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'Tabin' aspergillus capable of producing pectase in high yield, and application in production of solid-state fermentation

A technology of Aspergillus tubingensis and solid-state fermentation, which is applied in the direction of hydrolytic enzymes and fungi, and can solve the problems of increased activity of fermented enzymes

Inactive Publication Date: 2004-05-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Yet, for this reason so far, also do not have a kind of excellent microorganism strain that is used for the preparation of pectinase
At present, the microbial strains used in industry for the preparation of pectinase are mainly Aspergillus niger. Before 1993, there were many research reports on Aspergillus niger with high yield of pectinase, but the activity of fermentative enzymes increased limitedly, and the highest value was about 100u / g (pH5 .5 measured value), basically no breakthrough in the future

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 5ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid then, inoculate in the 50g seed culture medium (pH6.0, water content be 50%) that is made up of wheat bran (96%), ammonium sulfate (2.0%) and potassium dihydrogen phosphate (2.0%), After culturing at 30°C for 48 hours, insert 2 kg of solid medium (pH6.8) composed of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%) and orange peel powder (6.3%). , with a water content of 60%). After culturing at 28°C for 58 hours, the pectinase activity in the medium reached 122.4u / g.

Embodiment 2

[0072] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 10ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid 5ml then, inoculate in the 50g seed culture medium (pH6.0, water content is 50%) that is made up of wheat bran (96%), ammonium sulfate (2.0%) and potassium dihydrogen phosphate (2.0%), After culturing at 30°C for 48 hours, insert 2 kg of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%), orange peel powder (2.0%) and sugar beet pulp (4.0%). Solid medium (pH6.7, water content 60%). After culturing at 28°C for 60 hours, the pectinase activity in the medium reached 132.2u / g.

Embodiment 3

[0074] Take a slant strain of Aspergillus tubingensis Moss (Aspergillus tubingensis Moss) CGMCC 0826, add 5ml of sterile water, and wash the spores of Aspergillus tubingensis Moss. Draw spore liquid 3ml then, inoculate into the 20g that is made up of wheat bran (90%), ammonium sulfate (2.0%), potassium dihydrogen phosphate (2.0%), orange peel powder (2.0%) and sugar beet pulp (4.0%) In the solid medium (pH 6.8, water content 50%), after the medium was packed in a 250ml Erlenmeyer flask and cultured at 30°C for 62 hours, the pectinase activity in the medium reached 120.5u / g.

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PUM

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Abstract

A novel strain of Aspergillus tubingensis able to prepare the pectase with high output and its application in the solid-state fermentation of pectase are disclosed. The resultant pectase has high enzyme activity (more than 250 u / g at pH=2.5-4.2, or more than 120 u / g at pH=5.5).

Description

(1) Technical field [0001] The invention relates to a new strain of Aspergillus tubingensis Moss capable of high pectinase production and its application in pectinase solid-state fermentation. (2) Background technology [0002] In recent decades, due to the rapid increase of population and the continuous improvement of living standards, the demand for meat, poultry and eggs is also increasing year by year. Feed is the food of livestock and poultry. How to improve the utilization rate of feed and the growth performance of animals, how to protect the environment and ensure the sustainable, healthy and stable development of agriculture and animal husbandry have become important problems that human beings need to solve urgently. [0003] The feed compound enzyme produced by microbial fermentation is a kind of green feed additive that is non-toxic, harmless, and pollution-free. Adding an appropriate amount of enzyme preparations that match the feed ration can significantly improv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/24
Inventor 李德发陆文清朴香淑邢建军范石军张丽英胥学新龚利敏王春林
Owner CHINA AGRI UNIV
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