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Preparation method and application of immobilized algal toxin degrading bacteria

A technology for degrading bacteria and algae toxins, applied in the fields of water treatment and environmental pollution control, can solve the problems of high cost and secondary pollution of membrane filtration, achieve good biocompatibility, increase degradation rate, and reduce microbial loss

Active Publication Date: 2013-12-11
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physical and chemical methods have their own limitations in practical application, such as the chemical oxidation method will produce secondary pollution, and the cost of the membrane filtration method is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Preparation of immobilized algal toxin-degrading bacteria

[0019] The first step: immobilized carrier - activated carbon fiber pretreatment: the activated carbon fiber material is gently washed several times with ultrapure water to remove attached impurities, put it into a beaker, add ultrapure water to boil for 1.0h, and then use a mass concentration of 10 % hydrochloric acid overnight, take it out, wash it with ultra-pure water until it is neutral, put it in an oven, bake it at 140°C until it reaches a constant weight, take it out, and put it in a dry airtight container for later use.

[0020] The second step: immobilized algal toxin-degrading bacteria: configure nutrient medium, the nutrient medium is selected from LB medium, and its composition is tryptone 10.0g, yeast extract 5.0g, sodium chloride 10.0g, distilled water 1000mL, adjust the pH to 7.0; Weigh a certain mass (according to the ratio of 10g / L) of activated carbon fiber material that has been t...

Embodiment 2

[0022] Example 2 Application of immobilized cyanotoxin-degrading bacteria in the process of extracting and purifying phycobiliproteins from cyanobacteria blooms to remove microcystins.

[0023] 1) Collect and salvage cyanobacteria from eutrophic water bodies, freeze-thaw, salt out, two-phase extraction, ultrafiltration, concentration and purification to obtain phycocyanin, and use solid phase extraction-high performance liquid chromatography to determine the extraction and purification process of phycocyanin The waste liquid and the content of microcystin contained in the waste residue show that microcystin mainly exists in the filtrate of ultrafiltration, and the concentration is very high, among which MC-LR reaches 11.2mg / L.

[0024] 2) Add the immobilized algal toxin-degrading bacteria obtained in Example 1 to the ultrafiltrate containing microcystin-LR in step 1), and take a sample after 3 days to measure the treatment effect. The result is as follows:

[0025] 1. The rem...

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PUM

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Abstract

The invention discloses a preparation method and an application of immobilized algal toxin degrading bacteria. The preparation method of the immobilized algal toxin degrading bacteria comprises the steps: selecting activated carbon fibers as an immobilization material, after carrying out washing, boiling, hydrochloric acid soaking, drying and other pretreatment steps of the immobilization material, adding into an LB culture medium according to a certain specific value, then inoculating a bacterial strain for degrading microcystins (MCs), and carrying out immobilized culture to obtain the immobilized algal toxin degrading bacteria. In the application of the immobilized algal toxin degrading bacteria, while the activated carbon fibers adsorb algal toxins, the algal toxin degrading bacteria immobilized on the activated carbon fibers can degrade the algal toxins, can greatly shorten the time of degradation of the MCs, and simultaneously can biologically regenerate the activated carbon fibers. The bacterial strain for degrading the MCs has the MC-LR degradation rate of up to 91% or more, and is especially suitable for degradation of the MCs generated in a process of extracting phycobiliproteins from water-blooming cyanobacteria.

Description

technical field [0001] The invention relates to the fields of water treatment and environmental pollution control, in particular to an immobilized cyanotoxin-degrading bacterium for removing microcystin produced in the process of extracting phycobiliprotein by a cyanobacteria bloom through a biochemical method and a preparation method thereof. Background technique [0002] In recent years, "water blooms" dominated by Microcystis aeruginosa have occurred every year in Chaohu Lake, sometimes reaching a thickness of several centimeters. Being salvaged, the resource utilization of the salvaged cyanobacteria has become a key research topic. Water bloom cyanobacteria is rich in phycocyanin, which is a sapphire blue natural pigment, which is widely used in food, beverage, medicine, and cosmetic industries, but at present, it is mainly extracted from freshwater cultured spirulina at home and abroad. Phycocyanin is expensive, which limits the application and development of phycocyan...

Claims

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Application Information

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IPC IPC(8): C12N11/14C07K1/00C12R1/085
Inventor 李玉成袁媛吴涓王宁
Owner ANHUI UNIVERSITY
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