226 results about "Aspergillus zonatus" patented technology

The invention relates to preparation methods and uses of two dioxypiperazine compounds. The invention adopts aspergillus effuses H1-1 from root mud of Fujian mangrove forest to product novel structural dioxypiperazine compounds. Tests prove that: the compounds can be used as cellular breeding depressant or anti-tumor agent.

The invention relates to the field of bioengineering, in particular to a preparation method and an application of a microbial agent composition for enhancing secondary fermentation of a bean paste. The microbial agent composition consists of a saccharomycetes agent, an aspergillus oryzae agent and a pleospora agent; and the three microbial agents are prepared, and then the prepared microbial agents are mixed with the secondary-fermented bean paste, so as to promote the secondary fermentation of the bean paste. The whole process of the preparation method of the microbial agent composition provided by the invention is applicable to continuous industrial production; by-products (chili and radix platycodonis), from the production of Pixian bean paste, are comprehensively utilized; a production cycle can be shortened by 6 months, the content of amino nitrogen can be improved by 20% and the content of volatile aroma-producing components can be improved by more than 3 times (the contents of total ester, total acid and total aldehyde); by inoculating aflatoxin B1 with the saccharomycetes agent at a production peak (after being fermented for 30-60 days), an ester producing and aroma generating process can be enhanced, the metabolism of aspergillus flavus and partial aspergillus parasiticus can be competitively inhibited and the content of the aflatoxin B1 can be reduced, and the content of the aflatoxin B1 is lower than 0.5ppm, so that food safety is enhanced.

The invention provides a mulberry leaf feed as well as a processing method and application of the mulberry leaf feed. According to the mulberry leaf feed, dry mulberry leaf powder is artificially fermented by virtue of screened mixed bacteria of saccharomycetes, lactobacilli and aspergillus oryzae, so that the mouth feel of the mulberry leaf powder can be well improved, the ingestion capability of feed animals can be improved, and the apparent digestibility of the feed can be improved.

The invention provides a liriope spicate endogenous aspergillus fungus and application thereof to preparation of steroid saponin, and relates to the technical field of microorganisms. The fungus mainly solves the problems that due to the factors that the liriope spicate steroid saponin is mostly and directly from liriope spicate extracts, the liriope spicate steroid saponin content is low, the saponin extraction cost is high, and the like, the market delivery quantity of the liriope spicate steroid saponin is limited. The aspergillus fungus is separated from living body tuberous roots of liriope spicata var. prolifera, and belongs to aspergillus sp. through 18S rDNA sequence identification; the aspergillus fungus is preserved in CCTCC (China Center for Type Culture Collection); the preservation data is December 7, 2016; the preservation number is CCTCC No.M2016721. The liriope spicate produced by a liriope spicate GAP (Good Agricultural Practices) base (the liriope spicate source areain Oumao town, Xiangyang city in Hubei province ) is used; the liriope spicate steroid saponin is generated through liriope spicate endogenous fungus plant liquid fermentation. The endogenous fungus is an important microorganism for finding a new resource of the liriope spicate steroid saponin.

The invention discloses an aspergillus oryzae bacterial strain for producing feed compound enzyme with high yield and application of the aspergillus oryzae bacterial strain, and belongs to the technical field of biological fermentation. The bacterial strain for fermentation is specifically aspergillus oryzae DJ36, and is collected in the China General Microbiological Culture Collection Center with a collection number of CGMCC NO.13575. A polypeptide feed preparing process is implemented through a liquid fermentation technology in conjunction with an enzymatic hydrolysis technology, and specifically comprises the following steps: preparing a monospore bacterial suspension of the aspergillus oryzae; preparing a liquid bacterial strain; performing liquid fermentation with a triangular flask or a fermentation tank; performing enzymatic hydrolysis on impure protein with fermentation liquid; drying, smashing, packaging and the like. A functional small-molecular polypeptide is generated efficiently under the coaction of aspergillus oryzae fermentation and enzymatic hydrolysis at the later stage of fermentation, the advantages of a microbial fermentation technology and the enzymatic hydrolysis technology are combined, the use of an enzyme preparation is greatly reduced, the production cost of the polypeptide is lowered, and the industrialization process of the polypeptide is accelerated; moreover, a prepared feed additive has a relatively high nutrient value and disease resistance, and a wide application prospect.

The invention discloses high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance. The high-efficiency phosphate-solubilizing aspergillus japonicus with the heavy metal tolerance is classified and named as aspergillus japonicus, and is collected in China General Microbiological Culture Collection Center; the collection number is CGMCC No.7700. The invention also discloses the tolerance of the high-efficiency phosphate-solubilizing aspergillus japonicus to heavy metals. The high-efficiency phosphate-solubilizing aspergillus japonicus has an extremely good dissolving effect on insoluble phosphates, such as tricalcium phosphate, magnesium phosphate and aluminum phosphate in the situation of liquid shake flask culture; the dissolving effects on the three types of insoluble inorganic phosphorus reach 100 percent. The aspergillus japonicus has certain tolerance to the heavy metals after being incubated in liquid culture solutions which contain the heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>; particularly, the tolerated concentrations of the Pb<2+>, the Zn<2+>, the Cr<2+> and the Mn<2+> reach 2,000 mg/L respectively. Therefore, the high-efficiency phosphate-solubilizing aspergillus japonicus with heavy metal tolerance can provide excellent strain resources for the development of heavy metal-resistant high-efficiency phosphate-solubilizing bacterium agents.

The invention discloses a method for preparing questin by utilizing an ocean aspergillus flavipes HN4-13 bacterial strain. The method comprises the following steps: conducting separation and purification on antibacterial active substances generated through fermentation of the aspergillus flavipes HN4-13 bacterial strain according to silica-gel column chromatography, Sephadex LH-20 column chromatography, preparation of high performance liquid chromatography (PHPLC) and the like by taking vibrio harveyi as an indicator bacterium, so as to obtain a vibrio harveyi-preventing active compound HY2; indentifying the structure of the active compound HY2 according to spectra data such as ESI-MS, 1H-NMR and 13C-NMR, so as to determine that the active compound HY2 is questin. The questin is obtained through separation of a metabolic product of aspergillus flavipes HN4-13; experiments show that the questin can perform a certain inhibiting function of the pathogenic bacteria, such as vibrio harveyi, vibrio anguillarum, vibrio parahaemolyticus and vibrio cholerae, of aquatic products, and can be used for preparation of medicine preventing pathogenic vibrio of the aquatic products.

The invention discloses a method for preventing bamboo from mildewing by using aspergillus flavus. By utilizing the characteristic that in a given period, aspergillus flavus consumes more starch and reducing sugar than common mold and the physical mechanical performance of the bamboo does not drop obviously, the method comprises the following steps: inoculating the aspergillus flavus to the bamboo, so that the contents of starch and reducing sugar in the bamboo rapidly drop; and finally removing dark mold spots on the surface of the bamboo by bleaching, so as to recover the beautiful color of the bamboo surface. The technique is composed of aspergillus flavus culturing technique, aspergillus flavus inoculating technique, aspergillus flavus sterilizing technique and mold-infected bamboo bleaching technique. As proved in bamboo mold resistance experiments performed according to ASTM: D4445-03 (Standard Test Method for Fungicides for Controlling Sapstain and Mold on Unseasoned Lumber (Laboratory Method)): the bamboo is subjected to aspergillus flavus pre-infecting and then bleaching, the mold resistance of the bamboo is remarkably improved and no mold grows in an experiment period of 30 days.

The invention relates to the field of daily chemical products, in particular to a plant source antibacterial agent and a preparation method thereof. The plant source antibacterial agent comprises thefollowing components in parts by weight: 20-33 parts of a pulsatilla cernua extract, 14-36 parts of a Chinese prickly ash extract and 20-38 parts of a tillandsia usneoides extract. The plant source antibacterial agent provided by the invention has a very high property of inhibiting growth of bacillus subtilis, escherichia coli, staphylococcus aureus, candida albicans, pseudomonas aeruginosa, aspergillus niger and the like, and a preservative property as well, besides, the stability of the agent can be also very well improved at a high temperature. In addition, cosmetics can be kept gentle whenantibacterial effects and high-temperature stability are maintained, and the agent has the characteristics of being non-toxic and non-irritant to human bodies.

The invention relates to the field of wine making, and particularly relates to strains with high cellulase activity as well as a screening method and a use method of the strains. The strains with high cellulase activity are aspergillus. The screening method comprises the following steps of: A. enriching and cultivating bacteria through an inorganic salt culture medium; B. carrying out once or multiple-time inoculation on the bacteria enriched and cultivated on filter paper dipped with the inorganic salt culture medium; C. acquiring a cultured product at the fracture part of the filter paper, and carrying out once or multiple-time streaking on the cultured product on a carboxymethylcellulose culture medium plate, so as to obtain pure strains; D. cultivating the pure strains obtained from the step C in the inorganic salt culture medium and carrying out fibrinolysis on the cultivated pure strains; E. inoculating the pure strains with high fibrinolysis capacity in the step D to a steriled solid fermentation medium for cultivating; and F. extracting coarse enzyme liquid by adopting a water bath method, and carrying out enzyme activity test to obtain the strains with high cellulase activity. The strains and bread yeast are compounded for use and fermented by taking wine waste lees and brans as main materials. With the adoption of the strains with high cellulase activity, the fermentation rate of the wine waste lees can be improved.

The invention discloses a method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori 1042-2 which belongs to the Aspergillus and has the collection registration number of M209142 in the China Center for Type Culture Collection (CCTCC). The method for producing the cellulose and the polypeptide protein feed comprises the following steps: A, selecting the Aspergillus awamori 1042-2; B, authenticating the Aspergillus awamori 1042-2, C, culturing the Aspergillus awamori 1042-2 liquid; D, fermenting the solid material; and F, postprocessing. The solid culture which is obtained by inoculating the Aspergillus awamori 1042-2 onto the solid culture medium and fermenting the Aspergillus awamori 1042-2 and the solid culture medium is baked to dry, ground, sieved and subjected to the detection of the enzyme activity and the polypeptide protein content. The method for producing the cellulose and the polypeptide protein feed is feasible and easy, the produced powdered cellulose has the CMC enzyme activity of 31397.1-41826.7 U/g and the FPA enzyme activity of 1779.3-2212.1 U/g, has good stability and can be produced on a large scale, and the produced polypeptide protein feed contains more than 35 percent of protein. The Aspergillus awamori 1042-2 can be used for fermenting the palm kernel dregs, other dregs and the vegetable fiber and producing the biological feed, the biological fertilizer, the biological fuel and can be also used in the food industry, chemical industry and the pharmaceutical industry. The polypeptide protein feed produced by fermenting with the Aspergillus awamori 1042-2 can be used as the additive of the protein of various feed.

The invention discloses a microbial additive for alfalfa haylage, a preparation method and an application thereof. The additive is prepared from a bacterial suspension of aspergillusoryzae and an MRS liquid nutrient medium for streptococcus lactis according to the ratio of 50-70%: 50-30% in weight percent, wherein per milliliter of the bacterial suspension of the aspergillusoryzae at least contains 1.0*108 aspergillusoryzae spores, and per milliliter of the MRS liquid nutrient medium at least contains 4.0*108 cfu streptococcus lactis. The microbial additive quickens the initial fermentation rate of lactobacillus, enhances acid production capability, effectively inhibits the activity of saccharomycetes and other undesirable microorganism, strengths haylage effect and effectively promotes the production performance of cows, beef cattle, mutton sheep and swine. The strain adopted by the invention can be obtained easily, can be produced massively on application and has practicability.

The invention discloses an Aspergillus niger C2 of which the collection number is CGMCC NO.7511 and the collection date is July 5th, 2013. The Aspergillus niger C2 is separated from farmland soil in Hengshui Cicy Hebei Province in China, and has been collected at China General Microbiological Culture Collection Center; and the address is Institute of Microbiology in Chinese Academy of Sciences, Beichenxi Road Yard 3 No.3, Chaoyang District, Beijing City. The invention also discloses a microbial inoculum containing the Aspergillus niger C2. The microbial inoculum disclosed by the invention has obvious growth promotion effect on plants without being inhibited by the application of the fertilizer, can be used under the condition of normally using the fertilizer, and has important meanings for phosphate fertilizer resource saving for Chinese agricultural production, continuous yield increase of grains and protection of ecological environment of farmland.

The invention belongs to the field of biologics and in particular relates to a synchronous detection RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit for the yield of aflatoxin and the quantity of Nor-1 genetic transcription and a detection method of the kit. The invention establishes a synchronous RT-PCR detection method for synchronously detecting the yield of aflatoxin and the quantity of Nor-1 genetic transcription, and by using the method, the yield of the aflatoxin and the quantity of Nor-1 genetic transcription are in good linear relationship with results of HPLC (High Performance Liquid Chromatography) quantitative aflatoxin and Nanodrop quantitative Nor-1 genetic transcription, therefore, AFT-Nor-1 obtained by using the synchronous detection RT-PCR method has reliableand accurate ratio results which can be used as identification indexes for judging the toxin yields of aspergillus flavus strains, rapid and accurate identification evaluation on the toxin yields of the aspergillus flavus strains is achieved, and the kit has great significances for early warning and control on aflatoxin pollution.

The invention discloses a triazole antifungal compound and its preparation method and application, belongs to the field of pharmaceutical compounds, and specifically relates to a new triazole antifungal compound with the following general chemical structure formula. The preparation method of the compound of the present invention is economical, simple, mild and effective. The obtained compound has strong antibacterial activity against pathogenic fungi Candida albicans and Aspergillus, compared with clinical drugs fluconazole and itraconazole, it has the advantages of high efficiency and broad spectrum, and can be used to prepare new antifungal agents drug.

The invention belongs to the field of biotechnology microorganisms, and particularly relates to an Aspergillus clavatus-32 strain producing Lovastatin through liquid fermentation, application and a fermentation producing method. The Aspergillus clavatus-32 strain is high in Lovastatin yield and growing speed, large in sporulation quantity and simple to culture. Under the conditions that initial pH of a fermentation culture medium is 5.2 and culture temperature is 28 DEG C, after the liquid fermentation culture medium and fermentation conditions are optimized and the Ac-32 strain is fermented in a 5L fermentation tank for 110-140h, yield of Lovastatin can reach about 200-250mg/L.

The invention belongs to the technical field of herbicides, and discloses an aspergillus tubingensis L-27 strain of which a fermentation broth has herbicidal activity, the fermentation broth and an herbicide which are prepared from the strain, and an enrichment method and an identification method of herbicidal active components in the aspergillus tubingensis L-27 strain fermentation broth. By screening herbicidal activities of fermentation broths of 30 soil fungus strains, phenomena are found that inhibition rates of the aspergillus tubingensis L-27 strain fermentation broth on roots and stemsof amaranthus retroflexus, descurainia sophia and portulaca oleracea reach 100%; and regarding pharbitis purpurea, only stems grow weakly, and an inhibition rate still reaches 77.8%. A weak-base anion-exchange resin D301 is screened out to serve as an enrichment resin of the herbicidal active components, and after adsorption, growth inhibition rates of a residual liquid on roots and stems of wheat are only 1% and 7% respectively; and finally, a 100% ethanol solution of 0.1 mol/L HCL is determined to serve as a desorption agent of the active components enriched by the D301 resin. The herbicidal activity of the fermentation broth of the L-27 strain has potential development value.

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.

The invention discloses a composite microbial agent for efficiently degrading high-grease kitchen garbage and a preparation method of the composite microbial agent. The composite microbial agent provided by the invention is prepared from aspergillus oryzae powder, bacillus licheniformis powder, candida lipolytica powder, bacillus aceticus powder, bacillus coagulans powder and azotobacter chroococcum powder. By researching compounding of various microorganisms, when the compounding ratio of the aspergillus oryzae powder to the bacillus licheniformis powder to the candida lipolytica powder to bacillus aceticus to bacillus coagulans to the azotobacter chroococcum powder is 2:1:1.2:1.5:2:1 and the inoculation amount is 4%, the degradation rate of the kitchen garbage can reach 92% or above, main components (cellulose, grease, starch and protein) of the kitchen garbage can be effectively degraded, and the kitchen garbage can be fully fermented.

The invention relates to a method for producing fumagillin by aspergillus fumigatus. The method comprises the following steps: inoculating aspergillus fumigatus into a GMM solid medium to activate strains, wherein the pH value of the GMM medium is 5 to 7; preparing activated strain spores into spore suspension, and then inoculating the spore suspension into a CYA solid medium, a CXMA solid medium or an LMM liquid medium, wherein the pH values of the CYA solid medium and the CXMA solid medium are natural, and the pH value of the LMM liquid medium is adjusted to be 5 to 7, and carrying out culturing at the temperature of 25 to 37 DEG C; collecting cultured aspergillus fumigatus to extract fumagillin and fumagillin derivatives. According to the invention, carbon and nitrogen sources and trace elements of fumagillin which affect the growth of aspergillus fumigatus are aimed to be screened, and the concentration of corresponding trace elements is optimized to obtain higher yield of fumagillin.

The invention discloses aspergillus niger genetically engineered bacteria. The aspergillus niger genetically engineered bacteria are aspergillus niger in which glucan synthesis regulating gene VosA isknocked out. The invention also discloses a construction method of the above genetically engineered bacteria, and further discloses an application of the genetically engineered bacteria in the production of citric acid through immobilized fermentation. The aspergillus niger genetically engineered bacteria in which the glucan synthesis regulating gene VosA is knocked out are constructed, so that the hydrophobicity of the aspergillus niger during the production of the citric acid through immobilized fermentation is reduced, the yield of a biological film is reduced, a vector agglomeration phenomenon is reduced, the citric acid yield and sugar conversion efficiency are improved, and the aspergillus niger genetically engineered bacteria are suitable for industrial production.