A kind of lysate and method for extracting meat nucleic acid

Abstract

The invention discloses a lysate and a method for extracting meat nucleic acid. The lysate is obtained by dissolving sodium chloride, sodium dodecylsulfonate, ethylphenyl polyethylene glycol and polyvinylpyrrolidone in water; the concentration of each component in the mixture is: sodium chloride: 0.2~ 1.5M, sodium dodecylsulfonate: 0.01~1g / ml, ethylphenyl polyethylene glycol: 0.01~0.5g / ml, polyvinylpyrrolidone: 0.01~0.1g / ml. The method is as follows: mix the minced meat sample with the lysate at normal temperature, and let it stand for a period of time; centrifuge again, and take the supernatant to obtain the nucleic acid extract. The lysate of the invention is low in cost and non-toxic, and the extraction method can realize rapid extraction of meat nucleic acid, the operation process is simple and easy, and can be applied to PCR or LAMP amplification system for detection.

Description

technical field [0001] The invention belongs to the technical field of food safety monitoring, and in particular relates to a lysate and a method for extracting meat nucleic acid. Background technique [0002] At present, in order to obtain economic benefits, many unscrupulous merchants in the market often replace high-priced meat with cheap meat, which seriously damages the rights and interests of consumers. Since the appearance of some similar types of meat (such as pork and beef, mutton and beef) is not very distinguishable, it is difficult for ordinary consumers to distinguish with the naked eye. In order to realize the identification of the type of meat, a common method is to use PCR or LAMP reaction to amplify and detect the unique nucleic acid sequence in the meat. However, before amplification, the nucleic acid of the meat needs to be extracted. [0003] There are many traditional meat nucleic acid extraction methods, such as phenol-chloroform method, CTAB method, ...

AI Technical Summary

Problems solved by technology

Some of these methods require heating, some operations take a long time, and there are many operating steps, the process is cumbersome, which greatly reduces the efficiency of nucleic acid extraction, which is not conducive to the rapid extraction and rapid detection of nucleic acids.

[0004] In order to speed up the extraction of nucleic acid from meat, there have been some reports on the extraction methods of nucleic acid from meat, such as using Tris-EDTA method, improved CTAB method, strong alkali method, urea method, etc. Although these methods simplify the operation compared with traditional detection methods Steps and procedures, but the operation is still time-consuming, and some reagents have certain toxicity, which is harmful to the human body

There are also many meat nucleic acid extraction kits (spin column type) on the market, but generally they need to be heated to lyse the meat for more than 1 hour, and the required reagents and operation steps are more, and the cost is higher

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