein lysine bacillus has an inhibition effect on penicillium expansum, aspergillus ochraceus, botrytis cinerea, escherichia coli, listeria monocytogenes, sclerotium rolfsii, root rot bacteria, ralstonia solanacearum and fusarium oxysporum. The strain can be used for preventing and treating southern blight and root rot of siraitia grosvenorii, banana fusarium wilt, mycosis of navel oranges and the like; the bacterial fertilizer can be applied to the fields of fruit preservation and bacterial fertilizer biocontrol of the plants; meanwhile, the strain can also be applied to the field of food preservation, is a bacterial strain with multiple functions and wide application fields, and has a good application prospect.

The application discloses an Aspergillus specific gene and a detection method and application thereof, and belongs to the technical field of biotechnology and medical molecular diagnosis. The nucleotide sequence comprises a full length or a partial continuous fragment selected from any one of sequences shown in SEQ ID NO. 1 to SEQ ID NO. 20, or a complementary sequence thereof. In the detection, nucleic acid is extracted from a sample to be detected; a primer pair is used to perform a nucleic acid amplification reaction on the extracted nucleic acid; the presence or quantity of an amplification product is detected, and a real-time Ct value is analyzed; a critical value is determined based on a test on a known negative sample library; and whether Aspergillus is detected is determined according to the Ct value and the critical value. The application shows high specificity, sensitivity and repeatability in clinical sample detection, and the detection period can be shortened to within 4 hours, thereby solving problems of long detection period, low sensitivity and cross reaction of existing Aspergillus detection methods.

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The application relates to the technical field of biological medicine, in particular to a preparation method of anti-aspergillus niger siRNA and a complex preparation thereof, wherein the anti-aspergillus niger siRNA comprises siR266 for a PmrA gene and / or siR505 for a SarA gene, the sequence of a sense strand and an antisense strand of the siR266 is shown in SEQ ID NO: 3-4 respectively, and the sequence of a sense strand and an antisense strand of the siR505 is shown in SEQ ID NO: 7-8 respectively. Wherein, the complex preparation adopts protamine sulfate as a carrier, and forms a complex with the anti-aspergillus niger siRNA. The complex preparation prepared by the application can effectively inhibit aspergillus niger, and can be applied to food preservation, postharvest protection of fruits and vegetables, and mildew prevention of daily necessities and the like, and is suitable for developing corresponding anti-fungal products.

The application provides a chromium-contaminated laterite soil repairing agent and a repairing method thereof, and the method realizes effective repair of the laterite soil through the steps of pretreating the soil, applying the repairing agent I and applying an electric field, adding the repairing agent II for maintenance, and finally connecting biological strains.The method comprises the following steps: removing sundries, plowing the soil, applying the repairing agent I containing a reducing agent, citric acid and other components, and treating under specific electric field conditions.Subsequently, the repairing agent II, such as organic fertilizer, iron oxide, nano zeolite and chitosan derivative, is added.Finally, specific proportions of aspergillus, citrobacter freundii and psychrobacter are connected for biological repair.The method comprehensively considers physical, chemical and biological repair means, aims to efficiently remove chromium pollution in the soil, improve the soil organic carbon content of the laterite soil, and restore the ecological function of the soil.

The invention discloses a calcium-based soil conditioner as well as a preparation method and application thereof, and relates to the technical field of soil improvement. The calcium-based soil conditioner comprises straw, calcium salt, nitrogen-phosphorus-potassium fertilizer and acid-resistant bacillus. Compared with the prior art, after the calcium-based soil conditioner provided by the invention is added into the soil, the abundance of Xanthobacraceae (xanthobacteriaceae) and Xanthomonadaceae (xanthomonas family) in the soil is obviously increased, and meanwhile, the abundance of Aspergillus flavus (aspergillus) and the abundance of Rhizopus arrhzizus (rhizopus arrhizus) in the soil are also obviously reduced.

The present invention belongs to the technical field of aspergillosis treatment, and specifically discloses the use of carbonyl cyanide-4-trifluoromethoxyphenylhydrazone in the prevention and treatment of Aspergillus fumigatus infection, that is, using it as the sole active ingredient in the preparation of a drug for preventing or treating diseases caused by Aspergillus fumigatus infection, or for the preparation of an antibacterial and / or bactericidal product for Aspergillus fumigatus. The present invention first discovered that carbonyl cyanide-4-trifluoromethoxyphenylhydrazone has a significant inhibitory effect on both wild-type and drug-resistant strains of the human pathogenic fungus Aspergillus fumigatus, and can inhibit its colony growth, hyphal growth, and conidia formation, thereby providing a new therapeutic drug for Aspergillus fumigatus infection. At the same time, it solves the problem of antibiotic resistance that plagues the clinical treatment of Aspergillus fumigatus infection, and can circumvent the disadvantage that antibiotics are prone to drug resistance.

The invention provides a bacterial strain for preventing and treating tobacco black shank, which is a new aspergillus strain Aspergillus candidus, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO.40802. The invention also provides a preparation method of the bacterial strain for preventing and treating tobacco black shank. The obtained MFZ2320 strain with biological activity on tobacco black shank can be applied to prevention and treatment of tobacco black shank on tobacco, the bacteriostatic activity of a crude extract is tested by adopting an agar diffusion method, and a result shows that when the concentration of a secondary metabolism crude extract of the marine fungus MFZ2320 strain is 10mg / mL, the bacteriostatic diameter on tobacco black shank reaches 16.67 mm, the inhibition rate is 37.04%, and the marine fungus MFZ2320 strain can be applied to prevention and treatment of tobacco black shank. The obvious antibacterial effect is shown.

The invention provides compounds which exhibit superior antifungal activity and are used for the treatment of fungal infections such as localized fungal infections and systemic fungal infections, caused by Trichophyton, Candida, Aspergillus and the like. In particular, the invention relates to an azole compound represented by the general formula (I)wherein X is a —N(R1)CO2R2 group wherein R1 is a hydrogen atom and the like, and R2 is a C1-6 alkyl group, etc., a —C(R3)═NOR4 group wherein R3 is a hydrogen atom and the like, and R4 is a C1-6 alkyl group, etc., or the like, Y is a halogen atom or the like, and Q is CH or a nitrogen atom, or a salt thereof.

This invention relates to the X178 strain, which inhibits fungal diseases in grains and removes mycotoxins, and its applications. The X178 strain belongs to the Nocardiaceae family (…). Nocardiopsis This fungus (sp.) is deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 34027. Specifically, it exhibits inhibitory effects against grain pathogenic fungi Aspergillus flavus, Aspergillus niger, and Fusarium graminearum, and can remove two mycotoxins: aflatoxin B1 (AFB1) and / or zearalenone.

The invention belongs to the field of biotechnology and fermentation engineering, and creates a group of probiotic flora by utilizing the mutual growth, symbiosis and antagonism of the probiotic flora. The probiotic group is composed of bacillus amyloliquefaciens (YR003, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) NO.32698), bacillus subtilis (YR002, the preservation number of which is CGMCC NO.32697), and bacillus siamensis (YR001, the preservation number of which is CGMCC NO.32696). The content ratio of bacillus amyloliquefaciens to bacillus subtilis to bacillus siamensis in the flora is 5: 3: 2. The flora can effectively degrade plant pollen allergen, pet allergen, dust mite allergen and insect excrement allergen and effectively inhibit mycospore allergen. Specifically, poplar pollen allergen, willow pollen allergen, phoenix tree pollen allergen, cypress pollen allergen, cat allergen, dog allergen, house dust mite allergen, dermatophagoides farinae allergen and cockroach allergen are effectively degraded; penicillium allergen, black mould allergen, cladosporium allergen and aspergillus allergen are effectively inhibited.

Disclosed are Aspergillus terreus ANU-301 strain (Accession Number: KACC49929), and ANU-301 strain (Accession Number: KACC49929) and a composition for controlling bacterial plant diseases using the culture filtrate. Since the strain or culture filtrate, according to the present disclosure, enhances the stress resistance of plants, it enables sustainable agriculture to eliminate or minimize the use of chemical pesticides.

The present invention belongs to the field of microbial technology, and particularly relates to a phosphate-solubilizing Aspergillus terreus and its application in drought-resistant probiotics; the Aspergillus terreus of the present invention is obtained by screening the rhizosphere soil of pepper, and the Aspergillus terreus CY-05 ( Aspergillus terreus var. fluffy ) is a growth-promoting microbial strain with disease resistance, high yield of terreus ketone and phosphate solubilization ability, and is an excellent strain with strong survival ability and multiple functions such as improving crop drought tolerance. The present invention also provides a probiotic agent obtained by solid-state fermentation of Aspergillus terreus CY-05. The application of this agent can increase the tolerance of crops to drought, improve the yield and survival ability of crops under drought stress conditions, promote the conversion and utilization of insoluble phosphorus, and prevent and control the occurrence of root soil-borne diseases. Strain CY-05 is an indigenous fungus in the rhizosphere of pepper. It is non-toxic and harmless to humans and animals, does not cause environmental pollution, and helps to solve the problems of water shortage and phosphorus deficiency faced by agricultural production in large semi-arid planting areas. It has important economic significance.

This invention discloses a method and kit for rapid detection of deeply infected Aspergillus. The method involves mixing the sample to be tested with a nucleic acid cleavage extract and heating at 60–65°C for 8–20 min, followed by heating at 93–98°C for 5–10 min to obtain a DNA extract. This DNA extract is then added to a PCR premix containing a composition of deeply infected Aspergillus to prepare a PCR reaction solution. The PCR reaction solution is subjected to the following PCR amplification reactions: denaturation is performed at 90–100°C for 0–5 s, followed by annealing and extension at 50–70°C for 0–5 s, repeated 35–50 times. This method not only provides a specific primer and probe composition capable of distinguishing between Aspergillus flavus, Aspergillus niger, and Aspergillus fumigatus, but also enables rapid detection of the sample from nucleic acid extraction to nucleic acid amplification, saving detection time.

This invention discloses a genetically engineered bacterium lacking the acetyltransferase gene asp12, its construction method, and its applications. This invention utilizes CRISPR / Cas9 to knock out the asp12 gene encoding acetyltransferase in Aspergillus terreus strain GZU-31-1, constructing the genetically engineered bacterium Δasp12. This invention found that compared to the wild-type Aspergillus terreus strain GZU-31-1, the genetically engineered bacterium Δasp12 exhibits increased accumulation and significantly higher yield of the compound Aspermeroterpene F, which possesses anti-inflammatory and anti-hepatic fibrosis activities. Based on the genetically engineered bacterium Δasp12, this invention also provides a method for preparing the compound Aspermeroterpene F, further improving the yield of the compound by optimizing the fermentation culture medium. This invention is beneficial for the development of anti-inflammatory and anti-hepatic fibrosis drugs.

The application discloses a biosynthesis gene cluster of a polyene macrolide natural product mandimycin, and a natural product and application of the biosynthesis gene cluster, wherein the nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO. 1, and the compound structural formula of the natural product mandimycin and mandimycin B is shown as formula I and formula II. The natural product mandimycin can target fungal cell membrane phospholipid molecules, especially phosphatidylinositol, cause important ion efflux in fungal cells, and lead to fungal cell death. The natural product mandimycin and mandimycin B have strong in-vivo and in-vitro antibacterial activity and a broad antibacterial spectrum on various WHO-published multiple drug-resistant fungal key pathogens, including candida, aspergillus, cryptococcus, mucor and fusarium.

The invention discloses aspergillus niger, a method for preparing graphene by parasitic graphite of the aspergillus niger and an industrial production method of the graphene, and belongs to the technical field of graphene preparation. According to the three-stage fermentation-centrifugal separation coupling system, graphene stripping is achieved through parasitism of aspergillus niger in graphite gaps, then filtering separation, ultrasonic stripping and impurity removal are conducted, graphene is obtained, and the invention further discloses an industrial production method of the three-stage fermentation-centrifugal separation coupling system for preparing graphene through aspergillus niger parasitism graphite. Aspergillus niger parasitism is utilized, the problems existing in preparation of graphene through a chemical oxidation-reduction method are reduced to a great extent, the preparation process is environmentally friendly, operation is easy, the action condition is mild and easy to control, and the method has the advantages that the fermentation condition is mild, the preparation process is clean and free of pollution, and a large amount of waste liquid is not generated; and the method has wide industrial application value and significance.