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Aspergillus tubingensis and method for producing fructooligosaccharides through whole-cell catalysis of aspergillus tubingensis

A technology for Aspergillus tabin and fructooligosaccharide, which is applied in the field of Aspergillus tabinin culture and whole-cell catalytic production of fructooligosaccharide, can solve the problem of high conversion cost of immobilized enzymatic method, complicated separation and purification process, limitation of mass transfer uniformity, etc. problems, to achieve the effect of reducing the investment of the fermenter and its ancillary equipment, the process is simple, and the immobilization step is eliminated.

Inactive Publication Date: 2016-04-13
山东星光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that there are many steps, the enzyme is very easy to be inactivated during the immobilization process, the conversion rate is low, and the uniformity of mass transfer is limited because sucrose and converted products have to pass through the immobilized particles.
In addition, the separation and purification process of the enzym

Method used

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  • Aspergillus tubingensis and method for producing fructooligosaccharides through whole-cell catalysis of aspergillus tubingensis
  • Aspergillus tubingensis and method for producing fructooligosaccharides through whole-cell catalysis of aspergillus tubingensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Activate the Aspergillus tubingensis species stored on the PDA slant, the activation medium is the PDA slant medium, the culture temperature is 30°C, and the culture time is 48h.

[0044] (2) Inoculate the activated spores into the fermentation medium, the inoculum size is 1×10 6 cells / L, at 30°C, with a rotation speed of 160r / min, the bacterial cell solution was cultivated for 72h to make a whole cell solution of Aspergillus tubingensis; the mass concentration of the fermentation medium was 150g / L sucrose, 10g / L yeast extract, 10g / LNaNO 3 , 0.5g / L MgSO 4 , 4g / L Na 2 HPO 4 , 0.01g / L FeSO 4 and 0.01g / L CuSO 4 . Stir to dissolve evenly, sterilize, and cool for later use.

[0045] (3) Collect the whole cell liquid solution of Aspergillus tubingensis obtained in the second step, filter it with a sterile 400-mesh filter cloth, wash with distilled water, and filter it again to obtain whole cells of Aspergillus tubingensis with transformation activity.

[0046] (4...

Embodiment 2

[0050] (1) Activate the strains stored on the PDA slant, the activation medium is the PDA slant medium, the culture temperature is 28°C, and the culture time is 60h.

[0051] The second step: inoculate the activated spores into the fermentation medium, the inoculum size is 1×10 7 cells / L, at 28°C, 200r / min at 200r / min, the cell solution was cultured for 72h to make a whole cell solution of Aspergillus tubingensis; the mass concentration of the fermentation medium was 100g / L sucrose, 8g / L yeast extract, 8g / LNaNO 3 , 0.4g / L MgSO 4 , 6g / L Na 2 HPO 4 , 0.02g / L FeSO 4 and 0.02g / L CuSO 4 . Stir to dissolve evenly, sterilize, and cool for later use.

[0052] Step 3: Collect the whole cell liquid solution of Aspergillus tubingensis obtained in the second step, suction filter it with a sterile 200-mesh filter cloth, wash with distilled water, and filter it again to obtain whole cells of Aspergillus tubingensis with transformation activity.

[0053] The fourth step: according t...

Embodiment 3

[0057] Step 1: Activate the strains stored on the PDA slant, the activation medium is the PDA slant medium, the culture temperature is 32° C., and the culture time is 36 hours.

[0058] The second step: inoculate the activated spores into the fermentation medium, the inoculum size is 1×10 8 cells / L, at 32°C, the rotation speed is 180r / min under the condition of culturing for 60h to make the whole cell solution of Aspergillus tubingensis; the mass concentration of the fermentation medium is 100g / L sucrose, 5g / L yeast extract, 5g / LNaNO 3 , 0.8g / L of MgSO 4 , 6g / L Na 2 HPO 4 , 0.03g / L FeSO 4 and 0.03g / L CuSO 4 . Stir to dissolve evenly, sterilize, and cool for later use.

[0059] Step 3: Collect the whole cell liquid solution of Aspergillus tubingensis obtained in the second step, suction filter it with a sterile 200-mesh filter cloth, wash with distilled water, and filter it again to obtain whole cells of Aspergillus tubingensis with transformation activity.

[0060] Th...

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Abstract

The invention discloses aspergillus tubingensis and a method for producing fructooligosaccharides through whole-cell catalysis of the aspergillus tubingensis. The aspergillus tubingensis is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on November 16th, 2015, and the preservation number is CGMCC No.11650. The method for producing fructooligosaccharides through whole-cell catalysis of the aspergillus tubingensis includes the following steps that 1, aspergillus tubingensis whole cells are prepared from aspergillus tubingensis Xg171.21; 2, the aspergillus tubingensis whole cells are used for catalyzing saccharose to produce fructooligosaccharides. The content (accounting for total solids) of obtained fructooligosaccharides is larger than or equal to 55% through high performance liquid chromatography detection on obtained fructooligosaccharides. The process is simple, operation is convenient, enzymatic activity is high, conversion time is short, conversion efficiency is high, and high industrial value is achieved.

Description

Technical field [0001] The present invention is a food biotechnology field, which involves the production method of hybrid fructose, which specializes in the cultivation of a Tabion mold culture and its whole cell catalyzed to produce hypocrutonite sugar. Background technique [0002] Polycanose, also known as Fructooligosaccharides (abbreviated as FOS), is combined with 1 to 3 fruit sugar groups to combine with β ~ 1,2 glycoside bonds with sucrose, to generate cane fruit trianga, facade fruit triple sugar, and sugar, and sugar, and sugar, and sugar, sugar, sugar, and sugar.Mixture of pork fruit five sugar.FOS has the proliferation of probiotics in the intestinal, such as bisidobacterium, detoxifying the intestinal tract, improving human immunity, improving lipid metabolism, preventing cavities, and not being absorbed and utilized by digestive tract.Widely used in health food. [0003] At present, there are two ways to produce low polyfront sugar with sucrose: (1) Deep fermentati...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12P19/00C12R1/66
CPCC12P19/00C12P19/04C12N1/145C12R2001/66
Inventor 曹永兴潘永胜李宁周焕霞钟耀华宁占国赵占利任红蕾
Owner 山东星光生物科技有限公司
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