Bacillus velezensis for simultaneously degrading zearalenone and aflatoxin and application of Bacillus velezensis
A technology of zearalenone and aflatoxin, which is applied in the field of agricultural biology and can solve problems such as mycotoxin pollution
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Embodiment 1
[0030] Example 1 Screening of simultaneously degrading zearalenone and aflatoxin strains
[0031] 29 samples including soil, moldy feed, pig and chicken intestinal waste were collected for screening microorganisms that degrade zearalenone and aflatoxin at the same time. 1 g of sample was dissolved in sterile PBS buffer, spread on LB plates, and incubated at 37°C for 24h. Randomly pick 20 single colonies from each plate, and streak and purify 3 times on LB plates to obtain purified strains. After the isolated and purified strains were cultured in LB medium at 37°C for 24 hours, the degradation rate of each strain on ZEN and AFB1 was detected.
[0032] The reaction conditions were as follows: 990 μL strain fermentation broth and 10 μL ZEN (200 μg / mL), incubated at 37 °C for 48 h, 990 μL sterile PBS and 10 μL ZEN (200 μg / mL) were used as controls, after the reaction was completed, an equal volume of methanol was added to terminate the reaction, and The concentration of ZEN was ...
Embodiment 2
[0036] Example 2 Degradation effect of Bacillus Velez ANSB01E on ZEN in naturally moldy corn
[0037] First, crush ZEN moldy corn, weigh 10g and resuspend in 40mL distilled water, sterilize at 121°C for 15min, detect the concentration of ZEN in the corn flour extract to be 0.74μg / mL, and inoculate 10 % of Bacillus Velez ANSB01E or LB medium, after incubation at 37°C for 48 hours, samples were taken at 0, 12, 24 and 48 hours of incubation, and the ZEN content was detected by HPLC.
[0038] see results Figure 4 , the degradation rate of Bacillus Velez ANSB01E to ZEN in naturally moldy corn was 73%.
Embodiment 3
[0039] Example 3 Degradation of zearalenol, zearalenol, AFM1 and AFG1 by Bacillus Velez ANSB01E
[0040] Dissolve zearalenol, zearalenol, AFM1 and AFG1 in dimethyl sulfoxide respectively, and carry out the test according to the following reaction system: 990 μL Bacillus velei fermented broth, 10 μL toxin, zearalen in the system The concentration of alcohol or zearalenol was 10 μg / mL, and the concentration of AFM1 or AFG1 was 1 μg / mL; the reaction between PBS and toxin was used as the control; after reacting at 37°C for 48 hours, 1 mL of methanol was added to terminate the reaction, and high-performance liquid chromatography was used to detect each Toxin content. Table 1 shows the efficiency of Bacillus Velez for degrading zearalenol, zearalenol, AFM1 and AFG1.
[0041] Table 1 Efficiency of Bacillus Veles degrading zearalenol, zearalenol, AFM1 and AFG1
[0042]
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