10 results about "Aspergillus coreanus" patented technology

The invention relates to a polypeptide for treating rhinitis as well as a preparation method and application thereof, and belongs to the technical field of biology. Natural active small molecule polypeptides are extracted from white tea plants, and the polypeptides are safe and non-toxic to cells and have remarkable bacteriostasis and sterilization effects on rhinitis pathogenic bacteria such as staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger; in addition, in a guinea pig in-vivo test, symptoms of allergic rhinitis and inflammatory factors such as IgE, IL6 and IFN-gamma can be remarkably improved, and the treatment effect is superior to or equivalent to that of a positive control group. The white tea polypeptide disclosed by the invention has a remarkable effect of treating rhinitis, provides a novel, safe and efficient treatment choice for rhinitis patients, and has important scientific value and clinical application prospect.

The invention belongs to the technical field of biological detection, and particularly relates to an aspergillus fumigatus monoclonal antibody combination and application thereof. The monoclonal antibodies specifically comprise murine monoclonal antibodies 3D2 and 1F10 for recognizing galactomannan antigens. The monoclonal antibody 3D2 is used as a coating antibody, the monoclonal 1F10 is used as a detection antibody, a double-antibody sandwich ELISA system is formed, and the detection sensitivity is remarkably improved by combining a biotin-avidin signal amplification technology. The invention also provides amino acid sequences and coding nucleotide sequences of heavy chain and light chain variable regions of the antibody, which can be used for development of detection tools such as reagents, kits, test strips or antibody chips. Experimental verification shows that the detection method shows good specificity and repeatability in clinical samples and has important application prospects.

The invention discloses an application of fermented sweet potato residues in a penaeus vannamei feed, which is characterized in that the sweet potato residues are subjected to enzymolysis and then inoculated with microorganisms for fermentation to obtain a fermented product, and then the fermented product is used for replacing part of fish meal to produce the penaeus vannamei feed. The fermentation product is prepared by the following steps: crushing sweet potato residues, uniformly mixing the crushed sweet potato residues with water to obtain a mixture, then adding amylase into the mixture for enzymolysis, then adding alkaline protease for enzymolysis, then adding urea, ammonium sulfate and a complex microbial inoculant containing candida utilis, candida lipolytica, aspergillus niger and lactobacillus bulgaricus, and mixing, thereby obtaining the fermentation product. Uniformly mixing and fermenting to obtain a fermented product. The waste sweet potato residues are subjected to enzymolysis and fermentation to prepare the fermentation product, and the fermentation product is used for replacing part of fish meal to produce the penaeus vannamei boone feed, so that the feed cost can be reduced, and the obtained feed can improve the growth performance, digestive enzyme activity and immune function of penaeus vannamei boone.

The invention discloses an enzyme preparation for livestock feed as well as a preparation method and application thereof, belongs to the technical field of agricultural microbial enzymes, and particularly relates to a preparation method of the enzyme preparation for livestock feed, which comprises the following steps: carrying out shaking table fermentation culture on a bacillus licheniformis solution to obtain a bacillus licheniformis fermentation mixed solution, centrifuging, and taking the supernatant to obtain a beta-mannase solution; performing fermentation culture on the bacillus velezensis bacterial liquid to obtain bacillus velezensis fermentation mixed liquid, performing centrifugation, and taking supernatant to obtain cellulase liquid; compounding aspergillus niger liquid, performing fermentation culture to obtain aspergillus niger liquid fermentation mixed liquid, and performing centrifugation to obtain compound enzyme liquid; mixing the beta-mannase liquid, the cellulase liquid and the compound enzyme liquid, and performing freeze drying treatment to obtain mixed enzyme powder; and mixing the mixed enzyme powder with momordica grosvenori glucoside to obtain the enzyme preparation for the livestock feed. The compound enzyme liquid is prepared by selecting specific strains for fermentation, the effect of improving the feed utilization rate of the prepared enzyme preparation is improved, and growth and development of livestock can be effectively promoted.

The invention belongs to the technical field of sweet potato processing, and particularly relates to a method for preventing roast sweet potatoes from spoilage, which comprises the following steps: cleaning harvested sweet potatoes, and soaking the cleaned sweet potatoes in a preservative solution for 30-60 minutes; the formula of each liter of the preservative solution is as follows: 2 g to 5 g of calcium beta-hydroxy-beta-methyl butyrate, 0.01 g to 0.03 g of potassium sorbate, 1 g to 2 g of L-methionine and water as a solvent; draining water after soaking, baking until the sweet potatoes are cooked, cooling to room temperature, spraying preservative liquid on the surfaces of the baked sweet potatoes, and drying to obtain the baked sweet potatoes; the spoilage is caused by reproduction of microorganisms, and the microorganisms comprise bacteria and / or fungi; the bacteria are at least one of staphylococcus aureus, bacillus subtilis, pseudomonas, Erwinia and lactic acid bacteria; the fungi are at least one of penicillium, aspergillus and mucor. The method provided by the invention can avoid the generation of microbial drug resistance, and can prevent the roast sweet potatoes from spoilage for a long time.

The present application relates to a kind of polypeptide for treating rhinitis, preparation method and use, belong to biotechnology field.The present application extracts natural active small molecule polypeptide from white tea plant, the polypeptide is safe and nontoxic to cell, and has significant bacteriostatic and bactericidal effect to rhinitis pathogenic bacteria such as staphylococcus aureus, pseudomonas aeruginosa, candida albicans and aspergillus niger;In addition, in guinea pig in-vivo test, the symptoms and IgE, IL6 and IFN-γ etc. of allergic rhinitis can be significantly improved inflammatory factors, and treatment effect is better than positive control group or comparable with positive control group.The white tea polypeptide of the present application has significant effect of treating rhinitis, provides a new type, safe, efficient treatment choice for rhinitis patient, has important scientific value and clinical application prospect.

The invention discloses a preparation method of monascus red pigment and the prepared monascus red pigment, and relates to the field of food additives. The preparation method of the monascus red pigment comprises the following steps: S1, inoculating a monascus strain into a basic culture medium to carry out batch propagation operation; s2, after the bacterial colony in the step S1 is mature, carrying out centrifugal separation to take solid residues, adding 3-5 times of alcohol with the volume concentration of 95% or above, stirring, then carrying out solid-liquid separation to take liquid, and concentrating at 45-55 DEG C to obtain a concentrated solution; s3, a protective agent is added into the concentrated solution obtained in the step S2, after high-speed uniform stirring is conducted, cooling and curing are conducted, and the protective agent is at least one of maltodextrin, Arabic gum and a sodium alginate water solution; and S4, performing freeze drying on the cooled and cured product in the step S3 to obtain the monascus red pigment. The monascus red pigment prepared by the method has the advantages of high yield, high thermal stability and the like.

The present invention relates to an aromatic fused-ring imine compound and its preparation method and application. The aromatic fused-ring imine compound has a rigid planar structure and multiple hydrogen bond acceptors and donors. Its rigid planar structure can produce a π-π stacking effect with aromatic amino acid residues exposed to the solution in the chitinase binding pocket. Its hydrogen bond acceptors (such as carbonyl oxygen atoms) and hydrogen bond donors can produce direct or water-mediated hydrogen bonds with amino acids in the binding pocket, thereby producing an inhibitory effect on chitinase. Through evaluation and research on its inhibitory effect and selectivity, it is known that the aromatic fused-ring imine compound exhibits inhibitory activity on chitinases from Asian corn borer, humans, Caenorhabditis elegans, Brugia malayi, Aspergillus fumigatus, and Serratia marcescens, and has a wide range of application scenarios in the fields of biology and biochemistry.

The invention discloses a microorganism bacterium brick for promoting plant growth and a preparation method and application thereof, the microorganism bacterium brick is composed of vinasse obtained after compound microorganism fermentation, coco coir and a binder, and the compound microorganism bacterium brick comprises 30 wt%-35 wt% of bacillus subtilis, 30 wt%-35 wt% of geotrichum candidum and 30 wt%-35 wt% of aspergillus niger. After fermentation with the compound microbial agent, the content of nutrient elements such as nitrogen, phosphorus and potassium in the vinasse is obviously increased, and the content of crude fibers is reduced. The invention further discloses a production process for preparing the microbial bacterial brick from the vinasse fermented by the compound microbial bacterial agent and application of the microbial bacterial brick to plant cultivation. The prepared microbial bacterial brick can effectively promote the growth of ryegrass. The method not only solves the problem of disposal of waste materials after wine brewing and recycles the waste materials, but also can be widely applied to the fields of agriculture, horticulture, ecological restoration and the like, and has a wide market prospect.

This invention relates to the field of bio-food fermentation technology, specifically a method for preparing solid-state fermented natto with lipid-lowering and thrombolytic functions. The method involves preparing a composite fermentation substrate using a mixture of soybeans, chickpeas, black beans, and red yeast rice. A specific combination of Bacillus subtilis, Monascus purpureus, and Aspergillus oryzae is then quantitatively introduced into this substrate for co-fermentation. Leveraging the symbiotic interaction of multiple microorganisms and their metabolic potential for deep degradation of macromolecules, this preparation pathway successfully promotes the simultaneous high-level expression of multiple targeted functional components. The resulting mass-produced solid-state fermented natto complex stably accumulates highly active nattokinase, monacolin K, and angiotensin-converting enzyme inhibitory small molecule proteins. The superposition of multiple core active substances enables the complex to exhibit synergistic effects in lowering blood lipids, efficiently dissolving thrombi, and assisting in lowering blood pressure.