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Application of Fusarium oxysporiumf.sp.cucumeris in improving level of producing trichodermin of trichoderma harziarum strain L

A technology of cucumber Fusarium wilt and Trichoderma, which is applied in the field of microorganisms, can solve the problems of low production capacity, low level of hormone production, etc., and achieve the effect of improving the level of protein production and reliable.

Inactive Publication Date: 2012-07-04
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild-type strains isolated from nature often have low levels of production and low production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Cultivation of Fusarium wilt of cucumber: purchased from Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences. The medium is potato dextrose liquid medium (PD), and 60 mL of PD liquid medium in a 300 mL triangular bottle. Use an inoculation hook to pick a little cucumber Fusarium wilt mycelium and place it in a sterilized culture flask. Place it on a shaker at 26℃~28℃, 200r / min, shaking culture for 6 days, the fermentation broth was centrifuged at 5000r / min for 10min, the mycelium was collected, and vacuum freeze-dried at -53℃, 0.5mbar for 15h. The inactivated mycelium was used in a mortar under aseptic conditions Grind to powder, set aside;

[0021] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from corn bones. This strain has been deposited in the General Microbiology Center of the China Microbial Species Collection Management Committee. The preservation date is...

Embodiment 2

[0025] Cultivation of Fusarium wilt of cucumber: same as Example 1;

[0026] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from citron bone. The fermentation medium formula: its components and content (the mass contained in the medium per liter) are Cucumber Fusarium wilt Silk powder 90mg, peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; No inactivated cucumber Fusarium wilt mycelium powder was added to the control, and the rest of the components and contents were the same as the fermentation medium formula; 300 mL triangular flasks were packed with 60 mL of fermentation medium, and 1 mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 (Pieces / mL) in a sterilized culture flask, placed on a 28°C shaker, 180r / min, shaking culture for 96h, the fermentation broth was centrifuged at 5000r / min for 10min, and the supernata...

Embodiment 3

[0030] Cultivation of Fusarium wilt of cucumber: same as Example 1;

[0031] Liquid fermentation of Trichoderma harzianum strain L: Trichoderma harzianum strain L is an endophytic fungus isolated from citron bone. The fermentation medium formula: its components and content (the mass contained in the medium per liter) are Cucumber Fusarium wilt Silk powder 90mg (added after 24 hours of liquid fermentation with Trichoderma harzianum), peptone 30g, corn flour 10g, glucose 15g, bran 5g, MnCl 2 0.01g, KH 2 PO 4 0.1g, MgSO 4 0.05g; No inactivated cucumber Fusarium wilt mycelium powder was added to the control, and the rest of the components and contents were the same as the fermentation medium formula; 300 mL triangular flasks were packed with 60 mL of fermentation medium, and 1 mL of Trichoderma harzianum strain L spores were sucked with a sterile pipette Suspension (1×10 6 (Pieces / mL) in a sterilized culture flask, placed on a 28°C shaker, 180r / min, shaking culture for 108h, the fe...

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Abstract

The invention discloses application of Fusarium oxysporiumf.sp.cucumeris in improving level of producing trichodermin of a trichoderma harziarum strain L, and belongs to the technical field of microorganisms. The method comprises the following steps of: adding inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder into a culture medium for the trichoderma harziarum strain L for fermentation culture; centrifuging fermentation liquor; and analyzing the content of the trichodermin in the fermentation liquor by adopting a gas chromatogram, wherein the concentration of the trichodermin in the fermentation liquor after the inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder is added is improved by several times than that of a contrast. The invention provides a feasible method for improving the fermentation level of producing the trichodermin of the trichoderma harziarum strain L.

Description

Technical field [0001] The present invention relates to the field of microbial technology, in particular to a method for increasing the level of trichomycin produced by Trichoderma harzianum. Background technique [0002] In microbial fermentation, titer is often the bottleneck that determines production costs and realizes industrial production. Genetic modification of strains, optimization of medium and culture conditions, etc. are common methods to improve the level of production of nutrients. In recent years, fungal elicitors have been widely used in the research of plant cell culture and microbial fermentation to produce secondary products. Fungal elicitors are mainly fungal cell surface structures or secretions. They can be fungal mycelium, mycelial degradation products, fermentation broth, fungal secretions, etc., mainly including: polysaccharides, chitin, β- 1-3-glucan, acetyl glucuronic acid, glycoprotein, protein, short peptide, unsaturated fatty acid, etc. There are ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62A01N63/04A01P3/00C12R1/885C12R1/77
Inventor 俞晓平申屠旭萍郝培应董胜张边亚琳
Owner CHINA JILIANG UNIV
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