Strain capable of producing compound enzyme and method of using same to prepare compound enzyme for weaned piglets

A technology of weaned piglets and compound enzymes, applied in the biological field, can solve problems such as intestinal damage, difficulty in digesting and absorbing feed, piglet diarrhea, etc., and achieve the effects of promoting healthy growth, promoting decomposition, digestion, absorption and utilization, and eliminating harmful effects

Active Publication Date: 2012-10-17
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] For above-mentioned situation, the object of the present invention is exactly to provide a kind of bacterial strain that produces compound enzyme and the method for preparing compound enzyme for weaned piglet, can effectively solve that weaned piglet is difficult to digest and absorb existing feed, causes the hypersensitivity reaction of weaned piglet, makes The intestinal tract is damaged, which eventually leads to problems with diarrhea in piglets

Method used

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  • Strain capable of producing compound enzyme and method of using same to prepare compound enzyme for weaned piglets
  • Strain capable of producing compound enzyme and method of using same to prepare compound enzyme for weaned piglets

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Embodiment 1

[0024] Aspergillus niger strain CGMCC No.6027 was fermented in a 100L solid fermenter to produce enzymes, and a ring of CGMCC No.6027 strains was added from the slant surface of the slant preservation medium to the slant surface of the primary seed medium to activate the strains. 32°C, after 72h to 96h of cultivation time, connect the three-ring first-level seed strains from the slant of the first-level seed medium into the second-level seed medium (secondary seed medium 60g / 1000mL triangular flask), at 28-32 °C, after 65h-84h of culture time, the inoculation amount of 0.2% of the weight of the fermentation medium was inserted into the fermentation medium in a 100L solid fermenter. The fermentation medium in the 100L solid fermenter is 50Kg, the temperature is 29°C, the relative humidity is above 90%, and the fermentation time is 24-32h. The aseptic air conditioning system and full atomization water replenishment system were opened in time to realize temperature self-control a...

Embodiment 2

[0027] Aspergillus niger strain CGMCC No.6027 was fermented in a 100L solid fermenter to produce enzymes, and a ring of CGMCC No.6027 strains was added from the slant surface of the slant preservation medium to the slant surface of the primary seed medium to activate the strains. 32°C, after 72h to 96h of cultivation time, connect the three-ring first-level seed strains from the slant of the first-level seed medium into the second-level seed medium (secondary seed medium 60g / 1000mL triangular flask), at 28-32 °C, after 65h-84h of culture time, the inoculation amount of 0.2% of the weight of the fermentation medium was inserted into the fermentation medium in a 100L solid fermenter. The fermentation medium in the 100L solid fermenter is 50Kg, the temperature is 29°C, the relative humidity is above 90%, and the fermentation time is 24-32h. The aseptic air conditioning system and full atomization water replenishment system were opened in time to realize temperature self-control a...

Embodiment 3

[0030] Aspergillus niger strain CGMCC No.6027 was fermented in a 100L solid fermenter to produce enzymes, and a ring of CGMCC No.6027 strains was added from the slant surface of the slant preservation medium to the slant surface of the primary seed medium to activate the strains. 32°C, after 72h to 96h of cultivation time, connect the three-ring first-level seed strains from the slant of the first-level seed medium into the second-level seed medium (secondary seed medium 60g / 1000mL triangular flask), at 28-32 °C, after 65h-84h of culture time, the inoculation amount of 0.2% of the weight of the fermentation medium was inserted into the fermentation medium in a 100L solid fermenter. The fermentation medium in the 100L solid fermenter is 50Kg, the temperature is 29°C, the relative humidity is above 90%, and the fermentation time is 24-32h. The aseptic air conditioning system and full atomization water replenishment system were opened in time to realize temperature self-control a...

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Abstract

The invention relates to a strain capable of producing compound enzyme and a method of using the same to prepare compound enzyme for weaned piglets. The invention enables the problems that conventional feeds for weaned piglets are hard to digest and absorb and cause hypersensitivity to be effectively overcome. The strain provided in the invention is Aspergillus niger HKS11 which is preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 6027; and produced compound enzyme comprises protease, amylase and xylanase. The method of using the strain to prepare the compound enzyme comprises a step of activation of a culture, a step of acquisition of secondary seeds and a step of fermentation and culture in a solid fermentation cylinder. According to the invention, preparation cost is low, raw materials are rich and easily available, and energy saving and environmental protection are realized; when the produced compound enzyme for weaned piglets is added into a piglet feed, the utilization rate of the feed can be improved, productivity of the piglets is enhanced, and healthy growth of the piglets is promoted.

Description

technical field [0001] The invention relates to the field of biology, in particular to a compound enzyme-producing bacterial strain and a method for preparing the compound enzyme for weaned piglets. Background technique [0002] With the development of the pig industry towards intensification and scale, early weaning of piglets has become an important method commonly used in pig production, and it is an advanced technology that is generally concerned in domestic and foreign pig production. It can improve the reproductive rate of sows , reduce the transmission of diseases from the mother to the piglets, and can improve the production performance and carcass quality during the growth period. However, the ensuing early weaning syndrome brings great losses to pig production. Piglets are affected by psychological, environmental and nutritional stress during early weaning, often manifested as poor appetite, digestive disorders, and diarrhea, which lead to growth The so-called "e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/62C12N9/42C12N9/30A23K1/165C12R1/685
Inventor 刘德海陈国参解复红贾彬权淑静马焕胡宜亮丁芳
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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