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Bacillus velezensis for simultaneously degrading zearalenone and aflatoxin and application of Bacillus velezensis

A technology of zearalenone and aflatoxin, which is applied in the field of agricultural biology and can solve problems such as mycotoxin pollution

Active Publication Date: 2020-02-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to screen beneficial bacteria that simultaneously degrade zearalenone and aflatoxin from a large number of natural samples, thereby solving the problem of mycotoxin pollution in grain, feed and food

Method used

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  • Bacillus velezensis for simultaneously degrading zearalenone and aflatoxin and application of Bacillus velezensis
  • Bacillus velezensis for simultaneously degrading zearalenone and aflatoxin and application of Bacillus velezensis
  • Bacillus velezensis for simultaneously degrading zearalenone and aflatoxin and application of Bacillus velezensis

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Screening of simultaneously degrading zearalenone and aflatoxin strains

[0031] 29 samples including soil, moldy feed, pig and chicken intestinal waste were collected for screening microorganisms that degrade zearalenone and aflatoxin at the same time. 1 g of sample was dissolved in sterile PBS buffer, spread on LB plates, and incubated at 37°C for 24h. Randomly pick 20 single colonies from each plate, and streak and purify 3 times on LB plates to obtain purified strains. After the isolated and purified strains were cultured in LB medium at 37°C for 24 hours, the degradation rate of each strain on ZEN and AFB1 was detected.

[0032] The reaction conditions were as follows: 990 μL strain fermentation broth and 10 μL ZEN (200 μg / mL), incubated at 37 °C for 48 h, 990 μL sterile PBS and 10 μL ZEN (200 μg / mL) were used as controls, after the reaction was completed, an equal volume of methanol was added to terminate the reaction, and The concentration of ZEN was ...

Embodiment 2

[0036] Example 2 Degradation effect of Bacillus Velez ANSB01E on ZEN in naturally moldy corn

[0037] First, crush ZEN moldy corn, weigh 10g and resuspend in 40mL distilled water, sterilize at 121°C for 15min, detect the concentration of ZEN in the corn flour extract to be 0.74μg / mL, and inoculate 10 % of Bacillus Velez ANSB01E or LB medium, after incubation at 37°C for 48 hours, samples were taken at 0, 12, 24 and 48 hours of incubation, and the ZEN content was detected by HPLC.

[0038] see results Figure 4 , the degradation rate of Bacillus Velez ANSB01E to ZEN in naturally moldy corn was 73%.

Embodiment 3

[0039] Example 3 Degradation of zearalenol, zearalenol, AFM1 and AFG1 by Bacillus Velez ANSB01E

[0040] Dissolve zearalenol, zearalenol, AFM1 and AFG1 in dimethyl sulfoxide respectively, and carry out the test according to the following reaction system: 990 μL Bacillus velei fermented broth, 10 μL toxin, zearalen in the system The concentration of alcohol or zearalenol was 10 μg / mL, and the concentration of AFM1 or AFG1 was 1 μg / mL; the reaction between PBS and toxin was used as the control; after reacting at 37°C for 48 hours, 1 mL of methanol was added to terminate the reaction, and high-performance liquid chromatography was used to detect each Toxin content. Table 1 shows the efficiency of Bacillus Velez for degrading zearalenol, zearalenol, AFM1 and AFG1.

[0041] Table 1 Efficiency of Bacillus Veles degrading zearalenol, zearalenol, AFM1 and AFG1

[0042]

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Abstract

The invention relates to the technical field of agricultural biologics and in particular relates to Bacillus velezensis ANSB01E CGMCC No. 17328 and application thereof. The Bacillus velezensis provided by the invention is capable of simultaneously and efficiently degrading zearalenone and aflatoxin, and degradation rates upon g zearalenone and aflatoxin are both up to 90% or greater 48 hours laterafter reactions. The Bacillus velezensis provided by the invention is high in toxin degradation efficiency, good in specificity, mild in action effect, free of damage to nutrient components in feedsand applicable to biological detoxification of mycotoxins in cereals, feeds and foods.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a Bacillus velezensis ANSB01E capable of simultaneously degrading zearalenone and aflatoxin and an application thereof. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by fungi such as Aspergillus spp., Fusarium spp., and Penicillium spp. during their growth. At present, there are more than 300 kinds of mycotoxins that are toxic to humans and animals, among which, aflatoxin (Aflatoxin), zearalenone (Zearalenone), trichothecenes (Trichothecenes), ochre Aspergillus toxins (Ochratoxin) and fumonisins (Fumonisins) are the most serious threats to animal husbandry. Traditional physical and chemical elimination methods have shortcomings such as unstable effects, large loss of nutrients, affecting feed palatability, and difficulty in large-scale production, so they cannot be widely used in actual production. Microbial and biological enzyme deg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20C12R1/07
CPCC12N1/20A23L5/28A23L5/25C12R2001/07C12N1/205
Inventor 赵丽红马秋刚计成郭永鹏
Owner CHINA AGRI UNIV
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