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Method for improving gliotoxin production level of harziarum strain

A technology of Trichoderma harzianum and trichoderma, which is applied in the field of microorganisms, can solve the problems of low production capacity and low level of production, and achieve the effect of improving the level of production and reliable.

Inactive Publication Date: 2008-09-24
CHINA JILIANG UNIV
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

Wild-type strains isolated from nature often have low levels of production and low production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The cultivation of Rhizoctonia solani: culture medium is potato dextrose liquid medium (PD), 300mL triangular bottle packs PD liquid medium 60mL, picks a little Rhizoctonia solani mycelium in the sterilized culture bottle with inoculation hook, Place on a shaker at 26°C to 28°C, 200r / min, and shake for 6 days. The fermentation broth is centrifuged at 5000r / min for 10min, and the mycelium is collected. Vacuum freeze-drying at -53°C and 0.5mbar for 15h, the inactivated bacteria Grind the filaments into small particles for later use;

[0021] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780 see the publication of patent application 200710066843.1, the publication number is CN...

Embodiment 2

[0025] The cultivation of Rhizoctonia solani: with embodiment 1;

[0026] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780 see the publication of patent application 200710066843.1, the publication number is CN101037656. Fermentation medium formula: its components and content (mass contained in each liter of medium) are 90 mg of small particles of Rhizoctonia solani mycelium, 30 g of peptone, 10 g of beef extract, 24 g of glucose, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no Rhizoctonia solani mycelium was added in the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium in a 300mL triangular bottle w...

Embodiment 3

[0030] The cultivation of Rhizoctonia solani: with embodiment 1;

[0031] Liquid fermentation of Trichoderma harzianum: Trichoderma harzianum is an endophytic fungus isolated from Chinese wolfberry. This strain has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was August 14, 2006, and the preservation registration number was CGMCC No. .1780. Fermentation medium formula: its component and content (mass contained in every liter of medium) are 90mg of small particles of Rhizoctonia solani mycelium (added after 24 hours of Trichoderma harzianum liquid fermentation), peptone 30g, beef Extract 10g, glucose 24g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no Rhizoctonia solani mycelium was added in the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium in a 300mL triangular bottle was used to draw 1mL of Trichoderma harzi...

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PUM

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Abstract

The invention discloses a method of promoting the Trichodermin production level of Trichoderma harziarum and belongs to the field of microorganism technology. The method promotes the concentration of Trichodermin in fermentation broth by adding the inactivated Rhizoctonia solani mycelium into the culture medium of Trichoderma harziarum.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for increasing the toxin production level of Trichoderma harzianum. Background technique [0002] The titer in microbial fermentation is often the bottleneck that determines the production cost and realizes industrial production. Genetic modification of strains, optimization of culture medium and culture conditions are common methods to improve the production level. In recent years, fungal elicitors have been widely used in the research of plant cell culture and microbial fermentation to produce secondary products. The research team isolated and screened an active endophytic fungus, Trichoderma harzianum, that produces Trichodermin from Lycium chinensis. Trichoderma has a strong inhibitory effect on a variety of crop pathogenic fungi, and has broad application prospects in the control of crop diseases. Wild-type strains isolated from nature often have low levels of h...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/885
Inventor 申屠旭萍俞晓平王为民董胜张
Owner CHINA JILIANG UNIV
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