Application of Fusarium oxysporiumf.sp.cucumeris in improving level of producing trichodermin of trichoderma harziarum strain L
A technology of cucumber Fusarium wilt and Trichoderma, which is applied in the field of microorganisms, can solve the problems of low production capacity, low level of hormone production, etc., and achieve the effect of improving the level of protein production and reliable.
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Embodiment 1
[0020] Cultivation of Fusarium wilt of cucumber: purchased from Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences. The medium is potato dextrose liquid medium (PD), and 60 mL of PD liquid medium in a 300 mL triangular bottle. Use an inoculation hook to pick a little cucumber Fusarium wilt mycelium and place it in a sterilized culture flask. Place it on a shaker at 26℃~28℃, 200r / min, shaking culture for 6 days, the fermentation broth was centrifuged at 5000r / min for 10min, the mycelium was collected, and vacuum freeze-dried at -53℃, 0.5mbar for 15h. The inactivated mycelium was used in a mortar under aseptic conditions Grind to powder, set aside;
[0021] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from corn bones. This strain has been deposited in the General Microbiology Center of the China Microbial Species Collection Management Committee. The preservation date is...
Embodiment 2
[0025] Cultivation of Fusarium wilt of cucumber: same as Example 1;
[0026] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from citron bone. The fermentation medium formula: its components and content (the mass contained in the medium per liter) are Cucumber Fusarium wilt Silk powder 90mg, peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; No inactivated cucumber Fusarium wilt mycelium powder was added to the control, and the rest of the components and contents were the same as the fermentation medium formula; 300 mL triangular flasks were packed with 60 mL of fermentation medium, and 1 mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 (Pieces / mL) in a sterilized culture flask, placed on a 28°C shaker, 180r / min, shaking culture for 96h, the fermentation broth was centrifuged at 5000r / min for 10min, and the supernata...
Embodiment 3
[0030] Cultivation of Fusarium wilt of cucumber: same as Example 1;
[0031] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from citron bone. The fermentation medium formula: its components and content (the mass contained in the medium per liter) are Cucumber Fusarium wilt Silk powder 90mg (added after 24 hours of liquid fermentation with Trichoderma harzianum), peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; No inactivated cucumber Fusarium wilt mycelium powder was added to the control, and the rest of the components and contents were the same as the fermentation medium formula; 300 mL triangular flasks were packed with 60 mL of fermentation medium, and 1 mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 (Pieces / mL) in a sterilized culture flask, placed on a 28°C shaker, 180r / min, shaking culture for 108h, the fe...
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