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Method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi

A technology for white rot fungi, printing and dyeing wastewater, applied in chemical instruments and methods, textile industry wastewater treatment, water pollutants, etc., can solve the problems of low biodegradability, deep color of printing and dyeing wastewater, and high COD, and achieve a complete degree of mineralization. , good effect, high decolorization efficiency

Inactive Publication Date: 2015-07-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Printing and dyeing wastewater has deep color, high COD, and low biodegradability, so the main problem that needs to be solved in the production of printing and dyeing textiles and dyes has always been its decolorization treatment
At present, the co-immobilization of white rot fungi for the treatment of printing and dyeing wastewater has not been reported

Method used

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  • Method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi
  • Method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi
  • Method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Comparison of different co-immobilization carriers of embodiment 1

[0015] 1) Preparation of immobilized materials: Take polyurethane foam, nylon cloth, porous ceramsite, polyester fiber, and metal steel wool balls, soak them in distilled water, wash them, dry them, and store them.

[0016] 2) Preparation of white rot fungus seed liquid: white rot fungus Pleurotus eryngii (Pleurotus eryngii ～Co007 ) inoculated in the sterilized liquid medium, cultured with shaking at 25° C. to 28° C. for 3 to 5 days, and used as seed liquid for later use.

[0017] 3) Preparation of immobilized white-rot fungus: preparing a liquid medium, and adding the immobilized carrier into it according to 5% of the total volume of the medium. The prepared seed solution is inserted into the white rot fungus culture system according to 10% of the medium volume, and cultivated at 28°C and 150r / min for 5-7 days, and the immobilized bacteria are obtained after the mycelium covers the immobilized materi...

Embodiment 2

[0022] The determination of embodiment 2 co-solidification white rot fungus

[0023] 1) Preparation of white rot fungus seed solution: Pleurotus eryngii ～Co007 ), Pleurotus ostreatus, Coriolus versicolor, Phanerochaete chrysosporuim, Bjerkandera adusta, Schizophyllum commune, and Versicolor (Pycnoporus cinnabarinus) 7 kinds of white rot fungi were inoculated in the sterilized liquid medium, cultured with shaking at 25°C-28°C for 3-5 days, and used as seed liquid.

[0024] 2) Preparation of immobilized material: make polyurethane foam 1cm 3 ~2cm 3 Cube, sphere, polyhedron, soak in distilled water, wash, dry and save.

[0025] 3) Preparation of immobilized white-rot fungus: prepare a liquid medium, add polyurethane foam to it according to 5% of the total volume of the medium, and sterilize it for later use. Insert the prepared seed liquids of different white-rot fungi into the liquid medium with immobilized carrier (the ratio of white-rot fungi is not limited to each other) ...

Embodiment 3

[0035] Example 3 Repeated decolorization of dyes by co-immobilized white rot fungi

[0036] Pleurotus eryngii ～Co007 ) and Coriolus versicolor (Coriolus versicolor) were inoculated into the sterilized liquid medium, cultured with shaking at 25°C-28°C for 3-5 days, and used as seed liquid for later use. Set the volume to 1cm 3 ~2cm 3 Polyhedral metal steel wool balls, soaked in distilled water, washed and dried. To prepare a liquid medium, add metal steel wool balls to it according to 20% of the total volume of the medium, and sterilize it for later use. Put the prepared seed solution of different white rot fungi into the liquid medium with immobilized carrier according to 15% of the medium volume, and cultivate at 28°C and 150r / min for 5-7 days, until the mycelium is covered and fixed Immobilized bacteria were obtained after materialization.

[0037] The co-immobilized fungal cells prepared above were used for repeated degradation and decolorization of different printing ...

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Abstract

The invention relates to wastewater treatment application technologies and aims to provide a method for decolorizing printing and dyeing wastewater by adopting co-immobilized white-rot fungi. The method comprises the following steps: inoculating a seed solution containing any two or more white-rot fungi into a liquid culture medium with an immobilization carrier at a volume ratio of 5-20%, and then performing shake culture at 25-30 DEG C for 5-7 days; after covering the immobilization carrier with thalli, performing separation to obtain a carrier fixed with the white-rot fungus thalli, and putting the carrier into the printing and dyeing wastewater, thereby realizing decolorization treatment. The method fully utilizes enzymes generated by the white-rot fungi with different species and complementary enzyme systems to degrade and decolorize the printing and dyeing wastewater, can realize complete degradation of printing and dyeing wastewater pollutants, and can realize repeated decolorization. The method can be used for decolorization treatment of the printing and dyeing wastewater containing dyes with different structure types, and is high in decolorization efficiency. As the pollutants are degraded by the white-rot fungi, degradation products do not cause secondary pollution to a water body.

Description

technical field [0001] The invention belongs to the technical field of wastewater treatment application, and in particular relates to a method for decolorizing printing and dyeing wastewater by co-immobilizing white-rot fungal cells. Background technique [0002] Printing and dyeing wastewater refers to the wastewater discharged from the production and processing of cotton, hemp, chemical fibers and dyes. It produces a large amount. Taking the processing of 1 ton of textiles as an example, 100 to 200 tons of water is needed, and 80 to 90% of it becomes waste water. The main organic pollutants contained in printing and dyeing wastewater are dyes that cause their high chroma; in addition, they also contain a large amount of inorganic salts, sulfides, and various organic compounds with biological toxicity or three properties. Printing and dyeing wastewater has deep color, high COD, and low biodegradability. Therefore, the main problem that needs to be solved in printing and dy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/30
CPCC02F3/347C02F2101/308C02F2103/30
Inventor 陈敏石艳玲
Owner ZHEJIANG GONGSHANG UNIVERSITY
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