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Complex microbial inoculant, method for preparing microbial composite carbon source by using complex microbial inoculant, and application of microbial composite carbon source in sewage treatment

A technology of compound microbial inoculum and compound carbon source, applied in biological water/sewage treatment, microorganism-based method, biological sludge treatment, etc. The effect of increasing the internal surface area of ​​the structure and enhancing the denitrification efficiency

Active Publication Date: 2022-04-29
重庆融极浩瀚生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, by adding additional carbon sources for denitrification, although the purpose of denitrification is achieved, microorganisms will relatively increase CO in the process of life activities and metabolism. 2 The amount of sludge produced will increase, and at the same time increase the amount of sludge produced, which will lead to a vicious circle, which is inconsistent with the concept of "carbon neutrality" advocated by the country

Method used

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  • Complex microbial inoculant, method for preparing microbial composite carbon source by using complex microbial inoculant, and application of microbial composite carbon source in sewage treatment
  • Complex microbial inoculant, method for preparing microbial composite carbon source by using complex microbial inoculant, and application of microbial composite carbon source in sewage treatment
  • Complex microbial inoculant, method for preparing microbial composite carbon source by using complex microbial inoculant, and application of microbial composite carbon source in sewage treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Put Aspergillus fumigatus, Aspergillus oryzae and Candida krusei into the wort of the activation medium with a concentration of 1L / L and 5 Baume degrees, and the pH is 6.0 to activate the strains; put Bacillus licheniformis, gum Put Paenibacillus and Bacillus subtilis into the activation medium LB medium (the formula of LB medium is preferably peptone 10g, sodium chloride 5g, glucose 1.5g, yeast powder 5g, add distilled water to make up 1L), pH 6.8, activate the strain; inoculate the activated bacterial solution into the corresponding culture medium for shaking culture, and then ferment in batches in the fermenter. When the total number of microorganisms > 1x10 8 When CFU / g, the storage tank collects the bacterial liquid of each strain, seals the storage tank, and stores it under the condition of ambient temperature ≤ 20°C; compound the bacterial liquid of Aspergillus fumigatus and Aspergillus oryzae at a volume ratio of 4.5:5.5 Bacterial solution, prepare composite mic...

Embodiment 2

[0060] Put Aspergillus fumigatus, Aspergillus oryzae and Candida krusei into the wort of the activation medium with a concentration of 0.5L / L and 5 Baume degrees, and the pH is 6.5 to activate the strains; Bacillus licheniformis, Put the jelly-like Paenibacillus and Bacillus subtilis into the activated medium LB medium (the formula of the LB medium is preferably peptone 10g, sodium chloride 5g, glucose 1.5g, yeast extract powder 5g, add distilled water to make up 1L), The pH is 7.2, and the bacteria are activated; the activated bacteria are inoculated into the corresponding medium for shaking culture, and then fermented in batches in the fermenter. When the total number of microorganisms > 1 × 10 8 When CFU / g, the storage tank collects the bacterial liquid of each strain, seals the storage tank, and stores it under the condition of ambient temperature ≤ 20°C; compound the bacterial liquid of Aspergillus fumigatus and Aspergillus oryzae at a volume ratio of 4:6 Bacterial soluti...

Embodiment 3

[0064] Put Aspergillus fumigatus, Aspergillus oryzae and Candida krusei into the wort of 1.5L / L activation medium with a pH of 5 degrees Baume, and the pH is 6.5 to activate the strains; Bacillus licheniformis, Put the jelly-like Paenibacillus and Bacillus subtilis into the activated medium LB medium (the formula of the LB medium is preferably peptone 10g, sodium chloride 5g, glucose 1.5g, yeast extract powder 5g, add distilled water to make up 1L), The pH is 7.2, and the bacteria are activated; the activated bacteria are inoculated into the corresponding medium for shaking culture, and then fermented in batches in the fermenter. When the total number of microorganisms > 1 × 10 8 When CFU / g, the storage tank collects the bacterial liquid of each strain, seals the storage tank, and stores it under the condition of ambient temperature ≤ 20°C; compound the bacterial liquid of Aspergillus fumigatus and Aspergillus oryzae at a volume ratio of 5:5 Bacterial solution, prepare composi...

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Abstract

The invention provides a complex microbial inoculant, a method for preparing a microbial composite carbon source by the complex microbial inoculant and application of the microbial composite carbon source in sewage treatment, and relates to the technical field of sewage treatment.The complex microbial inoculant comprises independently packaged composite microbial inoculants A and B. The composite microbial inoculant A comprises aspergillus fumigatus liquid and aspergillus oryzae liquid, the compound microorganism bacterium agent B comprises a bacillus licheniformis bacterium solution, a bacillus mucilaginosus bacterium solution, a bacillus subtilis bacterium solution and a candida krusei bacterium solution; the method for preparing the microbial composite carbon source from the composite microbial agent comprises the following steps: standing and precipitating, removing supernate, repeatedly treating, adding a calcium oxide solution, adjusting the pH value, reacting for 24-48 hours, adjusting the pH value again, mixing with the composite microbial agent B, fermenting, and adding the composite microbial agent A; when the COD (Chemical Oxygen Demand) concentration of the fermentation liquor is greater than or equal to 10000 mg / L, ending the fermentation; the purpose of sludge reduction is achieved, and the denitrification efficiency is further enhanced.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a composite bacterial agent, a method for preparing a microbial composite carbon source, and an application of the microbial composite carbon source in sewage treatment. Background technique [0002] At present, in my country's domestic sewage, especially urban sewage treatment, the proportion of low carbon nitrogen ratio sewage is increasing. One of the reasons is that due to the development of my country's social economy, people's current living habits are very different from the past. At the same time, water habits have also changed. The increase in water consumption for bathing, washing and other aspects has led to an increase in nitrogen and phosphorus content in domestic sewage; secondly, because most cities have not diverted rain and sewage during construction, rainwater will Sewage is diluted to form sewage with a low carbon-to-nitrogen ratio; coupled with the low ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C02F11/02C02F3/34C12R1/68C12R1/69C12R1/10C12R1/125C12R1/07C12R1/72
CPCC12N1/20C12N1/16C12N1/14C02F11/02C02F3/341C02F3/347C02F2303/06
Inventor 杨平李大荣周祥俊刘冬梅
Owner 重庆融极浩瀚生物技术有限公司
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