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Bacterial strain with antiviral activity and application thereof

An antiviral activity, strain technology, applied in the application, disinfectant, fungi and other directions, can solve the problems of difficult artificial synthesis, many chiral carbons, high synthesis cost, etc., and achieves suitable promotion and application, good compatibility, and production And the effect of simple extraction process

Inactive Publication Date: 2015-06-10
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some active substances that have a strong inhibitory effect on plant viruses have been isolated from plants, there are still few plant active substances that have been developed into anti-plant virus agents. The structure is complex, it is difficult to artificially synthesize or the synthesis cost is expensive, and the plants containing these active substances also have the disadvantages of long growth cycle and low yield.
For example, various bitternin compounds such as Brucea bruceipicrol, Brucea brucein B and Brucea brucein D isolated from Bructus javanica have strong inhibitory activity on plant viruses, but the chiral carbon of these compounds Many, complex structures, difficult to artificially synthesize, and the growth cycle of Brucea javanica is long and the yield is low, thus restricting its further development and application in agricultural production

Method used

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  • Bacterial strain with antiviral activity and application thereof
  • Bacterial strain with antiviral activity and application thereof
  • Bacterial strain with antiviral activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: A kind of isolating and purifying antiviral active substance malformin A from Aspergillus tubingensis FJBJ11 fermented liquid 1 Methods

[0017] (1) Inoculate the pure strain of Aspergillus tubingensis FJBJll on the PDA plate medium and culture at 28°C for 3 days; (2) Use a sterilized puncher to punch out 5 pieces of bacteria with a diameter of 4 mm on the edge of the colony of the medium Put the fungus cake in a 250 mL Erlenmeyer flask filled with 100 mL of PDB medium, shake it at 28 °C and 120 r / min for 4 days as the seed liquid; (3) inoculate the seed liquid with 5 % The amount of access consists of 10 g of peptone, 30 g of glucose, and 15 g of yeast powder , CaC1 2 1.5 g and 1000 mL of distilled water in a liquid medium, cultured on a shaker at 28 °C and 120 r / min for 10 days. (4) 50L of the filtrate after the fermentation broth was filtered was adsorbed by D101 macroporous resin column chromatography, first eluted with 4 column volumes of water,...

Embodiment 2

[0020] Embodiment 2: teratin A 1 One of the antiviral activity assays

[0021] (1) Test method (half-leaf dead spot method) Select healthy and uniformly grown heart leaf tobacco at the 6-8 leaf stage, sprinkle a small amount of 500-mesh quartz sand evenly on the leaves to be inoculated, and inoculate the left half leaf with teratin A 1 Mixed solution with virus (final concentration of virus is 10 μg / mL), the right half leaf is inoculated with virus solution prepared with distilled water (final concentration of virus is 10 μg / mL) as a control, rinse the inoculated leaves with distilled water 10 minutes after inoculation . Inoculate 4 leaves for each treatment, repeat 3 times, count the number of dead spots after 3 days, and calculate the inhibition rate according to the following formula.

[0022] Inhibition rate (%)=[(control number of dead spots - treatment number of dead spots) / control number of dead spots]×100%

[0023] (2) Test results The half-leaf blotch method was us...

Embodiment 3

[0026] Embodiment 3: teratin A 1 Antiviral Activity Determination Test 2

[0027] (1) Test method (leaf disk method) Select healthy common tobacco K 326 8 hours after inoculation with TMV, a disk with a diameter of about 15 mm was taken from the inoculated leaf avoiding the veins with a puncher, and floated in a certain concentration of teratin A dissolved in a small amount of DMSO (final concentration: 1%). 1 In the aqueous solution, the leaf discs inoculated with the virus and the healthy tobacco leaf discs not inoculated with the virus were respectively set as positive controls and negative controls floating in distilled water containing 1% DMSO. The leaf disc inoculated with the virus in the middle was used as the standard drug control, and 3 leaf discs were treated for each treatment. After 48 hours of treatment, the leaf discs were weighed, coated with 1:20 (W / V) carbonate buffer, and ground. The OD of each assay well was measured by indirect ELISA method 405 value. ...

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Abstract

The invention relates to a bacterial strain with an antiviral activity and an application thereof. The bacterial strain is aspergillus tubingensis FJBJ11, and has already been registered and preserved in the common microorganism center of the china microorganism strain preservation management council on January 26th, 2015, and the preservation serial number is CGMCC No.10435. The bacterial strain is used for preparing a virus disease prevention preparation. According to the bacterial strain, an antiviral activity substance is separated and purified from bacterial strain fermentation liquid and developed into a microorganism source antiviral agent, and therefore the bacterial stain has wide application prospects. The activity substance prepared through the bacterial strain is high in antiviral activity, free from pollution to the environment, capable of being used for preventing and treating plant viruses caused by tobacco mosaic viruses (TMV), and suitable for application and popularization.

Description

technical field [0001] The invention relates to a bacterial strain with antiviral activity and its application. Background technique [0002] tobacco mosaic virus ( Tobacco mosaic virus , TMV) has the characteristics of wide host range, great harm, and difficult control, and its control has always been the focus of scientific researchers. As the environmental and ecological problems caused by chemically synthesized pesticides have attracted increasing attention, the use of chemical pesticides has been increasingly restricted, while biological pesticides, mainly plant-derived pesticides and microbial-derived pesticides, have received more and more attention. . Although some active substances that have a strong inhibitory effect on plant viruses have been isolated from plants, there are still few plant active substances that have been developed into anti-plant virus agents. The structure is complex, it is difficult to artificially synthesize or the synthesis cost is expensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00C12R1/66
CPCA01N63/30C12N1/145C12R2001/66
Inventor 谭庆伟王福荣陈启建
Owner FUJIAN AGRI & FORESTRY UNIV
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