41 results about "Aspergillus coreanus" patented technology

The invention relates to a microbiological treatment method for vegetables straw waste, belonging to the technical field of resources and environment. Based on the study on the composition and character of the vegetables straw waste, the invention provides a harmless treatment method. In the method, the vegetables straw waste is firstly concentrated and piled and then seeded for compound microbial fermentation, wherein the compound microorganism comprises bacillus subtilis, trichoderma and aspergillus niger. Under the compound microbial fermentation, the vegetables straw waste ferments and warms up constantly, so the harmful causative agents are exterminated, the vegetables straw waste is fully decomposed and good-quality organic fertilizer is obtained. The method has simple technology, can recycle vegetables straw waste, and is an ideal treatment method for the vegetables straw waste of a vegetable greenhouse with low cost and high efficiency.

A method for extracting the soluble calcium citrate by the manioc residue in a short period. The calcium citrate becomes more and more popular for the consumers as an ideal calcium supplement. The calcium citrate for sale in the market belongs to organic calcium, whose solubility is better than inorganic calcium and good for the absorption of human body to calcium, however, still belonging to the organic calcium with a low solubility, which restricts the absorption of human body to calcium. The inventive method mentioned above improves the absorption of the organic calcium and the mouthfeel by fermenting the manioc residue with Aspergillus niger. The produced organic calcium has a high solubility and tastes like lemon. The cost of the extracted soluble calcium citrate is equal to that of the citric acid with purity over 99.5%.

The invention relates to a novel method utilizing edible fungus residues to produce a dairy cattle protein feed through fermentation. The novel method comprises the following steps that 1, seed liquid is prepared: saccharomycetes 1, aspergillus 1 and lactic acid bacteria 1 are amplified and cultured respectively in culture mediums containing a carbon source, a nitrogen source and a restrictive nutrient source to obtain the seed liquid; 2, the edible fungus residues are processed: 30-40 parts of dried edible fungus residues, 45-55 parts of water, 1-5 parts of bran and 0.5-1 part of sugar (water adding is not needed for wet edible fungus residues) are mixed and stirred evenly; 3, the seed liquid obtained in the step 1 is inoculated in the edible fungus residues, the total inoculating quantity is 5-10%, and stationary culture is performed at the temperature of 35 DEG C for 5-7 days to obtain the dairy cattle protein feed. The protein content of the fungus residues is remarkably improved by utilizing microorganisms to ferment the edible fungus residues, coarse fiber can be converted into fiber which can be utilized by dairy cattle, waste produced in edible fungus production can be well utilized, corresponding economic value is created, and resources can be well recycled.

The invention relates to a strain capable of producing compound enzyme and a method of using the same to prepare compound enzyme for weaned piglets. The invention enables the problems that conventional feeds for weaned piglets are hard to digest and absorb and cause hypersensitivity to be effectively overcome. The strain provided in the invention is Aspergillus niger HKS11 which is preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 6027; and produced compound enzyme comprises protease, amylase and xylanase. The method of using the strain to prepare the compound enzyme comprises a step of activation of a culture, a step of acquisition of secondary seeds and a step of fermentation and culture in a solid fermentation cylinder. According to the invention, preparation cost is low, raw materials are rich and easily available, and energy saving and environmental protection are realized; when the produced compound enzyme for weaned piglets is added into a piglet feed, the utilization rate of the feed can be improved, productivity of the piglets is enhanced, and healthy growth of the piglets is promoted.

The invention relates to a preparation method of a long-chain alkane quaternization antibacterial agent, and a product and application of the antibacterial agent. Long-chain haloalkane is modified ona polymer richly containing tertiary amine; the carbon number on alkyl of the long-chain haloalkane is not smaller than 7. The prepared antibacterial agent has an excellent antibacterial effect on staphylococcus aureus, escherichia coli, candida albicans, helicobacter pylori, pseudomonas aeruginosa, aspergillus flavus and listeria monocytogenes; in the concentration dose with the antibacterial effect, cytotoxicity cannot be generated; the biological safety is realized. The preparation method is safe and simple and can be applied to industrial production; the development and application have development prospects.

The invention relates to a method for quantitatively and rapidly screening active materials for inhibiting the production of aflatoxin, which comprises the following steps: adding a microbial fermentation product, a plant extract, a natural product, or a chemical synthetic product with certain mass or concentration into a liquid culture medium, taking 200-500 microliters of obtained liquid, and adding the taken liquid into a micro-liquid incubator; by taking a blank nutrient solution as a contrast, inoculating spore suspension of 5-10 microliters of aspergillus parasiticus DM strains, culturing for 3-6 days at the temperature of 28 DEG C, visually inspecting the mycelial growth of the DM strains and the production of orange norsolorinic acid, and centrifuging the micro-liquid incubator with a visually inspected inhibition effect for 1 minute at a rotating speed of 1000 rpm; weighing the fresh weight of a mycelium, putting the mycelium into a methanol / sodium hydroxide solution, extracting norsolorinic acid therein for 30-40 minutes, measuring substances at 560 nm, and comparing the measured values with a light absorption value of an extracting solution; calculating a detoxification rate and a bacteriostasis rate; and selecting the substances with an inhibition rate of 90-100% as active materials for inhibiting the production of aflatoxin. The method disclosed by the invention can realize the trace, intuitive, quick, safe, and low-cost screening of active materials for efficiently inhibiting the production of aflatoxin.

The invention discloses a method for producing PUFA (polyunsaturated fatty acid) by utilizing potato in an Aspergillus niger xj strain. The method comprises the following steps of inoculating the Aspergillus niger xj strain in a PDA (potato dextrose agar) solid agarslantculture-medium to culture for three days at 27 DEG C and activate for three times, inoculating in a PDA liquid seed culture medium again to culture in a shake flask for three days at 27 DEG C and150rpm to obtain a seed liquid, transplanting the seed liquid in a 13L PDA fermentation medium according to a proportion of 5%(v / v) at 28+ / -1 DEG C and a stirring speed of 200-300rpm, controlling the ventilation amount within 1-2L / min, culturing for five days and stopping fermenting; and filtering through four layers of sterile gauze to remove mycelium, and collecting fermentation liquid to obtain the PUFA. According to the method for producing the PUFA, disclosed by the invention, the content of the obtained unsaturated fatty acid is high, the culture medium is easy to obtain, the fermentation conditions are stable, and the process is simple.

The application discloses a water-treated potassium ferrate sterilizing agent and a preparation method thereof. The water-treated potassium ferrate sterilizing agent is prepared by weighing and uniformly mixing potassium permanganate, concentrated hydrochloric acid, potassium hydroxide, iron nitrate nonahydrate, manganese dioxide, potassium chlorate, a polymerization inhibitor, triethylene tetramine, formaldehyde, phosphorus trichloride, a dispersing agent, sodium hydroxide, phosphorous acid, glycine and polyaspartic acid according to parts by weight. The melting point of the product is 205 DEG C; the scale inhibition rate is 96.9-98.9%; the sterilizing rate to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium, aspergillus, saprophytic bacteria and salmonella is 99-100%; when the water-treated potassium ferrate sterilizing agent is placed at 50-60 DEG C for a month, the sterilizing rate is 97.5-99.5%; when the water-treated potassium ferrate sterilizing agent is placed at 40-50 DEG C for half a year, the sterilizing rate is 96-99%; when the water-treated potassium ferrate sterilizing agent is placed at the room temperature for 1-2 years, the sterilizing rate is 95-97%.

The invention discloses a non-oxidized type compound bactericide for water treatment and a preparation method thereof. The non-oxidized type compound bactericide is prepared by the steps of weighing glutaraldehyde, acrylic acid-2-acrylamide-2-methyl propane sulfonic acid copolymer, tetradecyl benzyl dimethyl ammonium chloride, etidronic acid, water, PBTCA, a polymerization inhibitor, sulfonic acid, polyol phosphonate ester, PESA, a dispersant, sodium tungstate, bitertanol, sodium benzoate and acrylic acid-hydroxy-propyl acrylate copolymer in parts by weight, and mixing uniformly. The product is 100% water-soluble, and has a sterilizing rate of 99.9-100% to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella; and the sterilizing rate is 98-100% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 97.9-99.9% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 97.5-99.5% after the bactericide is placed for 1-2 years at room temperature.

The application discloses an imidazoline biguanide sulfate bactericide for water treatment and a preparation method of the bactericide. The preparation method comprises the following steps: according to parts by weight, weighing the following materials: thiourea dioxide, dodecyl trimethyl ammonium bromide, hydrogen peroxide, brominated 1-butyl-2,3-dimethylimidazole, water, dodecylguanidine acetate, a polymerization inhibitor, sodium metasilicate pentahydrate, sulfuric acid, hexadecyl dimethyl benzyl ammonium chloride, a dispersing agent, dioctadecyl dimethyl ammonium chloride, imidazoline, triethylene tetramine and ethanol, and uniformly mixing the weighed materials; the scale inhibiting ratio of the product is 70-90%, and sterilizing rate on escherichia coli, staphylococcus aureus, candida albicans, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella is 97.9-99.9%; when the bactericide is kept at 50-60 DEG C for one month, the sterilizing rate is 97.5-99.5%, when the bactericide is kept at 40-50 DEG C for half an year, the sterilizing rate is 97-99%, and when the bactericide is kept at room temperature for 1-2 years, the sterilizing rate is 94-98%.

The invention discloses a lauryl guanidine acetate bactericide for water treatment and a preparation method thereof. The lauryl guanidine acetate bactericide is prepared by the steps of weighing O-methyl-isourea sulfate, sodium hydroxide, sodium acetate, methanol, lauryl amine, ethanol, a polymerization inhibitor, acetic acid, anhydrous ethanol, dimethyl sulfate, a dispersant, urea, glacial acetic acid, amino trimethylene phosphonic acid and sodium hexametahposphate in parts by weight, and mixing uniformly. The purity of the bactericide is 96-100%; the sterilizing rate to escherichia coli, staphylococcus aureus, candida albicans, neisseria gonorrhoeae, penicillium notatum, aspergillus, saprophytic bacteria and salmonella is 98-100%; and the sterilizing rate is 97-99% after the bactericide is placed for one month at 50-60 DEG C, the sterilizing rate is 96-98% after the bactericide is placed for half a year at 40-50 DEG C, and the sterilizing rate is 94-98% after the bactericide is placed for 1-2 years at room temperature.

The invention provides an agricultural microbial enzyme, a bio-organic fertilizer and a preparation method, and belongs to the technical field of agricultural fertilizers. The agricultural microbial enzyme comprises the following raw materials in parts by weight: 10-50 parts of corn flour, 10-50 parts of wheat bran, 10-50 parts of rice husk, 0.01-1 part of a microbial agent and 20-50 parts of water. The microbial agent comprises bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus megatherium, bacillus mucilaginosus, bacillus laterosporus, aspergillus niger, saccharomycetes, lactic acid bacteria and actinomycetes. The agricultural microbial enzyme and the bio-organic fertilizer have higher stability and more active substances, can improve the utilization rate of the fertilizer, increase both production and income and improve soil, and have better pest and disease resistance effects.

The invention provides a biochar slow-release base fertilizer special for sweet potatoes. The biochar slow-release base fertilizer comprises a core layer and a coating layer coating the surface of the core layer. The core layer is formed by mixing of the following components by weight part: 60-80 of crop waste, 0.5-1.0 of Aspergillus oryzae, 6-8 of furfural residue, 2-4 of chili seed, 10-15 of clay, 15-20 of urea, 18-22 of calcium magnesium phosphate fertilizer, 30-40 of diammonium phosphate, 20-25 of potassium chloride, 2-4 of ferrous sulfate, 1-2 of chelated iron, 80-90 of biochar powder, and 0.2-0.4 of a fertilizer additive. The weight of the core layer accounts for 50%-60% of the total weight of the slow-release fertilizer. The coating layer is a mixture of biochar powder and a binder, and the weight of the coating layer accounts for 40%-50% of the total weight of the slow-release fertilizer. The nutrients of the fertilizer provided by the invention are slowly released under control, one-time fertilization can meet the demand of crops for fertilizer in the whole growth period, fertilizer consumption is reduced, and sweet potato yield is increased.

The invention relates to a bacterial strain with an antiviral activity and an application thereof. The bacterial strain is aspergillus tubingensis FJBJ11, and has already been registered and preserved in the common microorganism center of the china microorganism strain preservation management council on January 26th, 2015, and the preservation serial number is CGMCC No.10435. The bacterial strain is used for preparing a virus disease prevention preparation. According to the bacterial strain, an antiviral activity substance is separated and purified from bacterial strain fermentation liquid and developed into a microorganism source antiviral agent, and therefore the bacterial stain has wide application prospects. The activity substance prepared through the bacterial strain is high in antiviral activity, free from pollution to the environment, capable of being used for preventing and treating plant viruses caused by tobacco mosaic viruses (TMV), and suitable for application and popularization.

The invention provides a method for increasing the content of isoflavone aglycon in soybean paste, relating to a preparation process of the soybean paste and solving the problem that the content of isoflavone aglycon in the traditional soybean paste is lower. The method comprises the following steps of: (1) inoculating Aspergillus oryzae strains to carry out activation and amplified cultivation, so as to obtain a bacterial suspension with a spore density of 5*108cfu / ml; (2) mixing soybean and soybean germ in a certain proportion and adding water and soaking, draining and crushing into particles and cooking, cooling and inoculating, then moving into a yeast chamber and fermenting, and obtaining finished yeast when the activity of the beta-glycuronide in the yeast is more than 400U / g; and (3) crushing the finished yeast, adding water and salt in a certain proportion, mixing evenly and importing into a fermentation tank, and carrying out sealed fermentation at a constant temperature for 10-20 days. The total content of the isoflavone aglycon in the soybean paste made by the method of the invention is above 200microgram / g, which is much higher than the Daidzein content of 15-45microgram / g in the market soybean paste.

The invention discloses a high-performance antibacterial spraying-free POM composite material and a preparation method thereof, wherein the high-performance antibacterial spraying-free POM composite material specifically comprises the following raw materials by weight: 40-80 parts of a POM resin, 2-10 parts of metal toner, 1-8 parts of a dispersing agent and 5-15 parts of functionalized master batch, wherein the functionalized master batch comprises, by weight, 2-8 parts of an elastic ionomer, 2-6 parts of a nano-state aerogel and 2-6 parts of a silver ion antibacterial agent. According to theinvention, the key antibacterial agent component and the aerogel with a nanometer mesoporous structure are subjected to effective master batch premixing and adsorption, so that the good spraying-freeeffect is achieved, and the POM material has broad-spectrum antibacterial effect, wherein the long-acting antibacterial rates of the POM material to three different strains (escherichia coli, staphylococcus aureus and aspergillus niger) are respectively more than 99%; and by using the special toughening agent-ionomer, the spraying-free POM composite material has good rigidity and toughness balance.

The invention relates to a preparation method of a plastic long-acting mildew preventive, which comprises the following steps: pulverizing a mildew preventive active matter, and screening with a 80-200-mesh sieve; respectively crushing polycarbonate, a plasticizer, an antioxidant, a slow-release framework material, an inert auxiliary agent and a heat stabilizer, and sieving with an 80-200-mesh sieve; respectively mixing the mildew preventive active matter with the polycarbonate, the plasticizer, the antioxidant, the slow-release framework material, the inert auxiliary agent and the heat stabilizer according to a ratio, and dissolving the mixture into trichloromethane and / or ethanol; and mixing the mixture with a plastic polymer according to a ratio of 1: 20-100 at a temperature of 180-220DEG C, drying, and crushing to prepare the plastic long-acting mildew preventive. The mildewpreventive disclosed by the invention has a very good sterilization effect on common aspergillus niger, escherichia coli, mucor and candida, and the mildew preventive active matter is coated with the slow-release framework material, so that the sterilization lasting time is longer.

Belonging to the technical field of pharmaceutical chemistry, the invention discloses a naphthalimide benzimidazole compound, a preparation method and application thereof. The compound has strong in-vitro antimicrobial activity, especially shows very high inhibitory activity to staphylococcus aureus, methicillin-resistant staphylococcus aureus, bacillus subtilis, micrococcus luteus and other gram-positive bacteria, escherichia coli, proteus, pseudomonas aeruginosa, salmonella typhi and other gram-negative bacteria, candida utilis, aspergillus flavus, saccharomyces cerevisiae, candida albicans,candida mycoderma bacteria and other fungi, can be used for preparation of antibacterial and / or antifungal drugs, and is conducive to solving increasingly serious drug resistance, stubborn pathogenicmicroorganisms, newly emerging harmful microorganisms and other clinical treatment problems.

The invention discloses a water treatment ternary copolymerization bactericide and a preparation method thereof. The preparation method comprises the following steps: weighing glutaraldehyde, AMPS, crylic acid, isopropanol, water, sodium hydroxide, a polymerization inhibitor, sodium hypophosphite, maleic anhydride, ammonium persulfate, a dispersing agent, phosphorus trichloride, N,N-dimethylformamide, L-aspartic acid and glutamic acid according to parts by weight, and uniformly mixing the materials to prepare the bactericide. The scale inhibiting ratio of the bactericide is 85.5-98.5%; the density is 1.15-1.35 g / cm<2>; after the bactericide is stored at 50-60 DEG C for one month, the bactericidal rate is 98.5-99.5%; after the bactericide is stored at 40-50 DEG C for half a year, the bactericidal rate is 97-99%; after the bactericide is stored at the room temperature for 1-2 years, the bactericidal rate is 96-98%; when the bactericide is applied to escherichia coli, staphylococcus aureus, streptococcus albus, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella, the bactericidal rate is 98.8-99.8%.

The invention provides a method for detecting food-borne pathogenic bacteria in a microecological active bacterial preparation through multiplex PCR. For the method provided by the invention, the multiplex PCR technology is mainly adopted, and the method adopts the PCR reaction in which multiple specific primers are added in the same reaction system, and multiple nucleic acid fragments are simultaneously amplified. According to the method, the specific primers of seven common food-borne pathogenic bacteria including salmonella, escherichia coli, bacillus pumilus, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa and aspergillus niger are designed, seven target bands are simultaneously amplified in the same system, meanwhile, the specificity among each other is good, the limit of detection of the method achieves 200fg / [mu]l (template concentration), the corresponding concentration of bacterium is 120CFU / mL, the standard of the food safety can be achieved, the common pathogenic bacteria can cause the pathogenic effect when the concentration is 1000CFU / mL, therefore, the method is high in sensitivity, and is rapid, simple and convenient, and the cost is very low.

The invention discloses a method for treating and recycling liquid organic kitchen wastes. The method comprises the following steps: adjusting the initial pH value of the liquid kitchen waste to be 5.0-8.2; carrying out inoculating with an aspergillus fumigatus NJZ5 bacterial solution with a preservation number of CGMCC No.3309, a bacillus subtilis NJN-6 bacterial solution with a preservation number of CGMCC No.3183 and a bacillus amyloliquefaciens SQN-6 bacterial solution with a preservation number of CGMCC No.5808; and after the functional bacteria are inoculated, introducing sterilized airfor 4-8 minutes every 3-4 hours to enable the functional bacteria to be in an aerobic fermentation state, wherein the temperature of the liquid kitchen waste is controlled to be not lower than 30 DEGC in a fermentation treatment process, and the pH value is not less than 5.0. According to the method disclosed by the invention, the composite microorganisms are used for rapidly treating the liquidorganic kitchen waste, and substances in the liquid kitchen waste are completely decomposed. In a third day after treatment, the liquid kitchen waste is free of odor or peculiar smell, and the fermentation product is applied to farmland soil or is prepared into a commercial liquid composite microbial fertilizer.

The invention discloses a water treatment tertiary aminating resin bactericide and a preparation method thereof. The water treatment tertiary aminating resin bactericide can be prepared by matching and weighing benzene, micrococcus chlorinus, dimethylamine, hydrochloric acid, water, ethanol, a polymerization inhibitor, alkali, isopropanol, chlorotetradecane, a dispersing agent, chloroacetic acid, bisphenol-A, benzotriazole and PBTCA (phosphono butane-1,2,4-tricarboxylicacid) in parts by weight, uniformly mixing the raw materials and carrying out vacuum drying; and the water treatment tertiary aminating resin bactericide has a sterilizing rate of 98 to 100 percent on escherichia coli, staphylococcus aureus, canidia albicans, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella, has carrier content of 1 to 1.08mmol / g, has a corrosion rate of 0.0001 to 0.0021mm / a for tin brass, has a scale inhibition rate of 96 to 98 percent , has the sterilizing rate of 97.95 to 99.95 percent after being placed for one month at a temperature of 50 to 60 DEG C, has the sterilizing rate of 97.9 to 99.9 percent after being placed for half a year at a temperature of 40 to 50 DEG C, and has a sterilizing rate of 95 to 99 percent after being placed for 1 to 2 years at the room temperature.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses a gliotoxin 6-aromatic ring carboxylic ester compound (general formula I) which is designed and synthesized by taking a secondary metabolite gliotoxin of aspergillus fumigatus as a mother nucleus, and a preparation method thereof. The preparation method of the gliotoxin 6-aromatic ring carboxylate compound comprises the following steps: taking gliotoxin as an initial raw material, and carrying out esterification reaction on 6-site hydroxyl of the gliotoxin and aromatic ring or aromatic heterocyclic carboxylic acid on the premise of not damaging an active center disulfide bond of the gliotoxin, so as to obtain the gliotoxin 6-aromatic ring carboxylate compound. The inhibition activity of the series of compounds on histone lysine demethylase (LSD1) is obviously superior to that of maternal gliotoxin, and the series of compounds can be used for preparing anti-tumor drugs and can be applied to clinical treatment of diseases such as esophageal cancer, gastric cancer, lung cancer, colorectal cancer and breast cancer. The compound has a general formula I shown in the specification.

The invention belongs to the technical field of coatings, and particularly relates to a silver ion long-acting sterilization wall coating and a preparation method thereof. The invention relates to a silver ion long-acting sterilization wall coating, which is prepared from the following raw materials: electrolytic silver water, an active group-containing monomer, a surfactant and a filler. According to the prepared silver ion long-acting sterilization wall coating, under the condition that the specific electrolytic silver water concentration is required, the silver ions in the prepared wall coating do not easily fall off, the stability is high, and the long-acting sterilization effect is guaranteed. The silver ion long-acting sterilizing wall coating prepared by the invention can well inhibit wall mildewing, especially aspergillus niger, and also can ensure that the wall coating is used at a low temperature, so that the use environment and the application value of the coating are expanded.

The invention discloses an animal and plant waste decomposition complex microbial inoculant and a bio-organic fertilizer prepared from the same, and belongs to the field of bio-organic fertilizers. The microbial inoculant comprises bacillus subtilis, lactic acid bacteria, saccharomyces cerevisiae, trichoderma reesei and aspergillus niger, and by adding the microbial inoculant into animal and plantwaste decomposition fermentation, decomposition of the organic fertilizer can be accelerated, and the fermentation time can be shortened. The animal and plant waste decomposition complex microbial inoculant disclosed by the invention can be quickly propagated in decomposition production activities of the organic fertilizers, so that cellulose, protein and other substances in animal and plant wastes are degraded and decomposed, harmful substances are absorbed or decomposed, odor substances are absorbed or neutralized to achieve a deodorization effect, a certain biological prevention and control function is achieved, and soil physicochemical properties are improved.

The invention provides a complex microbial inoculant, a method for preparing a microbial composite carbon source by the complex microbial inoculant and application of the microbial composite carbon source in sewage treatment, and relates to the technical field of sewage treatment.The complex microbial inoculant comprises independently packaged composite microbial inoculants A and B. The composite microbial inoculant A comprises aspergillus fumigatus liquid and aspergillus oryzae liquid, the compound microorganism bacterium agent B comprises a bacillus licheniformis bacterium solution, a bacillus mucilaginosus bacterium solution, a bacillus subtilis bacterium solution and a candida krusei bacterium solution; the method for preparing the microbial composite carbon source from the composite microbial agent comprises the following steps: standing and precipitating, removing supernate, repeatedly treating, adding a calcium oxide solution, adjusting the pH value, reacting for 24-48 hours, adjusting the pH value again, mixing with the composite microbial agent B, fermenting, and adding the composite microbial agent A; when the COD (Chemical Oxygen Demand) concentration of the fermentation liquor is greater than or equal to 10000 mg / L, ending the fermentation; the purpose of sludge reduction is achieved, and the denitrification efficiency is further enhanced.

The invention relates to the field of freckle lightening cosmetics, in particular to a garlicin freckle lightening cosmetic and a preparation method thereof. The garlicin freckle lightening cosmetic comprises the preparation raw materials of A components, B components and C components, wherein the A components comprise the preparation raw materials in parts by weight of 2-5 parts of an emollient and 0.1-1 parts of a surfactant, the B components comprise the preparation raw materials of 80-90 parts of water, 7-10 parts of a skin-protecting agent, 0.05-0.15 part of a chelating agent, and 0.02-0.1 part of a pH regulation agent, and the C components comprise the preparation raw materials of 0.1-0.3 part of a preservative and 0.1-0.3 part of garlicin. The provided garlicin freckle lightening cosmetic is safe and free from stimulation, after the garlicin freckle lightening cosmetic is used for 5 weeks, the freckle lightening rate can achieve 84% or above, after the use of the garlicin freckle lightening cosmetic is stopped for 8 weeks, freckles do not recur, and besides, the garlicin freckle lightening cosmetic can also effectively resist bacteria, escherichia coli, candida albicans, aspergillus fumigatus, amebic protozoa and trichomonas vaginalis, so that people can prevent infection in daily life.

The invention discloses a primer composition for detecting pathogens of sepsis as well as a nucleic acid detection kit and a detection method of the primer composition. The invention discloses a primer composition for detecting pathogens of sepsis. The kit comprises a staphylococcus aureus primer group, a klebsiella pneumoniae primer group, an escherichia coli primer group, an enterococcus faecalis primer group, an acinetobacter baumannii primer group, a pseudomonas aeruginosa primer group, a stenotrophomonas maltophilia primer group, a streptococcus pneumoniae primer group, a neisseria meningitidis primer group and an aspergillus primer group. The primer group is at least one of an enterobacter cloacae primer group and a group B streptococcus primer group. According to the primer group for detecting the sepsis pathogens, the nucleic acid detection kit containing the primer group and the detection method provided by the invention, various sepsis pathogens can be rapidly and accurately detected, so that the defect that the existing sepsis pathogen detection technology is time-consuming and labor-consuming is overcome, the detection sensitivity and specificity are improved, the labor intensity is reduced, and the detection efficiency is improved. The detection period is shortened.