A kind of alginolytic/algal toxin degradation double-effect engineering bacteria y1 and its construction method

A technology of algal toxins and engineering bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as difficulties, algal toxin pollution, and inconsistent environmental requirements
CN103525746BActive Publication Date: 2016-01-27ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI HUANGHE WATER RESOURCE POLYTRON TECH INC
Publication Date
2016-01-27

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Abstract

The invention discloses an alga lysing / algal toxin degradation double-effect engineering strain Y1 and a construction method thereof, belonging to the field of microbiology for treatment of blue-green algae. Fusion is performed on parent strains with alga lysing and algal toxin degradation functions by utilizing a protoplast fusion technology to construct the double-effect engineering strain with excellent traits of parents, namely Bacillussphaericus with the collection number of CGMCCNO. 7519. The alga lysing and the derived algal toxin pollution problem are synchronously solved. The protoplast fusion rate can achieve 31.97% under optimal conditions, and the double-effect engineering strain has certain guide effects on solving the problems of blue-green alga bloom and the derived MC-LR (microcystin-LR) pollution and constructing the engineering strain integrating the MC-LR degradation function and the alga lysing property into a whole by performing a protoplast fusion technology, thereby providing reference for achieving the effect on treating both the root course and the symptoms for solving the large-area blue-green alga bloom problem.
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Description

technical field

[0001] The invention belongs to the field of microbiology of cyanobacteria treatment, and in particular relates to a method for constructing alginolytic / algal toxin-degrading double-effect engineering bacteria. Background technique

[0002] Excessive N, P and other substances enter the water environment, and the nutrients received by the water body exceed its own purification capacity, resulting in the excessive reproduction of cyanobacteria and other phytoplankton, and the accumulation of buoyant planktonic cyanobacteria on the surface of the water body, resulting in the phenomenon of "bloom". In addition, certain cyanobacteria, such as Microcystis aeruginosa, produce a large amount of algal toxins stored in the cells during the reproduction process, and are released with the death of the algal cells, and exist in the water in a soluble state, which seriously threatens the health of animals and humans. . There are more than 80 types of algae toxins that hav...

Claims

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