Production method of citric acid

A production method, citric acid technology, applied in the field of microbial fermentation, can solve the problems of accelerating the metabolic cycle of Aspergillus niger, reducing the metabolic rate of bacteria, and reducing the size of Aspergillus niger balls, so as to speed up the speed of entering and exiting cells, accelerate the metabolic cycle, and compare The effect of increased surface area

Active Publication Date: 2022-08-09
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that with acidity accumulation and bacterial colony aggregation in the citric acid fermentation process, the metabolism rate of the bacteria decreases and the fermentation period becomes longer, and a method for producing citric acid is provided, which is used to ferment and produce citric acid with biomass as raw material. During the mixing process, a certain proportion of titanium dioxide is added to make the Aspergillus niger balls smaller and increase the specific surface area during the stirring process. At the same time, it absorbs the toxins contained in the medium, improves the mass transfer performance of the medium, enhances cell viability, and accelerates the entry and exit of substances. Cell speed, so as to achieve the purpose of accelerating the metabolic cycle of Aspergillus niger

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  • Production method of citric acid

Examples

Experimental program
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Effect test

Embodiment 1

[0053] This example is used to illustrate the method for accelerating the metabolism of Aspergillus niger in the citric acid production process provided by the present invention

[0054] (1) Weigh 1kg of corn flour, wherein the particle size of corn flour is 1000-2000 μm.

[0055] (2) After mixing corn flour with 5 kg of water, sizing in a circulating water bath, the sizing temperature is 60°C, and the sizing time is 40 min.

[0056] (3) Use sodium hydroxide to adjust the pH of the product obtained by sizing to about 6, add amylase to carry out enzymatic hydrolysis and liquefaction, the addition amount of amylase is 20U / g dry base corn flour, the enzymatic hydrolysis temperature is 90 ℃, and the time is 1h .

[0057] (4) Mixing the liquefied product with a certain proportion of water to obtain a fermentation medium with a DE value of 18%. The seed liquid of Aspergillus niger was inoculated into the fermentation medium, the inoculation amount was 0.2 g, the fermentation pH wa...

Embodiment 2

[0062] This example is used to illustrate the method for accelerating the metabolism of Aspergillus niger in the citric acid production process provided by the present invention

[0063] (1) Take 1kg of dried potato, crush and sieve to obtain a particle size of 1000-2000 μm.

[0064] (2) Add 2 kg of water to the pulverized powder, and adjust the slurry in a circulating water bath for 1 hour. The temperature is 50°C, and the pH is adjusted to about 4.5.

[0065] (3) adding amylase to carry out enzymatic hydrolysis and liquefaction, the addition amount of amylase is 30U / g dry base potato, the temperature is 80°C, the pH is about 4.5, and the time is 40min.

[0066] (4) Mixing the liquefied product with a certain proportion of water to obtain a fermentation medium with a DE value of 18%. The seed liquid of Aspergillus niger was inoculated into the fermentation medium, the inoculum amount was 1 g, the fermentation pH was 4.5, and the temperature was 35° C. The fermentation was co...

Embodiment 3

[0071] This example is used to illustrate the method for accelerating the metabolism of Aspergillus niger in the citric acid production process provided by the present invention

[0072] (1) Take 1kg of corn stalks and pulverize, and the particle size of the material after pulverization is required to be 1200-2000 μm, and then subjected to steam explosion treatment.

[0073] (2) Add 10kg of water to the above-mentioned treated material, and adjust the slurry in a circulating water bath for 1 hour. The slurry temperature is 75°C and the pH is 5.

[0074] (3) Add amylase to carry out enzymatic hydrolysis and liquefaction. The addition amount of amylase is 50U / g dry basis corn stover, the temperature is 90℃, the pH is about 5, and the time is 4h.

[0075] (4) Mixing the liquefied product with a certain proportion of water to obtain a fermentation medium with a DE value of 18%. The seed liquid of Aspergillus niger was inoculated into the fermentation medium, the inoculation amoun...

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Abstract

The invention relates to the field of microbial fermentation and discloses a production method of citric acid. Including: (1) raw material pretreatment; (2) pulping; (3) liquefaction; (4) fermentation; (5) separation and purification of citric acid; wherein, in any one or several of steps (1)-(4) Titanium dioxide is added in this step. The method provided by the invention is to add a certain proportion of titanium dioxide in the process of fermenting and producing citric acid by using biomass as a raw material to make the Aspergillus niger balls smaller and increase the specific surface area during the stirring process, and at the same time adsorb the content contained in the culture medium. Toxins, improve the mass transfer performance of the medium, enhance cell viability, and speed up the rate of material entering and leaving cells, so as to achieve the purpose of accelerating the metabolic cycle of Aspergillus niger, thereby shortening the fermentation cycle, saving energy consumption, increasing production capacity, and reducing production costs.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for accelerating the metabolism of Aspergillus niger in the production process of citric acid by using titanium dioxide as a dispersing grinding accelerator. Background technique [0002] Citric acid, also known as citric acid, is a tricarboxylic acid compound, easily soluble in water, non-toxic, odorless, has a strong sour taste, is an important and multifunctional organic acid, Widely used in food, medicine, chemical industry and other fields. Citric acid is also the edible organic acid with the largest production and consumption in the world. With its wide application in emerging industries, its demand is growing at an annual rate of 5%. With the increasing demand year by year, improving the fermentation efficiency of citric acid has become a new research topic. This article analyzes the factors that limit the growth of citric acid from the perspective of the fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/48C12R1/685
CPCC12P7/48
Inventor 王迪领王法富秦慈进李长兴
Owner COFCO BIOTECHNOLOGY CO LTD
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