The invention discloses an underground water ecological
system health assessment method. An underground water
microorganism integrity index serves as a judgment index. The method includes the following steps that a microbial
community richness index is calculated, wherein microbial cells are collected,
genome DNA is extracted, PCR amplification is conducted, a target band is
cut for purification, and PCR products quantitatively obtained are subjected to paired-end sequencing; candidate biological parameters are determined, wherein a reference point and a damaged point are set, and parameters related to richness in the steps, parameters related to
community composition and parameters related to
interference resistance are selected as candidate parameters for calculating the biological integrity index; the value of the microbial integrity index is determined, wherein index value distribution
range analysis and judgment capacity analysis are conducted, and biological parameters are screened; health detection of an ecological
system is conducted by means of 95% quantile optimal value of the
microorganism integrity index of the reference point in the steps. The method has high sensitivity and can quickly, sensitively, accurately, comprehensively and objectively reflect health conditions of the underground water ecological
system.