Method for detecting food-borne pathogenic bacteria in microecological active bacterial preparation through multiplex PCR

Abstract

The invention provides a method for detecting food-borne pathogenic bacteria in a microecological active bacterial preparation through multiplex PCR. For the method provided by the invention, the multiplex PCR technology is mainly adopted, and the method adopts the PCR reaction in which multiple specific primers are added in the same reaction system, and multiple nucleic acid fragments are simultaneously amplified. According to the method, the specific primers of seven common food-borne pathogenic bacteria including salmonella, escherichia coli, bacillus pumilus, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa and aspergillus niger are designed, seven target bands are simultaneously amplified in the same system, meanwhile, the specificity among each other is good, the limit of detection of the method achieves 200fg / [mu]l (template concentration), the corresponding concentration of bacterium is 120CFU / mL, the standard of the food safety can be achieved, the common pathogenic bacteria can cause the pathogenic effect when the concentration is 1000CFU / mL, therefore, the method is high in sensitivity, and is rapid, simple and convenient, and the cost is very low.

Description

[0001] 1 Technical field [0002] The invention relates to the field of food safety detection, in particular to a method for detecting whether food-borne pathogenic bacteria in a microecological live bacteria preparation exceeds the standard by multiple PCR technology. [0003] 2 Background technology [0004] Food-borne diseases refer to diseases caused by pathogenic factors such as toxic and harmful substances (including biological pathogens) that enter the human body through ingestion. Generally can be divided into infectious and toxic, including common food poisoning, intestinal infectious diseases, zoonotic diseases, parasitic diseases and diseases caused by chemical toxic and harmful substances. Food-borne pathogens can cause food-borne diseases or food poisoning, and even affect human safety, cause major economic and property losses, and reduce people's quality of life. [0005] According to the World Health Organization (WHO) report, there are up to 150 million cases of diarrh...

AI Technical Summary

Problems solved by technology

Due to the cumbersome procedures and time-consuming of these traditional detection methods

However, the micro-ecological living bacteria preparation itself contains a large number of microorganisms of different species, which makes the traditional detection method more complicated and the accuracy is also significantly reduced.

Images