41 results about "Aspergillus coreanus" patented technology

A method for extracting the soluble calcium citrate by the manioc residue in a short period. The calcium citrate becomes more and more popular for the consumers as an ideal calcium supplement. The calcium citrate for sale in the market belongs to organic calcium, whose solubility is better than inorganic calcium and good for the absorption of human body to calcium, however, still belonging to the organic calcium with a low solubility, which restricts the absorption of human body to calcium. The inventive method mentioned above improves the absorption of the organic calcium and the mouthfeel by fermenting the manioc residue with Aspergillus niger. The produced organic calcium has a high solubility and tastes like lemon. The cost of the extracted soluble calcium citrate is equal to that of the citric acid with purity over 99.5%.

The invention relates to a strain capable of producing compound enzyme and a method of using the same to prepare compound enzyme for weaned piglets. The invention enables the problems that conventional feeds for weaned piglets are hard to digest and absorb and cause hypersensitivity to be effectively overcome. The strain provided in the invention is Aspergillus niger HKS11 which is preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 6027; and produced compound enzyme comprises protease, amylase and xylanase. The method of using the strain to prepare the compound enzyme comprises a step of activation of a culture, a step of acquisition of secondary seeds and a step of fermentation and culture in a solid fermentation cylinder. According to the invention, preparation cost is low, raw materials are rich and easily available, and energy saving and environmental protection are realized; when the produced compound enzyme for weaned piglets is added into a piglet feed, the utilization rate of the feed can be improved, productivity of the piglets is enhanced, and healthy growth of the piglets is promoted.

The invention relates to a preparation method of a long-chain alkane quaternization antibacterial agent, and a product and application of the antibacterial agent. Long-chain haloalkane is modified ona polymer richly containing tertiary amine; the carbon number on alkyl of the long-chain haloalkane is not smaller than 7. The prepared antibacterial agent has an excellent antibacterial effect on staphylococcus aureus, escherichia coli, candida albicans, helicobacter pylori, pseudomonas aeruginosa, aspergillus flavus and listeria monocytogenes; in the concentration dose with the antibacterial effect, cytotoxicity cannot be generated; the biological safety is realized. The preparation method is safe and simple and can be applied to industrial production; the development and application have development prospects.

The invention relates to a method for quantitatively and rapidly screening active materials for inhibiting the production of aflatoxin, which comprises the following steps: adding a microbial fermentation product, a plant extract, a natural product, or a chemical synthetic product with certain mass or concentration into a liquid culture medium, taking 200-500 microliters of obtained liquid, and adding the taken liquid into a micro-liquid incubator; by taking a blank nutrient solution as a contrast, inoculating spore suspension of 5-10 microliters of aspergillus parasiticus DM strains, culturing for 3-6 days at the temperature of 28 DEG C, visually inspecting the mycelial growth of the DM strains and the production of orange norsolorinic acid, and centrifuging the micro-liquid incubator with a visually inspected inhibition effect for 1 minute at a rotating speed of 1000 rpm; weighing the fresh weight of a mycelium, putting the mycelium into a methanol/sodium hydroxide solution, extracting norsolorinic acid therein for 30-40 minutes, measuring substances at 560 nm, and comparing the measured values with a light absorption value of an extracting solution; calculating a detoxification rate and a bacteriostasis rate; and selecting the substances with an inhibition rate of 90-100% as active materials for inhibiting the production of aflatoxin. The method disclosed by the invention can realize the trace, intuitive, quick, safe, and low-cost screening of active materials for efficiently inhibiting the production of aflatoxin.

The application discloses a water-treated potassium ferrate sterilizing agent and a preparation method thereof. The water-treated potassium ferrate sterilizing agent is prepared by weighing and uniformly mixing potassium permanganate, concentrated hydrochloric acid, potassium hydroxide, iron nitrate nonahydrate, manganese dioxide, potassium chlorate, a polymerization inhibitor, triethylene tetramine, formaldehyde, phosphorus trichloride, a dispersing agent, sodium hydroxide, phosphorous acid, glycine and polyaspartic acid according to parts by weight. The melting point of the product is 205 DEG C; the scale inhibition rate is 96.9-98.9%; the sterilizing rate to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium, aspergillus, saprophytic bacteria and salmonella is 99-100%; when the water-treated potassium ferrate sterilizing agent is placed at 50-60 DEG C for a month, the sterilizing rate is 97.5-99.5%; when the water-treated potassium ferrate sterilizing agent is placed at 40-50 DEG C for half a year, the sterilizing rate is 96-99%; when the water-treated potassium ferrate sterilizing agent is placed at the room temperature for 1-2 years, the sterilizing rate is 95-97%.

The invention discloses a non-oxidized type compound bactericide for water treatment and a preparation method thereof. The non-oxidized type compound bactericide is prepared by the steps of weighing glutaraldehyde, acrylic acid-2-acrylamide-2-methyl propane sulfonic acid copolymer, tetradecyl benzyl dimethyl ammonium chloride, etidronic acid, water, PBTCA, a polymerization inhibitor, sulfonic acid, polyol phosphonate ester, PESA, a dispersant, sodium tungstate, bitertanol, sodium benzoate and acrylic acid-hydroxy-propyl acrylate copolymer in parts by weight, and mixing uniformly. The product is 100% water-soluble, and has a sterilizing rate of 99.9-100% to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella; and the sterilizing rate is 98-100% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 97.9-99.9% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 97.5-99.5% after the bactericide is placed for 1-2 years at room temperature.

The application discloses an imidazoline biguanide sulfate bactericide for water treatment and a preparation method of the bactericide. The preparation method comprises the following steps: according to parts by weight, weighing the following materials: thiourea dioxide, dodecyl trimethyl ammonium bromide, hydrogen peroxide, brominated 1-butyl-2,3-dimethylimidazole, water, dodecylguanidine acetate, a polymerization inhibitor, sodium metasilicate pentahydrate, sulfuric acid, hexadecyl dimethyl benzyl ammonium chloride, a dispersing agent, dioctadecyl dimethyl ammonium chloride, imidazoline, triethylene tetramine and ethanol, and uniformly mixing the weighed materials; the scale inhibiting ratio of the product is 70-90%, and sterilizing rate on escherichia coli, staphylococcus aureus, candida albicans, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella is 97.9-99.9%; when the bactericide is kept at 50-60 DEG C for one month, the sterilizing rate is 97.5-99.5%, when the bactericide is kept at 40-50 DEG C for half an year, the sterilizing rate is 97-99%, and when the bactericide is kept at room temperature for 1-2 years, the sterilizing rate is 94-98%.

The invention provides an agricultural microbial enzyme, a bio-organic fertilizer and a preparation method, and belongs to the technical field of agricultural fertilizers. The agricultural microbial enzyme comprises the following raw materials in parts by weight: 10-50 parts of corn flour, 10-50 parts of wheat bran, 10-50 parts of rice husk, 0.01-1 part of a microbial agent and 20-50 parts of water. The microbial agent comprises bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus megatherium, bacillus mucilaginosus, bacillus laterosporus, aspergillus niger, saccharomycetes, lactic acid bacteria and actinomycetes. The agricultural microbial enzyme and the bio-organic fertilizer have higher stability and more active substances, can improve the utilization rate of the fertilizer, increase both production and income and improve soil, and have better pest and disease resistance effects.

The invention provides a biochar slow-release base fertilizer special for sweet potatoes. The biochar slow-release base fertilizer comprises a core layer and a coating layer coating the surface of the core layer. The core layer is formed by mixing of the following components by weight part: 60-80 of crop waste, 0.5-1.0 of Aspergillus oryzae, 6-8 of furfural residue, 2-4 of chili seed, 10-15 of clay, 15-20 of urea, 18-22 of calcium magnesium phosphate fertilizer, 30-40 of diammonium phosphate, 20-25 of potassium chloride, 2-4 of ferrous sulfate, 1-2 of chelated iron, 80-90 of biochar powder, and 0.2-0.4 of a fertilizer additive. The weight of the core layer accounts for 50%-60% of the total weight of the slow-release fertilizer. The coating layer is a mixture of biochar powder and a binder, and the weight of the coating layer accounts for 40%-50% of the total weight of the slow-release fertilizer. The nutrients of the fertilizer provided by the invention are slowly released under control, one-time fertilization can meet the demand of crops for fertilizer in the whole growth period, fertilizer consumption is reduced, and sweet potato yield is increased.

The invention relates to a bacterial strain with an antiviral activity and an application thereof. The bacterial strain is aspergillus tubingensis FJBJ11, and has already been registered and preserved in the common microorganism center of the china microorganism strain preservation management council on January 26th, 2015, and the preservation serial number is CGMCC No.10435. The bacterial strain is used for preparing a virus disease prevention preparation. According to the bacterial strain, an antiviral activity substance is separated and purified from bacterial strain fermentation liquid and developed into a microorganism source antiviral agent, and therefore the bacterial stain has wide application prospects. The activity substance prepared through the bacterial strain is high in antiviral activity, free from pollution to the environment, capable of being used for preventing and treating plant viruses caused by tobacco mosaic viruses (TMV), and suitable for application and popularization.

The invention relates to a preparation method of a plastic long-acting mildew preventive, which comprises the following steps: pulverizing a mildew preventive active matter, and screening with a 80-200-mesh sieve; respectively crushing polycarbonate, a plasticizer, an antioxidant, a slow-release framework material, an inert auxiliary agent and a heat stabilizer, and sieving with an 80-200-mesh sieve; respectively mixing the mildew preventive active matter with the polycarbonate, the plasticizer, the antioxidant, the slow-release framework material, the inert auxiliary agent and the heat stabilizer according to a ratio, and dissolving the mixture into trichloromethane and/or ethanol; and mixing the mixture with a plastic polymer according to a ratio of 1: 20-100 at a temperature of 180-220DEG C, drying, and crushing to prepare the plastic long-acting mildew preventive. The mildewpreventive disclosed by the invention has a very good sterilization effect on common aspergillus niger, escherichia coli, mucor and candida, and the mildew preventive active matter is coated with the slow-release framework material, so that the sterilization lasting time is longer.

Belonging to the technical field of pharmaceutical chemistry, the invention discloses a naphthalimide benzimidazole compound, a preparation method and application thereof. The compound has strong in-vitro antimicrobial activity, especially shows very high inhibitory activity to staphylococcus aureus, methicillin-resistant staphylococcus aureus, bacillus subtilis, micrococcus luteus and other gram-positive bacteria, escherichia coli, proteus, pseudomonas aeruginosa, salmonella typhi and other gram-negative bacteria, candida utilis, aspergillus flavus, saccharomyces cerevisiae, candida albicans,candida mycoderma bacteria and other fungi, can be used for preparation of antibacterial and/or antifungal drugs, and is conducive to solving increasingly serious drug resistance, stubborn pathogenicmicroorganisms, newly emerging harmful microorganisms and other clinical treatment problems.

The invention discloses a water treatment ternary copolymerization bactericide and a preparation method thereof. The preparation method comprises the following steps: weighing glutaraldehyde, AMPS, crylic acid, isopropanol, water, sodium hydroxide, a polymerization inhibitor, sodium hypophosphite, maleic anhydride, ammonium persulfate, a dispersing agent, phosphorus trichloride, N,N-dimethylformamide, L-aspartic acid and glutamic acid according to parts by weight, and uniformly mixing the materials to prepare the bactericide. The scale inhibiting ratio of the bactericide is 85.5-98.5%; the density is 1.15-1.35 g/cm<2>; after the bactericide is stored at 50-60 DEG C for one month, the bactericidal rate is 98.5-99.5%; after the bactericide is stored at 40-50 DEG C for half a year, the bactericidal rate is 97-99%; after the bactericide is stored at the room temperature for 1-2 years, the bactericidal rate is 96-98%; when the bactericide is applied to escherichia coli, staphylococcus aureus, streptococcus albus, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella, the bactericidal rate is 98.8-99.8%.

The invention provides a method for detecting food-borne pathogenic bacteria in a microecological active bacterial preparation through multiplex PCR. For the method provided by the invention, the multiplex PCR technology is mainly adopted, and the method adopts the PCR reaction in which multiple specific primers are added in the same reaction system, and multiple nucleic acid fragments are simultaneously amplified. According to the method, the specific primers of seven common food-borne pathogenic bacteria including salmonella, escherichia coli, bacillus pumilus, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa and aspergillus niger are designed, seven target bands are simultaneously amplified in the same system, meanwhile, the specificity among each other is good, the limit of detection of the method achieves 200fg/[mu]l (template concentration), the corresponding concentration of bacterium is 120CFU/mL, the standard of the food safety can be achieved, the common pathogenic bacteria can cause the pathogenic effect when the concentration is 1000CFU/mL, therefore, the method is high in sensitivity, and is rapid, simple and convenient, and the cost is very low.

The invention discloses a water treatment tertiary aminating resin bactericide and a preparation method thereof. The water treatment tertiary aminating resin bactericide can be prepared by matching and weighing benzene, micrococcus chlorinus, dimethylamine, hydrochloric acid, water, ethanol, a polymerization inhibitor, alkali, isopropanol, chlorotetradecane, a dispersing agent, chloroacetic acid, bisphenol-A, benzotriazole and PBTCA (phosphono butane-1,2,4-tricarboxylicacid) in parts by weight, uniformly mixing the raw materials and carrying out vacuum drying; and the water treatment tertiary aminating resin bactericide has a sterilizing rate of 98 to 100 percent on escherichia coli, staphylococcus aureus, canidia albicans, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella, has carrier content of 1 to 1.08mmol/g, has a corrosion rate of 0.0001 to 0.0021mm/a for tin brass, has a scale inhibition rate of 96 to 98 percent , has the sterilizing rate of 97.95 to 99.95 percent after being placed for one month at a temperature of 50 to 60 DEG C, has the sterilizing rate of 97.9 to 99.9 percent after being placed for half a year at a temperature of 40 to 50 DEG C, and has a sterilizing rate of 95 to 99 percent after being placed for 1 to 2 years at the room temperature.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses a gliotoxin 6-aromatic ring carboxylic ester compound (general formula I) which is designed and synthesized by taking a secondary metabolite gliotoxin of aspergillus fumigatus as a mother nucleus, and a preparation method thereof. The preparation method of the gliotoxin 6-aromatic ring carboxylate compound comprises the following steps: taking gliotoxin as an initial raw material, and carrying out esterification reaction on 6-site hydroxyl of the gliotoxin and aromatic ring or aromatic heterocyclic carboxylic acid on the premise of not damaging an active center disulfide bond of the gliotoxin, so as to obtain the gliotoxin 6-aromatic ring carboxylate compound. The inhibition activity of the series of compounds on histone lysine demethylase (LSD1) is obviously superior to that of maternal gliotoxin, and the series of compounds can be used for preparing anti-tumor drugs and can be applied to clinical treatment of diseases such as esophageal cancer, gastric cancer, lung cancer, colorectal cancer and breast cancer. The compound has a general formula I shown in the specification.

The invention discloses an animal and plant waste decomposition complex microbial inoculant and a bio-organic fertilizer prepared from the same, and belongs to the field of bio-organic fertilizers. The microbial inoculant comprises bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae, trichoderma reesei and aspergillus niger, and by adding the microbial inoculant into animal and plantwaste decomposition fermentation, decomposition of the organic fertilizer can be accelerated, and the fermentation time can be shortened. The animal and plant waste decomposition complex microbial inoculant disclosed by the invention can be quickly propagated in decomposition production activities of the organic fertilizers, so that cellulose, protein and other substances in animal and plant wastes are degraded and decomposed, harmful substances are absorbed or decomposed, odor substances are absorbed or neutralized to achieve a deodorization effect, a certain biological prevention and control function is achieved, and soil physicochemical properties are improved.

The invention relates to the field of freckle lightening cosmetics, in particular to a garlicin freckle lightening cosmetic and a preparation method thereof. The garlicin freckle lightening cosmetic comprises the preparation raw materials of A components, B components and C components, wherein the A components comprise the preparation raw materials in parts by weight of 2-5 parts of an emollient and 0.1-1 parts of a surfactant, the B components comprise the preparation raw materials of 80-90 parts of water, 7-10 parts of a skin-protecting agent, 0.05-0.15 part of a chelating agent, and 0.02-0.1 part of a pH regulation agent, and the C components comprise the preparation raw materials of 0.1-0.3 part of a preservative and 0.1-0.3 part of garlicin. The provided garlicin freckle lightening cosmetic is safe and free from stimulation, after the garlicin freckle lightening cosmetic is used for 5 weeks, the freckle lightening rate can achieve 84% or above, after the use of the garlicin freckle lightening cosmetic is stopped for 8 weeks, freckles do not recur, and besides, the garlicin freckle lightening cosmetic can also effectively resist bacteria, escherichia coli, candida albicans, aspergillus fumigatus, amebic protozoa and trichomonas vaginalis, so that people can prevent infection in daily life.

The invention discloses a primer composition for detecting pathogens of sepsis as well as a nucleic acid detection kit and a detection method of the primer composition. The invention discloses a primer composition for detecting pathogens of sepsis. The kit comprises a staphylococcus aureus primer group, a klebsiella pneumoniae primer group, an escherichia coli primer group, an enterococcus faecalis primer group, an acinetobacter baumannii primer group, a pseudomonas aeruginosa primer group, a stenotrophomonas maltophilia primer group, a streptococcus pneumoniae primer group, a neisseria meningitidis primer group and an aspergillus primer group. The primer group is at least one of an enterobacter cloacae primer group and a group B streptococcus primer group. According to the primer group for detecting the sepsis pathogens, the nucleic acid detection kit containing the primer group and the detection method provided by the invention, various sepsis pathogens can be rapidly and accurately detected, so that the defect that the existing sepsis pathogen detection technology is time-consuming and labor-consuming is overcome, the detection sensitivity and specificity are improved, the labor intensity is reduced, and the detection efficiency is improved. The detection period is shortened.