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Microbial preparation for degrading zearalenone and preparation method thereof

A technology of zearalenone and microbial preparation, applied in the field of zearalenone-degrading microbial preparation and its preparation, can solve the problem that the degradation product cannot be non-toxic, the activity of ZEN microbial degrading bacteria is low, and it is difficult to realize production and utilization And other issues

Pending Publication Date: 2021-08-31
苏州优利莱生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many research reports that some bacteria, fungi and metabolic enzymes can degrade ZEN, but some microorganisms have low degradation activity, the degrading enzymes are difficult to separate and purify, and the degradation products cannot be non-toxic, etc. In addition, some microorganisms with high degradation activity cannot be directly added into the feed, thus limiting its application in the livestock industry
[0004] Therefore, in order to solve the problem of ZEN pollution in feed and raw materials, and overcome the problems of low activity of existing ZEN microbial degradation bacteria and difficulty in realizing production and utilization, etc.

Method used

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  • Microbial preparation for degrading zearalenone and preparation method thereof
  • Microbial preparation for degrading zearalenone and preparation method thereof
  • Microbial preparation for degrading zearalenone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Inoculate the Stapella strain with the preservation number CGMCC No. 17759 into LB liquid medium (inoculum size: 1%), and culture at 37° C. and 200 r for 24 hours to obtain an activated seed liquid of Stapella once. Continue to inoculate the once-activated seed liquid of Staplepia spp. into LB liquid culture medium (inoculum size 1%), and after culturing at 37° C. and 200 r for 36 hours, the Tapeella bacterium liquid is obtained.

[0025] The LB medium contains (per liter) 10g of peptone, 10g of sodium chloride, 5g of yeast extract powder, the pH value is adjusted to 7.2, and steam sterilization at 121°C for 20min.

[0026] (2) Inoculate the strain of Bacillus velesi with the preservation number CGMCC No. 17328 into LB liquid culture medium (inoculum size 1%), and cultivate it at 37° C. at 200 r for 24 hours to obtain an activated seed liquid of Bacillus velesii once. Continue to inoculate the once-activated Bacillus veleisi seed liquid into the LB liquid medium (in...

Embodiment 2

[0040] (1) Inoculate the Stapella strain with the preservation number CGMCC No. 17759 into LB liquid medium (inoculum size: 1.5%), and culture it at 35° C. and 200 r for 23 hours to obtain an activated seed liquid of Stapella once. Continue to inoculate the once-activated seed solution of Staplepsia into LB liquid medium (inoculum size: 1.5%), and culture it at 39°C and 220r for 36 hours to obtain the Staplesia bacteria solution.

[0041] The LB medium contains (per liter) 10g of peptone, 10g of sodium chloride, 5g of yeast extract powder, the pH value is adjusted to 7.2, and steam sterilization at 121°C for 20min.

[0042] (2) Inoculate the strain of Bacillus velesi with the preservation number CGMCC No. 17328 into LB liquid culture medium (inoculum size: 1.5%), and cultivate it at 39°C and 220r for 25 hours to obtain an activated seed liquid of Bacillus velesii once. Continue to inoculate the once-activated Bacillus veleisi seed liquid into the LB liquid medium (1.5% inoculu...

Embodiment 3

[0056] (1) Inoculate the Stapella strain with the preservation number CGMCC No. 17759 into LB liquid medium (inoculum size: 1.3%), and culture it at 39°C and 220r for 25 hours to obtain an activated seed liquid of Stapella once. Continue to inoculate the once-activated seed solution of Staplepsia into LB liquid medium (inoculum size 1.3%), and after culturing at 35° C. and 200 r for 34 hours, the Tapella bacterium fluid is obtained.

[0057] The LB medium contains (per liter) 10g of peptone, 10g of sodium chloride, 5g of yeast extract powder, the pH value is adjusted to 7.2, and steam sterilization at 121°C for 20min.

[0058] (2) Inoculate the strain of Bacillus velesi with the preservation number CGMCC No. 17328 into LB liquid medium (inoculum size: 1.3%), and culture it at 35° C. and 200 r for 23 hours to obtain an activated seed liquid of Bacillus velesii once. Continue to inoculate the once-activated Bacillus velesii seed liquid into the LB liquid medium (1.3% inoculum si...

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Abstract

The invention relates to the technical field of toxin degradation, and particularly discloses a microbial preparation for degrading zearalenone and a preparation method thereof. The preparation method comprises the steps that Starpa bacteria and bacillus velezensis are cultured respectively to obtain a Starpa bacterial solution and a bacillus velezensis solution, then the bacillus velezensis bacterial solution and the Starpa bacterial solution are mixed to obtain a compound bacteria bacterial solution, and finally the compound bacteria bacterial solution is put into a compound bacteria culture medium for mixed fermentation to obtain the microbial preparation. The microbial preparation has high degradation rate on zearalenone in the feed, and the fermentation inoculum is safe and environment-friendly, and can be directly added into the feed as a feed additive.

Description

technical field [0001] The invention belongs to the technical field of toxin degradation, and in particular relates to a microbial preparation for degrading zearalenone and a preparation method thereof. Background technique [0002] Zearalenone (Zearalenone, ZEN), also known as F-2 toxin, is a secondary metabolite produced by Fusarium graminearum, Fusarium yellow, Fusarium moniliforme, etc. Found in grains and their by-products. The structures of ZEN and its derivatives are very similar to 17β-estradiol, so they can competitively bind to estrogen receptors and trigger a series of estrogen-like effects. Therefore, when ZEN enters the animal body, it will cause damage to the reproductive system of the animal. In addition, ZEN also has genotoxicity, cytotoxicity, immunotoxicity, and carcinogenicity. ZEN exists in a large amount in moldy corn, sorghum, wheat and other crops, and is one of the pollutants in food and feed worldwide. According to the estimates of the Food and Ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/12A23L5/20C12R1/01C12R1/07
CPCC12N1/20A23K10/12A23L5/28
Inventor 范婵娟王馨悦陈远庆
Owner 苏州优利莱生物科技有限公司
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