Harmless treatment method for gliotoxin mushroom dregs

A technology of harmless treatment and gliomycin, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve pollution and other problems, achieve the effect of solving environmental pollution, simple production process, and reducing residues

Inactive Publication Date: 2017-06-06
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the gliomycin residue is an ideal nutrient component in the gliomycin fermentation medium, which can be recycled in the gliomycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Get 200kg of gliomycin slag, water content 75%, sprinkle 1kg of calcium oxide, mix evenly, the pH value of the material after testing is 8.52, turn the pile once a day, no gliomycin content in the slag can be detected on the 6th day; Add 10kg of corn flour and mix evenly, and adjust the moisture of the material between 50-60%.

[0030] Bacillus natto ACCC19833 was inserted into beef extract peptone medium for fermentation at 35°C in a slant culture mode for 18 hours, and then inoculated into Bacillus natto fermentation medium,

[0031] Medium composition: soybean meal: 18g; white sugar: 18g; calcium carbonate: 9g; sodium chloride: 0.54g; potassium chloride: 0.54g; magnesium sulfate 0.54g; Iron: 0.054g, water 1L; pH7.5, 121°C for 30mins, cultured at 35°C for 48h, the number of Bacillus natto prepared was 5.2 billion / mL.

[0032] Saccharomyces cerevisiae ACCC20039 was inserted into the wort medium on a slant at 28°C for activation, and then inoculated into the fermentati...

Embodiment 2

[0036] Get 1000kg of gliomycin slag, water content 80%, sprinkle 5kg of calcium oxide, mix evenly, the pH value of the material after testing is 8.52, turn the pile once a day, no gliomycin content can be detected in the 7th day; Add 20kg of corn flour and mix evenly, and adjust the moisture of the material between 50-60%.

[0037] Bacillus natto ACCC10614 was inserted into the beef extract peptone medium for fermentation at 35°C in a slant culture mode for 18 hours, and then inoculated into the fermentation medium of Bacillus natto,

[0038]Medium composition: soybean meal: 18g; white sugar: 18g; calcium carbonate: 9g; sodium chloride: 0.54g; potassium chloride: 0.54g; magnesium sulfate 0.54g; Iron: 0.054g, water 1L; pH7.5, 121°C for 30mins, cultured at 35°C for 48h, the number of Bacillus natto prepared was 4.5 billion / mL.

[0039] Saccharomyces cerevisiae ACCC20042 was inserted into the wort medium on a slant at 28°C for activation, and then inoculated into the fermentatio...

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PUM

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Abstract

The invention relates to the field of regeneration and recycling of wastes and provides a harmless treatment method for gliotoxin mushroom dregs. The modified treatment utilization method of the gliotoxin mushroom dregs comprises the following steps: treating residual gliotoxin mushroom dregs; by taking the treated mushroom dregs as a main raw material and other agricultural byproducts as auxiliary materials, inoculating a selective multiple microorganism solid to ferment; and then performing drying, crushing and sieving, packaging and the like. The treatment method has the characteristics of being simple in production process, easy to operate, short in treatment time and the like. The proteins of a modified product is greater than or equal to 25%, and can be re-used for fermenting culture of gliotoxin, so that the invention provides a novel path which solves the environmental pollution and waste material recycle in gliotoxin production.

Description

technical field [0001] The invention relates to the field of solid waste recycling, and relates to a harmless treatment method for gliomycin slag. Background technique [0002] Gliomycin is an epipolythiopiperazine diketone toxoid, which is a secondary metabolite of fungi such as Trichoderma. It is a low molecular weight polar sulfur bridge type compound. Weidling and Emerson (1936) first separated this substance from Trichodermalignorum, Weidling named this substance gliotoxin (gliotoxin), and its structure was identified by Bell et al. in 1958. [0003] At present, gliomycin mainly produces agricultural biofungicides through liquid submerged fermentation of Trichoderma viride, and is mainly used for soil-borne diseases such as cotton wilt, rice sheath blight, rape sclerotinia, apple rot, grape anthracnose, pepper anthracnose, etc. prevention and treatment. Because of its good killing and inhibitory effects on soil-borne disease pathogens, it has become a hot research to...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/07C12R1/865
CPCC12P17/18
Inventor 张心青马韵升姚刚刘圣鹏杨传伦王建平车树刚秦培广韩立霞王春
Owner CHAMBROAD CHEM IND RES INST CO LTD
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