36 results about "Gliotoxin" patented technology

The invention relates to an organic materials zymolysis complex bacillus agent in the field of zymolysis bacillus agent technology. It comprises: low temperature bacteria, Bacillus cereus, gliotoxin, pseudomonas, slim streptomycete, high temperature bacteria and high temperature actinomyces with the weight ration 4:2:1:1:3:2:2. The low temperature bacterium is enzyme yeast with the heat resisting temperature below 20 deg. The high temperature bacterium is enzyme yeast with the heat resisting temperature below 70 deg. The heat resisting temperature below 70 deg of the high temperature actinomyces bellows 70 deg.

The invention relates to a dual regulating agent for preventing succession obstacle in protecting cultivation, with components according to following weight proportions: 1-10% of functional bacterial agent of bacillus, streptomycete or gliotoxin of 107-108 cfu / g counted by live bacterial content, 70-98% of progressional agent for organic functional expression such as fermentation organic fertilizer, grass peat or / and brown coal mixture, 5-20% of progressional agent for inorganic function such as zeolite, medical stone or shale. The invention discloses the agent and ite preparing method, and the action is realized by regulating both the immune and growth of root microecology and host, adsorbing functional bacterial onto prossessional agent, considering plant root system, root microorganism and root soil as a microecological system and regulating the population and quantity of microorganism in root system and physicochemical feature of soil to inhibit the population of pathogenic bacteria.

The invention provides composition containing gliotoxin. The composition comprises gliotoxin or a derivative thereof and compounds A, wherein the compounds A comprise multiple compounds. The composition has good control effect on multiple plant viruses, bacteria, powdery mildew and downy mildew and has a synergistic effect when compounded with selected components, the dosage is reduced, and the composition meets the requirements of environmental protection and future national policies.

The invention discloses an edyuillia FS110 glutathione S-transferase gene GliG promoter and application thereof. The nucleotide sequence of the promoter is as shown in SEQ ID NO.1. The upstream promoter sequence of the gene GliG is obtained by a chromosome walking technology, the core area of the promoter is predicted, and the edyuillia FS110 glutathione S-transferase gene GliG promoter is obtained. The promoter provided by the invention can efficiently start the expression of a hygromycin resistance gene hph, and the starting efficiency is obviously higher than that of a pgpdA promoter, so that a molecular biology base is laid for increasing the yield of gliotoxin through transcriptional control and heterogeneous expression in later period.

The invention belongs to the technical field of bioengineering and provides a high-yield bacterial strain for producing gliotoxin and a method for preparing the gliotoxin by adopting the bacterial strain for performing liquid fermentation. The bacterial strain for producing the gliotoxin related to the invention is a filamentous fungus, is identified as a Trichodermaviride through 16SrDNA, has a strain code of JBSH-002 and has a preservation number of CGMCC No.5611 at the Common Micro-organism Center of China Microbial Culture Preservation Commission. The bacterial strain can be applied to the production of the gliotoxin. The yield of the gliotoxin prepared by fermenting according to the method is above 500mg / L.

The invention discloses an application of two gliotoxins in the preparation of a biopesticide. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious inhibition effect on plant pathogenic fungi colletotrichum gloesporioides and alternaria alternate. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious plant pathogenic fungi inhibition effect and have good development and application prospects. Therefore, a candidate bio-pesticide is provided for research and development of novel plant pathogenic fungi and a scientific basis is provided for the development and utilization of natural active materials with a marine fungus source.

The invention relates to the field of regeneration and recycling of wastes and provides a harmless treatment method for gliotoxin mushroom dregs. The modified treatment utilization method of the gliotoxin mushroom dregs comprises the following steps: treating residual gliotoxin mushroom dregs; by taking the treated mushroom dregs as a main raw material and other agricultural byproducts as auxiliary materials, inoculating a selective multiple microorganism solid to ferment; and then performing drying, crushing and sieving, packaging and the like. The treatment method has the characteristics of being simple in production process, easy to operate, short in treatment time and the like. The proteins of a modified product is greater than or equal to 25%, and can be re-used for fermenting culture of gliotoxin, so that the invention provides a novel path which solves the environmental pollution and waste material recycle in gliotoxin production.

The present invention relates to a pesticide missible oil preparation, and in particular relates to a gliotoxin missible oil preparation, especially an environmentally friendly gliotoxin missible oil preparation safe to humans and crops. The gliotoxin missible oil preparation comprises the following raw materials by weight: 1% -10% of gliotoxin, 5%- 20% of a solvent, 5% -20% of an emulsifying agent, and 70% -88% of a co-solvent. The gliotoxin missible oil preparation uses gliotoxin as an effective active ingredient, and has higher bactericidal effect. Methyl oleate is used for replacing a light aromatic organic solvent in traditional missible oil, toxic and side effects due to heavy use of toluene and xylene can be solved, and the gliotoxin missible oil preparation is free of harm to human health, and free of environmental pollution.

The invention relates to the use of diketodithiopiperazine antibiotics, in particular chaetocin and gliotoxin, for the preparation of pharmaceutical compositions for antitumor therapy.

The invention discloses a method of promoting the Trichodermin production level of Trichoderma harziarum and belongs to the field of microorganism technology. The method promotes the concentration of Trichodermin in fermentation broth by adding the inactivated Rhizoctonia solani mycelium into the culture medium of Trichoderma harziarum.

The invention relates to a bactericidal composition containing gliomycin and pyrazole indanyl formamide, the active ingredients of which are gliomycin and pyrazole indanyl formamide, and the active ingredients of gliomycin and pyrazole indanyl The weight ratio of amides is 1:8-8:1, and the preferred composition ratio is 1:4-4:1. The fungicidal composition of the present invention can be used to prevent and treat common disease types such as late blight, downy mildew, gray mold, and powdery mildew on field crops, fruit trees, and vegetables. The synergistic effect of the mixed agent is improved, and it has special effects on wheat powdery mildew, ginger blast, watermelon wilt, etc.; the fungicide composition delays the rise of resistance caused by a single agent, and is of great significance to the comprehensive management of diseases and diseases.

The invention discloses a deep-sea fungus FS140 anti-gliotoxin self-protection gene GliM and an application of the deep-sea fungus FS140 anti-gliotoxin self-protection gene GliM. The nucleotide sequence of the anti-gliotoxin self-protection gene GliM is as shown in SEQ ID NO.1. Therefore, an anti-gliotoxin self-protection gene GliM sequence is obtained from a cDNA library of deep sea fungi FS140 and is successfully introduced into saccharomyces cerevisiae S.cerevisiae BJ5464 for anti-toxic function verification, so that the anti-gliotoxin capability of the saccharomyces cerevisiae is improved in the later period, and the anti-gliotoxin capability of the saccharomyces cerevisiae is improved. The heterologous expression level of the gliotoxin is improved, and a molecular biological foundation is laid for obtaining the novel gliotoxin.

The invention discloses a CRISPR / Cpf1 vector applicable to deep sea fungi FS140 as well as a construction method and application of the CRISPR / Cpf1 vector. According to the invention, the CRISPR / Cpf1 technology is utilized for the first time to construct a gene knockout system suitable for the deep-sea fungus G.pallida FS140, so that genetic engineering transformation of the G.pallida FS140 is promoted, and a molecular biology foundation is laid for clarification of a biosynthesis mechanism of G.pallida FS140 gliotoxin and acquisition of more gliotoxin derivatives with remarkable biological activity.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses a gliotoxin 6-aromatic ring carboxylic ester compound (general formula I) which is designed and synthesized by taking a secondary metabolite gliotoxin of aspergillus fumigatus as a mother nucleus, and a preparation method thereof. The preparation method of the gliotoxin 6-aromatic ring carboxylate compound comprises the following steps: taking gliotoxin as an initial raw material, and carrying out esterification reaction on 6-site hydroxyl of the gliotoxin and aromatic ring or aromatic heterocyclic carboxylic acid on the premise of not damaging an active center disulfide bond of the gliotoxin, so as to obtain the gliotoxin 6-aromatic ring carboxylate compound. The inhibition activity of the series of compounds on histone lysine demethylase (LSD1) is obviously superior to that of maternal gliotoxin, and the series of compounds can be used for preparing anti-tumor drugs and can be applied to clinical treatment of diseases such as esophageal cancer, gastric cancer, lung cancer, colorectal cancer and breast cancer. The compound has a general formula I shown in the specification.

The invention relates to the field of biotechnology, in particular to a group of high-affinity nucleic acid aptamers specifically binding to gliotoxin. According to the invention, high-affinity nucleic acid aptamers that specifically recognize gliotoxin are screened and obtained by adopting an oxidized graphene SELEX technique with gliotoxin as a target, and the performance of the nucleic acid aptamers is further improved through optimizing strategies such as truncation, mutation and the like. The group of nucleic acid aptamers has broad application prospects, and can be used for separation and removal of gliotoxin in complex systems, rapid detection and early diagnosis of gliotoxin in vitro and in vivo, neutralization of gliotoxin in related diseases or the development and preparation ofantagonistic drugs and other aspects.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses an L-amino acid-6-gliotoxin ester trifluoroacetate compound (general formula I) which is designed and synthesized by taking a secondary metabolite gliotoxin of aspergillus fumigatus as a mother nucleus and a preparation method thereof. The preparation method comprises the following steps: (1) taking gliotoxin as an initial raw material, carrying out esterification reaction on 6-site hydroxyl of the gliotoxin and N-Boc-amino acid on the premise that an active center disulfide bond of the gliotoxin is not damaged to obtain a gliotoxin 6-site amino-acid ester derivative, then removing a Boc protecting group in excessive trifluoroacetic acid, and salifying to obtain the L-amino acid-6-gliotoxin ester trifluoroacetate compound. The inhibitory activity of the compound on histone lysine demethylase (LSD1) is obviously superior to that of maternal gliotoxin, and the compound can be used for preparing anti-tumor drugs and is applied to clinical treatment of human esophageal cancer, gastric cancer, lung cancer, colorectal cancer, breast cancer and other diseases. The general formula I is shown in the specification.

The invention relates to the field of biotechnology, and specifically provides a group of high-affinity nucleic acid aptamers specifically binding to gliotoxin. The present invention takes gliotoxin as the target, uses graphene oxide SELEX technology to screen and obtain a high-affinity nucleic acid aptamer that specifically recognizes gliotoxin, and further improves the performance of the nucleic acid aptamer through optimization strategies such as truncation and mutation . The described group of nucleic acid aptamers has broad application prospects and can be used for the separation and removal of gliotoxins in complex systems, the rapid detection and early diagnosis of gliotoxins in vivo and in vitro, and the neutralization of gliotoxins in related diseases. Or the development and preparation of antagonistic drugs.

The invention discloses a seed medium and a fermentation medium for fermenting Aspergillus fumigatus to produce fumagillin, and also discloses a method for fermenting Aspergillus fumigatus to producefumagillin. The method comprises the following steps: 1, inoculating the seed medium with the Aspergillus fumigatus, and performing strain activation, wherein the pH value of the medium is natural; and 2, inoculating the fermentation medium with a micromonospora suspension prepared from the activated strain, performing fermentation culture, and collecting the fumagillin in the obtained fermentation solution, wherein the pH value of the fermentation medium is natural. A result of screening of carbon sources which affect the fumagillin shows that glycerol can be unexpectedly avoid the generationof gliotoxin and other byproducts as a carbon source, so subsequent separation and purification are avoided, the production cost is reduced, and factory large-scale production is easy.

The invention especially relates to a bactericidal composition containing gliotoxin and fenhexamid, belonging to the technical field of pesticide mixtures. The bactericidal composition contains the following active components: gliotoxin and fenhexamid in a weight ratio of 1: 10 to 10: 1. The bactericidal composition provided by the invention broadens the application scope of individual gliotoxin and individual fenhexamid, and through compounding of gliotoxin and fenhexamid, pathogenic fungi and bacteria can be simultaneously prevented and treated, so a plurality of diseases can be prevented and treated through application of the bactericidal composition once. Gliotoxin and fenhexamid are subjected to two-component compounding, the advantages of gliotoxin and fenhexamid complement each other, and the synergistic pesticide effect of secondary metabolites is improved; moreover, plants may better resist infection by external pathogens through stimulation of cell division and increase in the thickness of cell walls; thus, the problems of poor prevention effect, high resistance and the like caused by usage of a single pesticide are effectively overcome, the service life of the bactericidal composition is prolonged, and the bactericidal composition is of critical significance to comprehensive prevention and treatment of common diseases in agriculture.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses a L-amino acid-6-gliotoxin ester trifluoroacetate compound ( General formula I) and its preparation method. Its preparation method: (1) using gliotoxin as the starting material, under the premise of not destroying the disulfide bond of its active center, its 6-position hydroxyl group undergoes esterification reaction with N-Boc-amino acid to obtain the 6-position of gliotoxin Amino acid ester derivatives, then remove the Boc protecting group in excess trifluoroacetic acid, and form a salt to obtain L-amino acid-6-glitoxin ester trifluoroacetic acid salt compounds. The inhibitory activity of this type of compound on histone lysine demethylase (LSD1) is significantly better than that of its parent gliotoxin, which can be used to prepare antineoplastic drugs and be used in the clinical treatment of human esophageal cancer, gastric cancer, lung cancer, and colorectal cancer. cancer and breast cancer. Its general formula is as follows: general formula Ⅰ.

The invention discloses a CRISPR / Cpf1 vector suitable for deep-sea fungus FS140, its construction method and application. This invention uses CRISPR / Cpf1 technology for the first time to construct a gene knockout system suitable for the deep-sea fungus G.pallida FS140, thereby promoting the genetic engineering transformation of G.pallida FS140 and elucidating the biosynthesis mechanism of G.pallida FS140 gliotoxin And obtain more gliotoxin derivatives with significant biological activity to lay the foundation of molecular biology.

The invention relates to the fields of medicinal chemistry and microbial pharmacy, and discloses a gliotoxin 6-aromatic ring carboxylate compound (general formula I) designed and synthesized with the secondary metabolite of Aspergillus fumigatus gliotoxin as the parent nucleus. and its preparation method. Its preparation method: using gliotoxin as a starting material, under the premise of not destroying the disulfide bond of its active center, the 6-hydroxyl group undergoes an esterification reaction with an aromatic ring or an aromatic heterocyclic carboxylic acid to obtain the gliotoxin 6- Aromatic ring carboxylates. The inhibitory activity of this series of compounds on histone lysine demethylase (LSD1) is significantly better than that of its parent gliotoxin, and can be used for the preparation of antitumor drugs for clinical treatment of human esophageal cancer, gastric cancer, lung cancer, and colorectal cancer. cancer and breast cancer. It has the following general formula: general formula I.

The invention discloses a deep sea fungus FS140 oxidordeuctase gene GliT promoter and an application thereof. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1. An upstream promoter sequence of a GliT gene is obtained through a chromosome walking technology, a promoter core region is predicted, a core region GliT0 of a deep sea fungus FS140 oxidordeuctase gene GliT promoter is obtained, and a key core region of the GliT promoter in a prokaryotic and eukaryotic expression system is successfully verified through point mutation. The promoter disclosed by the invention not only can efficiently promote the expression of hygromycin resistance gene hph, but also has the promotion efficiency similar to that of a constitutive promoter TEF1; the expression of the ampicillin Amp can be effectively promoted, so that a molecular biological foundation is laid for increasing the yield of the gliotoxin and obtaining more novel gliotoxin with high activity through transcription regulation and heterologous expression in the later period.

Disclosed is a composition and method for a therapeutic treatment that is able to combat certain conditions such as alcohol dependence, opioid abuse treatment, neurological disorders, neuropathic pain, and fibromyalgia. The novel gliotoxin analog compound acts by acting as an antagonist to one or more opioid receptors, which, when present leads to the inhibition of conditions, providing increased performance over known treatments. The disclosed compounds also shows the ability to cross the blood-brain-barrier in a highly efficient manner.

The invention discloses an edyuillia FS110 glutathione S-transferase gene GliG promoter and application thereof. The nucleotide sequence of the promoter is as shown in SEQ ID NO.1. The upstream promoter sequence of the gene GliG is obtained by a chromosome walking technology, the core area of the promoter is predicted, and the edyuillia FS110 glutathione S-transferase gene GliG promoter is obtained. The promoter provided by the invention can efficiently start the expression of a hygromycin resistance gene hph, and the starting efficiency is obviously higher than that of a pgpdA promoter, so that a molecular biology base is laid for increasing the yield of gliotoxin through transcriptional control and heterogeneous expression in later period.

This invention relates to methods and compositions for detecting, quantifying, or identifying mycotoxins. More particularly, the invention relates to methods and compositions for detecting, quantifying, or identifying a gliotoxin, or a derivative thereof, a mycotoxin of a Penicillium species, or a mycotoxin of a Chaetomium species, in the tissues or body fluid samples of patients.

The invention discloses oxidoreductase GliT and application thereof in resisting mycotoxins. The amino acid sequence of the oxidoreductase GliT is shown as SEQ ID NO. 2. In view of the fact that no research on resistance of deep sea fungus G.pallida FS140 to various mycotoxins exists at present, the gene sequence of the oxidoreductase GliT is obtained from a cDNA library of the deep sea fungus FS140 and is successfully introduced into saccharomyces cerevisiae S.cerevisiae BJ5464 for antitoxic function verification, so that a molecular biology foundation is laid for improving the later capacity of the saccharomyces cerevisiae to resist gliotoxin and derivatives thereof, trichothecene toxins, Eupeiflin and dicererol C toxins, improving the heterologous expression level of related mycotoxins and analyzing the action mechanism of the related mycotoxins. In addition, the novel oxidoreductase GliT is further separated and purified, whether the oxidoreductase GliT has a degradation effect on the related mycotoxins is determined, and a new idea is provided for developing economic and feasible toxicity reducing reagents, mycotoxins prevention and control additives and the like.

Disclosed is a composition and method for a therapeutic treatment that is able to combat certain conditions such as alcohol dependence, opioid abuse treatment, neurological disorders, neuropathic pain, and fibromyalgia. The novel gliotoxin analog compound acts by acting as an antagonist to one or more opioid receptors, which, when present leads to the inhibition of conditions, providing increased performance over known treatments. The disclosed compounds also shows the ability to cross the blood-brain-barrier in a highly efficient manner.

The invention discloses an application of an anti-gliotoxin self-protection gene GliK in assisting host cells in resisting gliotoxin. The nucleotide sequence of the anti-gliotoxin self-protection gene GliK is as shown in SEQ ID NO.1. Therefore, an anti-gliotoxin self-protection gene GliK sequence is obtained from a cDNA library of deep sea fungi FS140 and is successfully introduced into saccharomyces cerevisiae S.cerevisiae BJ5464 for anti-toxic function verification, so that the anti-gliotoxin capability of the saccharomyces cerevisiae is improved in the later period, the heterologous expression level of the gliotoxin is improved, and a molecular biological foundation is laid for obtaining the novel gliotoxin.