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High-yield pectinase strain and application thereof

A technology for pectinase and pectinase preparation, which is applied in the field of microorganisms, can solve the problems of low juice yield, poor filtration efficiency, hindering the extraction, dissolution and stability of pigments and tannins, and achieves simple operation process and product enzyme activity. The effect of high and low production cost

Inactive Publication Date: 2017-09-12
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the large amount of pectin in grapes, it will lead to problems such as difficult clarification, low juice yield, and poor filtration efficiency.
And during the grape maceration process, the large amount of pectin present in the grape skin and pulp will also hinder the extraction, dissolution and stabilization of pigments and tannins, thus increasing the difficulty of winemaking

Method used

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Examples

Experimental program
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Embodiment Construction

[0014] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0015] In the implementation of the following example, unless otherwise specified, the sterilization is performed at high temperature (121°C, 20min).

[0016] The enrichment medium formula in the following implementation is as follows:

[0017] Pectin 2g·L -1 , ammonium sulfate 3g·L -1 , Potassium dihydrogen phosphate 1.4g·L -1 , Sodium hydrogen phosphate dodecahydrate 4.2g L -1 , Ferrous sulfate heptahydrate 0.01g L -1 , Magnesium sulfate heptahydrate 0.5g L -1 , Agar 20g·L -1 .

[0018] The basal medium formula in following implementation is as follows:

[0019] Orange peel powder 20g L -1 , peptone 5g·L -1 , Magnesium sulfate heptahydrate 1g·L -1 , sodium chloride 1g·L -1 , Dipotassium hydrogen phosphate trihydrate 1g·L -1 , Potassium dihydrogen phosphate 0.5g·L -1 .

[0020] The solid medium formula in the following im...

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PUM

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Abstract

The invention discloses a high-yield pectinase strain and application thereof. The high-yield pectinase strain is named aspergillus tubingensis Rn14-88A and is already preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on November 18th, 2016, and a preservation number of the high-yield pectinase strain is CGMCC No. 13184. The high-yield pectinase strain and the application have the advantage that the high-yield pectinase strain is screened from soil from vineyards.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-yield pectinase strain and its application. Background technique [0002] Pectinase is a general term for a compound enzyme that can decompose pectin. Pectinase mainly includes protopectinase, pectin methylesterase (PE), pectin lyase (Pectinlyase, PL), polygalacturonase (Polygalacturonases, PG), cellulase (cellulose) and so on. Protopectinase (protopectinase) can hydrolyze insoluble protopectin into water-soluble pectin, thereby cutting off the chemical bond between arabinose and polymethoxygalacturonic acid; pectin lyase (PL) can make long The pectin chains of chain molecules are broken to form pectin with many short-chain molecules; the main function of pectin methylesterase (PE) and polygalacturonyl esterase (PG) is to hydrolyze short-chain pectin into smaller Molecules to coagulate and separate by sedimentation; cellulase can reduce viscosity, increase ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/18C12N9/24C12N9/88C12R1/66
CPCC12N9/18C12N9/2402C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002C12N1/145C12R2001/66
Inventor 秦义黄丹梅宋育阳刘延琳
Owner NORTHWEST A & F UNIV
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