Medium and culture method for fermenting Aspergillus fumigatus to produce fumagillin

A fermentation medium, fumagillin technology, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve problems such as increasing process difficulty

Active Publication Date: 2018-06-15
HENAN INST OF SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Medium and culture method for fermenting Aspergillus fumigatus to produce fumagillin
  • Medium and culture method for fermenting Aspergillus fumigatus to produce fumagillin
  • Medium and culture method for fermenting Aspergillus fumigatus to produce fumagillin

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A seed culture medium for producing fumagillin by Aspergillus fumigatus, each liter of the seed culture medium includes the following components in mass volume ratio: glycerol 22.0~50.0g / L, MgSO 4 ·7H 2 O 0.1~0.5g / L, NaCl 10.0~20.0g / L, KH 2 PO 4 0.1~0.5g / L, FeSO 4 ·7H 2 O 0.013g / L, CuSO 4 ·5H 2 O 0.004g / L, CaCO 3 1~5g / L, peptone 10~30g / L, agar 15~20g / L, the balance is water.

[0026] A fermentation medium for producing fumagillin by fermentation of Aspergillus fumigatus, the seed medium per liter includes the following components in mass-to-volume ratio: 20-50 g / L of glycerol, 1-10 g / L of yeast extract powder, MgSO 4 ·7H 2 O 1~10 g / L, KCl 1~10 g / L, K 2 HPO 4 5~30g / L,FeSO 4 ·7H 2 O 0.013 g / L, corn flour 1~20 g / L, the balance is water. Among them: glycerol, as a carbon source; yeast extract powder, as an organic nitrogen source; corn flour, as a growth factor.

Embodiment 2

[0028] A method for producing fumagillin by Aspergillus fumigatus fermentation, comprising the following steps:

[0029] Step 1. Inoculate the Aspergillus fumigatus in the seed medium to activate the strain, and the pH value of the culture medium is natural; the activation conditions of the strain are: temperature 25-35°C, culture period 6-10 days;

[0030]Step 2: inoculate the monospore suspension prepared by activated strains on the fermentation medium, the pH of the fermentation medium is natural, carry out fermentation culture, and collect the fumagillin in the fermentation broth. Fermentation was carried out by means of shake flask fermentation, the fermentation culture temperature of the shake flask fermentation was 30°C, and the shaker rotation speed was 180 rpm; the glass beads were 6 mm in diameter, and the addition amount was 10; the inoculum volume percentage was 1%, The mass-volume ratio of corn flour is 1~20 g / L, and the liquid volume is 50mL / 250 mL; the whole fer...

Embodiment 3

[0032] A method for producing fumagillin by Aspergillus fumigatus fermentation, comprising the following steps:

[0033] Step 1. Inoculate the Aspergillus fumigatus in the seed medium to activate the strain, and the pH value of the medium is natural; the activation conditions of the strain are: temperature 30°C, culture period 8 days;

[0034] Step 2: inoculate the monospore suspension prepared by activated strains on the fermentation medium, the pH of the fermentation medium is natural, carry out fermentation culture, and collect the fumagillin in the fermentation broth. Fermentation was carried out in a 5 L fermenter, and the fermentation conditions of the 5 L tank were: temperature 30°C, rotation speed 800 rpm, oxygen flux 2 L / min, inoculum volume percentage 1%, corn flour mass volume ratio 1.7 g / L, pH 6.2.

[0035] Using glycerol as a carbon source, the content of fumagillin in 5 L fermenter fermentation culture is 90-150 mg / L, such as figure 1 As shown, through high-per...

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Abstract

The invention discloses a seed medium and a fermentation medium for fermenting Aspergillus fumigatus to produce fumagillin, and also discloses a method for fermenting Aspergillus fumigatus to producefumagillin. The method comprises the following steps: 1, inoculating the seed medium with the Aspergillus fumigatus, and performing strain activation, wherein the pH value of the medium is natural; and 2, inoculating the fermentation medium with a micromonospora suspension prepared from the activated strain, performing fermentation culture, and collecting the fumagillin in the obtained fermentation solution, wherein the pH value of the fermentation medium is natural. A result of screening of carbon sources which affect the fumagillin shows that glycerol can be unexpectedly avoid the generationof gliotoxin and other byproducts as a carbon source, so subsequent separation and purification are avoided, the production cost is reduced, and factory large-scale production is easy.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation engineering, in particular to a medium and a culture method for fermenting Aspergillus fumigatus to produce fumagillin. Background technique [0002] Fumagillin is an important secondary metabolite produced by the fermentation of Aspergillus fumigatus. Honey bee microsporidiosis is a global disease. It is a parasitic disease caused by honeybee sporozoites parasitizing the intestinal epithelial cells of honey bees. The resistance of bees infected by microsporidia is weakened, which often causes bees to be infected with various diseases such as, Malanobiosis, chronic paralysis, spirochete and chalk disease, etc., will bring immeasurable economic losses to beekeepers once they occur. Fumagillin is the only drug recognized and used in the prevention and treatment of bee microsporidiosis in the United States, Canada and other countries. Fumagillin is used to prevent and control bee micr...

Claims

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Application Information

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IPC IPC(8): C12P17/16C12R1/68
CPCC12P17/162
Inventor 王昊鹏吴黎明倪辉肖安风赵柳微杜开书张中印
Owner HENAN INST OF SCI & TECH
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