Aspergillus tubingensis and application of aspergillus tubingensis in preparing yam diosgenin

Abstract

The invention discloses aspergillus tubingensis and an application of the aspergillus tubingensis in preparing yam diosgenin. The aspergillus tubingensis HG-57 has a preservation number of CCTCC (China Center for Type Culture Collection) M2016389. The method comprises the steps of inoculating an appropriate amount of aspergillus tubingensis hyphae to a PDA solid culture medium for activation, adding water to collect a spore suspension after spore production, inoculating the spore suspension to a seed culture medium for amplification to form a seed solution, inoculating the spore suspension or the amplified seed solution to a yellow ginger water extract, a yellow ginger alcohol extraction aqueous solution or a yellow ginger water suspension for aerobic fermentation for 1-7 days to form fermentation liquid, and separating and extracting the yam diosgenin product from the fermentation liquid. A yam diosgenin content is increased by 1.2-1.4 times, 1.1-1.4 times and 1.4-1.8 times compared with a content of the yam diosgenin obtained by directly adding acid into the system for hydrolysis. The strain can effectively convert yellow ginger saponin into the yam diosgenin; an acid hydrolysis conversion process in the traditional technology is substituted thoroughly; engineering amplification is very easy; and the strain has wide industrial application prospects.

Description

technical field [0001] The invention belongs to the field of microbiology and biotechnology, and more specifically relates to an Aspergillus tubingensis and its application in preparing turmeric saponin. Background technique [0002] Diosgenin, also known as saponin, is an important raw material for the production of steroid hormone drugs. More than 60% of the global steroid hormone drugs are produced synthetically or semi-synthetically with diosgenin as raw material. The traditional method of producing diosgenin is acid hydrolysis with strong acid, but this method has high pollution, high energy consumption and affects the water quality safety of the South-to-North Water Transfer Project. At the same time, in the process of acid hydrolysis, there are incomplete conversion of saponin and by-products are easily produced, which will affect the quality of saponin products. The current research generally believes that enzymatic hydrolysis and microbial transformation have good...

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Problems solved by technology

[0003] At present, some researchers have found that Fusarium exocytoma WX12 (CN 102703329 A) has the function of transforming and producing diosgenin, but it needs to add a series of inorganic salt components during the transformation process from the seed liquid to culture the bacteria and put it into the fermentation liquid. , the time spent is too long, the yield of diosgenin is low, and a considerable amount of water-soluble saponin is lost in the process of preparing turmeric saponin

Some researchers also found that Aspergillus oryzae (CN 101012474 B) can convert saponin into diosgenin, but there are many intermediate products and low conversion rate, so it is difficult to realize industrial application

Another researcher found that Aspergillus awamori (CN104232724 A) can convert turmeric saponin to produce diosgenin. Due to the binding effect of starch and cellulose in turmeric dry powder on saponin, the strain disclosed in the patent failed to convert part of the bound saponin, resulting in the production of diosgenin. low rate

There is research on the use of Lactobacillus acidophilus (CN 103497987 B) to transform turmeric saponin to produce diosgenin, which adopts the method of separating starch, cellulose and diosgenin and then intercepting diosgenin by nanofiltration. The comparison of the yield of saponin obtained by direct acid hydrolysis cannot correctly evaluate the transformation efficiency of the strain in the case of large differences in the quality of turmeric from different origins

Existing patents report that in the process of microbial transformation of turmeric saponin to produce saponin, the process is complicated, the form of converted saponin is single, the conversion efficiency is low, and the yield of saponin is low, so it is difficult to realize industrial application

[0004] Patent CN 103242418 A discloses a clean production process of turmeric saponin, using saccharification, membrane separation and ultrasonic extraction technologies to obtain saponin membrane concentrate and organic solvent saponin extraction concentrate, which can be more comprehensively used in industrialization level to obtain saponin, but in the process of converting saponin into saponin, acid hydrolysis is still used to catalyze the hydrolysis of saponin into saponin, which fails to achieve real green production

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