A novel Sphingosine strain and its application in the field of microcystin degradation and detoxification

Active Publication Date: 2019-10-18
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physical methods can usually only transfer microcystins in the water environment to other places, and there are still potential safety hazards, and the treatment process is cumbersome and expensive; although chemical methods are effective in dealing with microcystins, they are likely to create an environment of toxic and harmful substances. Residues, leading to the occurrence of secondary pollution
Biological metabolic transformation is one of the main ways to remove microcystins in the water environment. Some special bacteria in the water environment have the advantages of high efficiency, low cost, convenient operation, no secondary pollution, and ecological restoration. , is an environmentally friendly microcystin removal technology with great application prospects, and can be used as a priority method for removing microcystins in water environments. In recent years, it has attracted great attention from scholars at home and abroad. Microcystin degradation efficiency of microorganisms is relatively low, such as: when Pseudomonas putida degrades microcystin MC-LR, the rate is only 0.001μg / ml / h (0.5mg / lMC-LR degradation rate reaches 52.6 after 10 days %)

Method used

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  • A novel Sphingosine strain and its application in the field of microcystin degradation and detoxification
  • A novel Sphingosine strain and its application in the field of microcystin degradation and detoxification
  • A novel Sphingosine strain and its application in the field of microcystin degradation and detoxification

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Embodiment

[0037] A Sphingopyxissp. YF1 strain provided by the present invention is named as Sphingopyxissp. YF1, and it is preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short). The preservation number of the General Microbiology Center of the Culture Collection Management Committee is CGMCC No: 16341, and the preservation time is August 27, 2018. The address of the preservation unit is located at the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0038] 1. Screening of bacterial strains

[0039] (1) Sample collection

[0040] 5g of sludge was collected from Taihu Lake with severe eutrophication, put into a sampling bottle, retrieved, and stored in a 4°C refrigerator for later use.

[0041] (2) Culture medium preparation

[0042] Beef extract peptone medium (g / L): 5g beef extract, 10g peptone, 5g NaCl, pH 7.0-7.2.

[0043] MSM inorganic salt mediu...

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Abstract

The invention discloses a new strain of Sphingopyxis sp., which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee. It is named Sphingopyxis sp. YF1 (Sphingopyxis sp. YF1), and the preservation number is CGMCC No: 16341, which was deposited on August 27, 2018. The strain has a strong degrading effect on microcystin, can be applied to control microcystin pollution, and also provides a new strain for the field of microcystin degradation. The invention also discloses the application of the sphingosine strain in the field of microcystin degradation and detoxification. The sphingosine YF1 is used to degrade and detoxify the microcystins, the degradation rate is high, and the microcystins can be completely degraded , can also remove the toxicity of microcystin and its degradation products, solve the problem that the current strains cannot completely remove the toxicity of microcystin, and have strong practical value and application value.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a novel sphingosine strain and its application in the field of microcystin degradation and detoxification. Background technique [0002] In recent years, with the change of human life style, the rapid development of urban industry and the increase of urban population, a large amount of domestic sewage and industrial wastewater are poured into the lake, resulting in the gradual eutrophication of the lake. The problem of cyanobacterial blooms caused by eutrophication of the water environment has become increasingly serious in my country. Freshwater lakes such as Dianchi Lake, Taihu Lake and Chaohu Lake have high concentrations of cyanobacteria blooms that break out every summer and autumn, and cyanobacteria blooms often produce secondary metabolites microcapsules Algal toxins pose serious hazards to aquatic ecosystems and human health. [0003] Microcystin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 杨飞张宪丁萍黄飞羽冯海
Owner CENT SOUTH UNIV
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