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Method for improving trichodermin content in fermentation liquor of Trichoderma harzianum strain L

A technology of fermentation broth and trichoderma, which is applied in the field of microorganisms, can solve the problems of low production capacity and low level of hormone production, and achieve the reliable effect of improving the level of production of hormones.

Inactive Publication Date: 2011-02-23
CHINA JILIANG UNIV
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

Wild-type strains isolated from nature often have low levels of production and low production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Cucumber Fusarium wilt culture: purchased from Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences. The medium is potato dextrose liquid medium (PD), 60mL of PD liquid medium in a 300mL triangular bottle, pick a little mycelium of Fusarium wilt fungus with an inoculation hook, put it in a sterilized culture bottle, and place it on a shaker at 26°C to 28°C, 200r / min, shaking culture for 6 days, the fermentation broth was centrifuged at 5000r / min for 10min, the mycelium was collected, and vacuum freeze-dried at -53°C and 0.5mbar for 15h, and the inactivated mycelium was in a mortar under sterile conditions Grind to powder, set aside;

[0021] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from Lycium chinensis. This strain has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms. The preservation date was Augu...

Embodiment 2

[0025] The cultivation of cucumber wilt pathogen: with embodiment 1;

[0026] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from Chinese wolfberry, and the fermentation medium formula: its components and content (mass contained in each liter of medium) are Fusarium wilt of cucumber Silk powder 90mg, peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no inactivated mycelium powder of Fusarium wilt of cucumber was added to the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium was packed in a 300mL triangular bottle, and 1mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 1 / mL) in a sterilized culture bottle, placed on a shaker at 28°C, 180r / min, shaking culture for 96h, the fermentation broth was centrifuged at 5000r / min for 10min, and ...

Embodiment 3

[0030] The cultivation of cucumber wilt pathogen: with embodiment 1;

[0031] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from Chinese wolfberry, and the fermentation medium formula: its components and content (mass contained in each liter of medium) are Fusarium wilt of cucumber Silk powder 90mg (added after Trichoderma harzianum liquid fermentation for 24 hours), peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; no inactivated mycelium powder of Fusarium wilt of cucumber was added to the control, and the remaining components and contents were the same as the fermentation medium formula; 60mL of fermentation medium was packed in a 300mL triangular bottle, and 1mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 each / mL) in a sterilized culture bottle, placed on a shaker at 28°C, 180r / min, shaking culture for 10...

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Abstract

The invention discloses a method for improving trichodermin content in fermentation liquor of Trichoderma harzianum strain L, and belongs to the technical field of microorganisms. The method comprises the following steps of: adding inactivated Fusarium oxysporum mycelium powder into a culture medium of the Trichoderma harzianum strain L for performing fermentation culture; performing centrifugation on the fermentation liquor; analyzing the trichodermin content in the fermentation liquor by adopting gas chromatography; and displaying that the concentration of trichodermin in the fermentation liquor after adding the inactivated Fusarium oxysporum mycelium powder is increased by several times. The invention provides a feasible method for improving the fermentation level for producing the trichodermin of the Trichoderma harzianum strain L.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for increasing the level of trichoderma harzianum producing trichoderma. Background technique [0002] The titer in microbial fermentation is often the bottleneck that determines the production cost and realizes industrial production. Genetic modification of strains, optimization of culture medium and culture conditions are common methods to improve the production level. In recent years, fungal elicitors have been widely used in the research of plant cell culture and microbial fermentation to produce secondary products. Fungal elicitors are mainly fungal cell surface structures or secretions, which can be fungal mycelium, mycelial degradation products, fermentation broth, fungal secretions, etc., mainly including: polysaccharides, chitosan, β- 1-3-glucan, acetylglucuronic acid, glycoprotein, protein, short peptide, unsaturated fatty acid, etc. There are few rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01P3/00C12P7/62A01N63/04C12R1/885C12R1/77
Inventor 俞晓平申屠旭萍董胜张郝培应边亚琳
Owner CHINA JILIANG UNIV
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