Benzopyrone dipolymer and extracting method and application thereof
A technology of benzopyrone dimer and extraction method, which is applied in the field of benzopyrone dimer preparation and achieves the effects of good cytotoxic activity and good antitumor activity
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[0035] Embodiments of the present invention are described in further detail below:
[0036] A benzopyrone dimer compound, the chemical structural formula of the compound:
[0037]
[0038] The extraction method of this compound is as follows:
[0039] (1) Carry out strain culture to cat feces endophyte Aspergillus tubingensis (Aspergillus tubingensis) DS37 in strain culture medium;
[0040] (2) In the fermentation medium, the bacterial classification obtained in step (1) is fermented and cultivated, and the fermentation product (9.2kg) is used as raw material to extract 8 times with petroleum ether / ethyl acetate / methanol, and the combined extracts are filtered , reclaim mixed solvent, concentrate under reduced pressure, obtain crude product extract 1500g;
[0041] (3) Put the crude product extract obtained in step (2) on a silica gel chromatographic column, and use petroleum ether with a volume gradient of 1:0:0, 0:1:0, 0:1:1, and 0:0:1 respectively / Ethyl acetate / methan...
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