Biological decolorizing method of melanoidin pigment

A melanin-like pigment and biological decolorization technology, applied in the field of biological wastewater treatment, can solve the problems of ineffective removal of pigments and high cost of removing pigments, and achieve excellent strains and biological treatment methods, good biological decolorization efficiency, and less secondary pollution. Effect

Active Publication Date: 2013-12-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of removing pigment by physical and chemical methods is relatively high, and microbial decolorization by means of microbial metabolism and pollutant reduction has the advantages of safety, effec

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Put the melanoid pigment into a 60ml / 250ml squeeze bottle and dilute to A 475 =3.5, add 30g / L sucrose, NaNO 3 1.5g / L, MgSO 4 .7H 2 O0.05g / L, KH 2 PO 4 1.0g / L, and adjust the pH to 4.0.

[0023] (2) Take out the preserved Aspergillus tubingensis strain and transfer it to the PDA test tube slant medium, put it into a biochemical incubator and keep the temperature at 30°C for 5 days. Wash the spores several times with sterilized 0.5% Tween 80 solution, add glass beads to oscillate and disperse, and filter to obtain the spore suspension, and measure the concentration of the spore suspension with a hemocytometer.

[0024] (3) The number of fixedly inoculated spores is 4.3×10 4 cells / ml, and calculate the required volume of spore suspension. Under sterile conditions, take a corresponding volume of spore suspension and inoculate it into the pre-prepared melanoid pigment liquid medium, and culture it at 39°C with shaking at 150rpm for 6 days. Take out the shaker flas...

Embodiment 2

[0026] (1) Put the melanoid pigment into a 50ml / 250ml squeeze bottle and dilute to A 475 =3.5, plus 5g / L fructose, NH 4 Cl2.0g / L, MgSO 4 .7H 2 O0.5g / L, KH 2 PO 4 0.5g / L, and adjust the pH to 7.0.

[0027] (2) Take out the preserved Aspergillus tubingensis strain and transfer it to the PDA test tube slant medium, put it into a biochemical incubator and keep the temperature at 29°C for 4 days. Wash the spores several times with 0.5% Tween80 solution that has been sterilized, add glass beads to shake and disperse, and filter to obtain the spore suspension, and measure the concentration of the spore suspension with a hemocytometer.

[0028] (3) The number of fixedly inoculated spores is 4.3×10 5 cells / ml, and calculate the required volume of spore suspension. Under sterile conditions, take the corresponding volume of spore suspension and inoculate it into the pre-prepared melanoid pigment liquid medium, and culture it with shaking at 30°C and 350rpm for 4 days, take out the...

Embodiment 3

[0030] (1) Put the melanoid pigment into a 50ml / 250ml squeeze bottle and dilute to A 475 =3.5, plus fructose 22g / L, peptone 1.5g / L, MgSO 4 .7H 2 O0.25g / L, KH 2 PO 4 1.5g / L, pH adjusted to 8.0.

[0031] (2) Take out the preserved Aspergillus tubingensis strain and transfer it to the PDA test tube slant medium, put it into a biochemical incubator and keep the temperature at 31°C for 3 days. Wash the spores several times with 0.5% Tween80 solution that has been sterilized, add glass beads to shake and disperse, and filter to obtain the spore suspension, and measure the concentration of the spore suspension with a hemocytometer.

[0032] (3) The number of fixedly inoculated spores is 4.3×10 4 per ml, calculate the required volume of spore suspension. Under sterile conditions, take a corresponding volume of spore suspension and inoculate it into the pre-prepared melanoid pigment liquid medium, culture at 40°C, shake at 150rpm for 6 days, take out the shaker flask, separate th...

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Abstract

The invention discloses a biological decolorizing method of melanoidin pigment. According to the technical scheme, aspergillus tubingensis is used for decolorizing the melanoidin pigment. The biological decolorizing method comprises the following steps of: culturing a strain with preservation number of CGMCC No. 7174 for 3 to 5 days at the temperature of 29-31 DEG C so as to prepare the aspergillus tubingensis, then inoculating aspergillus tubingensis spore suspension into a melanoidin pigment liquid medium, and carrying out shake cultivation for 4 to 10 days under the condition of controlling the temperature at 30-50 DEG C, the pH at 3.0-8.0 and the rotating speed at 50-350rpm for decolorizing reaction. The biological decolorizing method can be used for directly decolorizing the melanoidin pigment and has the advantages of safety, effectiveness, mild conditions, little secondary pollution, environmental protection, economy and the like; the decolorizing rate of the melanoidin pigment can reach up to 75%; a good strain and biological treatment method is provided for practical industrial wastewater treatment.

Description

technical field [0001] The invention belongs to the field of wastewater biological treatment, in particular to a method for biological decolorization of melanin-like pigments. Background technique [0002] Melanoid pigment is a kind of brown polymer formed by the Maillard reaction of sugar and amino acid. It has strong antioxidant and cytotoxicity when eaten. It is the main component of the high chroma of wastewater produced by the sugar industry and its extended industries such as molasses alcohol industry. It is resistant to temperature and light, and its color value will not decrease after being placed for a long time. The cost of removing pigment by physical and chemical methods is relatively high, and the microbial decolorization by means of microbial metabolism and pollutant reduction has the advantages of safety and effectiveness, mild conditions, less secondary pollution, environmental protection and economy, but the traditional activated sludge method This kind of...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 刘幽燕
Owner GUANGXI UNIV
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