Ultralow-molecular-weight dendrobium polysaccharides as well as preparation and application thereof

A technology of Dendrobium officinale polysaccharide and Dendrobium officinale can be applied to medical preparations containing active ingredients, organic active ingredients, cosmetic preparations, etc., and can solve the problems of affecting the structure and activity of polysaccharides, residual organic solvents, harmful to human health, etc. Meet the requirements of safe, non-toxic, mild extraction conditions, and excellent transdermal absorption

Active Publication Date: 2018-11-16
山东朵拉朵尚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acid-base method is easy to break some glycosidic bonds of polysaccharide molecules, which seriously affects the structure and activity of polysaccharides.
Sevage method, trichloroacetic acid method, trifluorotrichloroethane method and other methods use a lot of toxic chemical reagents. These methods inevitably have the problem of organic solvent residue, which is not only harmful to human health, but also has potential safety hazards.

Method used

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  • Ultralow-molecular-weight dendrobium polysaccharides as well as preparation and application thereof
  • Ultralow-molecular-weight dendrobium polysaccharides as well as preparation and application thereof
  • Ultralow-molecular-weight dendrobium polysaccharides as well as preparation and application thereof

Examples

Experimental program
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preparation example Construction

[0053] The preparation step of PDA solid medium in the embodiment is: fresh potato is washed and peeled, takes by weighing 200g potato and cuts into pieces, adds water and boils (boiling 30min), utilizes 300 order filter cloth to filter residue, adds agar 20g in filtrate, Glucose 20g, add water to 1L, sterilize at 120°C for 20min, cool down and store for later use.

[0054] The configuration method of Sabouraud's liquid medium is: 40.0g glucose, 10.0g peptone are dissolved in artificial seawater (artificial seawater formula: NaCl 20g, MgSO 4 ·7H 2 O 1g, CaCl 2 2H 2 O 1g, KCl 0.5g, NaHCO 3 0.2g, KBr0.1g, H 3 BO 3 0.01mg, distilled water was added to 1L), and the volume was adjusted to 1L, and then sterilized at 120°C for 20min and stored for later use.

[0055] The disposition method of described nichloramine 18% glycerin medium is: peptone 5.0g, anhydrous glucose 10.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, nicloramine 0.002g, chloramphenicol 0.1g, glycerin 200g, di...

Embodiment 1

[0057] (1) Activation and expansion of strains: Dissolve Aspergillus tubingensis in sterile water to make a bacterial suspension, pipette 3 μL of the bacterial suspension and streak on the surface of the PDA solid medium, activate the culture, and cultivate at a temperature of 30°C. Time 48h, obtain the activated Aspergillus tubingensis bacterial classification (concentration after activation is 1 * 10 4 CFU / mL); Inoculate the activated Aspergillus tubingensis strain into a fermentation shaker flask containing Sabouraud's liquid medium with 1% inoculum amount to carry out expansion cultivation. The cultivation temperature is 30° C. and the rotation speed is 150 r / min. , to obtain the fermented seed liquid of Aspergillus tubingensis, the concentration of the strain was 1×10 7 CFU / mL;

[0058] (2) raw material processing of Dendrobium candidum: take 4kg fresh Dendrobium candidum, pulverizer breaks up, juice is put into 100L fermenter together with slag, injects 96kg Sabouraud l...

Embodiment 2

[0063] (1) Strain activation and expansion: Dissolve Aspergillus tubingensis in sterile water to make a bacterial suspension, pipette 4 μL of the bacterial suspension in DRBC agar medium for activation, culture temperature 28 ° C, culture time 60 h, to obtain The activated Aspergillus tubingensis species (concentration of 1.5 × 10 5 CFU / mL); Inoculate the activated Aspergillus tubingensis strain into the fermentation shaker flask containing Sabouraud liquid medium with 1% inoculum amount to carry out expansion culture, the culture temperature is 28 ℃, the rotating speed 170r / min, after cultivating 60h , to obtain the fermented seed liquid of Aspergillus tubingensis, the concentration of the strain was 1.0×10 8 CFU / mL;

[0064] (2) Dendrobium candidum raw material processing: take 5kg fresh Dendrobium candidum, pulverizer breaks up, juice is put into fermenter together with slag, injects 95kg Sabouraud's liquid culture medium, makes the mass fraction of Dendrobium candidum be ...

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Abstract

The invention belongs to the technical field of dendrobium polysaccharide preparation and discloses ultralow-molecular-weight dendrobium polysaccharides as well as preparation and application thereof.The method comprises the following steps: (1) inoculating an activated aspergillus tubingensis strain into a liquid seed culture medium, and culturing to obtain seed liquid; (2) fetching the seed liquid and inoculating into a fermentation culture medium of a fermentation tank; adjusting the pH of the liquid in the fermentation tank to 4.0-7.0; fermenting at 25-35 DEG C to obtain fermented liquid,wherein the fermentation culture medium contains broken herba dendrodii officinalis; (3) inactivating the fermented liquid; (4) purifying and concentrating the inactivated fermented liquid, adding anexcipient, performing high-pressure homogenization, and drying to obtain the dendrobium polysaccharides. The method disclosed by the invention is simple, safe and pollution-free, and the yield and purity of the polysaccharides are high; in the obtained dendrobium polysaccharides, the polysaccharides with less than 10kDa of weight-average molecular weight account for 98% or above of total polysaccharides; therefore, the obtained dendrobium polysaccharides are ultralow-molecular-weight dendrobium polysaccharides.

Description

technical field [0001] The invention belongs to the technical field of dendrobium polysaccharide preparation, and in particular relates to an ultra-low molecular weight dendrobium officinale polysaccharide and its preparation method and application. Background technique [0002] Dendrobium officinale kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae, rich in polysaccharides, dendrobines, polyphenols, phenanthrenes, joints, xenone compounds, amino acids and trace elements, etc. Bioactive substances are traditional Chinese herbal medicines in my country. According to phytochemical and phytopharmacological studies, the main active ingredient of Dendrobium candidum is polysaccharides, which have the functions of immune regulation, anti-tumor, anti-fatigue, anti-allergic and anti-inflammatory, anti-oxidation, anti-aging, and moisturizing. Based on the excellent biological activity of Dendrobium officinale polysaccharides, Dendrobium polysaccharides ...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23L33/125A61K8/60A61K31/715C12R1/66
CPCA61K8/60A61K31/715A23L33/125C12P19/04
Inventor 周心慧程建华黄桂州周健邓燕柠付尉芳张定宇张继勇
Owner 山东朵拉朵尚生物科技有限公司
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