Application of two gliotoxins in preparation of biopesticide

A technology of gliotoxin, biopesticide, applied in the field of agriculture and biology

Inactive Publication Date: 2017-06-13
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] But so far, there are no reports about the anti-phytopathogenic fungi of these two compounds in the literature at home and abroad.

Method used

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  • Application of two gliotoxins in preparation of biopesticide
  • Application of two gliotoxins in preparation of biopesticide
  • Application of two gliotoxins in preparation of biopesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The inhibitory effects of compounds 6-deoxy-5a,6-didehydrogliotoxin and 5a,6-didehydrogliotoxin on Gleospora anthracnose and Alternaria were determined by filter paper method.

[0021] Compounds 6-deoxy-5a, 6-didehydrogliotoxin and 5a, 6-didehydrogliotoxin were dissolved in DMSO and diluted to a concentration of 2 mg / mL.

[0022] Insert the phytopathogenic fungus cakes with a diameter of 4 mm in the center of the plate containing PDA medium, place 4 pieces of filter paper evenly around the cake, and drop 5 μL of extract on the filter paper as a sample group, replace the compound with DMSO The extract was used as blank control. Place in an incubator at 28°C for 3 to 5 days, record the growth diameter of the bacteria cake with the cross measurement method, and calculate the inhibition rate according to the following formula.

[0023] Inhibition rate (%)=(1-sample group pure growth amount / control group pure growth amount)×100%

[0024] The experimental results are shown ...

Embodiment 2

[0029] The EC of compounds 6-deoxy-5a, 6-didehydrogliotoxin and 5a, 6-didehydrogliotoxin against plant pathogenic fungi G. anthracnose and Alternaria were determined by filter paper method 50 value.

[0030] Compounds 6-deoxy-5a,6-didehydrogliotoxin and 5a,6-didehydrogliotoxin were diluted to 800, 400, 200, 100, 50, 25, 12.5 μg / mL with DMSO. Insert the phytopathogenic fungus cakes with a diameter of 4 mm in the center of the plate containing the PDA medium, place 4 pieces of filter paper evenly around the cake, and drop 5 μL of extract on the filter paper, and replace the sample extract with DMSO. Blank control. Place in a constant temperature box at 28°C for 3 to 5 days, record the growth diameter of the bacteria cake with the cross measurement method, calculate the inhibition rate, and use SigmaPlot 10.0 software to calculate the EC 50 value. The experimental results are shown in Table 2:

[0031] Table 2: EC of compounds against phytopathogenic fungi 50 value

[003...

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Abstract

The invention discloses an application of two gliotoxins in the preparation of a biopesticide. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious inhibition effect on plant pathogenic fungi colletotrichum gloesporioides and alternaria alternate. The compounds 6-deoxy-5a, 6-didehydrogliotoxin, 5a, 6-didehydrogliotoxin have an obvious plant pathogenic fungi inhibition effect and have good development and application prospects. Therefore, a candidate bio-pesticide is provided for research and development of novel plant pathogenic fungi and a scientific basis is provided for the development and utilization of natural active materials with a marine fungus source.

Description

technical field [0001] The invention belongs to the field of agriculture and biotechnology, and specifically relates to the application of gliotoxin compounds 6-deoxy-5a, 6-didehydrogliotoxin and 5a, 6-didehydrogliotoxin in the preparation of biological pesticides and as active ingredients in pesticides. [0002] Background technique: [0003] There are many environments with special natural conditions in the ocean, especially the deep-sea marine environment, which makes marine microorganisms living in the deep sea in a unique physical, chemical and ecological environment. Surrounded by , high salinity and high concentration of toxic substances, they have formed a very special biological structure and metabolic mechanism system. Due to this extreme environment, deep-sea marine fungi can produce metabolites with novel structures and unique activities, which have great application value and can provide excellent germplasm resources for the development of biopesticides. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00
CPCA01N43/90
Inventor 李浩华孙章华章卫民
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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